公开(公告)号：US20220379305A1

公开(公告)日：2022-12-01

申请号：US17825945

申请日：2022-05-26

Applicant: ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

Inventor： Brinda Kodira Cariappa ,  Wayne N. George ,  Sahngki Hong ,  Lewis J. Kraft

IPC: B01L3/00 ,  G03F7/039 ,  G03F7/11 ,  G03F7/038

Abstract: In an example of a method for making a flow cell, a metal material is sputtered over a transparent substrate including depressions separated by interstitial regions to form a metal film having a first thickness over the interstitial regions and having a second thickness over the depressions, the second thickness being about 30 nm or less and being at least ⅓ times smaller than the first thickness. A light sensitive material is deposited over the metal film; and the metal film is used to develop the light sensitive material through the transparent substrate to define an altered light sensitive material at a first predetermined region over the transparent substrate. The altered light sensitive material is utilized to generate a functionalized layer at the first predetermined region or at a second predetermined region over the transparent substrate.

公开(公告)号：US20240418630A1

公开(公告)日：2024-12-19

申请号：US18822004

申请日：2024-08-30

Applicant: ILLUMINA, INC.

Inventor： Sahngki Hong ,  M. Shane Bowen ,  Lewis J. Kraft

IPC: G01N21/05 ,  G03F7/00 ,  G03F7/09 ,  H01L21/02 ,  H01L21/311 ,  H01L31/08

Abstract: One example of a flow cell includes a base support and a multi-layer stack positioned over the base support. The multi-layer stack includes a resin layer positioned over the base support; and a hydrophobic layer positioned over the resin layer. A depression is defined in the multi-layer stack through the hydrophobic material and through a portion of the resin.

公开(公告)号：US20240345475A1

公开(公告)日：2024-10-17

申请号：US18633865

申请日：2024-04-12

Applicant: Illumina, Inc.

Inventor： Krishnarjun Sarkar ,  Sahngki Hong ,  Weixian Xi ,  Brandon Wenning ,  Lewis Kraft ,  Jeffrey Fisher ,  Wayne George ,  Brian Mather

IPC: G03F7/00

CPC classification number: G03F7/0002

Abstract: In some examples, a method includes disposing a first hydrogel within a first recess of a substrate and over a pillar. The substrate may include a second recess in which the pillar is disposed, and a wall separating the first recess from the second recess. While the first hydrogel is disposed within the first recess, the pillar may be removed. After removing the pillar, a second hydrogel may be disposed within the second recess. Also provided herein are nonlimiting examples of manners in which recesses, walls, and patterned hydrogels may be formed.

公开(公告)号：US20240302738A1

公开(公告)日：2024-09-12

申请号：US18667993

申请日：2024-05-17

Applicant: ILLUMINA, INC.

Inventor： Jeffrey S. Fisher ,  Brian D. Mather ,  Maria Candelaria Rogert Bacigalupo ,  Justin Fullerton ,  Ludovic Vincent ,  Lewis J. Kraft ,  Sahngki Hong ,  Boyan Boyanov ,  M. Shane Bowen ,  Sang Park ,  Wayne N. George ,  Andrew A. Brown ,  Dajun Yuan

IPC: G03F7/00 ,  C08F220/56 ,  C08G77/04 ,  C12Q1/6869 ,  G03F7/004 ,  G03F7/075 ,  G03F7/16

CPC classification number: G03F7/0002 ,  C08F220/56 ,  C08G77/04 ,  C12Q1/6869 ,  G03F7/0757 ,  G03F7/165 ,  G03F7/0046

Abstract: An example of a flow cell includes a substrate; a first primer set attached to a first region on the substrate, the first primer set including an un-cleavable first primer and a cleavable second primer; and a second primer set attached to a second region on the substrate, the second primer set including a cleavable first primer and an un-cleavable second primer.

公开(公告)号：US20240210829A1

公开(公告)日：2024-06-27

申请号：US18520093

申请日：2023-11-27

Applicant: ILLUMINA, INC.

Inventor： Jeffrey S. Fisher ,  Sahngki Hong ,  Lewis J. Kraft ,  David Prescott ,  Brandon Wenning ,  Weixian Xi

IPC: G03F7/039 ,  G03F7/038 ,  G03F7/11 ,  G03F7/20

CPC classification number: G03F7/039 ,  G03F7/038 ,  G03F7/11 ,  G03F7/2004

Abstract: In an example method, a positive photoresist is deposited over a substrate that includes depressions separated by interstitial regions. The positive photoresist is exposed to ultraviolet light at an angle that is non-perpendicular, non-parallel, and offset from a surface plane of the depressions such that a first portion of the positive photoresist in each depression remains soluble and a second portion of the positive photoresist in each depression is rendered insoluble. The soluble portions of the positive photoresist are removed, which exposes a first substrate portion in each depression. A first functionalized layer is deposited over the first substrate portion in each depression. The insoluble portions of the positive photoresist are removed, which exposes a second substrate portion in each depression. The second functionalized layer is selectively deposited over the second substrate portion in each depression.

公开(公告)号：US11988957B2

公开(公告)日：2024-05-21

申请号：US18317865

申请日：2023-05-15

Applicant: ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

Inventor： Jeffrey S. Fisher ,  Brian D. Mather ,  Maria Candelaria Rogert Bacigalupo ,  Justin Fullerton ,  Ludovic Vincent ,  Lewis J. Kraft ,  Sahngki Hong ,  Boyan Boyanov ,  M. Shane Bowen ,  Sang Park ,  Wayne N. George ,  Andrew A. Brown

IPC: G03F7/00 ,  C08F220/56 ,  C08G77/04 ,  C12Q1/6869 ,  G03F7/075 ,  G03F7/16 ,  G03F7/004

CPC classification number: G03F7/0002 ,  C08F220/56 ,  C08G77/04 ,  C12Q1/6869 ,  G03F7/0757 ,  G03F7/165 ,  G03F7/0046

Abstract: An example of a flow cell includes a substrate; a first primer set attached to a first region on the substrate, the first primer set including an un-cleavable first primer and a cleavable second primer; and a second primer set attached to a second region on the substrate, the second primer set including a cleavable first primer and an un-cleavable second primer.

公开(公告)号：US20240123448A1

公开(公告)日：2024-04-18

申请号：US18477468

申请日：2023-09-28

Applicant: ILLUMINA, INC.

Inventor： Jeffrey S. Fisher ,  Anthony Flannery ,  Sahngki Hong ,  Brinda Kodira Cariappa ,  Lewis J. Kraft

IPC: B01L3/00

CPC classification number: B01L3/502761 ,  B01L3/502707 ,  B01L2200/0647 ,  B01L2200/12

Abstract: An example of a flow cell includes a substrate and a reaction area defined in or over the substrate. The reaction area includes two angularly offset and non-perpendicular surfaces relative to a planar surface of the substrate, a polymeric hydrogel positioned over at least a portion of each of the two angularly offset and non-perpendicular surfaces; a first primer set attached to the polymeric hydrogel that is positioned over the portion of a first of the two angularly offset and non-perpendicular surfaces; and a second primer set attached to the polymeric hydrogel that is positioned over the portion of a second of the two angularly offset and non-perpendicular surfaces, wherein the first and second primer sets are orthogonal.

公开(公告)号：US20220100091A1

公开(公告)日：2022-03-31

申请号：US17486568

申请日：2021-09-27

Applicant: ILLUMINA, INC.

Inventor： Sahngki Hong ,  Lewis J. Kraft

IPC: G03F7/039 ,  G03F7/20 ,  G03F7/34 ,  G03F7/38 ,  G03F7/038

Abstract: An example flow cell includes a multi-layer stack including a transparent base support; a patterned sacrificial layer over the transparent base support; and a transparent layer over the patterned sacrificial layer. The flow cell further includes first and second functionalized layers over different portions of the transparent layer, wherein at least one of the first and second functionalized layers aligns with a pattern of the patterned sacrificial layer; and first and second primer sets respectively attached to the first and second functionalized layer.