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152 条记录 (耗时 0.053 秒)

    Position dependent recognition of GNN nucleotide triplets by zinc fingers
    23.
    发明申请
    Position dependent recognition of GNN nucleotide triplets by zinc fingers 有权
    锌指的GNN核苷酸三联体的位置依赖性识别

    公开(公告)号：US20080242847A1

    公开(公告)日：2008-10-02

    申请号：US11893341

    申请日：2007-08-15

    申请人： Qiang Liu ,  Edward Rebar ,  Andrew C. Jamieson

    发明人： Qiang Liu ,  Edward Rebar ,  Andrew C. Jamieson

    IPC分类号： C07K14/00

    CPC分类号： C07K14/00 ,  C07K1/00 ,  C07K14/47 ,  C07K14/4702 ,  C07K2299/00 ,  C12N15/102 ,  C12N15/62 ,  C12Q1/6886 ,  C12Q2600/156 ,  G01N33/5308 ,  G01N33/57484 ,  G01N33/6875 ,  G01N2500/04

    摘要： The specificity of binding of a zinc finger to a triplet or quadruplet nucleotide target subsite depends upon the location of the zinc finger in a multifinger protein and, hence, upon the location of its target subsite within a larger target sequence. The present disclosure provides zinc finger amino acid sequences for recognition of triplet target subsites having the nucleotide G in the 5′-most position of the subsite, that have been optimized with respect to the location of the subsite within the target site. Accordingly, the disclosure provides finger position-specific amino acid sequences for the recognition of GNN target subsites. This allows the construction of multi-finger zinc finger proteins with improved affinity and specificity for their target sequences, as well as enhanced biological activity.

    摘要翻译： 锌指结合到三联体或四核苷酸靶位点的特异性取决于锌指在多角蛋白中的位置，并且因此取决于其目标亚位点在较大靶序列内的位置。 本公开提供了锌指氨基酸序列，用于识别具有子部位的5'-最多位置的核苷酸G的相对于靶位点内的亚位点的位置优化的三重态目标亚位点。 因此，本公开提供了用于识别GNN靶亚属的手指位置特异性氨基酸序列。 这允许构建具有改善其靶序列的亲和性和特异性的多指锌指蛋白，以及增强的生物活性。

    Flexible polymer, particles prepared therefrom and process for preparing the same
    24.
    发明申请
    Flexible polymer, particles prepared therefrom and process for preparing the same 有权
    柔性聚合物，由其制备的颗粒及其制备方法

    公开(公告)号：US20080139413A1

    公开(公告)日：2008-06-12

    申请号：US12001956

    申请日：2007-12-12

    申请人： Pingmei Wang ,  Jianhui Luo ,  Yuzhang Liu ,  Huaijiang Zhu ,  Chunming Xiong ,  Qiang Liu ,  Ruyi Jiang ,  Yikun Li ,  Ying Zhang

    发明人： Pingmei Wang ,  Jianhui Luo ,  Yuzhang Liu ,  Huaijiang Zhu ,  Chunming Xiong ,  Qiang Liu ,  Ruyi Jiang ,  Yikun Li ,  Ying Zhang

    IPC分类号： C09K8/588

    CPC分类号： C09K8/516 ,  C08F222/26 ,  C08F236/10 ,  C09K2208/18

    摘要： Disclosed are a flexible polymer, particles made from same, and a process for preparing the particles. This flexible polymer is obtained from copolymerizing monomer (A) and monomer (B), wherein monomer (A) is one or more water-insoluble unsaturated diene monomers; monomer (B) is at least one compound with the general formula of wherein R is C1-C12alkyl, C1-C12 alkyl aryl, C1-C12 alkyl ether or C1-C12alkyl ester. Monomer (A) is in an amount of 60-90% by weight of the total combined weight of monomer (A) and monomer (B). Monomer (B) is in an amount of 10-40% by weight of the total combined weight of monomer (A) and monomer (B). The flexible polymer particles show excellent flexibility, deformability, elasticity as well as stability. They can be used in oilfields in nearby wellbore profile control and in-depth profile control or as in-depth flooding fluid diverting agents. They can also be applied in water shutoff in high temperature and high salinity production wells; preventing chemical channeling in polymer and ASP (alkali-surfactant-polymer) flooding; temporarily plugging in acidization; huff and puff; and preventing in-depth channeling in steam flooding, loss of circulation control and filtration control, and the like.

    摘要翻译： 公开了柔性聚合物，由其制成的颗粒和制备颗粒的方法。 该柔性聚合物由共聚单体（A）和单体（B）得到，其中单体（A）为一种或多种水不溶性不饱和二烯单体; 单体（B）是至少一种具有以下通式的化合物：其中R是C 1 -C 12烷基，C 1 -C 3烷基， C 12 -C 12烷基芳基，C 1 -C 12烷基醚或C 1 -C 12烷基醚或C 1 -C 12烷基醚 >烷基酯。 单体（A）的单体（A）和单体（B）的总组合重量的60-90重量％。 单体（B）的单体（A）和单体（B）的总组合重量的量为10-40重量％。 柔性聚合物颗粒显示出优异的柔韧性，变形性，弹性以及稳定性。 它们可用于油田附近的井筒剖面控制和深度剖面控制，或用作深度淹没液体转向剂。 也可应用于高温高盐生产井的关闭水中; 防止聚合物和ASP（碱 - 表面活性剂 - 聚合物）淹水中的化学通道; 暂时酸化; 气喘吁吁; 并防止蒸汽溢流的深入沟通，流通控制和过滤控制的损失等。

    Corner-pumping method and gain module for solid state slab laser
    29.
    发明申请
    Corner-pumping method and gain module for solid state slab laser 有权
    固态平板激光器的角抽法和增益模块

    公开(公告)号：US20050111510A1

    公开(公告)日：2005-05-26

    申请号：US10719072

    申请日：2003-11-21

    申请人： Mali Gong ,  Chen Li ,  Qiang Liu ,  Ping Yan ,  Gang Chen ,  HaiTao Zhang ,  Ruizhen Cui

    发明人： Mali Gong ,  Chen Li ,  Qiang Liu ,  Ping Yan ,  Gang Chen ,  HaiTao Zhang ,  Ruizhen Cui

    IPC分类号： H01S3/042 ,  H01S3/06 ,  H01S3/09 ,  H01S3/0941 ,  H01S3/16

    CPC分类号： H01S3/0606 ,  H01S3/042 ,  H01S3/0615 ,  H01S3/08095 ,  H01S3/094057 ,  H01S3/0941 ,  H01S3/1611 ,  H01S3/1643 ,  H01S3/2316

    摘要： A corner-pumping method for slab laser and a solid-state laser gain module are disclosed. In one embodiment, said method comprises directing a pump light into a laser slab through slab corners of said laser slab; propagating the pump light within the laser slab by total internal reflection (TIR); and substantially absorbing the pump light during propagating. In another embodiment, said gain module comprises a laser slab formed by a solid state laser material, said laser slab including slab corners with corner faces; and a pump source providing a pump light; wherein said pump light is directed into said laser slab through said slab corners of said laser slab, propagated within said laser slab by total internal reflection (TIR), and substantially absorbed during propagation; and wherein said laser slab outputs an amplified laser beam.

    摘要翻译： 公开了一种用于平板激光器和固态激光增益模块的角式抽取方法。 在一个实施例中，所述方法包括将泵浦光通过所述激光板的板角引导到激光板中; 通过全内反射（TIR）传播激光板内的泵浦光; 并且在传播期间基本上吸收泵浦光。 在另一个实施例中，所述增益模块包括由固体激光材料形成的激光平板，所述激光板包括具有角面的平板角; 以及提供泵浦光的泵浦源; 其中所述泵浦光通过所述激光板的所述板角转入所述激光板，通过全内反射（TIR）在所述激光板内传播，并且在传播期间基本上被吸收; 并且其中所述激光板输出放大的激光束。

    Pyrophosphorolysis activated polymerization (PAP)
    30.
    发明申请
    Pyrophosphorolysis activated polymerization (PAP) 有权
    焦磷酸活化聚合（PAP）

    公开(公告)号：US20050095608A1

    公开(公告)日：2005-05-05

    申请号：US10798844

    申请日：2004-03-12

    申请人： Qiang Liu ,  Steve Sommer ,  Arthur Riggs

    发明人： Qiang Liu ,  Steve Sommer ,  Arthur Riggs

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6827 ,  C12Q1/6858 ,  C12Q2525/186 ,  C12Q2521/319 ,  C12Q2535/125 ,  C12Q2565/301 ,  C12Q2535/131

    摘要： A novel method of pyrophosphorolysis activated polymerization (PAP) has been developed. In PAP, pyrophosphorolysis and polymerization by DNA polymerase are coupled serially for each amplification by using an activatable oligonucleotide P* that has a non-extendible 3′-deoxynucleotide at its 3′ terminus. PAP can be applied for exponential amplification or for linear amplification. PAP can be applied to amplification of a rare allele in admixture with one or more wild-type alleles by using an activatable oligonucleotide P* that is an exact match at its 3′ end for the rare allele but has a mismatch at or near its 3′ terminus for the wild-type allele. PAP is inhibited by a mismatch in the 3′ specific sequence as far as 16 nucleotides away from the 3′ terminus. PAP can greatly increase the specificity of detection of an extremely rare mutant allele in the presence of the wild-type allele. Specificity results from both pyrophosphorolysis and polymerization since significant nonspecific amplification requires the combination of mismatch pyrophosphorolysis and misincorporation by the DNA polymerase, an extremely rare event. Using genetically engineered DNA polymerases greatly improves the efficiency of PAP.

    摘要翻译： 开发了焦磷酸活化聚合（PAP）的新方法。 在PAP中，通过使用在其3'末端具有不可延长的3'-脱氧核苷酸的可活化寡核苷酸P *，通过DNA聚合酶进行焦磷酸分解和聚合反应，进行每次扩增。 PAP可以用于指数放大或线性放大。 PAP可以应用于稀释等位基因与一种或多种野生型等位基因的混合，通过使用可激活的寡核苷酸P *，其在3'末端为罕见等位基因而精确匹配，但在3或3附近具有不匹配 '末端为野生型等位基因。 PAP被3'特异性序列的错配抑制至远离3'末端的16个核苷酸。 PAP可以在野生型等位基因存在的情况下大大增加检测极罕见的突变等位基因的特异性。 焦磷酸分解和聚合的特异性结果，因为显着的非特异性扩增需要由DNA聚合酶引起的错配焦磷酸分解和错配合的组合，这是非常罕见的事件。 使用基因工程DNA聚合酶大大提高了PAP的效率。