Magnetic head with medium sliding surface having varied curvature and recording/reproducing apparatus
    21.
    发明授权
    Magnetic head with medium sliding surface having varied curvature and recording/reproducing apparatus 失效
    具有中等滑动面的磁头具有不同的曲率和记录/重放装置

    公开(公告)号:US06989961B2

    公开(公告)日:2006-01-24

    申请号:US10601169

    申请日:2003-06-20

    IPC分类号: G11B5/187

    CPC分类号: G11B5/1878 G11B5/0086

    摘要: A magnetic head includes core blocks having a medium sliding surface formed on one surface of the core blocks, the medium sliding surface having a slender convex curved shape formed along a sliding direction of a recording medium from the upstream side of the sliding direction to the downstream side, and the medium sliding surface having a magnetic gap formed thereon, wherein the medium sliding surface is shaped along a longitudinal direction thereof like a circular arc with a radius of curvature R while being shaped along a width direction like a circular arc with a radius of curvature r, which is smaller than the radius of curvature R, so that the radius of curvature r is continuously reduced with closer distance to the downstream end of the recording medium sliding direction from a vicinity of the magnetic gap, so that by employing the above magnetic head, a long life magnetic head with small damage due to foreign material adhesion as well as small abrasion can be provided.

    摘要翻译: 磁头包括具有形成在芯块的一个表面上的介质滑动表面的芯块,介质滑动表面具有从滑动方向的上游侧到下游的沿着记录介质的滑动方向形成的细长的凸形弯曲形状 并且介质滑动面具有形成在其上的磁隙,其中介质滑动表面沿着其纵向方向成像为具有曲率半径R的圆弧,同时沿着具有半径的圆弧的宽度方向成形 的曲率半径r比曲率半径R短,从而使距离磁记录介质滑动方向的下游端更靠近距离的曲率半径r连续地减小,从而通过采用 磁头以上,可以提供由于异物附着而造成的损伤小且磨损小的长寿命磁头。

    Method for producing L-amino acids
    22.
    发明授权
    Method for producing L-amino acids 失效
    生产L-氨基酸的方法

    公开(公告)号:US4980285A

    公开(公告)日:1990-12-25

    申请号:US412562

    申请日:1989-09-25

    摘要: A method for producing an L-amino acid, which comprises inserting a gene which codes for an enzyme which is utilized on the route of biosynthesis of an L-amino acid product into one of at least two plasmid vectors which have compatible replicating origins different from each other, inserting a second gene which codes for an enzyme different from the first enzyme on the route of biosynthesis of the L-amino acid into a second of the plasmid vectors; introducing the thus obtained recombinant plasmids into a strain of Coryneform bacteria; and culturing the thus transformed strain which is capable of producing the L-amino acid, said two enzymes being highly rate determining enzymes for the biosynthesis of L-amino acid.

    摘要翻译: 一种L-氨基酸的制造方法,其特征在于,将在L-氨基酸产物的生物合成途径中使用的酶编码的基因插入至少两个具有不同于其的复制起点的质粒载体之一 将在L-氨基酸生物合成途径上编码不同于第一酶的酶的第二基因插入第二质粒载体; 将如此获得的重组质粒导入棒状细菌菌株; 并培养能够产生L-氨基酸的如此转化的菌株,所述两种酶是用于生物合成L-氨基酸的高速率决定酶。

    Intracellular metabolic flux analysis method using substrate labeled with isotope
    23.
    发明授权
    Intracellular metabolic flux analysis method using substrate labeled with isotope 失效
    使用同位素标记的底物的细胞内代谢通量分析方法

    公开(公告)号:US08510054B2

    公开(公告)日:2013-08-13

    申请号:US11048923

    申请日:2005-02-03

    IPC分类号: G01N33/48 C12Q1/00

    摘要: A method for intracellular metabolic flux analysis comprising (a) culturing cells in a medium not containing any isotope-labeled substrate to a target phase of the metabolic flux analysis, (b) adding an isotope-labeled substrate to the medium, and continuing culture and collecting samples from the medium in time course, (c) measuring isotope distribution in an intracellular metabolite contained in the samples collected in time course, (d) performing a regression analysis for measured data and calculating an isotope distribution ratio in a steady state, and (e) analyzing a metabolic flux of the cultured cells by using the calculated isotope distribution ratio.

    摘要翻译: 一种用于细胞内代谢通量分析的方法,包括(a)在不含任何同位素标记的底物的培养基中将细胞培养至代谢通量分析的目标阶段,(b)将同位素标记的底物加入到培养基中, 在时间过程中从培养基中收集样品,(c)测量在时间过程中收集的样品中所含的细胞内代谢物中的同位素分布,(d)对稳定状态进行测量数据和计算同位素分布比的回归分析,以及 (e)使用计算出的同位素分布比分析培养细胞的代谢通量。

    Method for producing L-glutamic acid by fermentation accompanied by precipitation
    27.
    再颁专利
    Method for producing L-glutamic acid by fermentation accompanied by precipitation 有权
    通过发酵伴随沉淀生产L-谷氨酸的方法

    公开(公告)号:USRE42350E1

    公开(公告)日:2011-05-10

    申请号:US12429421

    申请日:2009-04-24

    摘要: A microorganism is provided which can metabolize a carbon source at a specific pH in a liquid medium containing L-glutamic acid at a saturation concentration and the carbon source, and which has ability to accumulate L-glutamic acid in an amount exceeding the amount corresponding to the saturation concentration in the liquid medium at the pH. Also provided is a method for producing L-glutamic acid by fermentation, which comprises culturing the microorganism in a liquid medium of which pH is adjusted to a pH at which L-glutamic acid is precipitated, to produce and accumulate L-glutamic acid and precipitate L-glutamic acid in the medium.

    摘要翻译: 提供了能够在饱和浓度和碳源的含有L-谷氨酸的液体培养基中在特定pH下代谢碳源的微生物,并且具有将L-谷氨酸的积累量超过对应于 在液体介质中的饱和浓度在pH值。 还提供了通过发酵生产L-谷氨酸的方法,其包括在将pH调节至L-谷氨酸沉淀的pH的液体培养基中培养微生物以产生并积累L-谷氨酸并沉淀 培养基中的L-谷氨酸。

    METHOD FOR PRODUCTION OF L-AMINO ACID
    29.
    发明申请
    METHOD FOR PRODUCTION OF L-AMINO ACID 有权
    L-氨基酸生产方法

    公开(公告)号:US20090239269A1

    公开(公告)日:2009-09-24

    申请号:US12420934

    申请日:2009-04-09

    IPC分类号: C12P13/20 C12P13/08

    CPC分类号: C12N9/88 C12P13/08 C12P13/227

    摘要: A bacterium which belongs to the Enterobacteriaceae family and has an ability to produce an L-amino acid such as L-lysine, L-threonine and L-tryptophan and is modified to enhance glutamic acid decarboxylase activity is cultured in a medium to produce and accumulate the L-amino acid in the medium or cells of the bacterium. Then, the L-amino acid is collected from the medium or the cells.

    摘要翻译: 属于肠杆菌科的具有生产L-赖氨酸,L-苏氨酸和L-色氨酸的L-氨基酸的能力并经过修饰以增强谷氨酸脱羧酶活性的细菌在培养基中培养以产生和积累 细菌的培养基或细胞中的L-氨基酸。 然后,从培养基或细胞收集L-氨基酸。

    METHOD FOR PRODUCING L-AMINO ACID
    30.
    发明申请
    METHOD FOR PRODUCING L-AMINO ACID 审中-公开
    生产L-氨基酸的方法

    公开(公告)号:US20090093029A1

    公开(公告)日:2009-04-09

    申请号:US12202484

    申请日:2008-09-02

    IPC分类号: C12P13/04 C12P13/08 C12P13/22

    摘要: An L-amino acid is produced by culturing an Enterobacteriaceae which is able to produce an L-amino acid in a medium containing glycerol, especially crude glycerol, as the carbon source to produce and accumulate the L-amino acid in the culture, and collecting the L-amino acid from the culture.

    摘要翻译: 通过培养能够在含有甘油,特别是粗甘油的培养基中产生L-氨基酸的肠杆菌科生产L-氨基酸作为碳源,以在培养物中产生和积累L-氨基酸,并收集 来自培养物的L-氨基酸。