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公开(公告)号：US07670822B2
公开(公告)日：2010-03-02
申请号：US12212767
申请日：2008-09-18
摘要： A novel aldolase is described. 4-hydroxy-3-methyl-2-keto-pentanoic acid, which is useful as an intermediate in the synthesis of 4-hydroxy-L-isoleucine, may be synthesized from acetaldehyde and α-ketobutyric acid using a novel aldolase, which is derived from the genus Arthrobacter.
摘要翻译： 描述了一种新的醛缩酶。 可用于合成4-羟基-L-异亮氨酸的中间体的4-羟基-3-甲基-2-酮戊酸可以使用新的醛缩酶由乙醛和α-酮丁酸合成，其是 衍生自节杆菌属。
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公开(公告)号：US20090104659A1
公开(公告)日：2009-04-23
申请号：US12212767
申请日：2008-09-18
摘要： A novel aldolase is described. 4-hydroxy-3-methyl-2-keto-pentanoic acid, which is useful as an intermediate in the synthesis of 4-hydroxy-L-isoleucine, may be synthesized from acetaldehyde and α-ketobutyric acid using a novel aldolase, which is derived from the genus Arthrobacter.
摘要翻译： 描述了一种新的醛缩酶。 可用作合成4-羟基-L-异亮氨酸的中间体的4-羟基-3-甲基-2-酮 - 戊酸可以使用新的醛缩酶，由乙醛和α-酮丁酸合成，其是 衍生自节杆菌属。
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3.发明授权公开(公告)号：US08129151B2
公开(公告)日：2012-03-06
申请号：US11624080
申请日：2007-01-17
申请人： Mika Moriya , Hiroshi Izui , Eiji Ono , Kazuhiko Matsui , Hisao Ito , Yoshihiko Hara
发明人： Mika Moriya , Hiroshi Izui , Eiji Ono , Kazuhiko Matsui , Hisao Ito , Yoshihiko Hara
CPC分类号： C12N9/0016 , C12N9/88 , C12P13/14 , C12R1/01 , C12R1/425 , Y10S435/822 , Y10S435/88
摘要： L-Glutamic acid is produced by culturing in a liquid culture medium a microorganism belonging to the genus Pantoea or Serratia and having an ability to produce L-glutamic acid, which increases in an activity of enzyme catalyzing a reaction for L-glutamic acid biosynthesis or which decreases in or is deficient in an activity of an enzyme catalyzing a reaction branching from a pathway for L-glutamic acid biosynthesis and producing a compound other than L-glutamic acid, and collecting produced L-glutamic acid from the culture medium.
摘要翻译： L-谷氨酸通过在液体培养基中培养属于泛酸属或沙雷氏菌属的具有产生L-谷氨酸能力的微生物产生，其增加了催化L-谷氨酸生物合成反应的酶的活性，或 其催化从L-谷氨酸生物合成途径分支的反应的酶的活性降低或缺乏，并产生除L-谷氨酸以外的化合物，并从培养基中收集L-谷氨酸。
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4.发明授权公开(公告)号：US07833761B2
公开(公告)日：2010-11-16
申请号：US12202476
申请日：2008-09-02
CPC分类号： C12N9/0008 , C12P13/06 , C12P13/08 , C12P13/222 , C12P13/227
摘要： A microorganism is provided which has an ability to produce an L-amino acid such as L-lysine, L-tryptophan, L-phenylalanine, L-valine, L-leucine, L-isoleucine and L-serine, and has been modified to increase the activity of pyruvate synthase or pyruvate:NADP+ oxidoreductase. This microorganism is cultured in a medium containing ethanol or an aliphatic acid as the carbon source to produce and accumulate the L-amino acid in the medium or cells, and the L-amino acid is collected from the medium or the cells.
摘要翻译： 提供了具有产生L-赖氨酸，L-色氨酸，L-苯丙氨酸，L-缬氨酸，L-亮氨酸，L-异亮氨酸，L-丝氨酸等L-氨基酸的能力的微生物， 增加丙酮酸合酶或丙酮酸：NADP +氧化还原酶的活性。 将该微生物在含有乙醇或脂肪酸作为碳源的培养基中培养，以在培养基或细胞中产生并积累L-氨基酸，从培养基或细胞收集L-氨基酸。
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公开(公告)号：US20100081180A1
公开(公告)日：2010-04-01
申请号：US12579594
申请日：2009-10-15
CPC分类号： C12P7/46
摘要： An organic acid is produced by allowing a bacterium which has an ability to produce an organic acid and has been modified so that expression of yidE gene is enhanced, or a product obtained by processing the bacterium, to act on an organic raw material in a reaction mixture containing carbonate ions, bicarbonate ions, or carbon dioxide gas to produce the organic acid, and collecting the organic acid.
摘要翻译： 有机酸是通过使具有生产有机酸的能力的细菌进行改性的，以使yidE基因的表达增强，或通过加工细菌获得的产物作用于反应中的有机原料 含有碳酸根离子，碳酸氢根离子或二氧化碳气体的混合物以产生有机酸，并收集有机酸。
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6.发明授权Method of constructing amino acid producing bacterial strains, and method of preparing amino acids by fermentation with the constructed amino acid producing bacterial strains 有权构建产氨酸的细菌菌株的方法，以及用构建的产生氨基酸的菌株通过发酵制备氨基酸的方法公开(公告)号：US07608437B2
公开(公告)日：2009-10-27
申请号：US11218433
申请日：2005-09-06
申请人： Yoko Asakura , Jun Nakamura , Sohei Kanno , Mikiko Suga , Eiichiro Kimura , Hisao Ito , Kazuhiko Matsui , Tsuyoshi Ohsumi , Tsuyoshi Nakamatsu , Osamu Kurahashi
发明人： Yoko Asakura , Jun Nakamura , Sohei Kanno , Mikiko Suga , Eiichiro Kimura , Hisao Ito , Kazuhiko Matsui , Tsuyoshi Ohsumi , Tsuyoshi Nakamatsu , Osamu Kurahashi
IPC分类号： C12P13/14 , C12Q1/48 , C12Q1/32 , C12Q1/68 , C12N9/02 , C12N9/10 , C12N1/21 , C12P21/00 , C12N15/00 , C07K14/00 , C07H21/00
摘要： A method of producing coryneform bacteria having an improved amino acid or nucleic acid-productivity comprises the steps of introducing a mutation in a promoter sequence of amino acid- or nucleic acid-biosynthesizing genes on a chromosome of a coryneform bacterium so that it is close to a consensus sequence or introducing a change in the promoter sequence of amino acid- or nucleic acid-biosynthesizing genes on the chromosome of a coryneform bacterium by gene recombination so that it is close to a consensus sequence, obtaining mutants of the coryneform amino acid- or nucleic acid-producing microorganism, culturing the mutants and select a mutant capable of producing the intended amino acid or nucleic acid in a large amount. This method allows one of skill in the art to construct a mutant capable of enriching or controlling the expression of an intended gene without using a plasmid and to promote production of amino acids in a high yield, by the recombination or mutation.
摘要翻译： 具有改进的氨基酸或核酸生产力的棒状杆菌细菌的方法包括以下步骤：将氨基酸或核酸 - 生物合成基因的启动子序列中的突变引入棒状细菌的染色体上，使其接近 共有序列或通过基因重组在棒状细菌的染色体上引入氨基酸或核酸 - 生物合成基因的启动子序列的变化，使得其接近共有序列，获得棒状杆菌型氨基酸的突变体或 产生核酸的微生物，培养突变体并选择能够大量产生所需氨基酸或核酸的突变体。 该方法允许本领域技术人员通过重组或突变来构建能够富集或控制预期基因的表达而不使用质粒并以高产率促进氨基酸产生的突变体。
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7.发明申请L-AMINO ACID-PRODUCING BACTERIUM AND A METHOD FOR PRODUCING AN L-AMINO ACID 有权L-氨基酸生产细菌和一种生产L-氨基酸的方法公开(公告)号：US20090246835A1
公开(公告)日：2009-10-01
申请号：US12055438
申请日：2008-03-26
CPC分类号： C07K14/245 , C12P13/04 , C12P13/08 , C12P13/22
摘要： An L-amino acid is produced by culturing a microorganism of the family Enterobacteriaceae which has the ability to produce an L-amino acid and which has been modified so as to increase the expression of the evgA gene, the gadE gene, and/or the ydeO gene. These genes encode a transcription factor involved in the EvgAS two-component system regulon. The culture takes place in a medium, and the L-amino acid is collected from the medium or cells.
摘要翻译： L-氨基酸是通过培养具有产生L-氨基酸的能力的肠杆菌科的微生物而产生的，该微生物被修饰以增加evgA基因，gadE基因和/或 ydeO基因。 这些基因编码参与EvgAS双组分系统调节子的转录因子。 培养物在培养基中进行，从培养基或细胞收集L-氨基酸。
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8.发明申请公开(公告)号：US20060234357A1
公开(公告)日：2006-10-19
申请号：US11422969
申请日：2006-06-08
CPC分类号： C12N9/0016 , C07H21/04 , C07K14/34 , C12N9/1007 , C12N9/22 , C12P13/04 , C12P21/00 , C12P21/02
摘要： The present invention provides novel polypeptides and polynucleotides useful as biotechnological tools, specifically identified in a coryneform bacterium Corynebacterium glutamicum ssp. lactofermentum and methods of producing substances in organisms having enhanced or attenuated expression of these polypeptides and/or polynucleotides.
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公开(公告)号：US07090998B2
公开(公告)日：2006-08-15
申请号：US10613990
申请日：2003-07-08
CPC分类号： C07K14/245
摘要： In a method for producing a target substance utilizing a microorganism, which comprises culturing a γ-proteobacterium in a medium to produce and accumulate the target substance in the medium or cells and collecting the target substance, there is used a strain in which the ArcA protein does not normally function in the cell by means of, for example, disruption of the arcA gene on the chromosome.
摘要翻译： 在利用微生物生产目标物质的方法中，其包括在培养基中培养γ-谷氨酸杆菌，以在培养基或细胞中产生并积累目标物质并收集目标物质，使用其中的ArcA蛋白 通常不会通过例如染色体上arcA基因的破坏在细胞中起作用。
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10.发明授权Method for producing L-glutamic acid by fermentation accompanied by precipitation 有权通过发酵伴随沉淀生产L-谷氨酸的方法公开(公告)号：US07015010B1
公开(公告)日：2006-03-21
申请号：US09641892
申请日：2000-08-18
申请人： Hiroshi Izui , Mika Moriya , Seiko Hirano , Yoshihiko Hara , Hisao Ito , Kazuhiko Matsui
发明人： Hiroshi Izui , Mika Moriya , Seiko Hirano , Yoshihiko Hara , Hisao Ito , Kazuhiko Matsui
IPC分类号： C12Q1/04
CPC分类号： C12N9/0016 , C12N9/88 , C12P13/14 , C12R1/01
摘要： A microorganism which can metabolize a carbon source at a specific pH in a liquid medium containing L-glutamic acid at a saturation concentration and the carbon source, and has ability to accumulate L-glutamic acid in an amount exceeding the amount corresponding to the saturation concentration in the liquid medium at the pH; and a method for producing L-glutamic acid by fermentation, which comprises culturing the microorganism in a liquid medium of which pH is adjusted to a pH at which L-glutamic acid is precipitated, to produce and accumulate L-glutamic acid and precipitate L-glutamic acid in the medium.
摘要翻译： 在含有饱和浓度的L-谷氨酸和碳源的液体培养基中，能够在特定pH下代谢碳源的微生物，并且具有以超过相应于饱和浓度的量累积L-谷氨酸的能力 在液体介质中的pH值; 以及通过发酵生产L-谷氨酸的方法，其包括在将pH调节至L-谷氨酸沉淀的pH的液体培养基中培养微生物以产生并积累L-谷氨酸并将L-谷氨酸沉淀， 培养基中的谷氨酸。
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