公开(公告)号：US20150218604A1

公开(公告)日：2015-08-06

申请号：US14626505

申请日：2015-02-19

Applicant: Solazyme, Inc.

Inventor： Scott Franklin ,  Aravind Somanchi ,  Karen Espina ,  George Rudenko ,  Penelope Chua

IPC: C12P7/64 ,  C12N9/24 ,  C12N9/16

CPC classification number: C12P7/6427 ,  C07C1/2078 ,  C07K2319/01 ,  C10L1/02 ,  C12N9/00 ,  C12N9/16 ,  C12N9/2402 ,  C12N15/79 ,  C12P7/20 ,  C12P7/64 ,  C12P7/6409 ,  C12P7/6418 ,  C12P7/6463 ,  C12P7/649 ,  C12Y301/02014 ,  C12Y302/01026 ,  Y02E50/13 ,  Y02P20/52 ,  Y02P60/877 ,  Y02T50/678

Abstract: Disclosed herein are methods of manufacturing renewable chemicals through the manufacture of novel triglyceride oils followed by chemical modification of the oils. Methods such as transesterification, hydrogenation, hydrocracking, deoxygenation, isomerization, interesterification, hydroxylation, hydrolysis and saponification are disclosed. Novel oils containing fatty acid chain lengths of C8, C10, C12 or C14 are also disclosed and are useful as feedstocks in the methods of the invention.

Abstract translation: 本文公开了通过制备新型甘油三酯油然后化学改性油来制造可再生化学品的方法。 公开了酯交换，氢化，加氢裂化，脱氧，异构化，酯交换，羟基化，水解和皂化等方法。 还公开了含有C8，C10，C12或C14脂肪酸链长度的新型油，并且可用作本发明方法中的原料。

公开(公告)号：US08951777B2

公开(公告)日：2015-02-10

申请号：US13852116

申请日：2013-03-28

Applicant: Solazyme, Inc.

Inventor： Scott Franklin ,  Aravind Somanchi ,  Karen Espina ,  George Rudenko ,  Penelope Chua

IPC: C12N1/12 ,  C12N15/82 ,  C12N9/24 ,  C12P7/64 ,  C10L1/02 ,  C12N9/00 ,  C12N9/16 ,  C12P7/20 ,  C12N15/79 ,  C07C1/207

CPC classification number: C12P7/6427 ,  C07C1/2078 ,  C07K2319/01 ,  C10L1/02 ,  C12N9/00 ,  C12N9/16 ,  C12N9/2402 ,  C12N15/79 ,  C12P7/20 ,  C12P7/64 ,  C12P7/6409 ,  C12P7/6418 ,  C12P7/6463 ,  C12P7/649 ,  C12Y301/02014 ,  C12Y302/01026 ,  Y02E50/13 ,  Y02P20/52 ,  Y02P60/877 ,  Y02T50/678

Abstract: Disclosed herein are obligate heterotrophic microalgae cells containing an exogenous gene. In some embodiments the gene is a sucrose utilization gene, and further disclosed are methods of manufacturing triglyceride oils using sugar cane or sugar beets as a feedstock in a heterotrophic fermentation. In other embodiments the feedstock is depolymerized cellulosic material. Also disclosed are cells that produce medium chain fatty acids at levels not produced in non-recombinant cells of the same species and genus.

Abstract translation: 本文公开了含有外源基因的专性异养微藻细胞。 在一些实施方案中，该基因是蔗糖利用基因，并且进一步公开了使用甘蔗或甜菜作为异养发酵中的原料来制备甘油三酯油的方法。 在其它实施方案中，原料是解聚的纤维素材料。 还公开了以相同物种和属的非重组细胞中不产生的水平产生中链脂肪酸的细胞。

公开(公告)号：US08633012B2

公开(公告)日：2014-01-21

申请号：US13650024

申请日：2012-10-11

Applicant: Solazyme, Inc.

Inventor： Scott Franklin ,  Aravind Somanchi ,  Janice Wee ,  George Rudenko ,  Jeffrey L. Moseley ,  Walt Rakitsky ,  Xinhua Zhao ,  Riyaz Bhat

IPC: C12N1/13 ,  C12N1/12 ,  C12P7/64 ,  C12N15/56 ,  C12N9/18 ,  C07H21/04

CPC classification number: C12P7/6463 ,  C07C69/52 ,  C10L1/02 ,  C10L2200/0476 ,  C10L2290/26 ,  C10L2290/30 ,  C12N9/0071 ,  C12N9/1029 ,  C12N9/16 ,  C12N15/52 ,  Y02E50/13

Abstract: Methods and compositions for the production of oil, fuels, oleochemicals, and other compounds in recombinant microorganisms are provided, including oil-bearing microorganisms and methods of low cost cultivation of such microorganisms. Microalgal cells containing exogenous genes encoding, for example, a lipase, a sucrose transporter, a sucrose invertase, a fructokinase, a polysaccharide-degrading enzyme, a keto acyl-ACP synthase enzyme, a fatty acyl-ACP thioesterase, a fatty acyl-CoA/aldehyde reductase, a fatty acyl-CoA reductase, a fatty aldehyde reductase, a fatty acid hydroxylase, a desaturase enzyme, a fatty aldehyde decarbonylase, and/or an acyl carrier protein are useful in manufacturing transportation fuels such as renewable diesel, biodiesel, and renewable jet fuel, as well as oleochemicals such as functional fluids, surfactants, soaps and lubricants.

Abstract translation: 提供了用于在重组微生物中生产油，燃料，油化合物和其它化合物的方法和组合物，包括含油微生物和这种微生物的低成本培养的方法。 包含编码例如脂肪酶，蔗糖转运蛋白，蔗糖转化酶，果糖激酶，多糖降解酶，酮酰基-ACP合成酶，脂肪酰基-ACP硫酯酶，脂肪酰辅酶A的外源基因的微藻细胞 /醛还原酶，脂肪酰辅酶A还原酶，脂肪醛还原酶，脂肪酸羟化酶，去饱和酶，脂肪醛脱羰酶和/或酰基载体蛋白，可用于制造运输燃料如可再生柴油，生物柴油， 和可再生喷气燃料，以及油化学品如功能流体，表面活性剂，肥皂和润滑剂。

公开(公告)号：US20130317240A1

公开(公告)日：2013-11-28

申请号：US13941357

申请日：2013-07-12

Applicant: Solazyme, Inc.

Inventor： Scott Franklin ,  Aravind Somanchi ,  George Rudenko ,  Riyaz Bhat ,  Xinhua Zhao ,  Risha Bond ,  Walter Rakitsky ,  Alejandro Marangoni ,  Diza Braksmayer

IPC: C12P7/64

CPC classification number: C12P7/6409 ,  A23D9/00 ,  A23D9/013 ,  C07C57/03 ,  C07J9/00 ,  C11B1/00 ,  C12N9/0071 ,  C12N9/1029 ,  C12N9/16 ,  C12N9/2402 ,  C12N15/113 ,  C12N15/8247 ,  C12N2310/531 ,  C12P7/64 ,  C12P7/6445 ,  C12P7/6463 ,  C12P7/6472 ,  C12P33/00 ,  C12R1/89 ,  C12Y203/01015 ,  C12Y203/0102 ,  C12Y203/01041 ,  C12Y203/01051 ,  C12Y203/01179 ,  C12Y203/0118 ,  C12Y302/01014 ,  C12Y302/01026 ,  Y02E50/13 ,  Y02P20/52

Abstract: Recombinant DNA techniques are used to produce oleaginous recombinant cells that produce triglyceride oils having desired fatty acid profiles and regiospecific or stereospecific profiles. Genes manipulated include those encoding stearoyl-ACP desturase, delta 12 fatty acid desaturase, acyl-ACP thioesterase, ketoacyl-ACP synthase, and lysophosphatidic acid acyltransferase. The oil produced can have enhanced oxidative or thermal stability, or can be useful as a frying oil, shortening, roll-in shortening, tempering fat, cocoa butter replacement, as a lubricant, or as a feedstock for various chemical processes. The fatty acid profile can be enriched in midchain profiles or the oil can be enriched in triglycerides of the saturated-unsaturated-saturated type.

公开(公告)号：US20130296591A1

公开(公告)日：2013-11-07

申请号：US13889221

申请日：2013-05-07

Applicant: Solazyme, Inc.

Inventor： Anthony G. Day ,  Geoffrey Brooks ,  Scott Franklin

IPC: C11B3/02

CPC classification number: C11B3/02 ,  A23D7/00 ,  A23D9/00 ,  C11D9/26 ,  C12P7/6418 ,  C12P7/6463 ,  C12P7/649 ,  Y02E50/13

Abstract: Chemically modified lipids and methods for their preparation are provided.

Abstract translation: 提供化学修饰的脂质及其制备方法。

公开(公告)号：US09434909B2

公开(公告)日：2016-09-06

申请号：US14184288

申请日：2014-02-19

Applicant: Solazyme, Inc.

Inventor： Donald E. Trimbur ,  Chung-Soon Im ,  Harrison F. Dillon ,  Anthony G. Day ,  Scott Franklin ,  Anna Coragliotti

IPC: A61K48/00 ,  C07H21/04 ,  C12N1/12 ,  C12N9/14 ,  C12P7/20 ,  C11C3/00 ,  C10L1/02 ,  C12N1/32 ,  C12N9/04 ,  C12N9/02 ,  C12N9/12 ,  C12N9/16 ,  C12N9/20 ,  C12N9/26 ,  C12N9/88 ,  C12P5/02 ,  C10L1/06 ,  C12P7/64

CPC classification number: C10L1/026 ,  C07H21/04 ,  C10L1/06 ,  C10L2200/0484 ,  C10L2270/026 ,  C10L2270/04 ,  C11C3/00 ,  C12N1/12 ,  C12N1/32 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/1205 ,  C12N9/14 ,  C12N9/16 ,  C12N9/20 ,  C12N9/2408 ,  C12N9/2431 ,  C12N9/88 ,  C12N15/79 ,  C12P5/02 ,  C12P7/6409 ,  C12P7/6436 ,  C12P7/6445 ,  C12P7/6463 ,  C12P7/649 ,  C12P21/06 ,  C12Y302/01026 ,  Y02E50/10 ,  Y02E50/13 ,  Y02E50/343 ,  Y02P20/52 ,  Y02T50/678

Abstract: The invention provides methods of manufacturing alkanes from triglyceride oils produced through fermentation of oil-bearing microbes. The processes provided herein can utilize a variety of carbohydrate feedstocks including cane bagasse, sugar beet pulp, corn stover, glycerol, corn starch, sorghum, molasses, waste glycerol, and other renewable materials. These processes further comprise hydrotreating, hydrocracking, isomerization, distillation, and other petrochemical processes for use with oil-bearing microbes and products derived therefrom to manufacture fuels. Particular embodiments include the manufacture of ASTM D975 and ASTM D1655 compliant fuels. Genetically engineered microbes provided herein can be used in the manufacture of renewable diesel and renewable jet fuel.

Abstract translation: 本发明提供了通过含油微生物发酵产生的甘油三酯油制备烷烃的方法。 本文提供的方法可以利用多种碳水化合物原料，包括甘蔗渣，甜菜浆，玉米秸秆，甘油，玉米淀粉，高粱，糖蜜，废甘油和其他可再生材料。 这些方法还包括与含油微生物一起使用的加氢处理，加氢裂化，异构化，蒸馏和其它石油化学方法以及由其衍生的产物来制造燃料。 具体实施方案包括制造ASTM D975和符合ASTM D1655的燃料。 本文提供的基因工程微生物可用于制造可再生柴油和可再生喷气燃料。

公开(公告)号：US20160186191A1

公开(公告)日：2016-06-30

申请号：US14975016

申请日：2015-12-18

Applicant: Solazyme, Inc.

Inventor： Scott Franklin ,  Aravind Somanchi ,  Janice Wee ,  George Rudenko ,  Jeffrey L. Moseley ,  Walter Rakitsky

IPC: C12N15/79 ,  C12N9/02 ,  C12N9/26 ,  C12N9/40 ,  C12P7/64 ,  C12N9/16

CPC classification number: C12N15/79 ,  A23D7/00 ,  A23D9/00 ,  A23K20/158 ,  C11B1/00 ,  C11C3/00 ,  C12N9/0071 ,  C12N9/16 ,  C12N9/2431 ,  C12N9/2465 ,  C12N15/67 ,  C12N15/8216 ,  C12P7/64 ,  C12P7/6445 ,  C12P7/6463 ,  C12Y114/19001 ,  C12Y301/02 ,  C12Y301/02014 ,  C12Y302/01022 ,  C12Y302/01026 ,  Y02E50/13 ,  Y02P30/20 ,  Y02T50/678

Abstract: Methods and compositions for the production of oil, fuels, oleochemicals, and other compounds in recombinant microorganisms are provided, including oil-bearing microorganisms and methods of low cost cultivation of such microorganisms. Microalgal cells containing exogenous genes encoding, for example, a lipase, a sucrose transporter, a sucrose invertase, a fructokinase, a polysaccharide-degrading enzyme, a keto acyl-ACP synthase enzyme, a fatty acyl-ACP thioesterase, a fatty acyl-CoA/aldehyde reductase, a fatty acyl-CoA reductase, a fatty aldehyde reductase, a fatty aldehyde decarbonylase, and/or an acyl carrier protein are useful in manufacturing transportation fuels such as renewable diesel, biodiesel, and renewable jet fuel, as well as oleochemicals such as functional fluids, surfactants, soaps and lubricants.

Abstract translation: 提供了用于在重组微生物中生产油，燃料，油化合物和其它化合物的方法和组合物，包括含油微生物和这种微生物的低成本培养的方法。 包含编码例如脂肪酶，蔗糖转运蛋白，蔗糖转化酶，果糖激酶，多糖降解酶，酮酰基-ACP合成酶，脂肪酰基-ACP硫酯酶，脂肪酰辅酶A的外源基因的微藻细胞 /醛还原酶，脂肪酰辅酶A还原酶，脂肪醛还原酶，脂肪醛脱羰酶和/或酰基载体蛋白质可用于制造运输燃料如可再生柴油，生物柴油和可再生喷气燃料，以及油脂化学品 例如功能流体，表面活性剂，肥皂和润滑剂。

公开(公告)号：US20150344917A1

公开(公告)日：2015-12-03

申请号：US14730671

申请日：2015-06-04

Applicant: Solazyme, Inc.

Inventor： Scott Franklin ,  Walter Rakitsky ,  George Rudenko ,  Xinhua Zhao ,  Felipe Arana Rodriguez ,  Wenhua Lu ,  Janice Wee

IPC: C12P7/64 ,  A23D9/02 ,  C11B3/00 ,  H01B3/20 ,  C10M169/04

CPC classification number: C12P7/6463 ,  A23D9/007 ,  A23D9/02 ,  C10M169/041 ,  C11B1/025 ,  C11B1/06 ,  C11B1/10 ,  C11B3/001 ,  C11B3/006 ,  C11B3/008 ,  C11B3/06 ,  C11B3/10 ,  C11B3/14 ,  C12N9/2442 ,  C12N15/79 ,  C12P7/6409 ,  C12Y301/02014 ,  H01B3/20 ,  H01F27/105 ,  H01F27/321

Abstract: Methods and compositions for the production of dielectric fluids from lipids produced by microorganisms are provided, including oil-bearing microorganisms and methods of low cost cultivation of such microorganisms. Microalgal cells containing exogenous genes encoding, for example, a sucrose transporter, a sucrose invertase, a fructokinase, a polysaccharide-degrading enzyme, a lipid pathway modification enzyme, a fatty acyl-ACP thioesterase, a desaturase, a fatty acyl-CoA/aldehyde reductase, and/or an acyl carrier protein are useful in manufacturing dielectric fluids.

Abstract translation: 提供了由微生物产生的脂质生产介电流体的方法和组合物，包括含油微生物和这种微生物的低成本培养方法。 含有编码例如蔗糖转运蛋白，蔗糖转化酶，果糖激酶，多糖分解酶，脂质途径修饰酶，脂肪酰基-ACP硫酯酶，去饱和酶，脂肪酰辅酶A /醛的外源基因的微藻细胞 还原酶和/或酰基载体蛋白质可用于制造介电流体。

公开(公告)号：US20150125914A1

公开(公告)日：2015-05-07

申请号：US14506491

申请日：2014-10-03

Applicant: SOLAZYME, INC.

Inventor： Scott Franklin ,  Aravind Somanchi ,  George Rudenko ,  Riyaz Bhat ,  Xinhua Zhao ,  Jeffrey L. Moseley

IPC: C12P7/64 ,  C11C1/00 ,  C11D9/00

CPC classification number: C12P7/64 ,  C11C1/002 ,  C11D9/00 ,  C12P7/6409 ,  C12Y203/01041 ,  C12Y301/02014

Abstract: Recombinant DNA techniques are used to produce oleaginous recombinant cells that produce triglyceride oils having desired fatty acid profiles and regiospecific or stereospecific profiles. Genes manipulated include those encoding stearoyl-ACP desaturase, delta 12 fatty acid desaturase, acyl-ACP thioesterase, ketoacyl-ACP synthase, and lysophosphatidic acid acyltransferase. The oil produced can have enhanced oxidative or thermal stability, or can be useful as a frying oil, shortening, roll-in shortening, tempering fat, cocoa butter replacement, as a lubricant, or as a feedstock for various chemical processes. The fatty acid profile can be enriched in midchain profiles or the oil can be enriched in triglycerides of the saturated-unsaturated-saturated type.

Abstract translation: 重组DNA技术用于产生产生具有所需脂肪酸谱和区域特异性或立体特异性谱的甘油三酯油的含油重组细胞。 基因操作包括编码硬脂酰-ACP去饱和酶，δ12脂肪酸去饱和酶，酰基-ACP硫酯酶，酮酰基-ACP合成酶和溶血磷脂酰基转移酶的那些。 所生产的油可以具有增强的氧化或热稳定性，或可用作煎炸油，起酥油，卷入起酥油，回火脂肪，可可脂替代物，润滑剂或用作各种化学方法的原料。 脂肪酸分布可以富集中链型，或者油可富含饱和不饱和饱和型甘油三酸酯。

公开(公告)号：US20140288320A1

公开(公告)日：2014-09-25

申请号：US14209931

申请日：2014-03-13

Applicant: Solazyme, Inc.

Inventor： George N. Rudenko ,  Jason Casolari ,  Scott Franklin

IPC: C12P7/64 ,  C12N9/16

CPC classification number: C12P7/6409 ,  C12N1/12 ,  C12N9/16 ,  C12P7/6463

Abstract: The invention features plant acyl-ACP thioesterase genes of the FatB class and proteins encoded by these genes. The genes are useful for constructing recombinant host cells having altered fatty acid profiles. Oleaginous microalga host cells with the new genes or previously identified FatB genes are disclosed. The microalgae cells produce triglycerides with useful fatty acid profiles.

Abstract translation: 本发明具有FatB类的植物酰基-ACP硫酯酶基因和由这些基因编码的蛋白质。 该基因可用于构建具有改变的脂肪酸谱的重组宿主细胞。 公开了具有新基因或先前鉴定的FatB基因的嗜酸微藻宿主细胞。 微藻细胞产生具有有用脂肪酸分布的甘油三酯。