-
21.发明申请公开(公告)号：US20140113367A1
公开(公告)日：2014-04-24
申请号：US13855413
申请日：2013-04-02
发明人： Karl Deisseroth , Feng Zhang , Viviana Gradinaru
IPC分类号： C07K14/00
CPC分类号： A61N5/062 , A01K67/0333 , A01K2217/052 , A01K2227/703 , A01K2267/0356 , A61K38/00 , A61K48/005 , A61K48/0058 , A61K48/0083 , A61N5/0613 , A61N5/0622 , C07K14/00 , C07K14/195 , C07K14/215 , C12N13/00 , C12N15/86 , C12N2710/10343 , C12N2710/16643 , C12N2740/16043 , C12N2740/16071 , C12N2750/14143 , C12N2830/002
摘要： Stimulation of target cells using light, e.g., in vivo or in vitro, is implemented using a variety of methods and devices. One example involves a vector for delivering a light-activated NpHR-based molecule comprising a nucleic acid sequence that codes for light-activated NpHR-based molecule and a promoter. Either a high expression of the molecule manifests a toxicity level that is less than about 75%, or the light-activated NpHR-based proteins are expressed using at least two NpHR-based molecular variants. Each of the variants characterized in being useful for expressing a light-activated NpHR-based molecule that responds to light by producing an inhibitory current to dissuade depolarization of the neuron. Other aspects and embodiments are directed to systems, methods, kits, compositions of matter and molecules for ion pumps or for controlling inhibitory currents in a cell (e.g., in in vivo and in vitro environments).
摘要翻译： 使用光，例如体内或体外，使用各种方法和装置来实施靶细胞的刺激。 一个实例涉及用于递送光激活的基于NpHR的分子的载体，其包含编码基于光激活的基于NpHR的分子和启动子的核酸序列。 分子的高表达表现出小于约75％的毒性水平，或者使用至少两种基于NpHR的分子变体表达光激活的NpHR基蛋白。 每个变体的特征在于可用于表达基于光激活的基于NpHR的分子，其通过产生抑制电流来抑制神经元的去极化来响应光。 其他方面和实施方案涉及用于离子泵的物质和分子的组合物，或用于控制细胞中的抑制性电流（例如在体内和体外环境中）的系统，方法，试剂盒，组合物。
-
公开(公告)号：US11007374B2
公开(公告)日：2021-05-18
申请号：US16448608
申请日：2019-06-21
发明人： Karl Deisseroth , Feng Zhang , Edward Boyden
摘要： Various systems and methods are implemented for controlling stimulus of a cell. One such method is implemented for optical stimulation of a cell expressing an NpHR ion pump. The method includes the step of providing a sequence of stimuli to the cell. Each stimulus increases the probability of depolarization events occurring in the cell. Light is provided to the cell to activate the expressed NpHR ion pump, thereby decreasing the probability of depolarization events occurring in the cell.
-
公开(公告)号：US20200072817A1
公开(公告)日：2020-03-05
申请号：US16562176
申请日：2019-09-05
摘要： A variety of applications, systems, methods and constructs are implemented for use in connection with screening of ion-channel modulators. Consistent with one such system, drug candidates are screened to identify their effects on cell membrane ion channels and pumps. The system includes screening cells having light responsive membrane ion switches, voltage-gated ion switches and fluorescence producing voltage sensors. A chemical delivery device introduces the drug candidates to be screened. An optical delivery device activates the light responsive ion switches. An optical sensor monitors fluorescence produced by the voltage sensors. A processor processes data received from the optical sensor. A memory stores the data received from the optical sensor.
-
公开(公告)号：US10451608B2
公开(公告)日：2019-10-22
申请号：US15153305
申请日：2016-05-12
摘要： A variety of applications, systems, methods and constructs are implemented for use in connection with screening of ion-channel modulators. Consistent with one such system, drug candidates are screened to identify their effects on cell membrane ion channels and pumps. The system includes screening cells having light responsive membrane ion switches, voltage-gated ion switches and fluorescence producing voltage sensors. A chemical delivery device introduces the drug candidates to be screened. An optical delivery device activates the light responsive ion switches. An optical sensor monitors fluorescence produced by the voltage sensors. A processor processes data received from the optical sensor. A memory stores the data received from the optical sensor.
-
公开(公告)号：US20190071476A1
公开(公告)日：2019-03-07
申请号：US16115303
申请日：2018-08-28
发明人： Karl Deisseroth , Feng Zhang , Viviana Gradinaru
摘要： The present disclosure provides opsins, including variant opsins with increased activity and/or increased trafficking to the plasma membrane. The opsins are useful in therapeutic and screening applications, which are also provided.
-
公开(公告)号：US20180244737A1
公开(公告)日：2018-08-30
申请号：US15962773
申请日：2018-04-25
发明人： Karl Deisseroth , Feng Zhang , Viviana Gradinaru
IPC分类号： C07K14/47
CPC分类号： C07K14/47 , A61K38/00 , A61K48/00 , A61K48/005 , A61N5/06 , C07K14/415 , C07K2319/03 , C07K2319/04 , C07K2319/60 , C12N13/00
摘要： The present disclosure provides opsins, including variant opsins with increased activity and/or increased trafficking to the plasma membrane. The opsins are useful in therapeutic and screening applications, which are also provided.
-
公开(公告)号：US20170348545A1
公开(公告)日：2017-12-07
申请号：US15623081
申请日：2017-06-14
发明人： Karl Deisseroth , Feng Zhang , Edward Boyden
CPC分类号： A61N5/0622 , A61N1/36 , A61N5/06 , A61N5/0601 , A61N5/0613 , A61N2005/0626 , A61N2005/0647 , A61N2005/0652 , A61N2005/0663 , A61N2005/0665 , C12N5/0602 , C12N13/00 , G01N33/502 , G01N33/6872
摘要： Various systems and methods are implemented for controlling stimulus of a cell. One such method is implemented for optical stimulation of a cell expressing a NpHR ion pump. The method includes the step of providing a sequence of stimuli to the cell. Each stimulus increases the probability of depolarization events occurring in the cell. Light is provided to the cell to activate the expressed NpHR ion pump, thereby decreasing the probability of depolarization events occurring in the cell.
-
公开(公告)号：US20160258929A1
公开(公告)日：2016-09-08
申请号：US15153305
申请日：2016-05-12
CPC分类号： G01N33/502 , A61N5/0622 , B01J2219/00315 , B01J2219/00495 , B01J2219/00702 , C40B60/12 , G01N33/5008 , G01N33/6872
摘要： A variety of applications, systems, methods and constructs are implemented for use in connection with screening of ion-channel modulators. Consistent with one such system, drug candidates are screened to identify their effects on cell membrane ion channels and pumps. The system includes screening cells having light responsive membrane ion switches, voltage-gated ion switches and fluorescence producing voltage sensors. A chemical delivery device introduces the drug candidates to be screened. An optical delivery device activates the light responsive ion switches. An optical sensor monitors fluorescence produced by the voltage sensors. A processor processes data received from the optical sensor. A memory stores the data received from the optical sensor.
-
公开(公告)号：US20160222073A1
公开(公告)日：2016-08-04
申请号：US15097925
申请日：2016-04-13
发明人： Karl Deisseroth , Feng Zhang
CPC分类号： C07K14/47 , A61K38/00 , A61K48/00 , A61K48/005 , A61N5/06 , C07K14/415 , C07K2319/03 , C07K2319/04 , C07K2319/60 , C12N13/00
摘要： The present disclosure provides opsins, including variant opsins with increased activity and/or increased trafficking to the plasma membrane. The opsins are useful in therapeutic and screening applications, which are also provided.
摘要翻译： 本公开提供了操作，包括具有增加的活性和/或增加对质膜的运输的变体蛋白质。 操作素在治疗和筛选应用中也是有用的，也是提供的。
-
30.发明授权Methods for treating parkinson's disease by optically stimulating target cells 有权通过光学刺激靶细胞治疗帕金森病的方法公开(公告)号：US09394347B2
公开(公告)日：2016-07-19
申请号：US14694845
申请日：2015-04-23
发明人： Karl Deisseroth , Feng Zhang , Viviana Gradinaru
IPC分类号： C07K14/405 , C07K14/47 , C12N15/75 , C12N15/85 , C12N15/86 , C12N7/00 , A61N5/06 , A61K41/00 , A61K48/00 , A61K31/713 , C12N15/79 , A61N5/067
CPC分类号： A61N5/062 , A61K31/713 , A61K41/0057 , A61K48/005 , A61K48/0058 , A61K48/0083 , A61N5/0601 , A61N2005/063 , A61N2005/0651 , A61N2005/0662 , A61N2005/067 , C07K14/405 , C07K14/47 , C12N7/00 , C12N15/79 , C12N15/85 , C12N15/86 , C12N2740/10043 , C12N2740/15043 , C12N2740/15045 , C12N2750/14143
摘要： Methods, systems and devices are implemented in connection with light-responsive ion channel molecules. One such method is implemented using a light-activated ion channel molecule that responds to a light stimulus. The method includes engineering the light-activated ion channel molecule in a cell; and activating the ion channel molecule, in response to light stimulus that is provided to the ion channel molecule and that has properties that do not activate a ChR2 ion channel, to allow ions to pass through the light-activated ion channel molecule.
摘要翻译： 结合光响应离子通道分子实现方法，系统和装置。 使用响应于光刺激的光激活离子通道分子来实现一种这样的方法。 该方法包括在细胞中工程化光活化离子通道分子; 以及响应于提供给所述离子通道分子的具有不激活ChR2离子通道的性质的光刺激，激活离子通道分子，以允许离子通过光活化的离子通道分子。
-
-
-
-
-
-
-
-
-
搜索结果
55 条记录 (耗时 0.048 秒)
