公开(公告)号：US20100167281A1

公开(公告)日：2010-07-01

申请号：US12389151

申请日：2009-02-19

申请人： Maiko Tanabe ,  Chihiro Uematsu ,  Hirokazu Nishida ,  Kenko Uchida

发明人： Maiko Tanabe ,  Chihiro Uematsu ,  Hirokazu Nishida ,  Kenko Uchida

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6816 ,  C12Q2521/501 ,  C12Q2565/301

摘要： It is intended to provide an assay for the presence, absence or amount of a nucleic-acid fragment having a certain nucleotide sequence, for example, a polyA length, a difference in the number of repetition of a direct repeat sequence (e.g., microsatellite), single nucleotide substitution (or single nucleotide polymorphism), and nucleotide sequence insertion or deletion, and to provide a genetic testing using the same. The present invention relates to a nucleotide analysis method, comprising: hybridizing at least two probes to a nucleic-acid fragment; ligating the at least two probes using ligase; exchanging, to ATP, pyrophosphoric acid produced through the ligation reaction; and detecting chemiluminescence reaction dependent on the ATP.

摘要翻译： 旨在提供具有某一核苷酸序列的核酸片段的存在，不存在或量的测定，例如polyA长度，直接重复序列（例如，微卫星）的重复次数的差异， ，单核苷酸取代（或单核苷酸多态性）和核苷酸序列插入或缺失，并提供使用其的遗传检测。 本发明涉及一种核苷酸分析方法，包括：将至少两个探针与核酸片段杂交; 使用连接酶连接至少两个探针; 通过连接反应产生的ATP，焦磷酸交换; 并根据ATP检测化学发光反应。

公开(公告)号：US20090189063A1

公开(公告)日：2009-07-30

申请号：US12330374

申请日：2008-12-08

申请人： Akihiro Sano ,  Atsumu Hirabayashi ,  Yasushi Terui ,  Kinya Kobayashi ,  Kiyomi Yoshinari ,  Kenko Uchida ,  Toshiyuki Yokosuka

发明人： Akihiro Sano ,  Atsumu Hirabayashi ,  Yasushi Terui ,  Kinya Kobayashi ,  Kiyomi Yoshinari ,  Kenko Uchida ,  Toshiyuki Yokosuka

IPC分类号： H01J49/00

CPC分类号： G01N33/6851 ,  G01N33/6848 ,  H01J49/0031 ,  H01J49/0036 ,  H01J49/004

摘要： During the structural analysis of a protein or peptide by tandem mass spectroscopy, a peptide ion derived from a protein that has already been measured and that is expressed in great quantities is avoided as a tandem mass spectroscopy target. A peptide derived from a minute amount of protein, which has heretofore been difficult to analyze, can be automatically determined as a tandem mass spectroscopy target within the real time of measurement. Data concerning a protein that has already been measured and a peptide derived from the protein is automatically stored in an internal database. The stored data is collated with measured data with high accuracy to determine an isotope peak. In this way, the process of selecting a peptide peak that has not been measured as the target for the next tandem analysis can be performed within the real time of measurement and a redundant measurement of peptides derived from the same protein can be avoided. The information contained in the MSn spectrum is effectively utilized in each step of the MSn involving a multi-stage dissociation and mass spectroscopy (MSn), so that the flows for the determination of the next analysis content and the selection of the parent ion for the MSn+1 analysis, for example, can be optimized within the real time of measurement and with high efficiency and accuracy. Thus, a target of concern to the user can be subjected to tandem mass spectroscopy without wasteful measurement.

摘要翻译： 在通过串联质谱法对蛋白质或肽进行结构分析期间，作为串联质谱目标，可以避免衍生自已经测量并且大量表达的蛋白质的肽离子。 迄今为止难以分析的，从微量蛋白质衍生的肽可以在实时测定中作为串联质谱图目标自动确定。 关于已经测量的蛋白质和源自蛋白质的肽的数据被自动存储在内部数据库中。 存储的数据与测量数据高精度对照以确定同位素峰。 以这种方式，可以在测量的实际时间内进行未被测定为下一个串联分析的靶标的肽峰的过程，并且可以避免衍生自相同蛋白质的肽的冗余测量。 包含在MSn谱中的信息在涉及多级解离和质谱（MSn）的MSn的每个步骤中被有效地利用，使得用于确定下一个分析内容的流程和为母体离子的选择 例如，MSn + 1分析可以在实时测量和高效率和精确度下进行优化。 因此，用户关注的目标可以进行串联质谱，无需浪费测量。

公开(公告)号：US20050123965A1

公开(公告)日：2005-06-09

申请号：US10981521

申请日：2004-11-05

申请人： Yoshihiro Yamashita ,  Toshinari Sakurai ,  Norihito Kuno ,  Kenko Uchida ,  Toshiaki Yokobayashi

发明人： Yoshihiro Yamashita ,  Toshinari Sakurai ,  Norihito Kuno ,  Kenko Uchida ,  Toshiaki Yokobayashi

IPC分类号： C12N15/09 ,  C12N15/10 ,  C12Q1/68 ,  C07H21/04

CPC分类号： C12N15/1006 ,  C12N15/101

摘要： A method to extract RNA with high purity from biological materials containing RNA in a safe, rapid, and simple procedure and a method to analyze it are provided. The procedure includes the steps of mixing a biological material containing RNA with a predetermined concentration of a chaotropic agent and a predetermined concentration of an organic solvent, allowing the mixed solution to contact a nucleic acid-binding solid phase, washing the nucleic-acid binding solid-phase to which RNA is bound, and eluting RNA from the nucleic-acid binding solid-phase having the bound RNA. Furthermore, the obtained RNA is analyzed by reverse transcriptase-polymerase chain reaction (RT-PCR) or the like.

摘要翻译： 提供了一种以安全，快速，简单的方法从含有RNA的生物材料中提取高纯度RNA的方法和分析方法。 该方法包括以下步骤：将含有预定浓度的离液剂和预定浓度的有机溶剂的含有RNA的生物材料混合，使混合溶液与核酸结合固相接触，洗涤核酸结合固体 RNA结合的RNA，并从具有结合RNA的核酸结合固相中洗脱RNA。 此外，通过逆转录酶 - 聚合酶链反应（RT-PCR）等分析得到的RNA。

公开(公告)号：US06756014B2

公开(公告)日：2004-06-29

申请号：US10022735

申请日：2001-12-20

申请人： Michael Himmelhaus ,  Kenko Uchida ,  Hiroyuki Takei

发明人： Michael Himmelhaus ,  Kenko Uchida ,  Hiroyuki Takei

IPC分类号： G01N3348

CPC分类号： G01N33/54313 ,  Y10T436/25 ,  Y10T436/255

摘要： The invention provides a biochemical sensor with probes uniformly caught in each section. The probes used for detecting a substance of interest are caught in advance on particles, and the particles are fixed in each of sections arranged in form of lattice using a chemical patterning method on the surface of a baseplate. In each section, the particles attached with probes caught on the surface are fixed in single layer and tightly packed. The quantity of the particles fixed on the baseplate is determined by using a light scattering from the particles or by labeling the particles in advance with fluorescent substance. Therefore, the number of probes caught in each section of individual biochemical sensor is determined so as to allow the substance of interest to be detected with high accuracy.

摘要翻译： 本发明提供一种生物化学传感器，其中每个部分均匀地夹有探针。 用于检测感兴趣物质的探针被预先捕获在颗粒上，并且在基板的表面上使用化学图案化方法将颗粒固定在格子形式的每个部分中。 在每个部分中，附着在表面上的探针附着的颗粒固定在单层中并紧密包装。 通过使用来自颗粒的光散射或通过用荧光物质预先标记颗粒来确定固定在基板上的颗粒的量。 因此，确定各个生物化学传感器的每个部分中捕获的探针的数量，以便能高精度地检测感兴趣的物质。

公开(公告)号：US06216538B1

公开(公告)日：2001-04-17

申请号：US08742695

申请日：1996-11-04

申请人： Kenji Yasuda ,  Shin-ichiro Umemura ,  Kenichi Kawabata ,  Kazuo Takeda ,  Kenko Uchida ,  Yoshinori Harada ,  Masao Kamahori ,  Kazuaki Sasaki

发明人： Kenji Yasuda ,  Shin-ichiro Umemura ,  Kenichi Kawabata ,  Kazuo Takeda ,  Kenko Uchida ,  Yoshinori Harada ,  Masao Kamahori ,  Kazuaki Sasaki

IPC分类号： G01H1700

CPC分类号： B01D21/283 ,  G10K15/00

摘要： An ultrasonic manipulation apparatus has a plurality of ultrasonic wave oscillators arranged in two dimensions to trap, fix or move particles to an optional position in the solution or perform cell fusion by using a gradient force obtained by superposing one over another the gradient force fields generated by ultrasonic waves produced by a plurality of ultrasonic wave oscillators. The ultrasonic wave oscillators, functioning independently of one another, can emit ultrasonic waves with optional intensities and phases, and by using an external force produced by superposed gradient force fields generated by ultrasonic waves, particles are handled easily.

摘要翻译： 超声波操作装置具有多个超声波振荡器，其被设置成二维排列，以将颗粒捕获，固定或移动到溶液中的可选位置，或者通过使用通过将颗粒叠加在另一个上而产生的梯度力场而获得的梯度力进行细胞融合 由多个超声波振荡器产生的超声波。 彼此独立起作用的超声波振荡器可以发射具有可选强度和相位的超声波，并且通过使用由超声波产生的叠加梯度力场产生的外力，可以容易地处理颗粒。

公开(公告)号：US20090069663A1

公开(公告)日：2009-03-12

申请号：US12267056

申请日：2008-11-07

申请人： Akihiko Kandori ,  Tsuyoshi Miyashita ,  Keiji Tsukada ,  Kenko Uchida ,  Hideo Kawaguchi ,  Minoru Sakairi

发明人： Akihiko Kandori ,  Tsuyoshi Miyashita ,  Keiji Tsukada ,  Kenko Uchida ,  Hideo Kawaguchi ,  Minoru Sakairi

IPC分类号： A61B5/05

CPC分类号： A61B5/1101 ,  A61B5/4082 ,  A61B5/6824 ,  A61B5/6826 ,  A61B5/6828 ,  A61B5/6838 ,  A61B5/7239

摘要： A living body inspection apparatus including an oscillation coil which passes an AC current, a detection coil which detects an AC magnetic field generated from the detection coil, an amplification circuit which amplifies a voltage generated by the magnetic field induced by the detection coil, a detecting unit for detecting the output signal of the amplification circuit, a low pass filter to which the output signal of the detecting unit is input, a unit for setting the oscillation coil and detection coil in first and second regions of the living body, a recording unit for recording the output of the low pass filter while the first region and the second region of the living body are moving and a displaying unit for displaying the data recorded in the recording unit or results of analysis of the recorded data.

摘要翻译： 一种生物体检查装置，包括通过交流电流的振荡线圈，检测由检测线圈产生的交流磁场的检测线圈，放大电路，放大电路，放大由检测线圈感应出的磁场产生的电压， 用于检测放大电路的输出信号的单元，输入检测单元的输出信号的低通滤波器，用于在生物体的第一和第二区域设置振荡线圈和检测线圈的单元，记录单元 用于在活体的第一区域和第二区域移动时记录低通滤波器的输出，以及用于显示记录在记录单元中的数据的显示单元或记录数据的分析结果。

公开(公告)号：US20090020555A1

公开(公告)日：2009-01-22

申请号：US12084990

申请日：2006-03-07

申请人： Hideyuki Noda ,  Yoshinobu Kohara ,  Kenko Uchida

发明人： Hideyuki Noda ,  Yoshinobu Kohara ,  Kenko Uchida

IPC分类号： B67D5/08

CPC分类号： G01N35/1016 ,  B01F13/0071 ,  B01J19/0046 ,  B01J2219/00315 ,  B01J2219/00369 ,  B01J2219/00376 ,  B01J2219/00468 ,  B01J2219/005 ,  B01J2219/00648 ,  B01L3/0241 ,  B01L3/0289 ,  B01L2200/0605 ,  B01L2200/0647 ,  B01L2200/0673 ,  B01L2300/0838 ,  B01L2400/0487 ,  G01N2035/00574 ,  G01N2035/1046

摘要： The present invention provides an apparatus for efficiently transporting or dispensing transport objects including not only particles but also liquid samples. A liquid in a first liquid transport pipe (3) is fed at a liquid feed velocity (V1), and a liquid droplet is formed toward an open end of a second liquid transport pipe (4) disposed with an air gap (11) in between. The particle is released into the liquid droplet, so that the particle is enclosed in the liquid droplet. Suction with a liquid feed velocity (V2) is applied to the inside of the second liquid transport pipe. Since the relationship between V1 and V2 is V1

摘要翻译： 本发明提供了一种用于有效地运输或分配运输物体的装置，不仅包括颗粒而且包括液体样品。 第一液体输送管（3）中的液体以液体进料速度（V1）进给，并且朝向设置有气隙（11）的第二液体输送管（4）的开口端形成液滴 之间。 颗粒被释放到液滴中，使得颗粒被包围在液滴中。 利用液体进料速度（V2）进行吸入被施加到第二液体输送管的内部。 由于V1和V2之间的关系为V1

公开(公告)号：US07473892B2

公开(公告)日：2009-01-06

申请号：US10849517

申请日：2004-05-20

申请人： Akihiro Sano ,  Atsumu Hirabayashi ,  Yasushi Terui ,  Kinya Kobayashi ,  Kiyomi Yoshinari ,  Kenko Uchida ,  Toshiyuki Yokosuka

发明人： Akihiro Sano ,  Atsumu Hirabayashi ,  Yasushi Terui ,  Kinya Kobayashi ,  Kiyomi Yoshinari ,  Kenko Uchida ,  Toshiyuki Yokosuka

IPC分类号： H01J49/26 ,  B01D59/44

CPC分类号： G01N33/6851 ,  G01N33/6848 ,  H01J49/0031 ,  H01J49/0036 ,  H01J49/004

摘要： During the structural analysis of a protein or peptide by tandem mass spectroscopy, a peptide ion derived from a protein that has already been measured and that is expressed in great quantities is avoided as a tandem mass spectroscopy target. A peptide derived from a minute amount of protein, which has heretofore been difficult to analyze, can be automatically determined as a tandem mass spectroscopy target within the real time of measurement. Data concerning a protein that has already been measured and a peptide derived from the protein is automatically stored in an internal database. The stored data is collated with measured data with high accuracy to determine an isotope peak. In this way, the process of selecting a peptide peak that has not been measured as the target for the next tandem analysis can be performed within the real time of measurement and a redundant measurement of peptides derived from the same protein can be avoided. The information contained in the MSn spectrum is effectively utilized in each step of the MSn involving a multi-stage dissociation and mass spectroscopy (MSn), so that the flows for the determination of the next analysis content and the selection of the parent ion for the MSn+1 analysis, for example, can be optimized within the real time of measurement and with high efficiency and accuracy. Thus, a target of concern to the user can be subjected to tandem mass spectroscopy without wasteful measurement.

摘要翻译： 在通过串联质谱法对蛋白质或肽进行结构分析期间，作为串联质谱目标，可以避免衍生自已经测量并且大量表达的蛋白质的肽离子。 迄今为止难以分析的，从微量蛋白质衍生的肽可以在实时测定中作为串联质谱图目标自动确定。 关于已经测量的蛋白质和来自蛋白质的肽的数据被自动存储在内部数据库中。 存储的数据与测量数据高精度对照以确定同位素峰。 以这种方式，可以在测量的实际时间内进行未被测定为下一个串联分析的靶标的肽峰的过程，并且可以避免衍生自相同蛋白质的肽的冗余测量。 包含在MSn谱中的信息在涉及多级解离和质谱（MSn）的MSn的每个步骤中被有效地利用，使得用于确定下一个分析内容的流程和为母体离子的选择 例如，MSn + 1分析可以在实时测量和高效率和精确度下进行优化。 因此，用户关注的目标可以进行串联质谱，无需浪费测量。

公开(公告)号：US20080261294A1

公开(公告)日：2008-10-23

申请号：US12034880

申请日：2008-02-21

申请人： Hideyuki Noda ,  Satoshi Ozawa ,  Masahiro Okanojo ,  Kenko Uchida

发明人： Hideyuki Noda ,  Satoshi Ozawa ,  Masahiro Okanojo ,  Kenko Uchida

IPC分类号： G01N21/76 ,  C12M1/36

CPC分类号： C12Q1/04 ,  G01N21/76

摘要： An apparatus includes a system for guiding chemiluminescence and a system for preventing a variation in dark currents. The apparatus includes a first light shielding BOX having a sample container holder and a shutter unit therein, the shutter unit including a top plate which is partly formed by a movement of a plate member and a second light shielding BOX having a photodetector therein. While a measurement is not implemented, the shutter unit is closed to block entrance of stray light to the photodetector, and while a measurement is implemented, the plate member is moved to open the shutter unit and the tip of the photodetector is inserted into a through hole formed in the top plate, so that the distance between the bottom of the sample container and a sensitive area of the photodetector is reduced to several millimeters or less.

摘要翻译： 一种装置包括用于引导化学发光的系统和用于防止暗电流变化的系统。 该装置包括：第一遮光盒，其具有样品容器保持器和其中的快门单元，所述快门单元包括顶板，该顶板部分地通过板构件的运动和在其中具有光电检测器的第二遮光箱而形成。 虽然没有实现测量，但是关闭快门单元以阻止杂散光入射到光电检测器，并且在实施测量的同时，移动板构件以打开快门单元，并且将光电检测器的末端插入通孔 使得样品容器的底部与光电检测器的敏感区域之间的距离减小到几毫米或更小。

公开(公告)号：US20060122477A1

公开(公告)日：2006-06-08

申请号：US11070816

申请日：2005-03-03

申请人： Yukiko Hirabayashi ,  Kenko Uchida

发明人： Yukiko Hirabayashi ,  Kenko Uchida

IPC分类号： A61B5/00

CPC分类号： A61B5/6814 ,  A61B5/0042 ,  A61B5/0261 ,  A61B5/14553 ,  A61B2562/0233 ,  A61B2562/046

摘要： Disclosed is a probe for biomeasurement by use of light capable of adjusting positions of incident points and detection points without changing the distance between the incident point and the detection point in accordance with the size of a subject's head, and an optical bioinstrumentation for living body using the probe. The distances between the incident points and the detection points are approximately the same. A gap is formed by removing part of connecting members, rotatable about the incident point and the detection point, around the subject head top portion. The size of the probe is changed by changing the distance between the incident points or the detection points around the gap.

摘要翻译： 公开了一种用于通过使用能够调整入射点和检测点的位置的光而不改变入射点与检测点之间的距离的光的探针，该探针根据受检者的头部的尺寸和使用的生物体的光学生物仪器 探针。 入射点和检测点之间的距离大致相同。 通过将围绕入射点和检测点旋转的连接构件的一部分围绕被检体顶部部分去除而形成间隙。 通过改变入射点或间隙周围的检测点之间的距离来改变探头的尺寸。