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    ISOLATED ALCOHOL DEHYDROGENASE ENZYMES AND USES THEREOF
    21.
    发明申请
    ISOLATED ALCOHOL DEHYDROGENASE ENZYMES AND USES THEREOF 审中-公开
    分离的醇酒精脱乙酰壳多糖及其用途

    公开(公告)号：US20090203089A1

    公开(公告)日：2009-08-13

    申请号：US12361293

    申请日：2009-01-28

    申请人： Yuki Kashiyama

    发明人： Yuki Kashiyama

    IPC分类号： C12P19/02 ,  C12N15/53 ,  C12N15/70 ,  C12N15/75 ,  C12N9/04 ,  C12P7/06 ,  C12P19/00 ,  C12N1/21 ,  C12N1/15 ,  C12N1/19 ,  C12N15/76 ,  C12N15/77 ,  C12N15/78 ,  C12N15/80 ,  C12N15/81 ,  C12N9/88

    CPC分类号： C12N9/0006 ,  C12P5/00 ,  C12P7/00 ,  C12P7/06 ,  C12P7/58 ,  C12P19/02 ,  Y02E50/17 ,  Y02T50/678

    摘要： Bacterial polynucleotides and polypeptides are provided in which the polypeptides have a dehydrogenase activity, such as an alcohol dehydrogenase (ADH) activity, an uronate, a 4-deoxy-L-erythro-5-hexoseulose uronate (DEHU) ((4S,5S)-4,5 dihydroxy-2,6-dioxohexanoate) hydrogenase activity, a 2-keto-3-deoxy-D-gluconate dehydrogenase activity, a D-mannuronate hydrogenase activity, and/or a D-mannnonate dehydrogenase activity. Methods, enzymes, recombinant microorganism, and microbial systems are also provided for converting polysaccharides, such as those derived from biomass, into suitable monosaccharides or oligosaccharides, as well as for converting suitable monosaccharides or oligosaccharides into commodity chemicals, such as biofuels. Commodity chemicals produced by the methods described herein are also provided.

    摘要翻译： 提供了细菌多核苷酸和多肽，其中多肽具有脱氢酶活性，如醇脱氢酶（ADH）活性，糖醛酸酯，4-脱氧-L-赤藓-5-己糖糖酸糖苷（DEHU）（（4S，5S） -4,5-二羟基-2,6-二氧代己酸）氢化酶活性，2-酮-3-脱氧-D-葡萄糖酸脱氢酶活性，D-甘露糖醛酸盐酸酶活性和/或D-甘露糖酸脱氢酶活性。 还提供了方法，酶，重组微生物和微生物系统用于将多糖（例如衍生自生物质的那些）转化为合适的单糖或寡糖，以及将合适的单糖或寡糖转化为商品化学品，例如生物燃料。 还提供了通过本文所述方法生产的商品化学品。

    Isolated fungal promoters and gene transcription terminators and methods of protein and chemical production in a fungus
    23.
    发明授权
    Isolated fungal promoters and gene transcription terminators and methods of protein and chemical production in a fungus 失效
    分离的真菌启动子和基因转录终止子以及真菌中蛋白质和化学生产的方法

    公开(公告)号：US07449569B2

    公开(公告)日：2008-11-11

    申请号：US10920625

    申请日：2004-08-17

    申请人： Ziyu Dai ,  Linda L. Lasure ,  Jon K. Magnuson

    发明人： Ziyu Dai ,  Linda L. Lasure ,  Jon K. Magnuson

    IPC分类号： C07H21/04 ,  C12Q1/68 ,  C12N15/00 ,  C12N15/63 ,  C12N1/00

    CPC分类号： C12P7/44 ,  C07K14/38 ,  C12N15/80 ,  C12P7/46 ,  C12P7/48 ,  C12P7/56 ,  C12P7/58 ,  C12P17/06 ,  C12P21/005 ,  C12P35/00 ,  C12P37/00

    摘要： The present invention encompasses isolated gene regulatory elements and gene transcription terminators that are differentially expressed in a native fungus exhibiting a first morphology relative to the native fungus exhibiting a second morphology. The invention also encompasses a method of utilizing a fungus for protein or chemical production. A transformed fungus is produced by transforming a fungus with a recombinant polynucleotide molecule. The recombinant polynucleotide molecule contains an isolated polynucleotide sequence linked operably to another molecule comprising a coding region of a gene of interest. The gene regulatory element and gene transcription terminator may temporally and spatially regulate expression of particular genes for optimum production of compounds of interest in a transgenic fungus.

    摘要翻译： 本发明包括在天然真菌中差异表达的分离的基因调控元件和基因转录终止子，其表现出相对于表现出第二形态的天然真菌的第一形态。 本发明还包括利用真菌进行蛋白质或化学生产的方法。 通过用重组多核苷酸分子转化真菌产生转化的真菌。 重组多核苷酸分子含有与包含感兴趣的基因的编码区的另一个分子可操作地连接的分离的多核苷酸序列。 基因调控元件和基因转录终止子可以在时间上和空间上调节特定基因的表达，以在转基因真菌中最佳产生感兴趣的化合物。

    Isolated fungal promoters and gene transcription terminators and methods of protein and chemical production in a fungus
    25.
    发明申请
    Isolated fungal promoters and gene transcription terminators and methods of protein and chemical production in a fungus 失效
    分离的真菌启动子和基因转录终止子以及真菌中蛋白质和化学生产的方法

    公开(公告)号：US20060040342A1

    公开(公告)日：2006-02-23

    申请号：US10920625

    申请日：2004-08-17

    申请人： Ziyu Dai ,  Linda Lasure ,  Jon Magnuson

    发明人： Ziyu Dai ,  Linda Lasure ,  Jon Magnuson

    IPC分类号： C12P37/00 ,  C12P21/06 ,  C12P17/06 ,  C12P7/62 ,  C12P7/46 ,  C12N1/18 ,  C12N15/74

    CPC分类号： C12P7/44 ,  C07K14/38 ,  C12N15/80 ,  C12P7/46 ,  C12P7/48 ,  C12P7/56 ,  C12P7/58 ,  C12P17/06 ,  C12P21/005 ,  C12P35/00 ,  C12P37/00

    摘要： The present invention encompasses isolated gene regulatory elements and gene transcription terminators that are differentially expressed in a native fungus exhibiting a first morphology relative to the native fungus exhibiting a second morphology. The invention also encompasses a method of utilizing a fungus for protein or chemical production. A transformed fungus is produced by transforming a fungus with a recombinant polynucleotide molecule. The recombinant polynucleotide molecule contains an isolated polynucleotide sequence linked operably to another molecule comprising a coding region of a gene of interest. The gene regulatory element and gene transcription terminator may temporally and spatially regulate expression of particular genes for optimum production of compounds of interest in a transgenic fungus.

    摘要翻译： 本发明包括在天然真菌中差异表达的分离的基因调控元件和基因转录终止子，其表现出相对于表现出第二形态的天然真菌的第一形态。 本发明还包括利用真菌进行蛋白质或化学生产的方法。 通过用重组多核苷酸分子转化真菌产生转化的真菌。 重组多核苷酸分子含有与包含感兴趣的基因的编码区的另一个分子可操作地连接的分离的多核苷酸序列。 基因调控元件和基因转录终止子可以在时间上和空间上调节特定基因的表达，以在转基因真菌中最佳产生感兴趣的化合物。

    Method for preparing calcium gluconate
    28.
    发明申请
    Method for preparing calcium gluconate 有权
    制备葡萄糖酸钙的方法

    公开(公告)号：US20040253345A1

    公开(公告)日：2004-12-16

    申请号：US10491197

    申请日：2004-08-10

    发明人： Marcus Johannes Anthonius Wilhemus Vorage ,  Diderick Reinder Kremer ,  Boelem Sloots ,  Johannes Bernardus Maria Meiberg

    IPC分类号： A23K001/175

    CPC分类号： C12N9/0065 ,  C12N9/0006 ,  C12P7/58

    摘要： The invention provides for a method for the preparation of a gluconic acid from glucose. It is an aspect of the invention to provide a method that comprises the use of enzymes. The method according to the invention can be applied with great benefit to the production of calcium gluconate suitable for use as a food fortifier by a process that is economical.

    摘要翻译： 本发明提供从葡萄糖制备葡萄糖酸的方法。 本发明的一个方面是提供一种包括使用酶的方法。 根据本发明的方法可以非常有利于通过经济的方法生产适合用作食物增强剂的葡萄糖酸钙。

    Method for producing ascorbic acid intermediates
    29.
    发明申请
    Method for producing ascorbic acid intermediates 审中-公开
    生产抗坏血酸中间体的方法

    公开(公告)号：US20040180413A1

    公开(公告)日：2004-09-16

    申请号：US10626033

    申请日：2003-07-23

    发明人： Matthew Grant Boston ,  Barbara A. Swanson

    IPC分类号： C12P017/04

    CPC分类号： C12P7/60 ,  C12P7/58

    摘要： The present invention relates to non-fermentative methods for the production of ASA intermediates, KDG, DKG and KLG and methods for the regeneration of co-factor. The invention provides genetically engineered host cells comprising heterologous nucleic acid encoding enzymes useful in the process.

    摘要翻译： 本发明涉及用于生产ASA中间体，KDG，DKG和KLG的非发酵方法以及辅因子再生的方法。 本发明提供了包含可用于该方法的异源核酸编码酶的经遗传工程化的宿主细胞。

    Methods for producing ethanol from carbon substrates
    30.
    发明申请
    Methods for producing ethanol from carbon substrates 审中-公开
    从碳底物生产乙醇的方法

    公开(公告)号：US20030180900A1

    公开(公告)日：2003-09-25

    申请号：US10360010

    申请日：2003-02-06

    发明人： Oreste Lantero

    IPC分类号： C12P007/16 ,  C12P007/06

    CPC分类号： C12P7/06 ,  C12P7/10 ,  C12P7/18 ,  C12P7/20 ,  C12P7/44 ,  C12P7/56 ,  C12P7/58 ,  C12P7/60 ,  Y02E50/16 ,  Y02E50/17

    摘要： The present invention provides means for the production of desired end-products of in vitro and/or in vivo bioconversion of biomass-based feed stock substrates, including but not limited to such materials as starch and cellulose. In particularly preferred embodiments, the methods of the present invention do not require gelatinization and/or liquefaction of the substrate. In particularly preferred embodiments, the present invention provides means for the production of ethanol.

    摘要翻译： 本发明提供了生产基于生物质的原料基质的体外和/或体内生物转化所需的终产物的方法，包括但不限于淀粉和纤维素等材料。 在特别优选的实施方案中，本发明的方法不需要基质的凝胶化和/或液化。 在特别优选的实施方案中，本发明提供了用于生产乙醇的方法。