公开(公告)号：US20010050254A1

公开(公告)日：2001-12-13

申请号：US09226941

申请日：1999-01-08

发明人： CHRISTOPHER J. WELCH ,  MARINA PROTOPOPOVA ,  BHAT GANAPATI

IPC分类号： B01D015/08

CPC分类号： B01J20/3261 ,  B01D15/08 ,  B01J20/286 ,  B01J20/3242 ,  B01J20/3253 ,  B01J2219/00283 ,  B01J2219/00308 ,  B01J2220/54 ,  C40B60/14 ,  G01N30/48 ,  G01N30/89

摘要： The invention discloses a method for rapid identification of a candidate selective separation material by placing small samples of the candidate material in an array of vials and adding a solution of the analytes to be separated. The solution is allowed to interact or equilibrate and the distribution of the analytes in the solid or liquid phase is measured usually by gas or liquid chromatography. The identified candidate material with the greatest differential adsorption of the analytes is selected and used as an adsorbent for large scale separation. The rapid screening of chromatographic adsorbents provides an efficient way of finding suitable absorbent materials for large scale separations.

摘要翻译： 本发明公开了一种通过将候选材料的小样品放置在小瓶阵列中并加入要分离的分析物的溶液来快速鉴定候选选择性分离材料的方法。 允许溶液相互作用或平衡，分析物在固相或液相中的分布通常通过气相色谱或液相色谱法测定。 选择具有最大差示吸附分析物的候选材料，并将其用作大规模分离的吸附剂。 色谱吸附剂的快速筛选提供了一种找到合适的大规模分离吸收材料的有效途径。

公开(公告)号：US5641893A

公开(公告)日：1997-06-24

申请号：US605733

申请日：1996-02-22

申请人： Lynn S. Penn ,  Donna L. Scott ,  Howard H. Weetall ,  Anne L. Plant ,  Marion F. McCurley ,  Scott A. Glazier

发明人： Lynn S. Penn ,  Donna L. Scott ,  Howard H. Weetall ,  Anne L. Plant ,  Marion F. McCurley ,  Scott A. Glazier

IPC分类号： G01N30/52 ,  G01N30/60 ,  G01N30/62 ,  G01N30/64 ,  G01N30/74 ,  G01N30/89

CPC分类号： G01N21/648 ,  G01N21/6428 ,  G01N21/7703 ,  G01N30/6069 ,  G01N30/62 ,  G01N2021/6484 ,  G01N2021/7786 ,  G01N2030/522 ,  G01N30/6021 ,  G01N30/6034 ,  G01N30/6082 ,  G01N30/64 ,  G01N30/74 ,  G01N30/89

摘要： An apparatus for the monitoring of a column chromatography separation process includes a segmented column with a seal positioned at the joint defined by the segments of the column. A connector is provided for connecting the segments of the column together. The apparatus further includes a sensor for monitoring an analyte in an eluant within a separation zone of the column. The sensor includes a mesh grid made of optical fibers or metal wires which is placed so as to extend through the separation zone of the column. The metal wires or optical fibers extend through the seal of the joint in the segmented column and connect to signal processing and data analysis equipment for purposes of monitoring the movements and concentration of an analyte in an eluant at various locations within the column. Certain segments of the optical fibers or metal wires which make up the mesh grid are coated so as to be desensitized and other segments are uncoated for sensing the analyte. This provides an effective apparatus to monitor in detail the cross-section of a column chromatography process in-situ.

摘要翻译： 用于监测柱色谱分离方法的装置包括分段柱，其具有位于由柱的段限定的接头处的密封。 提供连接器用于将柱的段连接在一起。 该装置还包括用于监测柱的分离区域内的洗脱液中的分析物的传感器。 传感器包括由光纤或金属线制成的网格格栅，该网格格栅被放置成延伸穿过柱的分离区。 金属线或光纤通过分段柱中的接头的密封件延伸，并连接到信号处理和数据分析设备，目的是监测柱内不同位置的洗脱液中分析物的运动和浓度。 构成网状网格的光纤或金属线的某些部分被涂覆以使其脱敏，并且其它部分未被涂覆以感测分析物。 这提供了一种现场详细监测柱色谱法的横截面的有效装置。

公开(公告)号：US11982652B2

公开(公告)日：2024-05-14

申请号：US17512160

申请日：2021-10-27

申请人： KOREA UNIVERSITY RESEARCH AND BUSINESS FOUNDATION

发明人： Sang-Won Lee ,  Hangyeore Lee

IPC分类号： G01N30/34 ,  B01D15/08 ,  B01D15/24 ,  B01D15/32 ,  G01N30/02 ,  G01N30/20 ,  G01N30/38 ,  G01N30/46 ,  G01N30/82 ,  G01N30/89

CPC分类号： G01N30/34 ,  B01D15/08 ,  G01N30/82 ,  G01N30/89 ,  B01D15/24 ,  B01D15/325 ,  G01N2030/027 ,  G01N2030/201 ,  G01N2030/202 ,  G01N2030/207 ,  G01N30/38 ,  G01N30/463

摘要： Disclosed are non-contiguous sample fractionating and concatenating device and a dual online multidimensional liquid chromatography system having the same. The non-contiguous sample fractionating and concatenating device according to an embodiment of the present disclosure includes a sample supply module which supplies a sample to be analyzed, and a sample fractionation module connected to the sample supply module, and which is continuously supplied with the sample, sets a plurality of unit sample supply times obtained by equally dividing a total sample supply time during which the sample is supplied from the sample supply module, sets a plurality of unit fractionation intervals obtained by equally dividing each of the plurality of unit sample supply times, and concatenates and stores the sample supplied during corresponding unit fractionation intervals within each unit sample supply time to acquire a plurality of fractions.

公开(公告)号：US20080299672A1

公开(公告)日：2008-12-04

申请号：US12096033

申请日：2006-11-17

申请人： Samuel Nochumson ,  Peter Levison

发明人： Samuel Nochumson ,  Peter Levison

IPC分类号： G01N30/02

CPC分类号： G01N30/89 ,  G01N30/6091 ,  G01N2030/889 ,  G01N2030/528 ,  G01N2030/527

摘要： Methods for testing the chromatography type and/or the integrity of a chromatography membrane or monolith, preferably, for testing the chromatography type and integrity of a chromatography device comprising a chromatography membrane or chromatography monolith while the membrane or monolith is sealed in a housing, are disclosed.

摘要翻译： 用于测试层析类型和/或色谱膜或整料的完整性的方法，优选用于在膜或整体密封在壳体中的情况下测试包括色谱膜或色谱单块的色谱装置的色谱类型和完整性， 披露

公开(公告)号：US06562627B1

公开(公告)日：2003-05-13

申请号：US09471854

申请日：1999-12-23

申请人： Charles Pidgeon ,  Jianming Yin ,  Nadege Rooke ,  Ling Han Morgan

发明人： Charles Pidgeon ,  Jianming Yin ,  Nadege Rooke ,  Ling Han Morgan

IPC分类号： G01N3002

CPC分类号： G01N30/96 ,  G01N30/02 ,  G01N30/34 ,  G01N30/72 ,  G01N30/7233 ,  G01N30/8637 ,  G01N30/89 ,  G01N2030/527 ,  Y10T436/16

摘要： Methods are provided for determining a dissociation constant for each one of at least a portion of a set of compounds. The methods include contacting a solution of the set of compounds with a membrane mimetic surface exhibiting a non-specific compound-dependent affinity for at least the portion of the compounds. A parameter dependent on the non-specific affinity of the surface for each one of at least the portion of the set of compounds in the test solution is measured and used to calculate the dissociation constant.

摘要翻译： 提供了用于确定一组化合物的至少一部分中的每一个的解离常数的方法。 所述方法包括使该组化合物的溶液与至少部分化合物表现出非特异性化合物依赖性亲和力的膜模拟表面接触。 测量取决于测试溶液中至少部分化合物的至少一部分中每一个表面的非特异性亲和力的参数，并用于计算解离常数。

公开(公告)号：US20030027356A1

公开(公告)日：2003-02-06

申请号：US10179809

申请日：2002-06-24

发明人： Miroslav Petro ,  Ralph B. Nielsen ,  Jacqueline M. Regan

IPC分类号： G01N033/53 ,  G01N033/558 ,  G01N033/543

CPC分类号： C40B30/04 ,  B01J2219/00274 ,  B01J2219/00315 ,  B01J2219/00317 ,  B01J2219/00585 ,  B01J2219/00599 ,  B01J2219/00707 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/00731 ,  B01J2219/00736 ,  B01J2219/00743 ,  B01J2219/00747 ,  B01J2219/00752 ,  G01N30/466 ,  G01N30/88 ,  G01N30/89 ,  G01N33/5085 ,  G01N33/558

摘要： Methods and apparatus for the evaluation of interactions between substances using inverse chromatography are disclosed. Preferably, interactions are evaluated between a liquid test sample and a solid phase comprising a receptor in the presence of a liquid carrier. Preferably, one of the modifying agent or receptor are members of a combinatorial library.

摘要翻译： 公开了使用反相色谱法评估物质之间相互作用的方法和装置。 优选地，在液体载体的存在下，在液体测试样品和包含受体的固相之间评估相互作用。 优选地，修饰剂或受体之一是组合文库的成员。

公开(公告)号：US20030027215A1

公开(公告)日：2003-02-06

申请号：US10179573

申请日：2002-06-24

发明人： Miroslav Petro ,  Ralph B. Nielsen ,  Jacqueline M. Regan

IPC分类号： G01N033/53 ,  G01N033/558 ,  G01N033/543

CPC分类号： C40B30/04 ,  B01J2219/00274 ,  B01J2219/00315 ,  B01J2219/00317 ,  B01J2219/00585 ,  B01J2219/00599 ,  B01J2219/00707 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/00731 ,  B01J2219/00736 ,  B01J2219/00743 ,  B01J2219/00747 ,  B01J2219/00752 ,  G01N30/466 ,  G01N30/88 ,  G01N30/89 ,  G01N33/5085 ,  G01N33/558

摘要： Methods and apparatus for the evaluation of interactions between substances using inverse chromatography are disclosed. Preferably, interactions are evaluated between a liquid test sample and a solid phase comprising a receptor in the presence of a liquid carrier. Preferably, one of the modifying agent or receptor are members of a combinatorial library.

摘要翻译： 公开了使用反相色谱法评估物质之间相互作用的方法和装置。 优选地，在液体载体的存在下，在液体测试样品和包含受体的固相之间评估相互作用。 优选地，修饰剂或受体之一是组合文库的成员。

公开(公告)号：US06490910B1

公开(公告)日：2002-12-10

申请号：US09830089

申请日：2001-04-23

申请人： David A. Butler ,  Carl Levoguer ,  Daryl R. Williams

发明人： David A. Butler ,  Carl Levoguer ,  Daryl R. Williams

IPC分类号： G01N2502

CPC分类号： G01N30/466 ,  G01N30/34 ,  G01N30/89 ,  G01N2030/025 ,  G01N2030/626

摘要： Inverse gas-chromatography apparatus for investigating the properties of a solid material, having a carrier fluid, comprising at least two fluid components (typically water and an inert gas); a second flow comprising the carrier fluid components and probe material; a temperature controlled column, for holding the solid material; a switch for selectively passing the flow of carrier fluid and the second fluid flow through the column; a detector for detecting the passage of the probe material through the column. The relative proportions of the fluid components in the flow of carrier fluid and the second fluid flow can be controlled.

摘要翻译： 用于研究具有载体流体的固体材料的性质的反相气相色谱装置，其包含至少两种流体组分（通常为水和惰性气体）; 包括载体流体组分和探针材料的第二流; 用于保持固体材料的温度控制柱; 用于选择性地使载体流体的流动和第二流体流通过柱的开关; 用于检测探针材料通过柱的通过的检测器。 可以控制流体组分在载体流体和第二流体流中的相对比例。

公开(公告)号：US20020125194A1

公开(公告)日：2002-09-12

申请号：US10142785

申请日：2002-05-13

发明人： Irving Wainer ,  Yanxiao Zhang

IPC分类号： B01D015/08

CPC分类号： B01D15/3804 ,  B01J20/28033 ,  B01J20/286 ,  B01J20/3204 ,  B01J20/3242 ,  B01J20/3246 ,  B01J20/3251 ,  B01J20/3274 ,  B01J2220/54 ,  B01J2220/58 ,  G01N30/02 ,  G01N30/48 ,  G01N30/7233 ,  G01N30/89 ,  G01N2030/527

摘要： This invention provides a immobilized receptors on supports in liquid chromatographic systems. The method of the invention provides means of evaluating the attachment of agents to receptors comprising the steps of: (a) immobilizing receptors on artificial membrane supports in a column, (b) exposing the supports with the receptors to test agents at varying concentrations in a liquid chromatographic system, (c) eluting the test agent from the column, and (d) evaluating the elution profile of the test materials from the column. Using this method, it is possible to evaluate the interaction of the test agent with the receptor. Following elution, it is possible to directly determine molecular structure by passing the elute through other testing devices such as a mass spectrometer.

摘要翻译： 本发明在液相色谱系统中提供载体上的固定化受体。 本发明的方法提供了评估试剂与受体的连接的方法，包括以下步骤：（a）将受体固定在柱上的人造膜支持物上，（b）将载体与受体暴露于不同浓度的试剂 液相色谱系统，（c）从柱中洗脱测试试剂，和（d）从柱中评估测试材料的洗脱曲线。 使用该方法，可以评估测试剂与受体的相互作用。 洗脱后，可以通过将洗脱液通过其它测试装置如质谱仪来直接确定分子结构。

公开(公告)号：US6435012B2

公开(公告)日：2002-08-20

申请号：US92408401

申请日：2001-08-07

申请人： ROHM & HAAS

发明人： MAIKNER JOHN JOSEPH

IPC分类号： G01N30/00 ,  B01D15/08 ,  B01D15/16 ,  B01D15/20 ,  B01D15/32 ,  B01D15/34 ,  B01J20/26 ,  B01J20/28 ,  B01J20/281 ,  B01J20/285 ,  G01N30/88 ,  G01N30/89 ,  G01N30/90

CPC分类号： G01N30/482 ,  B01D15/166 ,  B01D15/20 ,  B01D15/325 ,  B01D15/327 ,  B01D15/34 ,  B01J20/26 ,  B01J20/285 ,  B01J2220/54 ,  G01N30/02 ,  G01N30/89

摘要： An improved method for measuring the chromatographic properties, such as excluded volume, of hydrophobic polymer substrates is disclosed. Use of 0.05-1 micron sized crosslinked ionically-charged polymer particles, especially emulsion-form or ground polymer particles, as a large-molecule probe, allows environmentally friendly aqueous-based solvent systems to be used as mobile phases to characterize hydrophobic polymer supports for use in analytical or preparative scale chromatographic applications. This method eliminates the use of non-polar organic solvents that is required when conventional non-polar large-molecule probes (such as polystyrene) are used to characterize chromatographic media.