公开(公告)号：US20180208996A1

公开(公告)日：2018-07-26

申请号：US15923954

申请日：2018-03-16

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Caifu CHEN ,  Ruoying TAN

IPC: C12Q1/6886 ,  C12Q1/6858

CPC classification number: C12Q1/6886 ,  C07H21/04 ,  C12Q1/6858 ,  C12Q2600/156 ,  C12Q2600/172 ,  C12Q2561/113 ,  C12Q2561/101 ,  C12Q2537/161

Abstract: In some embodiments, the present inventions relates generally to compositions, methods and kits for use in discriminating sequence variation between different alleles. More specifically, in some embodiments, the present invention provides for compositions, methods and kits for quantitating rare (e.g., mutant) allelic variants, such as SNPs, or nucleotide (NT) insertions or deletions, in samples comprising abundant (e.g., wild type) allelic variants with high specificity and selectivity. In particular, in some embodiments, the invention relates to a highly selective method for mutation detection referred to as competitive allele-specific TaqMan PCR (“cast-PCR”).

公开(公告)号：US20180200374A1

公开(公告)日：2018-07-19

申请号：US15844380

申请日：2017-12-15

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Zhiwei YANG ,  Parul ANGRISH ,  Xavier de MOLLERAT du JEU ,  Kristin WIEDERHOLT

IPC: A61K47/54 ,  C07D211/58 ,  A61K48/00 ,  C12N15/88 ,  C12N15/113 ,  C07D211/28 ,  C07C229/08 ,  C07C227/06

CPC classification number: A61K47/54 ,  A61K47/543 ,  A61K48/0008 ,  C07C227/06 ,  C07C229/08 ,  C07D211/28 ,  C07D211/58 ,  C12N15/1137 ,  C12N15/88 ,  C12N2310/11 ,  C12N2310/141 ,  C07C229/26

Abstract: There are provided for herein novel amine-containing transfection compounds and methods for making and using same. The compounds are generally obtained by reacting a primary amine with an unsaturated compound. Transfection complexes made using the amine-containing transfection compounds in combination with additional compounds to encapsulate biologically active agents such as nucleic acids are also provided for herein. Methods of using the transfection complexes for the in vivo or in vitro delivery of biologically active agents are also described. The transfection complexes of the present invention are highly potent, thereby allowing effective modulation of a biological activity at relatively low doses compared to analogous transfection compounds known in the art.

公开(公告)号：US20180195089A1

公开(公告)日：2018-07-12

申请号：US15842664

申请日：2017-12-14

Applicant: LIFE TECHNOLOGIES CORPORATION ,  THERMO FISHER SCIENTIFIC GENEART GMBH

Inventor： Namritha RAVINDER ,  Korbinian HEIL ,  Yizhu GUO ,  Xiquan LIANG ,  Robert POTTER ,  Sanjay KUMAR

IPC: C12N15/90 ,  C12N15/10 ,  C12Q1/686 ,  C12P19/34 ,  C12N15/11

CPC classification number: C12N15/907 ,  C12N15/102 ,  C12N15/11 ,  C12N2310/3519 ,  C12P19/34 ,  C12Q1/686 ,  C12Q2525/143

Abstract: The present disclosure generally relates to compositions and methods for the genetic modification of cells. In particular, the disclosure relates to CRISPR reagents and the use of such reagents.

公开(公告)号：US20180195034A1

公开(公告)日：2018-07-12

申请号：US15916635

申请日：2018-03-09

Applicant: Life Technologies Corporation

Inventor： Christopher D. Brau ,  Nephi D. Jones ,  Benjamin R. Madsen ,  Michael E. Goodwin

IPC: C12M1/00 ,  C12M1/34 ,  B01F3/04 ,  C12M1/06 ,  C12M1/02 ,  B01F7/22 ,  B01F13/02 ,  B01F15/00 ,  B01F11/00

Abstract: A system for performing a gas-liquid mass transfer includes a container bounding a compartment and having a top wall, a bottom wall, and an encircling sidewall extending therebetween. A tube has a first end and an opposing second end, the first end of the tube being disposed within the compartment of the container. A nozzle is disposed within the compartment of the container and has at least one outlet, the nozzle being coupled with the tube so that a gas can be passed through the tube and out the at least one outlet of the nozzle. The nozzle is sufficiently buoyant so that when a fluid is disposed within the compartment of the container, the nozzle floats on the fluid.

公开(公告)号：USD822847S1

公开(公告)日：2018-07-10

申请号：US29585174

申请日：2016-11-21

Applicant: LIFE TECHNOLOGIES CORPORATION

Designer： Kuan Moon Boo ,  Way Xuang Lee ,  Wuh Ken Loh ,  Zeqi Tan ,  Sandro Klein

公开(公告)号：USD822052S1

公开(公告)日：2018-07-03

申请号：US29618478

申请日：2017-09-21

Applicant: Life Technologies Corporation

Designer： Scott Rickes ,  Laurel Stone ,  Kyle Curts ,  George Hanson ,  Adam Zahner ,  Lubasha Chabansky ,  Zenia Wei

公开(公告)号：US10005908B2

公开(公告)日：2018-06-26

申请号：US15194911

申请日：2016-06-28

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jason Dallwig ,  David Hagen ,  Ching-Ying Cheung ,  Gerald Thomas ,  Stephen Yue

IPC: C07D277/64 ,  C07D417/06 ,  C09B23/16 ,  C09B23/01 ,  C09B23/06 ,  G01N33/53 ,  C09B23/04 ,  C09B23/10 ,  C12Q1/68

CPC classification number: C09B23/164 ,  C07D277/64 ,  C07D417/06 ,  C09B23/0008 ,  C09B23/04 ,  C09B23/06 ,  C09B23/107 ,  C12Q1/6806 ,  C12Q1/6876 ,  C12Q2600/16 ,  G01N33/5308 ,  Y10T436/143333

Abstract: Cyanine dye compounds having a substituted methine moiety that are nucleic acid stains, particularly for fluorescent staining of RNA, including compounds having the formula where R1 is a C1-C6 alkyl, sulfoalkyl, carboxyalkyl or C1-C6 alkoxy; each R2 is independently selected from the group consisting of H, C1-C6 alkyl, C1-C6 alkoxy, fused benzo, trifluoromethyl, amino, sulfo, carboxy and halogen, that is optionally further substituted; at least one of R3, R4, and R5 is an alkyl, aryl, heteroaryl, cyclic, or heterocyclic moiety that is optionally substituted by alkyl, amino, aminoalkyl, carboxy, nitro, or halogen; and the remaining R3, R4 or R5 are hydrogen; X is S, O, or Se; and D is a substituted or unsubstituted pyridinium, quinolinium or benzazolium moiety.

公开(公告)号：US20180165410A1

公开(公告)日：2018-06-14

申请号：US15834520

申请日：2017-12-07

Applicant: Life Technologies Corporation

Inventor： Ruchi Chaudhary ,  Fiona Hyland

IPC: G06F19/18

Abstract: A targeted panel with low sample input requirements from a tumor only sample may be processed to estimate mutation load in a tumor sample. The method, including detecting variants in nucleic acid sequence reads corresponding to targeted locations in the tumor sample genome; annotating detected variants with an annotation information from a population database; filtering the detected variants, wherein the filtering rule set retains the somatic variants and removes germ-line variants; counting the identified somatic variants to give a number of somatic variants; determining a number of bases in covered regions of the targeted locations in the tumor sample genome; and calculating a number of somatic variants per megabase, provides an estimate of the mutation load per megabase in the tumor sample genome.

公开(公告)号：US09995708B2

公开(公告)日：2018-06-12

申请号：US14543551

申请日：2014-11-17

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Keith G. Fife ,  James Bustillo ,  Jordan Owens

IPC: G01N27/414

CPC classification number: G01N27/414 ,  G01N27/4145

Abstract: In one implementation, a chemical sensor is described. The chemical sensor includes chemically-sensitive field effect transistor including a floating gate conductor having an upper surface. A dielectric material defines an opening extending to the upper surface of the floating gate conductor. A conductive sidewall spacer is on a sidewall of the opening and contacts the upper surface of the floating gate conductor.

公开(公告)号：US20180149847A1

公开(公告)日：2018-05-31

申请号：US15825895

申请日：2017-11-29

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Steven LYTLE ,  Paul BOESCHOTEN ,  Andrew GUNDERSON ,  Chris GNEHM

IPC: G02B21/00 ,  G02B21/36

CPC classification number: G02B21/0032 ,  G02B21/0044 ,  G02B21/0056 ,  G02B21/0076 ,  G02B21/362 ,  G02B27/141

Abstract: An apparatus for generating structured illumination images, comprising a housing; imaging optics mounted within the housing, the imaging optics configured to focus illumination from an illumination source such that an in-focus pattern of light corresponding to a pattern on a photomask is projected onto a sample; a reflector mounted within the housing and configured to reflect the illumination to a sample; a filter apparatus, comprising one or more filters mounted within the housing, the filter apparatus configured to allow only emissions from the illuminated sample to pass through to the sensor; and mounting features on the housing, the mounting features configured to allow the apparatus to be installed within an imaging system.