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    METHOD AND APPARATUS FOR PURIFYING NUCLEIC ACID ON HYDROPHILIC SURFACE OF SOLID SUPPORT USING HYDROGEN BONDING
    32.
    发明申请
    METHOD AND APPARATUS FOR PURIFYING NUCLEIC ACID ON HYDROPHILIC SURFACE OF SOLID SUPPORT USING HYDROGEN BONDING 审中-公开
    使用氢键连接固体支持的亲水表面上的核酸的方法和装置

    公开(公告)号：US20070238109A1

    公开(公告)日：2007-10-11

    申请号：US11553657

    申请日：2006-10-27

    Applicant: Jun-hong MIN ,  In-ho LEE ,  Chang-eun YOO ,  Ki-woong HAN ,  Young-rok KIM

    Inventor： Jun-hong MIN ,  In-ho LEE ,  Chang-eun YOO ,  Ki-woong HAN ,  Young-rok KIM

    IPC: C12Q1/68 ,  C12M3/00

    CPC classification number: C12N15/1006 ,  C12N15/1003

    Abstract: Provided is a method of purifying nucleic acid, the method including: contacting a nucleic acid-containing sample and a solution containing a kosmotropic salt on a solid support having a hydrophilic functional group on its surface to bind the nucleic acid to the solid support. Since the solid support is used as it is without any surface treatment, manufacture of the apparatus is very easy, and nucleic acid can be bound to the solid support without specific additives in a wide pH range, so that the apparatus can be used for a Lab-On-a-Chip.

    Abstract translation: 提供了一种纯化核酸的方法，所述方法包括：使含核酸的样品和含有可变色盐的溶液在其表面上具有亲水性官能团的固体支持物上接触，以将核酸结合到固体支持物上。 由于固体支持物原样使用而没有进行任何表面处理，所以装置的制造非常容易，并且核酸可以在宽的pH范围内结合固体支持物而没有特定的添加剂，使得该装置可以用于 实验室芯片

    pH dependent ion exchange material capable of selectively binding to nucleic acids in comparison with proteins, solid substrate having the material immobilized on its surface, and method of isolating a nucleic acid using the material or the solid substrate
    33.
    发明申请
    pH dependent ion exchange material capable of selectively binding to nucleic acids in comparison with proteins, solid substrate having the material immobilized on its surface, and method of isolating a nucleic acid using the material or the solid substrate 有权
    与蛋白质相比能够选择性地结合核酸的pH依赖性离子交换材料，具有固定在其表面上的材料的固体底物，以及使用该材料或固体基质分离核酸的方法

    公开(公告)号：US20060264621A1

    公开(公告)日：2006-11-23

    申请号：US11437210

    申请日：2006-05-19

    Applicant: Kyu-youn Hwang ,  Chang-eun Yoo

    Inventor： Kyu-youn Hwang ,  Chang-eun Yoo

    IPC: C07H21/04 ,  C07H21/00 ,  B01J41/12

    CPC classification number: B01J43/00 ,  B01J39/17 ,  B01J39/20

    Abstract: Provided is a pH dependent ion exchange material. The pH dependent ion exchange material has at least one monomer selected from the group consisting of M0, M1, M2 and M3 represented by the following formulae, provided that at least one monomer having A and at least one monomer having B are contained therein, the pH dependent ion exchange material having a degree of polymerization of 2-30,000. wherein A is a group —X(CH2)nY, wherein n is an integer from 1 to 10, X is a functional group which can react with an activated ester, and Y is a primary, secondary, tertiary amine or a nitrogen-containing aromatic heterocyclic base, B is a group —X′(CH2)nY′, wherein n is an integer from 1 to 10, X′ is a functional group which can react with an activated ester, and Y′ is an acid generating group having a pKa value of 4 or less, and R3 is an alkyl group having 1 to 10 carbon atoms.

    Abstract translation: 提供了一种依赖于pH的离子交换材料。 pH依赖性离子交换材料具有至少一种选自由下式表示的M0，M1，M2和M3的单体，条件是含有至少一种具有A和至少一种具有B的单体的单体， 具有2-30,000聚合度的pH依赖性离子交换材料。 其中A为基团-X（CH 2 CH 2）n Y，其中n为1至10的整数，X为可与活化酯反应的官能团 ，Y是伯，仲，叔胺或含氮芳族杂环碱，B是基团-X'（CH 2）n Y'，其中 n为1〜10的整数，X'为能够与活化酯反应的官能团，Y'为pK a> 4以下的产酸基，R' 3是具有1至10个碳原子的烷基。

    Microarray holding device
    34.
    发明申请
    Microarray holding device 审中-公开
    微阵列保持装置

    公开(公告)号：US20050271557A1

    公开(公告)日：2005-12-08

    申请号：US11106209

    申请日：2005-04-14

    Applicant: Hun-joo Lee ,  Nam Huh ,  Sang-hyun Paek ,  Chang-eun Yoo

    Inventor： Hun-joo Lee ,  Nam Huh ,  Sang-hyun Paek ,  Chang-eun Yoo

    IPC: G01N33/52 ,  B01L9/00

    CPC classification number: B01L9/52 ,  B01J2219/00608 ,  B01J2219/00621 ,  B01J2219/00659 ,  B01J2219/00722 ,  B01L2300/0819

    Abstract: Provided is a microarray holding device. The microarray holding device includes: a solid substrate in the form of a plate in which an engaging-receiving portion for receiving a microarray substrate to be engaged therewith is formed, the engaging-receiving portion being composed of an opening; sidewalls to be aligned with sidewalls of the microarray substrate to be engaged with the engaging-receiving portion; and a base surface on which a top or bottom surface of the microarray substrate to be engaged with the engaging-receiving portion is to be placed, wherein the base surface is depressed from a top or bottom surface of the solid substrate such that the other surface of the microarray substrate is lower than the top or bottom surface of the solid substrate when the microarray substrate is engaged with the engaging-receiving portion.

    Abstract translation: 提供了一种微阵列保持装置。 微阵列保持装置包括：形成板状的固体基板，其中形成用于接收与其接合的微阵列基板的接合部分，所述接合部分由开口构成; 与微阵列基板的侧壁对准以与接合部接合的侧壁; 以及要在其上放置要与所述接合部分接合的所述微阵列基板的顶表面或底表面的基面，其中所述基底表面从所述固体基底的顶表面或底表面压下，使得所述另一表面 的微阵列基板与接合部接合时，低于固体基板的顶面或底面。

    Method and apparatus for concentrating and amplifying nucleic acid in single micro chamber
    36.
    发明授权
    Method and apparatus for concentrating and amplifying nucleic acid in single micro chamber 有权
    在单微室中浓缩和扩增核酸的方法和装置

    公开(公告)号：US07807360B2

    公开(公告)日：2010-10-05

    申请号：US11620961

    申请日：2007-01-08

    Applicant: Young-rok Kim ,  Jun-hong Min ,  In-ho Lee ,  Young-sun Lee ,  Chang-eun Yoo ,  Ki-woong Han

    Inventor： Young-rok Kim ,  Jun-hong Min ,  In-ho Lee ,  Young-sun Lee ,  Chang-eun Yoo ,  Ki-woong Han

    IPC: C12Q1/68

    CPC classification number: C12Q1/6806 ,  B01L3/5027 ,  B01L3/5088 ,  C12Q1/686 ,  C12Q2565/629 ,  C12Q2537/149 ,  C12Q2527/125

    Abstract: A method of sequentially performing concentration and amplification of nucleic acid in a single micro chamber includes: introducing a nucleic acid-containing sample and a solution including a kosmotropic salt to a micro chamber having a hydrophilic interior surface to concentrate the nucleic acid by binding the nucleic acid on the interior surface of the micro chamber; and performing a polymerase chain reaction (PCR) by adding a PCR mixture to the chamber. Since the nucleic acid is reversibly bound to the interior surface of the micro chamber, PCR yield is higher compared with a surface of aluminum oxide in which irreversible binding occurs. In addition, all processes are sequentially performed in a single micro chamber so that the number of samples, consumables, time, and labor for treatment and analysis can be reduced, detection sensitivity can be improved, and risk of sample cross contamination significantly reduced without sample loss by eliminating transporting of the sample. A complete automated system for concentration and amplification of nucleic acid is thus readily provided.

    Abstract translation: 在单个微室中依次进行核酸的浓缩和扩增的方法包括：将含核酸的样品和包含可染色盐的溶液引入具有亲水性内表面的微室中，以通过结合核酸来浓缩核酸 酸在微室的内表面上; 并通过向该室加入PCR混合物进行聚合酶链式反应（PCR）。 由于核酸可逆地结合到微室的内表面，与其中发生不可逆结合的氧化铝的表面相比，PCR产率更高。 此外，所有过程都在单个微室中顺序进行，以便可以减少样品数量，消耗品，时间和处理和分析的劳动力，可以提高检测灵敏度，并且样品交叉污染的风险在没有样品时显着降低 通过消除样品的运输而损失。 因此容易提供用于浓缩和扩增核酸的完整的自动化系统。

    Cell lysis method by immobilized metal-ligand complex
    39.
    发明授权
    Cell lysis method by immobilized metal-ligand complex 失效
    固定化金属配体复合物的细胞裂解法

    公开(公告)号：US07648838B2

    公开(公告)日：2010-01-19

    申请号：US11267005

    申请日：2005-11-04

    Applicant: Chang-eun Yoo ,  Joon-ho Kim ,  Kyu-youn Hwang ,  Hun-joo Lee ,  Hee-kyun Lim ,  Soo-min Ma ,  Nam Huh ,  Soo-suk Lee

    Inventor： Chang-eun Yoo ,  Joon-ho Kim ,  Kyu-youn Hwang ,  Hun-joo Lee ,  Hee-kyun Lim ,  Soo-min Ma ,  Nam Huh ,  Soo-suk Lee

    IPC: G01N33/48 ,  G01N33/20 ,  G01N1/00

    CPC classification number: C12Q1/6806 ,  Y10T436/25 ,  C12Q2523/308

    Abstract: Provided is a lysis method for cells or viruses, including: immobilizing a metal-ligand complex on a solid support; and mixing the complex immobilized on the support with a cell or virus solution. According to the lysis method, by immobilizing a chemical on a solid support to perform cell lysis, the dilution problem according to the addition of a cell lysis solution can be resolved and a separate process of removing the chemical is not required so as to reduce the steps upon LOC implementation. In addition, since a variety of solid supports, such as chips, beads, nanoparticles etc. can be used, cell lysis apparatuses of various forms can be fabricated.

    Abstract translation: 提供了一种细胞或病毒的裂解方法，包括：将固定支持物上的金属 - 配体复合物固定; 并将固定在载体上的复合物与细胞或病毒溶液混合。 根据裂解方法，通过将化学物质固定在固体支持体上以进行细胞裂解，可以解决根据细胞溶解溶液的添加的稀释问题，并且不需要单独的除去化学品的方法，以减少 LOC实施步骤。 此外，由于可以使用各种固体支持物，例如芯片，珠粒，纳米粒子等，因此可以制造各种形式的细胞裂解装置。

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