公开(公告)号：US10684281B2

公开(公告)日：2020-06-16

申请号：US15970702

申请日：2018-05-03

申请人： Illumina Cambridge Limited

发明人： Xavier von Hatten ,  Wayne N. George ,  Alexandre Richez ,  Anne-Cecile Dingwall ,  Andrew A. Brown

IPC分类号： G01N33/543 ,  C12Q1/6834 ,  C12Q1/6869 ,  G01N33/551 ,  C12N11/10 ,  C09D183/04

摘要： Some embodiments described herein relate to a substrate comprising a silane functionalized surface for reversibly immobilizing a biological molecule of interest, such as oligonucleotides, polynucleotides, or protein. Methods for immobilizing the biological molecule and the use in DNA sequencing and other diagnostic applications are also disclosed.

公开(公告)号：US20200157619A1

公开(公告)日：2020-05-21

申请号：US16657931

申请日：2019-10-18

申请人： Illumina Cambridge Limited

发明人： Wouter Meuleman

IPC分类号： C12Q1/6869

摘要： A method for determining the sequence of a target nucleic acid, including steps of contacting a target nucleic acid with a polymerase to sequentially remove nucleotide triphosphates from the target nucleic acid, wherein the nucleotide triphosphates that are removed have a variety of different base moieties; and distinguishing the different base moieties for the nucleotide triphosphates that are removed. Also provided is a apparatus including a nanopore positioned in a fluid impermeable barrier to form a passage through which a nucleotide triphosphate can pass from a first fluid reservoir to a second fluid reservoir, and a reaction mix in the first fluid reservoir that includes a polymerase, target nucleic acid having two strands, and pyrophosphorolytic concentration of pyrophosphate.

公开(公告)号：US20200080142A1

公开(公告)日：2020-03-12

申请号：US16468269

申请日：2018-03-06

申请人： ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

发明人： Robert LANGLOIS ,  John VIECELI ,  Xiaohai LIU

IPC分类号： C12Q1/6869 ,  G01N21/64

摘要： Disclosed is a system for determining the nucleotide sequence of polynucleotides. The system can comprise a light source, such as a laser or a LED, configured to generate light at a predetermined wavelength. A detector of the system can detect fluorescent emissions at a first wavelength and a second wavelength. A processor of the system identify the nucleotide as a first type if no fluorescent emission is detected by the at least one detector; identify the nucleotide as a second type if a fluorescent emission at the first wavelength of light is detected by the at least one detector; identify the nucleotide as a third type if a fluorescent emission at the second wavelength of light is detected by the at least one detector; and identify the nucleotide as a fourth type if fluorescent emissions at the first wavelength and the second wavelength of light are detected by the at least one detector.

公开(公告)号：US20200041401A1

公开(公告)日：2020-02-06

申请号：US16522345

申请日：2019-07-25

申请人： ILLUMINA CAMBRIDGE LIMITED

发明人： Xavier von Hatten

IPC分类号： G01N15/14 ,  C12Q1/6869

摘要： In an example, a flow cell includes a substrate, a selectively removable porous molecular network on the substrate and defining exposed substrate regions, and sequencing surface chemistry on at least some of the exposed regions. The sequencing surface chemistry is selected from the group consisting of i) an activated pad, a polymer layer attached to the activated pad, and a primer attached to the polymer layer; or ii) a nanostructure and an enzyme attached to the nanostructure.

公开(公告)号：US20200002747A1

公开(公告)日：2020-01-02

申请号：US16561483

申请日：2019-09-05

申请人： Illumina Cambridge Limited

发明人： Roberto Rigatti ,  Niall Anthony Gormley

IPC分类号： C12Q1/6806 ,  C12Q1/6874

摘要： Embodiments provided herein relate to methods and compositions for next generation sequencing. Some embodiments include the preparation of a template library from a target nucleic acid in contact with a surface, and sequencing the library on the surface

公开(公告)号：US10519496B2

公开(公告)日：2019-12-31

申请号：US15470801

申请日：2017-03-27

申请人： Illumina Cambridge Limited

发明人： Shankar Balasubramanian ,  Colin Lloyd Barnes ,  Xiaohai Liu ,  Xiaolin Wu ,  John Milton

IPC分类号： C12Q1/68 ,  C07H19/10 ,  C12Q1/6869 ,  C07H19/20 ,  C07H21/00 ,  C12P19/34 ,  C12Q1/6876 ,  C12Q1/6874 ,  C12Q1/6818 ,  C12Q1/6834 ,  C12Q1/6806 ,  C12Q1/6823

摘要： Nucleosides and nucleotides are disclosed that are linked to detectable labels via a cleavable linker group.

公开(公告)号：US20190352327A1

公开(公告)日：2019-11-21

申请号：US16411913

申请日：2019-05-14

申请人： Illumina, Inc. ,  Illumina Cambridge Limited

发明人： Xiaolin Wu ,  Randall Smith ,  Peyton Shieh ,  John M. Beierle ,  Wayne N. George ,  Elliot John Lawrence ,  Jie Mao ,  Xiaohai Liu

IPC分类号： C07H21/04 ,  C12Q1/6876

摘要： Embodiments of the present disclosure relate to methods of preparation of templates for polynucleotide sequencing. In particular, the disclosure relates to linearization of clustered polynucleotides in preparation for sequencing by cleavage of one or more first strands of double-stranded polynucleotides immobilized on a solid support by a transition metal complex, for example, a palladium complex or a nickel complex. Further disclosure relate to linearization of clustered polynucleotides by cleaving one or more second strands of double double-stranded polynucleotides immobilized on a solid support comprising azobenzene linker by Na2S2O4. Nucleotides and oligonucleotides comprising a 3′ phosphate moiety blocking group, and methods of removing the same using a fluoride reagent are also disclosed.

公开(公告)号：US10450605B2

公开(公告)日：2019-10-22

申请号：US15847362

申请日：2017-12-19

申请人： Illumina Cambridge Limited

发明人： Wouter Meuleman

IPC分类号： C12P19/34 ,  C12Q1/6869

摘要： A method for determining the sequence of a target nucleic acid, including steps of contacting a target nucleic acid with a polymerase to sequentially remove nucleotide triphosphates from the target nucleic acid, wherein the nucleotide triphosphates that are removed have a variety of different base moieties; and distinguishing the different base moieties for the nucleotide triphosphates that are removed. Also provided is a apparatus including a nanopore positioned in a fluid impermeable barrier to form a passage through which a nucleotide triphosphate can pass from a first fluid reservoir to a second fluid reservoir, and a reaction mix in the first fluid reservoir that includes a polymerase, target nucleic acid having two strands, and pyrophosphorolytic concentration of pyrophosphate.

公开(公告)号：US20190224676A1

公开(公告)日：2019-07-25

申请号：US16244776

申请日：2019-01-10

申请人： Illumina, Inc. ,  Illumina Cambridge Limited

发明人： Wesley A. COX-MURANAMI ,  Darren Robert SEGALE ,  Oliver Jon MILLER ,  Jennifer Olivia FOLEY ,  Tarun Kumar KHURANA ,  Paul CRIVELLI ,  Gary WATTS

IPC分类号： B01L3/00 ,  G01N1/31

摘要： An apparatus for directing fluid into and out of a fluidic device includes two or more fluid prime channels connected to a fluid inlet of the fluidic device, a flow control valve for each fluid prime channel to control flow between the fluid prime channel and the fluid inlet, one or more outlet channels connected to a fluid outlet of the fluidic device, and a flow control valve for each outlet channel to control flow between the fluid outlet and the associated outlet channel. An apparatus for delivering fluids to a fluid inlet includes a plate that is rotatable about an axis of rotation and a plurality of fluid compartments disposed on the plate, each compartment having a fluid exit port disposed at a common radial distance from the axis of rotation and positioned to align with the fluid inlet as the plate rotates about the axis of rotation.

公开(公告)号：US10329613B2

公开(公告)日：2019-06-25

申请号：US14683580

申请日：2015-04-10

申请人： Illumina Cambridge Limited

发明人： Roberto Rigatti ,  Niall Anthony Gormley ,  Jonathan Mark Boutell

IPC分类号： C12P19/34 ,  C12Q1/6874

摘要： Provided herein is a method for sequencing a polynucleotide molecules. The method includes the steps of providing a plurality of polynucleotide molecules attached to a surface, wherein a first portion of each polynucleotide molecule is attached to a first location of the surface and a second portion of each polynucleotide molecule is attached to a second location of the surface, the relative proximity of the first and second locations being correlated with the probability that the first and second portions are paired, separating the first and second portions of the polynucleotide molecules on the surface, determining the sequences of the first and second portions of the polynucleotide molecules and comparing the relative proximities and the sequences to determine which first and second portions are paired and to determine the sequence of the target polynucleotide molecules.