公开(公告)号：US20200225129A1

公开(公告)日：2020-07-16

申请号：US16733137

申请日：2020-01-02

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Benjamin Miller ,  Brian Hutchison ,  Andrew Wilson ,  Jonathan William Larson ,  Qun Zhong ,  Yevgeny Yurkovetsky ,  Darren Roy Link ,  Mark Weary

IPC: G01N1/38 ,  B01L3/02 ,  B01L3/00 ,  B01F15/00 ,  B01F13/00 ,  F17D3/01 ,  G01N27/447 ,  F16K99/00

Abstract: The invention provides methods for assessing one or more predetermined characteristics or properties of a microfluidic droplet within a microfluidic channel, and regulating one or more fluid flow rates within that channel to selectively alter the predetermined microdroplet characteristic or property using a feedback control.

公开(公告)号：US20190134581A1

公开(公告)日：2019-05-09

申请号：US16181256

申请日：2018-11-05

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Yevgeny Yurkovetsky ,  Darren Roy Link ,  Jonathan William Larson

IPC: B01F13/00 ,  B01L7/00 ,  B01L3/00 ,  B01F3/08 ,  B01F5/00 ,  B01F5/04

Abstract: The invention generally relates to methods for forming mixed droplets. In certain embodiments, methods of the invention involve forming a droplet, and contacting the droplet with a fluid stream, wherein a portion of the fluid stream integrates with the droplet to form a mixed droplet.

公开(公告)号：US12031175B2

公开(公告)日：2024-07-09

申请号：US18514645

申请日：2023-11-20

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Jonathan William Larson ,  Qun Zhong ,  Darren Roy Link

IPC: C12Q1/68 ,  C12Q1/6816 ,  C12Q1/6827 ,  C12Q1/686 ,  C12Q1/6883 ,  C12Q1/6886

CPC classification number: C12Q1/6816 ,  C12Q1/6827 ,  C12Q1/686 ,  C12Q1/6883 ,  C12Q1/6886 ,  C12Q2600/172 ,  C12Q1/686 ,  C12Q2563/159 ,  C12Q2565/629 ,  C12Q1/686 ,  C12Q2537/143 ,  C12Q2563/159 ,  C12Q2565/629 ,  C12Q1/6827 ,  C12Q2531/113 ,  C12Q2537/143 ,  C12Q2563/159 ,  C12Q2565/629 ,  C12Q1/686 ,  C12Q2535/131 ,  C12Q2537/143 ,  C12Q2563/159 ,  C12Q2565/629 ,  C12Q1/6827 ,  C12Q2531/113 ,  C12Q2535/131 ,  C12Q2537/143 ,  C12Q2563/159 ,  C12Q2565/629

Abstract: The invention generally relates to droplet based digital PCR and methods for analyzing a target nucleic acid using the same. In certain embodiments, methods of the invention involve forming sample droplets containing, on average, a single target nucleic acid, amplifying the target in the droplets, excluding droplets containing amplicon from the target and amplicon from a variant of the target, and analyzing target amplicons.

公开(公告)号：US20240132939A1

公开(公告)日：2024-04-25

申请号：US18536935

申请日：2023-12-12

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Jonathan William Larson ,  Qun Zhong ,  Darren Roy Link

IPC: C12Q1/6816 ,  C12Q1/6827 ,  C12Q1/686

CPC classification number: C12Q1/6816 ,  C12Q1/6827 ,  C12Q1/686 ,  C12Q1/6883

Abstract: The invention generally relates to droplet based digital PCR and methods for analyzing a target nucleic acid using the same. In certain embodiments, methods of the invention involve forming sample droplets containing, on average, a single target nucleic acid, amplifying the target in the droplets, excluding droplets containing amplicon from the target and amplicon from a variant of the target, and analyzing target amplicons.

公开(公告)号：US11946929B2

公开(公告)日：2024-04-02

申请号：US18354144

申请日：2023-07-18

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Michael L. Samuels ,  Darren Roy Link

IPC: G01N33/543 ,  B01F33/3011 ,  B01F33/302 ,  C12Q1/6874 ,  C40B30/04 ,  C40B40/00 ,  C40B40/04 ,  C40B70/00 ,  G01N33/532

CPC classification number: G01N33/54366 ,  C12Q1/6874 ,  C40B40/00 ,  C40B40/04 ,  C40B70/00 ,  G01N33/532 ,  B01F33/3011 ,  B01F33/3021 ,  C40B30/04

Abstract: The invention generally relates to performing sandwich assays in droplets. In certain embodiments, the invention provides methods for detecting a target analyte that involve forming a compartmentalized portion of fluid including a portion of a sample suspected of containing a target analyte and a sample identifier, a first binding agent having a target identifier, and a second binding agent specific to the target analyte under conditions that produce a complex of the first and second binding agents with the target analyte, separating the complexes, and detecting the complexes, thereby detecting the target analyte.

公开(公告)号：US20240002910A1

公开(公告)日：2024-01-04

申请号：US18465623

申请日：2023-09-12

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Jonathan William Larson ,  Qun Zhong ,  Darren Roy Link

IPC: C12Q1/6816 ,  C12Q1/6827 ,  C12Q1/686

CPC classification number: C12Q1/6816 ,  C12Q1/6827 ,  C12Q1/686 ,  C12Q1/6883

Abstract: The invention generally relates to droplet based digital PCR and methods for analyzing a target nucleic acid using the same. In certain embodiments, methods of the invention involve forming sample droplets containing, on average, a single target nucleic acid, amplifying the target in the droplets, excluding droplets containing amplicon from the target and amplicon from a variant of the target, and analyzing target amplicons.

公开(公告)号：US11754499B2

公开(公告)日：2023-09-12

申请号：US16780508

申请日：2020-02-03

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Darren Roy Link ,  Michael L. Samuels

IPC: B01L3/00 ,  G01N21/64 ,  B01J19/00 ,  C40B40/04 ,  C40B50/08 ,  C40B60/10 ,  G01N33/50 ,  C12Q1/6851 ,  B01F23/41 ,  B01F25/433 ,  B01F33/3011 ,  C12Q1/25 ,  C12Q1/6804 ,  C12Q1/6818 ,  C12Q1/6827 ,  G01N33/542 ,  G01N33/573 ,  G01N33/58 ,  B01L7/00

CPC classification number: G01N21/6445 ,  B01F23/41 ,  B01F25/433 ,  B01F25/4336 ,  B01F33/3011 ,  B01J19/0046 ,  B01L3/502761 ,  C12Q1/25 ,  C12Q1/6804 ,  C12Q1/6818 ,  C12Q1/6827 ,  C12Q1/6851 ,  C40B40/04 ,  C40B50/08 ,  C40B60/10 ,  G01N33/5008 ,  G01N33/542 ,  G01N33/573 ,  G01N33/582 ,  B01J2219/0059 ,  B01J2219/0065 ,  B01J2219/0072 ,  B01J2219/0074 ,  B01J2219/00286 ,  B01J2219/00351 ,  B01J2219/00418 ,  B01J2219/00479 ,  B01J2219/00576 ,  B01J2219/00585 ,  B01J2219/00592 ,  B01J2219/00599 ,  B01J2219/00657 ,  B01J2219/00664 ,  B01J2219/00702 ,  B01J2219/00722 ,  B01J2219/00743 ,  B01L7/52 ,  B01L2200/027 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0654 ,  B01L2300/0681 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2400/0415 ,  B01L2400/0487 ,  G01N21/6428 ,  G01N2500/00 ,  C12Q1/6827 ,  C12Q2537/161 ,  C12Q2563/125 ,  C12Q2563/131 ,  C12Q2563/159 ,  C12Q1/6804 ,  C12Q2537/161 ,  C12Q2563/125 ,  C12Q2563/159 ,  C12Q1/6851 ,  C12Q2563/159 ,  C12Q2561/113 ,  C12Q2565/1015

Abstract: The invention generally relates to methods for quantifying an amount of enzyme molecules. Systems and methods of the invention are provided for measuring an amount of target by forming a plurality of fluid partitions, a subset of which include the target, performing an enzyme-catalyzed reaction in the subset, and detecting the number of partitions in the sunset. The amount of target can be determined based on the detected number.

公开(公告)号：US11635427B2

公开(公告)日：2023-04-25

申请号：US16937908

申请日：2020-07-24

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Michael L. Samuels ,  Darren Roy Link

IPC: G01N33/543 ,  C40B40/04 ,  G01N33/532 ,  C40B40/00 ,  C12Q1/6874 ,  B01F33/3011 ,  B01F33/302 ,  C40B70/00

Abstract: The invention generally relates to performing sandwich assays in droplets. In certain embodiments, the invention provides methods for detecting a target analyte that involve forming a compartmentalized portion of fluid including a portion of a sample suspected of containing a target analyte and a sample identifier, a first binding agent having a target identifier, and a second binding agent specific to the target analyte under conditions that produce a complex of the first and second binding agents with the target analyte, separating the complexes, and detecting the complexes, thereby detecting the target analyte.

公开(公告)号：US11596908B2

公开(公告)日：2023-03-07

申请号：US16105042

申请日：2018-08-20

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Darren Roy Link ,  John Brian Hutchison ,  Michael L. Samuels ,  Michael Weiner

IPC: B01F3/08 ,  G01N33/50 ,  C40B50/08 ,  C40B40/04 ,  B01F23/41 ,  B01J19/00 ,  B01L3/00 ,  G01N15/14 ,  B01F25/433 ,  B01F33/3011 ,  B01L7/00 ,  G01N15/10 ,  B01F101/23

Abstract: The present invention generally relates to droplet libraries and to systems and methods for the formation of libraries of droplets. The present invention also relates to methods utilizing these droplet libraries in various biological, chemical, or diagnostic assays.

公开(公告)号：US11511242B2

公开(公告)日：2022-11-29

申请号：US17510092

申请日：2021-10-25

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Darren Roy Link ,  John Brian Hutchison ,  Michael L. Samuels ,  Michael Weiner

IPC: B01F3/08 ,  B01F5/06 ,  B01J19/00 ,  B01F23/41 ,  B01L3/00 ,  C40B40/04 ,  C40B50/08 ,  G01N33/50 ,  G01N15/14 ,  B01F25/433 ,  B01F33/3011 ,  B01L7/00 ,  G01N15/10 ,  B01F101/23

Abstract: The present invention generally relates to droplet libraries and to systems and methods for the formation of libraries of droplets. The present invention also relates to methods utilizing these droplet libraries in various biological, chemical, or diagnostic assays.