-
公开(公告)号：US20240141428A1
公开(公告)日：2024-05-02
申请号：US18489788
申请日：2023-10-18
Applicant: Illumina, Inc.
Inventor： Robert C. Kain , Xiaohai Liu , Wenyi Feng , Bernard Hirschbein , Helmy A. Eltoukhy , Xiaolin Wu , Geoffrey Paul Smith , Jonathan Mark Boutell , Thomas Joseph , Randall Smith , Min-Jui Richard Shen , Carolyn Tregidgo , Kay Klausing
IPC: C12Q1/6874 , C12Q1/6869
CPC classification number: C12Q1/6874 , C12Q1/6869
Abstract: The present disclosure provides methods and systems for detecting multiple different nucleotides in a sample. In particular, the disclosure provides for detection of multiple different nucleotides in a sample utilizing fewer detection moieties than the number of nucleotides being detected and/or fewer imaging events than the number of nucleotides being detected.
-
公开(公告)号：US20230279469A1
公开(公告)日：2023-09-07
申请号：US18146320
申请日：2022-12-23
Applicant: ILLUMINA CAMBRIDGE LIMITED , ILLUMINA, INC. , Illumina Singapore Pte. Ltd.
Inventor： Johan Sebastian Basuki , Jonathan Mark Boutell , Jeffrey S. Fisher , Louise Jane Fraser , Wayne N. George , Niall Anthony Gormley , David Jones , Xiaoyu Ma , Maria Ines Martins Vitoriano , Zhong Mei , Oliver Jon Miller , Andrew Price , Sebastien Georg Gabriel Ricoult , Vicki S. Thomson , Jacqueline C. Weir , Xiaoy Ma , Weihua Chang , Hui Han
IPC: C12Q1/6806 , C12N15/10 , B01L3/00
CPC classification number: C12Q1/6806 , B01L3/502761 , C12N15/1065 , B01L2200/0647 , B01L2300/0893
Abstract: An example of a flow cell includes a substrate having depressions separated by interstitial regions. First and second primers are immobilized within the depressions. First transposome complexes are immobilized within the depressions, and the first transposome complexes include a first amplification domain. Second transposome complexes are also immobilized within the depressions, and the second transposome complexes include a second amplification domain. Some of the first transposome complexes, or some of the second transposome complexes, or some of both of the first and second transposome complexes include a modification to reduce tagmentation efficiency.
-
公开(公告)号：US20230042267A1
公开(公告)日：2023-02-09
申请号：US17855325
申请日：2022-06-30
Applicant: ILLUMINA, INC. , ILLUMINA CAMBRIDGE LIMITED
Inventor： Jonathan Mark Boutell , Wayne N. George , Xiaoyu Ma , Xiaolin Wu , Weixian Xi
IPC: C08G63/685 , C08G63/82 , C08G63/91
Abstract: An example of a kit includes a flow cell, a primer fluid, and a cleaving fluid. The flow cell includes at least one surface functionalized with a polymeric hydrogel including azide functional groups or amine functional groups. The primer fluid includes a plurality of alkyne-containing primers, each alkyne-containing primer having an amino cleavable group attaching a primer sequence of the alkyne-containing primer to an alkyne-containing moiety of the alkyne-containing primer. The cleaving fluid includes a substance that is reactive with the amino cleavable group.
-
公开(公告)号：US20200040386A1
公开(公告)日：2020-02-06
申请号：US16544670
申请日：2019-08-19
Applicant: Illumina, Inc.
Inventor： Min-Jui Richard Shen , Jonathan Mark Boutell , Kathryn M. Stephens , Mostafa Ronaghi , Kevin L. Gunderson , Bala Murali Venkatesan , M. Shane Bowen , Kandaswamy Vijayan
IPC: C12Q1/6848 , B01J19/00 , C12Q1/6844 , C12Q1/6874
Abstract: A method including (a) providing an amplification reagent including an array of sites, and a solution having different target nucleic acids; and (b) reacting the amplification reagent to produce amplification sites each having a clonal population of amplicons from a target nucleic acid from the solution. The reacting can include simultaneously transporting the nucleic acids to the sites at an average transport rate, and amplifying the nucleic acids that transport to the sites at an average amplification rate, wherein the average amplification rate exceeds the average transport rate. The reacting can include producing a first amplicon from a nucleic acid that transports to each of the sites, and producing subsequent amplicons from the nucleic acid or from the first amplicon, wherein the average rate at which the subsequent amplicons are generated exceeds the average rate at which the first amplicon is generated.
-
公开(公告)号：US20190112730A1
公开(公告)日：2019-04-18
申请号：US16158120
申请日：2018-10-11
Applicant: Illumina, Inc.
Inventor： Molly He , Michael Previte , Misha Golynskiy , Matthew William Kellinger , Sergio Peisajovich , Jonathan Mark Boutell
Abstract: A method of characterizing candidate agents including steps of (a) providing a library of candidate agents attached to nucleic acid tags; (b) contacting the library with a solid support to attach the candidate agents to the solid support, whereby an array of candidate agents is formed; (c) contacting the array with a screening agent, wherein one or more candidate agents in the array react with the screening agent; (d) detecting the array to determine that at least one candidate agent in the array reacts with the screening agent; (e) sequencing the nucleic acid tag to determine the tag sequences attached to candidate agents in the array; and (f) identifying the at least one candidate agent in the array that reacts with the screening agent based on the tag sequence that is attached to the at least one candidate agent.
-
Patent Agency Ranking
公开(公告)号：US09758816B2
公开(公告)日：2017-09-12
申请号：US14879932
申请日：2015-10-09
Applicant: Illumina, Inc.
Inventor： Min-Jui Richard Shen , Jonathan Mark Boutell , Kathryn M. Stephens , Mostafa Ronaghi , Kevin Gunderson , Bala Murali Venkatesan , M. Shane Bowen , Kandaswamy Vijayan
CPC classification number: C12Q1/6848 , B01J19/0046 , B01J2219/00585 , B01J2219/00587 , B01J2219/00608 , B01J2219/00621 , B01J2219/00637 , B01J2219/00653 , B01J2219/00675 , B01J2219/00722 , C12Q1/6844 , C12Q1/6874 , C12Q2521/507 , C12Q2527/101 , C12Q2527/113 , C12Q2535/122 , C12Q2565/513 , C12Q2565/537
Abstract: A method including (a) providing an amplification reagent including an array of sites, and a solution having different target nucleic acids; and (b) reacting the amplification reagent to produce amplification sites each having a clonal population of amplicons from a target nucleic acid from the solution. The reacting can include simultaneously transporting the nucleic acids to the sites at an average transport rate, and amplifying the nucleic acids that transport to the sites at an average amplification rate, wherein the average amplification rate exceeds the average transport rate. The reacting can include producing a first amplicon from a nucleic acid that transports to each of the sites, and producing subsequent amplicons from the nucleic acid or from the first amplicon, wherein the average rate at which the subsequent amplicons are generated exceeds the average rate at which the first amplicon is generated.
-
公开(公告)号：US09677132B2
公开(公告)日：2017-06-13
申请号：US14575863
申请日：2014-12-18
Applicant: Illumina Cambridge Limited , Illumina, Inc.
Inventor： Roberto Rigatti , Niall Anthony Gormley , Allen E. Eckhardt , Jonathan Mark Boutell
IPC: C12Q1/68
CPC classification number: C12Q1/6874 , C12Q1/6806 , C12Q1/6837 , C12Q1/6853 , C12Q1/6869 , C12Q2521/507 , C12Q2535/122 , C12Q2565/543
Abstract: The present disclosure relates to the field of molecular biology and more specifically to methods for capturing and amplifying target polynucleotides on a solid surface.
-
公开(公告)号：US20160053310A1
公开(公告)日：2016-02-25
申请号：US14879932
申请日：2015-10-09
Applicant: ILLUMINA, INC.
Inventor： Min-Jui Richard Shen , Jonathan Mark Boutell , Kathryn M. Stephens , Mostafa Ronaghi , Kevin Gunderson , Bala Murali Venkatesan , M. Shane Bowen , Kandaswamy Vijayan
IPC: C12Q1/68
CPC classification number: C12Q1/6848 , B01J19/0046 , B01J2219/00585 , B01J2219/00587 , B01J2219/00608 , B01J2219/00621 , B01J2219/00637 , B01J2219/00653 , B01J2219/00675 , B01J2219/00722 , C12Q1/6844 , C12Q1/6874 , C12Q2521/507 , C12Q2527/101 , C12Q2527/113 , C12Q2535/122 , C12Q2565/513 , C12Q2565/537
Abstract: A method including (a) providing an amplification reagent including an array of sites, and a solution having different target nucleic acids; and (b) reacting the amplification reagent to produce amplification sites each having a clonal population of amplicons from a target nucleic acid from the solution. The reacting can include simultaneously transporting the nucleic acids to the sites at an average transport rate, and amplifying the nucleic acids that transport to the sites at an average amplification rate, wherein the average amplification rate exceeds the average transport rate. The reacting can include producing a first amplicon from a nucleic acid that transports to each of the sites, and producing subsequent amplicons from the nucleic acid or from the first amplicon, wherein the average rate at which the subsequent amplicons are generated exceeds the average rate at which the first amplicon is generated.
-
公开(公告)号：US09169513B2
公开(公告)日：2015-10-27
申请号：US14512693
申请日：2014-10-13
Applicant: ILLUMINA, INC.
Inventor： Min-Jui Richard Shen , Jonathan Mark Boutell , Kathryn M. Stephens , Mostafa Ronaghi , Kevin Gunderson , Bala Murali Venkatesan , M. Shane Bowen , Kandaswamy Vijayan
CPC classification number: C12Q1/6848 , B01J19/0046 , B01J2219/00585 , B01J2219/00587 , B01J2219/00608 , B01J2219/00621 , B01J2219/00637 , B01J2219/00653 , B01J2219/00675 , B01J2219/00722 , C12Q1/6844 , C12Q1/6874 , C12Q2521/507 , C12Q2527/101 , C12Q2527/113 , C12Q2535/122 , C12Q2565/513 , C12Q2565/537
Abstract: A method including (a) providing an amplification reagent including an array of sites, and a solution having different target nucleic acids; and (b) reacting the amplification reagent to produce amplification sites each having a clonal population of amplicons from a target nucleic acid from the solution. The reacting can include simultaneously transporting the nucleic acids to the sites at an average transport rate, and amplifying the nucleic acids that transport to the sites at an average amplification rate, wherein the average amplification rate exceeds the average transport rate. The reacting can include producing a first amplicon from a nucleic acid that transports to each of the sites, and producing subsequent amplicons from the nucleic acid or from the first amplicon, wherein the average rate at which the subsequent amplicons are generated exceeds the average rate at which the first amplicon is generated.
Abstract translation: 一种方法，包括（a）提供包含位点阵列的扩增试剂和具有不同靶核酸的溶液; 和（b）使扩增试剂反应以产生每个具有来自溶液的靶核酸的扩增子的克隆群的扩增位点。 反应可以包括以平均传输速率同时将核酸输送到位点，以及以平均扩增速率扩增转运到位点的核酸，其中平均扩增速率超过平均传输速率。 该反应可以包括从转运到每个位点的核酸产生第一扩增子，并产生来自核酸或第一扩增子的后续扩增子，其中后续扩增子产生的平均速率超过在 其中生成第一个扩增子。
-
公开(公告)号：US20130338042A1
公开(公告)日：2013-12-19
申请号：US13783043
申请日：2013-03-01
Applicant: ILLUMINA, INC.
Inventor： Min-Jui Richard Shen , Jonathan Mark Boutell , Kathryn M. Stephens , Mostafa Ronaghi , Kevin Gunderson , Bala Murali Venkatesan , M. Shane Bowen , Kandaswamy Vijayan
IPC: B01J19/00
CPC classification number: C12Q1/6848 , B01J19/0046 , B01J2219/00585 , B01J2219/00587 , B01J2219/00608 , B01J2219/00621 , B01J2219/00637 , B01J2219/00653 , B01J2219/00675 , B01J2219/00722 , C12Q1/6844 , C12Q1/6874 , C12Q2521/507 , C12Q2527/101 , C12Q2527/113 , C12Q2535/122 , C12Q2565/513 , C12Q2565/537
Abstract: A method including (a) providing an amplification reagent including an array of sites, and a solution having different target nucleic acids; and (b) reacting the amplification reagent to produce amplification sites each having a clonal population of amplicons from a target nucleic acid from the solution. The reacting can include simultaneously transporting the nucleic acids to the sites at an average transport rate, and amplifying the nucleic acids that transport to the sites at an average amplification rate, wherein the average amplification rate exceeds the average transport rate. The reacting can include producing a first amplicon from a nucleic acid that transports to each of the sites, and producing subsequent amplicons from the nucleic acid or from the first amplicon, wherein the average rate at which the subsequent amplicons are generated exceeds the average rate at which the first amplicon is generated.
-
-
-
-
-
-
-
-
-
Search Results
40
records
(took 0.042 seconds)
