-
1.发明公开公开(公告)号：US20230407388A1
公开(公告)日：2023-12-21
申请号：US18303905
申请日：2023-04-20
IPC分类号： C12Q1/6874
CPC分类号： C12Q1/6874
摘要： Described herein is a polynucleotide for use as a sequencing template comprising multiple inserts. Also described herein are method of generating and using these polynucleotides and methods of use of such templates, including analysis of contiguity information. Further, sequencing templates comprising an insert sequence and a copy of the insert sequence can be used to correct for random errors generated during sequencing or amplification or to identify nucleobase damage or other mutation that leads to non-canonical base pairing in a double-stranded nucleic acid. Methods of performing methylation analysis are also described herein.
-
2.发明公开公开(公告)号：US20240117416A1
公开(公告)日：2024-04-11
申请号：US18274974
申请日：2022-01-28
IPC分类号： C12Q1/6834 , C12Q1/6844 , C12Q1/6874
CPC分类号： C12Q1/6834 , C12Q1/6844 , C12Q1/6874
摘要： The disclosure relates to methods, compositions, and kits for improving seeding efficiency of flow cells with polynucleotides, and applications thereof, including for sequencing.
-
公开(公告)号：US11938475B2
公开(公告)日：2024-03-26
申请号：US17114134
申请日：2020-12-07
发明人： Hongji Ren , Jonathan Mark Boutell , John A. Moon , M. Shane Bowen , Alex Nemiroski , Gary Mark Skinner , Kenny Chen
IPC分类号： B01L3/00 , B01J19/00 , C08F220/56 , C08F220/60 , C09D133/26 , C12Q1/6876
CPC分类号： B01L3/502 , B01J19/0046 , C08F220/56 , C08F220/603 , C09D133/26 , C12Q1/6876 , B01J2219/00475 , B01J2219/00729 , B01L2200/12 , B01L2300/0893 , B01L2300/16
摘要： In an example of the method, a functionalized coating layer is applied in depressions of a patterned flow cell substrate. The depressions are separated by interstitial regions. A primer is grafted to the functionalized coating layer to form a grafted functionalized coating layer in the depressions. A hydrogel is applied on at least the grafted functionalized coating layer.
-
公开(公告)号：US10919033B2
公开(公告)日：2021-02-16
申请号：US16626393
申请日：2018-12-17
发明人： Hongji Ren , Jonathan Mark Boutell , John A. Moon , M. Shane Bowen , Alex Nemiroski , Gary Mark Skinner , Kenny Chen
IPC分类号： C12Q1/6876 , B01L3/00 , C08F220/60 , B01J19/00 , C08F220/56 , C09D133/26
摘要： In an example of the method, a functionalized coating layer is applied in depressions of a patterned flow cell substrate. The depressions are separated by interstitial regions. A primer is grafted to the functionalized coating layer to form a grafted functionalized coating layer in the depressions. A hydrogel is applied on at least the grafted functionalized coating layer.
-
公开(公告)号：US20230212667A1
公开(公告)日：2023-07-06
申请号：US18089867
申请日：2022-12-28
发明人： Jonathan Mark Boutell , Eli M. Carrami , Pietro Gatti Lafranconi , Philip Balding , Oliver Jon Miller , Kay Klausing , Justin Robbins , Xiaolin Wu
IPC分类号： C12Q1/6874
CPC分类号： C12Q1/6874 , C12Q1/6806
摘要： Polynucleotide sequencing methods for sequencing one or more polynucleotide templates use primers bound to a surface as sequencing primers. The surface primers may include at least a portion of a surface oligonucleotide used during cluster formation. The sequencing methods may be used for single stranded sequencing or double stranded sequencing. Double stranded sequencing methods may employ an enzyme that has nick-translation activity. A kit includes all the reagents needed for sequencing does not include sequencing primers. The kit may be used to accomplish the sequencing methods of the present disclosure.
-
公开(公告)号：US20210086174A1
公开(公告)日：2021-03-25
申请号：US17114134
申请日：2020-12-07
发明人： Hongji Ren , Jonathan Mark Boutell , John A. Moon , M. Shane Bowen , Alex Nemiroski , Gary Mark Skinner , Kenny Chen
IPC分类号： B01L3/00 , C08F220/60 , B01J19/00 , C08F220/56 , C09D133/26 , C12Q1/6876
摘要： In an example of the method, a functionalized coating layer is applied in depressions of a patterned flow cell substrate. The depressions are separated by interstitial regions. A primer is grafted to the functionalized coating layer to form a grafted functionalized coating layer in the depressions. A hydrogel is applied on at least the grafted functionalized coating layer.
-
公开(公告)号：US09944924B2
公开(公告)日：2018-04-17
申请号：US14796753
申请日：2015-07-10
CPC分类号： C12N15/1068 , C12Q1/6806 , C12Q1/6837 , C12Q1/6853 , C12Q1/6874 , C40B50/06 , C12Q2525/204 , C12Q2565/543
摘要： The present disclosure relates to the field of molecular biology and more specifically to methods for capturing and amplifying target polynucleotides on a solid surface.
-
8.发明申请公开(公告)号：US20210230689A1
公开(公告)日：2021-07-29
申请号：US17227639
申请日：2021-04-12
发明人： Eric Vermaas , Mahdieh Khosroheidari , Angela Kalbande , Vincent Peter Smith , Claire Bevis-Mott , Jonathan Mark Boutell , Michael Chesney
IPC分类号： C12Q1/6874 , C12Q1/6809 , C12Q1/6816 , C12Q1/6834 , C12Q1/6855 , C12Q1/6806
摘要： The present invention is concerned with compositions and methods for improving the rate of correct sample identification in indexed nucleic acid library preparations for multiplex next generation sequencing by blocking the 3′ ends of pooled indexed polynucleotides from multiple samples prior to amplification and sequencing, by exonuclease treatment and optionally blocking the 3′ ends of pooled indexed polynucleotides from multiple samples prior to amplification and sequencing, by exonuclease treatment after protective adapters are ligated to target polynucleotides to degrade unincorporated adapters prior to amplification and sequencing, and/or by modifying or blocking 5′ and 3′ ends of pooled indexed polynucleotides from multiple samples, with an optional exonuclease treatment, prior to amplification and sequencing.
-
公开(公告)号：US10901230B2
公开(公告)日：2021-01-26
申请号：US16311652
申请日：2017-06-20
发明人： Gary Mark Skinner , Geraint Wyn Evans , Stanley S. Hong , John A. Moon , M. Shane Bowen , Jonathan Mark Boutell , Jason Richard Betley
摘要： Example super-resolution microscopy systems are described herein that are configured for relatively high throughput. The disclosed microscopy systems can be to generate an array of sub-diffraction activated areas for imaging. The microscopy systems can be to utilize imaging techniques that employ time delay integration to build up super-resolution images over time. The disclosed microscopy systems can utilize long-lived fluorophores in conjunction with wide field and patterned illumination to generate super-resolution images of a sample with relatively high throughput.
-
公开(公告)号：US20230279469A1
公开(公告)日：2023-09-07
申请号：US18146320
申请日：2022-12-23
发明人： Johan Sebastian Basuki , Jonathan Mark Boutell , Jeffrey S. Fisher , Louise Jane Fraser , Wayne N. George , Niall Anthony Gormley , David Jones , Xiaoyu Ma , Maria Ines Martins Vitoriano , Zhong Mei , Oliver Jon Miller , Andrew Price , Sebastien Georg Gabriel Ricoult , Vicki S. Thomson , Jacqueline C. Weir , Xiaoy Ma , Weihua Chang , Hui Han
IPC分类号： C12Q1/6806 , C12N15/10 , B01L3/00
CPC分类号： C12Q1/6806 , B01L3/502761 , C12N15/1065 , B01L2200/0647 , B01L2300/0893
摘要： An example of a flow cell includes a substrate having depressions separated by interstitial regions. First and second primers are immobilized within the depressions. First transposome complexes are immobilized within the depressions, and the first transposome complexes include a first amplification domain. Second transposome complexes are also immobilized within the depressions, and the second transposome complexes include a second amplification domain. Some of the first transposome complexes, or some of the second transposome complexes, or some of both of the first and second transposome complexes include a modification to reduce tagmentation efficiency.
-
-
-
-
-
-
-
-
-
搜索结果
104 条记录 (耗时 0.02 秒)
