公开(公告)号：US20070110633A1

公开(公告)日：2007-05-17

申请号：US11617809

申请日：2006-12-29

申请人： Steven Sundberg ,  J. Parce ,  Calvin Chow

发明人： Steven Sundberg ,  J. Parce ,  Calvin Chow

IPC分类号： B01L3/00

CPC分类号： G01N27/44791 ,  B01J19/0093 ,  B01J2219/00783 ,  B01J2219/00826 ,  B01J2219/00831 ,  B01J2219/00833 ,  B01J2219/00853 ,  B01J2219/00891 ,  B01J2219/00909 ,  B01L3/0244 ,  B01L3/0262 ,  B01L3/5025 ,  B01L3/50255 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/502723 ,  B01L3/50273 ,  B01L3/502753 ,  B01L2200/027 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0864 ,  B01L2400/0406 ,  B01L2400/0418 ,  B01L2400/0421 ,  B01L2400/0487 ,  B01L2400/0688 ,  G01N27/44743 ,  G01N2035/00237 ,  G01N2035/1037 ,  Y10T436/25125 ,  Y10T436/25375 ,  Y10T436/2575

摘要： Fluid introduction is facilitated through the use of a port which extends entirely through a microfluidic substrate. Capillary forces can be used to retain the fluid within the port, and a series of samples or other fluids may be introduced through a single port by sequentially blowing the fluid out through the substrate and replacing the removed fluid with an alternate fluid, or by displacing the fluid in part with additional fluid. In another aspect, microfluidic substrates have channels which varying in cross-sectional dimension so that capillary action spreads a fluid only within a limited portion of the channel network. In yet another aspect, the introduction ports may include a multiplicity of very small channels leading from the port to a fluid channel, so as to filter out particles or other contaminants which might otherwise block the channel at the junction between the channel and the introduction port.

公开(公告)号：US20060286002A1

公开(公告)日：2006-12-21

申请号：US11493998

申请日：2006-07-27

申请人： Steven Sundberg ,  J. Parce ,  Calvin Chow

发明人： Steven Sundberg ,  J. Parce ,  Calvin Chow

IPC分类号： B01L3/02

CPC分类号： G01N27/44791 ,  B01J19/0093 ,  B01J2219/00783 ,  B01J2219/00826 ,  B01J2219/00831 ,  B01J2219/00833 ,  B01J2219/00853 ,  B01J2219/00891 ,  B01J2219/00909 ,  B01L3/0244 ,  B01L3/0262 ,  B01L3/5025 ,  B01L3/50255 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/502723 ,  B01L3/50273 ,  B01L3/502753 ,  B01L2200/027 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0864 ,  B01L2400/0406 ,  B01L2400/0418 ,  B01L2400/0421 ,  B01L2400/0487 ,  B01L2400/0688 ,  G01N27/44743 ,  G01N2035/00237 ,  G01N2035/1037 ,  Y10T436/25125 ,  Y10T436/25375 ,  Y10T436/2575

摘要： Fluid introduction is facilitated through the use of a port which extends entirely through a microfluidic substrate. Capillary forces can be used to retain the fluid within the port, and a series of samples or other fluids may be introduced through a single port by sequentially blowing the fluid out through the substrate and replacing the removed fluid with an alternate fluid, or by displacing the fluid in part with additional fluid. In another aspect, microfluidic substrates have channels which varying in cross-sectional dimension so that capillary action spreads a fluid only within a limited portion of the channel network. In yet another aspect, the introduction ports may include a multiplicity of very small channels leading from the port to a fluid channel, so as to filter out particles or other contaminants which might otherwise block the channel at the junction between the channel and the introduction port.

公开(公告)号：US20060286001A1

公开(公告)日：2006-12-21

申请号：US11493997

申请日：2006-07-27

申请人： Steven Sundberg ,  J. Parce ,  Calvin Chow

发明人： Steven Sundberg ,  J. Parce ,  Calvin Chow

IPC分类号： B01L3/02

CPC分类号： G01N27/44791 ,  B01J19/0093 ,  B01J2219/00783 ,  B01J2219/00826 ,  B01J2219/00831 ,  B01J2219/00833 ,  B01J2219/00853 ,  B01J2219/00891 ,  B01J2219/00909 ,  B01L3/0244 ,  B01L3/0262 ,  B01L3/5025 ,  B01L3/50255 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/502723 ,  B01L3/50273 ,  B01L3/502753 ,  B01L2200/027 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0864 ,  B01L2400/0406 ,  B01L2400/0418 ,  B01L2400/0421 ,  B01L2400/0487 ,  B01L2400/0688 ,  G01N27/44743 ,  G01N2035/00237 ,  G01N2035/1037 ,  Y10T436/25125 ,  Y10T436/25375 ,  Y10T436/2575

摘要： Fluid introduction is facilitated through the use of a port which extends entirely through a microfluidic substrate. Capillary forces can be used to retain the fluid within the port, and a series of samples or other fluids may be introduced through a single port by sequentially blowing the fluid out through the substrate and replacing the removed fluid with an alternate fluid, or by displacing the fluid in part with additional fluid. In another aspect, microfluidic substrates have channels which varying in cross-sectional dimension so that capillary action spreads a fluid only within a limited portion of the channel network. In yet another aspect, the introduction ports may include a multiplicity of very small channels leading from the port to a fluid channel, so as to filter out particles or other contaminants which might otherwise block the channel at the junction between the channel and the introduction port.

公开(公告)号：US20060275817A1

公开(公告)日：2006-12-07

申请号：US11460550

申请日：2006-08-11

申请人： J. Parce ,  Theo Nikiforov ,  Tammy Mehta ,  Anne Kopf-Sill ,  Andrea Chow ,  Michael Knapp

发明人： J. Parce ,  Theo Nikiforov ,  Tammy Mehta ,  Anne Kopf-Sill ,  Andrea Chow ,  Michael Knapp

IPC分类号： C12Q1/68 ,  C07H21/04

CPC分类号： C12Q1/6874 ,  B01F13/0059 ,  B01J2219/00317 ,  B01J2219/00459 ,  B01J2219/00468 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/502761 ,  B01L2200/0668 ,  B01L2400/0409 ,  B01L2400/0415 ,  B01L2400/043 ,  B01L2400/0487 ,  C07H21/02 ,  C07H21/04 ,  C12Q1/6869 ,  C40B60/14 ,  G01N27/44756 ,  G01N35/0098 ,  G01N2035/00574 ,  Y10T436/143333 ,  C12Q2565/629 ,  C12Q2535/101 ,  C12Q2563/173 ,  C12Q2525/186 ,  C12Q2527/125

摘要： Nucleotides and nucleotide analogs are used in various sequencing by incorporation/sequencing by synthesis methods. Nucleotide analogs comprising 3′-blocking groups are used to provide reversible chain-termination for sequencing by synthesis. Typical blocking groups include phosphate groups and carbamate groups. Fluorescent nucleotides are used to perform sequencing by synthesis with detection by incorporation of the fluorescently labeled nucleotide, optionally followed by photobleaching and intercalating dyes are used to detect addition of a non-labeled nucleotide in sequencing by synthesis with detection by intercalation. Microfluidic devices, including particle arrays, are used in the sequencing methods.

摘要翻译： 核苷酸和核苷酸类似物通过合成方法的并入/测序用于各种测序。 包含3'-封闭基团的核苷酸类似物用于提供可逆的链终止，用于通过合成进行测序。 典型的封闭基团包括磷酸基和氨基甲酸酯基。 使用荧光核苷酸进行测序，通过掺入荧光标记的核苷酸进行检测，任选地随后进行光漂白，并使用嵌入染料检测通过插入检测合成测序中的未标记核苷酸的添加。 微流控器件，包括粒子阵列，用于测序方法。

公开(公告)号：US20050221385A1

公开(公告)日：2005-10-06

申请号：US11051445

申请日：2005-02-03

申请人： Theo Nikiforov ,  Jill Baker ,  Sansan Lin ,  J. Parce ,  Jude Dunne ,  Javier Farinas ,  Esther Huang ,  Mingqing Li ,  Thi Trinh

发明人： Theo Nikiforov ,  Jill Baker ,  Sansan Lin ,  J. Parce ,  Jude Dunne ,  Javier Farinas ,  Esther Huang ,  Mingqing Li ,  Thi Trinh

IPC分类号： G01N30/88 ,  G01N33/53

CPC分类号： G01N30/88

摘要： Methods for chromatographically separating materials, including the separation of materials in kinase or phosphatase assays, in microfluidic devices under positive or negative fluid pressure. Devices and integrated systems for performing chromatographic separations are also provided.

摘要翻译： 用于在正或负流体压力下在微流体装置中色谱分离材料的方法，包括在激酶或磷酸酶测定中分离材料。 还提供了用于执行色谱分离的设备和集成系统。

公开(公告)号：US20050148084A1

公开(公告)日：2005-07-07

申请号：US11069632

申请日：2005-02-28

申请人： J. Parce ,  Morten Jensen

发明人： J. Parce ,  Morten Jensen

IPC分类号： B01L3/00 ,  G01N35/00

CPC分类号： B01L3/5027 ,  B01L2200/143 ,  G01N35/0092 ,  G01N2035/0093 ,  Y10T436/115831 ,  Y10T436/25375 ,  Y10T436/2575

摘要： Techniques for controlling analytical instruments are provided. A sequence of steps can be utilized to specify wells of a microfluidic device, mobility to be applied to fluid in the wells, and the duration to apply the mobility. For example, fluids can be sequentially run past down a main channel to a detection zone of the microfluidic device in order to analyze the fluids. In order to increase the efficiency of the analysis, fluids can be processed in parallel by running one fluid down the main channel while another fluid is loaded to the main channel.

摘要翻译： 提供了控制分析仪器的技术。 可以使用一系列步骤来指定微流体装置的孔，将要施加到井中的流体的移动性以及施加移动性的持续时间。 例如，为了分析流体，流体可以顺序地沿主通道向下流动到微流体装置的检测区域。 为了提高分析效率，可以通过在主通道下方运行一个流体同时将另一个流体加载到主通道来并行处理流体。

公开(公告)号：US20060258019A1

公开(公告)日：2006-11-16

申请号：US11461163

申请日：2006-07-31

申请人： Andrea Chow ,  Anne Kopf-Sill ,  J. Parce ,  Robert Dubrow

发明人： Andrea Chow ,  Anne Kopf-Sill ,  J. Parce ,  Robert Dubrow

IPC分类号： B01L3/00

CPC分类号： B01D61/18 ,  B01L3/5025 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/502723 ,  B01L3/50273 ,  B01L2200/027 ,  B01L2300/0861 ,  B01L2300/0867 ,  B01L2400/0406 ,  B01L2400/0415 ,  B01L2400/043 ,  B01L2400/0457 ,  Y10T137/2496 ,  Y10T137/2499 ,  Y10T137/2501 ,  Y10T137/2514 ,  Y10T137/2516 ,  Y10T436/11 ,  Y10T436/2575

摘要： Methods and devices for delivering fluids into microfluidic device body structures are described. The methods and devices include the use of fluid manifolds that are integrated or interchangeable with device body structures. Methods of fabricating manifolds are also provided.

摘要翻译： 描述了将流体输送到微流体装置体结构中的方法和装置。 所述方法和装置包括使用与装置主体结构集成或可互换的流体歧管。 还提供制造歧管的方法。

公开(公告)号：US20050279635A1

公开(公告)日：2005-12-22

申请号：US11005120

申请日：2004-12-06

申请人： Calvin Chow ,  J. Parce ,  Richard McReynolds ,  Colin Kennedy ,  Luc Bousse

发明人： Calvin Chow ,  J. Parce ,  Richard McReynolds ,  Colin Kennedy ,  Luc Bousse

IPC分类号： B01J19/00 ,  B01L3/00 ,  B01L9/00 ,  C25B11/00 ,  G01N27/27 ,  G01N27/447 ,  G01N27/453

CPC分类号： G01N27/453 ,  B01J19/0093 ,  B01J2219/00853 ,  B01L3/502715 ,  B01L9/527 ,  B01L2200/027 ,  B01L2300/0816 ,  B01L2400/0415 ,  G01N27/44743 ,  G01N27/44791 ,  Y10T436/2575

摘要： The present invention is generally directed to improved methods, structures and systems for interfacing microfluidic devices with ancillary systems that are used in conjunction with such devices. These systems typically include control and monitoring systems (620) for controlling the performance of the processes carried out within the device, e.g., monitoring and controlling environmental conditions and monitoring results of the processes performed, e.g., detection.

摘要翻译： 本发明总体上涉及用于将微流体装置与与这种装置结合使用的辅助系统进行接口的改进的方法，结构和系统。 这些系统通常包括用于控制在设备内执行的过程的性能的控制和监视系统（620），例如监视和控制环境条件并监视所执行的过程的结果，例如检测。

公开(公告)号：US20070177147A1

公开(公告)日：2007-08-02

申请号：US11728629

申请日：2007-03-26

申请人： J. Parce

发明人： J. Parce

IPC分类号： G01N27/26

CPC分类号： G01N27/44791 ,  G01N27/44721 ,  Y10S366/02

摘要： The present invention provides a microfluidic system for electrophoretic analysis of materials in the fields of chemistry, biochemistry, biotechnology, molecular biology and numerous other fields. Light absorbance signals are received by a photodetector from periodically spaced regions along a channel in the microfluidic system. The signals received by the photodetector are modulated by the movement of species bands through the channel under electrophoretic forces. By Fourier analysis, the velocity of each species band is determined, and identification of the species is made based on its electrophoretic mobility in the channel.

摘要翻译： 本发明提供了用于化学，生物化学，生物技术，分子生物学等许多领域的材料的电泳分析的微流体系统。 光吸收信号由光电检测器沿着微流体系统中的通道的周期性间隔的区域接收。 由光电探测器接收的信号通过电泳力通过通道的物种带的移动来调制。 通过傅立叶分析，确定每个物种带的速度，并根据其在通道中的电泳迁移率来确定物种的鉴定。

公开(公告)号：US20070157973A1

公开(公告)日：2007-07-12

申请号：US11702368

申请日：2007-02-03

申请人： Ring-Ling Chien ,  J. Parce ,  Andrea Chow ,  Anne Kopf-Sill

发明人： Ring-Ling Chien ,  J. Parce ,  Andrea Chow ,  Anne Kopf-Sill

IPC分类号： F15C1/04 ,  F04B41/06

CPC分类号： B01L9/527 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502738 ,  B01L3/502746 ,  B01L2200/025 ,  B01L2200/027 ,  B01L2200/146 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2400/0415 ,  B01L2400/0487 ,  B01L2400/082 ,  G01N11/08 ,  G01N27/44791 ,  G01N2035/1044 ,  G05D7/0694 ,  Y10T137/2076 ,  Y10T137/218 ,  Y10T137/86131

摘要： Improved microfluidic devices, systems, and methods allow selective transportation of fluids within microfluidic channels of a microfluidic network by applying, controlling, and varying pressures at a plurality of reservoirs. Modeling the microfluidic network as a series of nodes connected together by channel segments and determining the flow resistance characteristics of the channel segments may allow calculation of fluid flows through the channel segments resulting from a given pressure configuration at the reservoirs. To effect a desired flow within a particular channel or series of channels, reservoir pressures may be identified using the network model. Viscometers or other flow sensors may measure flow characteristics within the channels, and the measured flow characteristics can be used to calculate pressures to generate a desired flow. Multi-reservoir pressure modulator and pressure controller systems can optionally be used in conjunction with electrokinetic or other fluid transport mechanisms.