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50 条记录 (耗时 0.051 秒)

    Method for separating an analyte from a sample
    33.
    发明授权
    Method for separating an analyte from a sample 有权
    从样品中分离分析物的方法

    公开(公告)号：US08247176B2

    公开(公告)日：2012-08-21

    申请号：US13042203

    申请日：2011-03-07

    申请人： Kurt E. Petersen ,  William A. McMillan ,  Lee A. Christel ,  Ronald Chang ,  Farzad Pourahmadi ,  Jesus Ching ,  Gregory T. A. Kovacs ,  M. Allen Northrup

    发明人： Kurt E. Petersen ,  William A. McMillan ,  Lee A. Christel ,  Ronald Chang ,  Farzad Pourahmadi ,  Jesus Ching ,  Gregory T. A. Kovacs ,  M. Allen Northrup

    IPC分类号： C12Q1/68

    CPC分类号： C12N15/1017 ,  B01D15/00 ,  B01L7/52 ,  B01L2400/0622 ,  B01L2400/0644 ,  C12M47/06 ,  G01N1/34 ,  Y10T436/255

    摘要： An analyte is separated from a fluid sample by introducing the sample into a cartridge having a sample port and a first flow path extending from the sample port. The first flow path includes an extraction chamber containing a solid support for capturing the analyte from the sample. The cartridge has a second flow path for eluting the captured analyte from the extraction chamber, the second flow diverging from the first flow path after passing through the extraction chamber. The sample is forced to flow through the extraction chamber and into a waste chamber, thereby capturing the analyte with the solid support as the sample flows through the extraction chamber. The captured analyte is then eluted from the extraction chamber by forcing an elution fluid to flow through the extraction chamber and along the second flow path.

    摘要翻译： 将样品通过将样品引入具有样品端口和从样品端口延伸的第一流路的盒中而将分析物与流体样品分离。 第一流路包括提取室，其包含用于从样品捕获分析物的固体支持物。 所述筒具有用于从所述提取室洗脱所捕获的分析物的第二流动路径，所述第二流在通过所述提取室之后从所述第一流动路径发散。 样品被迫流过提取室并进入废物室，从而当样品流过提取室时用固体支持物捕获分析物。 然后通过迫使洗脱液体流过提取室并沿着第二流动路径，将捕获的分析物从萃取室洗脱。

    Cartridge for analyzing a fluid sample
    39.
    发明授权
    Cartridge for analyzing a fluid sample 有权
    用于分析流体样品的墨盒

    公开(公告)号：US06783736B1

    公开(公告)日：2004-08-31

    申请号：US09801077

    申请日：2001-03-06

    申请人： Michael T. Taylor ,  Farzad Pourahmadi ,  Ronald Chang ,  Douglas B. Dority

    发明人： Michael T. Taylor ,  Farzad Pourahmadi ,  Ronald Chang ,  Douglas B. Dority

    IPC分类号： B01L1100

    CPC分类号： B01L3/502 ,  B01L7/52 ,  B01L2200/10 ,  B01L2300/0654 ,  B01L2300/0681 ,  B01L2300/0809 ,  B01L2300/087 ,  B01L2400/0487 ,  B01L2400/0622 ,  B01L2400/0644 ,  C12N1/066 ,  C12N13/00 ,  Y10S435/81 ,  Y10S436/805 ,  Y10S436/806 ,  Y10S436/807 ,  Y10S436/809 ,  Y10S436/829 ,  Y10T137/87716 ,  Y10T137/88046 ,  Y10T436/255

    摘要： A cartridge for analyzing a fluid sample has a body defining at least first and second channels and a cavity separating the channels. An end of the first channel is positioned on a first side of the cavity, and an end of the second channel is positioned on a second side of the cavity. The cavity is defined by a first curved surface positioned adjacent the end of the first channel, a second curved surface positioned adjacent the end of the second channel, and at least a third surface between the first and second curved surfaces. The cartridge also comprises an elastic membrane for establishing a seal with the first and second curved surfaces to prevent the flow of fluid between the channels. The third surface is recessed from the first and second surfaces to provide a gap between the membrane and the third surface when the membrane is pressed against the first and second surfaces. A cartridge having a conical valve seat is also disclosed.

    摘要翻译： 用于分析流体样品的盒具有至少限定第一和第二通道的主体和分隔通道的空腔。 第一通道的端部位于空腔的第一侧上，并且第二通道的端部位于空腔的第二侧。 空腔由邻近第一通道的端部定位的第一弯曲表面，邻近第二通道的端部定位的第二弯曲表面以及第一和第二弯曲表面之间的至少第三表面限定。 墨盒还包括用于与第一和第二曲面建立密封以防止流体在通道之间流动的弹性膜。 第三表面从第一表面和第二表面凹进，当膜压在第一表面和第二表面上时，在膜和第三表面之间提供间隙。 还公开了一种具有锥形阀座的盒。

    Apparatus and method for rapid ultrasonic disruption of cells or viruses
    40.
    发明授权
    Apparatus and method for rapid ultrasonic disruption of cells or viruses 有权
    用于细胞或病毒快速超声破碎的装置和方法

    公开(公告)号：US06431476B1

    公开(公告)日：2002-08-13

    申请号：US09469724

    申请日：1999-12-21

    申请人： Michael T. Taylor ,  Phillip Belgrader ,  Farzad Pourahmadi ,  William A. McMillan ,  Ronald Chang ,  Douglas B. Dority

    发明人： Michael T. Taylor ,  Phillip Belgrader ,  Farzad Pourahmadi ,  William A. McMillan ,  Ronald Chang ,  Douglas B. Dority

    IPC分类号： B02C1900

    CPC分类号： B01L3/502 ,  B01L3/50825 ,  B01L7/52 ,  B01L2300/042 ,  B01L2300/046 ,  B01L2300/14 ,  B01L2400/0439 ,  B01L2400/0622 ,  B01L2400/0644 ,  C12M47/06

    摘要： The present invention provides an apparatus and method for disrupting cells or viruses to release the nucleic acid therefrom. The apparatus includes a container having a chamber for holding the cells or viruses. The apparatus also includes an ultrasonic transducer for contacting a wall of the chamber and for transmitting ultrasonic energy into the chamber through the wall. A support structure holds the container and the transducer against each other such that the transducer contacts the wall of the chamber. The support structure includes an elastic body, such as a spring, for applying to the container or to the transducer a substantially constant force to press together the transducer and the wall. The chamber also preferably contains beads for enhancing the disruption of the cells or viruses. The apparatus performs rapid and consistent lysis of cells or viruses, often in as little time as 5 to 10 seconds.

    摘要翻译： 本发明提供了用于破坏细胞或病毒从其释放核酸的装置和方法。 该装置包括具有用于保持细胞或病毒的室的容器。 该装置还包括用于接触室的壁并用于通过壁将超声波能量传递到室中的超声换能器。 支撑结构将容器和换能器彼此抵靠，使得换能器接触室的壁。 支撑结构包括诸如弹簧的弹性体，用于向容器或换能器施加基本恒定的力以将换能器和壁压在一起。 该室还优选含有用于增强细胞或病毒破坏的珠粒。 该装置通常在5至10秒的短时间内快速且一致地裂解细胞或病毒。