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63 条记录 (耗时 0.047 秒)

    Methods for amplification of nucleic acids on solid support
    32.
    发明授权
    Methods for amplification of nucleic acids on solid support 有权

    公开(公告)号：US10174352B2

    公开(公告)日：2019-01-08

    申请号：US14773362

    申请日：2014-03-14

    申请人： Lyle J. Arnold ,  Norman C. Nelson

    发明人： Lyle J. Arnold ,  Norman C. Nelson

    IPC分类号： C12Q1/68 ,  C12P19/34 ,  C12Q1/6853 ,  C12Q1/6865 ,  C07H21/02

    摘要： The present invention provides methods for amplifying a nucleic acid from a sample containing a mixture of nucleic acids utilizing a solid support. Methods are provided utilizing user-defined primer oligonucleotides for directional amplification that assists in further manipulation of the target nucleic acid, such as sequencing. Methods are also provided utilizing blocker and displacer oligonucleotides for generating amplified target nucleic acids of defined length. One of these methods provides a first oligonucleotide and a second oligonucleotide affixed to a solid support or separate solid supports. The first oligonucleotide is blocked to prevent extension from the 3′-terminus and has a sequence complementary to a first portion of a target nucleic acid. The second oligonucleotide has a sequence that is identical to a second portion of the target nucleic acid. In this method, a sample is applied to the solid support and the target nucleic acid within the sample binds said first oligonucleotide. The solid support is then washed to remove unbound nucleic acids. A primer sequence containing a target binding region and a polymerase promoter sequence is then annealed to the bound target nucleic acid and extended producing a first duplex nucleic acid. The target sequence is then removed leaving a first nucleic acid that can now bind the second oligonucleotide. The second oligonucleotide is extended to produce a second duplex nucleic acid that contains a second nucleic acid. The second nucleic acid is then amplified by adding a polymerase.

    Extended dynamic range assays
    39.
    发明授权
    Extended dynamic range assays 失效
    扩展动态范围测定

    公开(公告)号：US06180340B2

    公开(公告)日：2001-01-30

    申请号：US08962033

    申请日：1997-10-31

    申请人： Norman C. Nelson

    发明人： Norman C. Nelson

    IPC分类号： G01N33533

    CPC分类号： G01N33/54306 ,  C12Q1/6816 ,  Y10S435/962 ,  Y10S435/968 ,  Y10S436/80 ,  Y10S436/814 ,  Y10S436/815 ,  Y10S436/817

    摘要： A method and compositions for the detection and/or quantification of an analyte through the use of a plurality of labeled probes, with two or more said probes targeted to different regions of said analyte. In specific embodiments, the labels are separately distinguishable, and/or are present at different specific activities on the labels.

    摘要翻译： 一种用于通过使用多个标记探针来检测和/或定量分析物的方法和组合物，其中两个或多个所述探针靶向所述分析物的不同区域。 在具体实施方案中，标签是分开区分的，和/或以不同的特定活性存在于标签上。