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48 条记录 (耗时 0.028 秒)

    Method for High Throughput Screening for Anitbodies and Proteins Inducing Apoptosis
    32.
    发明申请
    Method for High Throughput Screening for Anitbodies and Proteins Inducing Apoptosis 审中-公开
    高通量筛选诱导细胞凋亡的方法

    公开(公告)号：US20080145364A1

    公开(公告)日：2008-06-19

    申请号：US11816074

    申请日：2006-02-15

    申请人： Sherman M. Weissman ,  Michael Snyder

    发明人： Sherman M. Weissman ,  Michael Snyder

    IPC分类号： A61K39/395 ,  C40B30/06 ,  C07K16/18 ,  A61P43/00 ,  A61K38/00 ,  C07K1/00

    CPC分类号： G01N33/6854 ,  C07K2317/73 ,  G01N33/5011 ,  G01N2510/00

    摘要： High throughput assays used to identify antibodies and proteins that induce cell death are described herein. It is not necessary to identify the antigens the antibodies are reactive with prior to performing the assays. Instead, libraries of antibodies and proteins, including murine, human, humanized, single chain, and synthetic antibodies, are screened using high throughput assays to identify those antibodies and proteins which cause cell death. Standard technology is then used to screen for cell viability. Antibodies and proteins which induce apoptosis preferentially or exclusively of cancer cells are then isolated, characterized, and may be cloned. A method for cloning antibodies and proteins has been developed, which provides means for rapid identification of the antibody or protein and the gene encoding the antibody or protein, based on the presence of a “bar code” or “unique sequence.” A method for high throughput production of antibodies to human proteins has also been developed.

    摘要翻译： 本文描述了用于鉴定诱导细胞死亡的抗体和蛋白质的高通量测定。 在进行测定之前，不需要鉴定抗体与抗体反应的抗原。 相反，使用高通量测定法筛选抗体和蛋白质的文库，包括鼠，人，人源化，单链和合成抗体，以鉴定导致细胞死亡的那些抗体和蛋白质。 然后使用标准技术筛选细胞活力。 然后分离，表征并且可以克隆诱导凋亡优先或排除癌细胞的抗体和蛋白质。 已经开发了用于克隆抗体和蛋白质的方法，其基于“条形码”或“唯一序列”的存在，提供用于快速鉴定抗体或蛋白质和编码抗体或蛋白质的基因的方法。 还开发了用于高通量生产人类蛋白质的抗体的方法。

    Temporary Keratoprosthesis, Corneal Transplant Suturing Jig, Temporary Endoprosthesis, and Method of Using
    36.
    发明公开
    Temporary Keratoprosthesis, Corneal Transplant Suturing Jig, Temporary Endoprosthesis, and Method of Using 审中-公开

    公开(公告)号：US20240341950A1

    公开(公告)日：2024-10-17

    申请号：US18634470

    申请日：2024-04-12

    申请人： Michael Snyder

    发明人： Michael Snyder

    IPC分类号： A61F2/14 ,  A61F9/007

    CPC分类号： A61F2/142 ,  A61F2/148 ,  A61F9/007

    摘要： A temporary keratoprosthesis, a corneal transplant suturing jig, a temporary endoprosthesis, methods of using the same, and kits comprising the same. The temporary keratoprosthesis and corneal transplant comprise grooves into which sutures are located during ophthalmological surgery such that the sutures are prevented from migrating and/or obscuring a view into the eye during surgery. The temporary keratoprosthesis, corneal transplant suturing jig, and temporary endoprosthesis are employed alone or in unison to maintain intraocular pressure during surgery.