公开(公告)号：US06368804B1

公开(公告)日：2002-04-09

申请号：US09614914

申请日：2000-07-12

申请人： Arie Ben-Bassat ,  Robert Dicosimo ,  Robert Donald Fallon

发明人： Arie Ben-Bassat ,  Robert Dicosimo ,  Robert Donald Fallon

IPC分类号： C12N120

CPC分类号： C12N11/10 ,  C12N1/04 ,  C12N9/78 ,  C12N9/96 ,  C12N11/04 ,  C12N11/08 ,  C12P13/00

摘要： A method for preserving immobilized or unimmobilized microbial cells having nitrilase activity and for stabilizing the nitrilase activity of unimmobilized or immobilized microbial cells has been developed. The unimmobilized or immobilized microbial cells are stored in an aqueous solution containing from about 0.10 M to the saturation concentration of an inorganic salt of bicarbonate or carbonate, including ammonium, sodium and potassium salts of bicarbonate or carbonate. Aqueous suspensions containing at least 100 mM bicarbonate or carbonate limit microbial contamination of the stored enzyme catalyst, as well as stabilize the desired nitrilase activity of the uninmmobilized or immobilized cells. Microorganisms which are characterized by an nitrilase activity and are stabilized and preserved by this method include Acidovorax facilis 72-PF-15 (ATCC 55747), Acidovorax facilis 72-PF-17 (ATCC 55745), Acidovorax facilis 72W (ATCC 55746), and transformed microbial cells having nitrilase activity, preferably E. coli SS1001 (ATCC PTA-1177) which is transformed with Acidovorax facilis 72W nitrilase activity.

摘要翻译： 已经开发了用于保存具有腈水解酶活性的固定或未固化的微生物细胞并稳定未固定或固定的微生物细胞的腈水解酶活性的方法。 将未固定或固定的微生物细胞储存在含有约0.10M的水溶液中，使饱和浓度为碳酸氢盐或碳酸盐的无机盐，包括碳酸氢盐或碳酸盐的铵，钠和钾盐。 含有至少100mM碳酸氢盐或碳酸氢盐的水性悬浮液限制所储存的酶催化剂的微生物污染物，以及稳定未固定化或固定的细胞的所需腈水解酶活性。 其特征在于腈水解酶活性并通过该方法稳定和保存的微生物包括Acidovorax facilis 72-PF-15（ATCC 55747），Acidovorax facilis 72-PF-17（ATCC 55745），Acidovorax facilis 72W（ATCC 55746）和 转化的具有腈水解酶活性的微生物细胞，优选用Acidovorax facilis 72W腈水解酶活性转化的大肠杆菌SS1001（ATCC PTA-1177）。

公开(公告)号：US08003356B2

公开(公告)日：2011-08-23

申请号：US11105259

申请日：2005-04-13

申请人： Arie Ben-Bassat ,  Fateme Sima Sariaslani ,  Lisa L. Huang ,  Ranjan Patnaik ,  David J. Lowe

发明人： Arie Ben-Bassat ,  Fateme Sima Sariaslani ,  Lisa L. Huang ,  Ranjan Patnaik ,  David J. Lowe

IPC分类号： C12P13/22 ,  C12P7/40 ,  C12N1/21

CPC分类号： C12P7/42 ,  C12P7/40

摘要： Methods for the microbial production of para-hydroxycinnamic acid (pHCA) and cinnamic acid (CA) are provided. Microbes producing either tyrosine or phenylalanine are grown in the presence of either tyrosine ammonium lyase or phenylalanine ammonium lyase respectively where some part of the fermentation is accomplished at alkaline pH. The process results in greater yields and higher rates of para-hydroxycinnamic acid (pHCA) and cinnamic acid (CA) production as compared with fermentation exclusively at physiological pH.

摘要翻译： 提供了对羟基肉桂酸（pHCA）和肉桂酸（CA）的微生物生产方法。 生产酪氨酸或苯丙氨酸的微生物分别在酪氨酸裂解酶或苯丙氨酸铵裂解酶的存在下生长，其中部分发酵在碱性pH下完成。 与仅在生理pH下的发酵相比，该方法产生较高的产率和较高的对羟基肉桂酸（PHCA）和肉桂酸（CA）产率。

公开(公告)号：US06329192B1

公开(公告)日：2001-12-11

申请号：US08070650

申请日：1993-06-01

申请人： Arie Ben-Bassat ,  Robert Bruner ,  Sharon Shoemaker ,  Yehoshua Aloni ,  Harry Wong ,  Donald C. Johnson ,  Amar N. Neogi

发明人： Arie Ben-Bassat ,  Robert Bruner ,  Sharon Shoemaker ,  Yehoshua Aloni ,  Harry Wong ,  Donald C. Johnson ,  Amar N. Neogi

IPC分类号： C12N100

CPC分类号： C12R1/02 ,  A61L15/28 ,  C12P19/04 ,  Y10S435/823 ,  C08L1/02

摘要： A method and media for producing bacterial cellulose under agitated culture conditions resulting in sustained production over an average of 70 hours of at least 0.1 g/liter per hour are achieved. A unique reticulated cellulose product is produced using the methods and conditions claimed, and may be converted to of a sheet characterized by substantial resistance to densification and great tensile strength when produced by sheet forming means. Acetobacter strains are identified as stable under agitated culture conditions and exhibit substantially reduced gluconic and keto-gluconic acids production.

摘要翻译： 在搅拌培养条件下生产细菌纤维素的方法和介质，得到平均70小时至少0.1g /升/小时的持续生产。 使用所要求保护的方法和条件生产独特的网状纤维素产品，并且可以转化为由片材成型装置生产时具有显着的致密性和大的拉伸强度的片材。 醋酸杆菌菌株在搅拌培养条件下被鉴定为稳定的，并显示出显着降低的葡萄糖酸和酮 - 葡萄糖酸的产生。

公开(公告)号：US5821109A

公开(公告)日：1998-10-13

申请号：US442597

申请日：1995-05-17

申请人： Arie Ben-Bassat ,  Robert Bruner ,  Sharon Shoemaker ,  Yehoshua Aloni ,  Harry Wong ,  Donald C. Johnson ,  Amar N. Neogi

发明人： Arie Ben-Bassat ,  Robert Bruner ,  Sharon Shoemaker ,  Yehoshua Aloni ,  Harry Wong ,  Donald C. Johnson ,  Amar N. Neogi

IPC分类号： A61L15/28 ,  C12P19/04 ,  C12N1/00 ,  C12N1/20 ,  C12P1/04

CPC分类号： C12R1/02 ,  A61L15/28 ,  C12P19/04 ,  Y10S435/823

摘要： A method and media for producing bacterial cellulose under agitated culture conditions resulting in sustained production over an average of 70 hours of at least 0.1 g/liter per hour are achieved. A unique reticulated cellulose II product is produced using the methods and conditions claimed, and may be in the form of a sheet characterized by resistance to densification and great tensile strength when produced by sheet forming means. Also strains of Acetobacter that are stable under agitated culture conditions and that exhibit substantially reduced gluconic and keto-gluconic acids production are described.

摘要翻译： 在搅拌培养条件下生产细菌纤维素的方法和介质，得到平均70小时至少0.1g /升/小时的持续生产。 使用所要求保护的方法和条件制备独特的网状纤维素II产品，并且可以是以片材形成装置生产时的抗致密化和抗拉强度特性的片材的形式。 还描述了在搅拌培养条件下稳定并显示出显着降低的葡糖酸和酮 - 葡萄糖酸生产的醋杆菌菌株。

公开(公告)号：US5759828A

公开(公告)日：1998-06-02

申请号：US309512

申请日：1994-09-20

申请人： Rony Tal ,  David H. Gelfand ,  Roger D. Calhoon ,  Arie Ben-Bassat ,  Moshe Benziman ,  Hing Cheung Wong

发明人： Rony Tal ,  David H. Gelfand ,  Roger D. Calhoon ,  Arie Ben-Bassat ,  Moshe Benziman ,  Hing Cheung Wong

IPC分类号： C07K14/195 ,  C07K14/41 ,  C12N1/21 ,  C12N9/10 ,  C12N9/16 ,  C12N9/88 ,  C12N15/09 ,  C12N15/52 ,  C12P19/04 ,  C12P21/02 ,  C12R1/02 ,  C12N15/63 ,  C12N15/70 ,  C12N15/74 ,  C12P1/04

CPC分类号： C12N9/16 ,  C12N15/52 ,  C12N9/1051 ,  C12N9/88 ,  C12P19/04

摘要： The present invention provides the nucleotide sequences of Acetobacter operons, cdg operons encoding genes for the biosynthesis and degradation of cyclic diguanosine monophosphate (c-di-GMP). Specifically, the nucleotide sequences and deduced amino acid sequences of 3 phosphodiesterases isozymes, 3 diguanylate cyclase isozymes, and 2 polypeptides of unidentified function are provided. Also provided for are various strains of microorganisms, including Acetobacter cells genetically manipulated so as to produce elevated and/or reduced levels of one or more cdg operon encoded proteins.

摘要翻译： 本发明提供了醋酸杆菌操纵子的核苷酸序列，编码用于生物合成和环状二磷酸腺苷（c-di-GMP）的降解的基因的cdg操纵子。 具体地，提供了3种磷酸二酯酶同工酶，3个二环酸酯环化酶同功酶和2个未鉴定功能的多肽的核苷酸序列和推导的氨基酸序列。 还提供了各种微生物菌株，包括遗传操纵的醋杆菌细胞，以便产生升高和/或降低的一种或多种cdg操纵子编码的蛋白质的水平。

公开(公告)号：US20110237485A1

公开(公告)日：2011-09-29

申请号：US13031598

申请日：2011-02-21

申请人： Mark Aaron Emalfarb ,  Irina Vladimirovna Solovjeva ,  Arie Ben-Bassat ,  Richard P. Burlingame ,  Vladimir Mikhaylovich Chernoglazov ,  Oleg Nicolaevich Ocounev ,  Philip T. Olson ,  Arkady Panteleimonovich Sinitsyn

发明人： Mark Aaron Emalfarb ,  Irina Vladimirovna Solovjeva ,  Arie Ben-Bassat ,  Richard P. Burlingame ,  Vladimir Mikhaylovich Chernoglazov ,  Oleg Nicolaevich Ocounev ,  Philip T. Olson ,  Arkady Panteleimonovich Sinitsyn

IPC分类号： C11D3/386 ,  C12N9/42

CPC分类号： D06P5/158 ,  C11D3/38645 ,  C11D11/0017 ,  C12N9/2437 ,  C12R1/645 ,  C12Y302/01004 ,  D06L4/40 ,  D06M16/003 ,  D06M2101/06 ,  D06P5/02

摘要： The subject invention relates to novel compositions of neutral and/or alkaline cellulase and methods for obtaining neutral and/or alkaline cellulase compositions from Chrysosporium cultures, in particular Chrysosporium lucknowense. This invention also provides mutants and methods of generating mutants of Chrysosporium capable of producing neutral and/or alkaline cellulase. This invention also relates to the genes encoding the enzymes comprising the neutral and/or alkaline cellulase composition. In addition, this invention provides methods of culturing Chrysosporium to produce neutral and/or alkaline cellulases. The neutral and/or alkaline cellulase compositions of the subject invention can be used in a variety of processes including stone washing of clothing, detergent processes, deinking, color brightening, depilling and biobleaching of paper and pulp and treatment of waste streams. The present invention also relates to the isolation and purification of cellulase enzymes, having glucanase and cellobiohydrolase activity, and useful for stonewashing applications.

摘要翻译： 本发明涉及中和和/或碱性纤维素酶的新型组合物和从Chrysosporium培养物，特别是Chrysosporium lucknowense获得中性和/或碱性纤维素酶组合物的方法。 本发明还提供了产生能够产生中性和/或碱性纤维素酶的Chrysosporium突变体的突变体和方法。 本发明还涉及编码包含中性和/或碱性纤维素酶组合物的酶的基因。 此外，本发明提供培养Chrysosporium以产生中性和/或碱性纤维素酶的方法。 本发明的中性和/或碱性纤维素酶组合物可以用于各种工艺，包括洗衣服，洗涤剂工艺，脱墨，增色，脱色和纸和纸浆的生物漂白以及处理废物流。 本发明还涉及具有葡聚糖酶和纤维二糖水解酶活性的纤维素酶的分离和纯化，并且可用于石洗应用。

公开(公告)号：US07378261B2

公开(公告)日：2008-05-27

申请号：US10824581

申请日：2004-04-14

申请人： Arie Ben-Bassat ,  Sharon L. Haynie ,  David J. Lowe ,  Lisa L. Huang

发明人： Arie Ben-Bassat ,  Sharon L. Haynie ,  David J. Lowe ,  Lisa L. Huang

IPC分类号： C12P7/22 ,  C12N9/88 ,  C12N5/10 ,  C12N1/21 ,  C12N15/00 ,  C12N11/00 ,  C12P21/06

CPC分类号： C12P7/22 ,  C12P7/42

摘要： A biocatalytic method for preparing para-hydroxystyrene from para-hydroxycinnamic acid is described. The method uses an enzyme source having para-hydroxycinnamic acid decarboxylase activity to catalyze the decarboxylation of para-hydroxycinnamic acid in a biphasic reaction medium to produce para-hydroxystyrene, which is extracted into the organic phase of the biphasic reaction medium. The method results in a high yield of para-hydroxystyrene due to the decreased exposure of the enzyme source to the inhibitory product. The product is readily recovered from the extractant, or may be chemically derivatized directly in the extractant before recovery.

摘要翻译： 描述了由对羟基肉桂酸制备对羟基苯乙烯的生物催化方法。 该方法使用具有对羟基肉桂酸脱羧酶活性的酶源来催化双相反应介质中对羟基肉桂酸的脱羧作用，产生对羟基苯乙烯，将其提取到双相反应介质的有机相中。 该方法由于酶源暴露于抑制产物而导致对羟基苯乙烯的高产率。 该产物容易从萃取剂中回收，或者可以在回收之前在萃取剂中直接化学衍生化。

公开(公告)号：US20050260724A1

公开(公告)日：2005-11-24

申请号：US11105259

申请日：2005-04-13

申请人： Arie Ben-Bassat ,  Fateme Sariaslani ,  Lisa Huang ,  Ranjan Patnaik ,  David Lowe

发明人： Arie Ben-Bassat ,  Fateme Sariaslani ,  Lisa Huang ,  Ranjan Patnaik ,  David Lowe

IPC分类号： C12N1/21 ,  C12P7/40 ,  C12P7/42 ,  C12P13/22

CPC分类号： C12P7/42 ,  C12P7/40

摘要： Methods for the microbial production of para-hydroxycinnamic acid (pHCA) and cinnamic acid (CA) are provided. Microbes producing either tyrosine or phenylalanine are grown in the presence of either tyrosine ammonium lyase or phenylalanine ammonium lyase respectively where some part of the fermentation is accomplished at alkaline pH. The process results in greater yields and higher rates of para-hydroxycinnamic acid (pHCA) and cinnamic acid (CA) production as compared with fermentation exclusively at physiological pH.

摘要翻译： 提供了对羟基肉桂酸（pHCA）和肉桂酸（CA）的微生物生产方法。 生产酪氨酸或苯丙氨酸的微生物分别在酪氨酸裂解酶或苯丙氨酸铵裂解酶的存在下生长，其中部分发酵在碱性pH下完成。 与仅在生理pH下的发酵相比，该方法产生较高的产率和较高的对羟基肉桂酸（PHCA）和肉桂酸（CA）产率。

公开(公告)号：US07348421B1

公开(公告)日：2008-03-25

申请号：US10754648

申请日：2004-01-12

申请人： Juan-Luis Ramos ,  Patricia Godoy-Alba ,  Arie Ben-Bassat ,  Maria-Isabel Ramos-Gonzalez ,  Estrella Duque

发明人： Juan-Luis Ramos ,  Patricia Godoy-Alba ,  Arie Ben-Bassat ,  Maria-Isabel Ramos-Gonzalez ,  Estrella Duque

IPC分类号： C07H21/04 ,  C12N1/21 ,  C12N9/00

CPC分类号： C07K14/21 ,  C12N15/52 ,  C12P7/42

摘要： This invention relates to the isolation of a novel tonB operon from Pseudomonas putida. These genes are useful to render the cells more sensitive to antibiotics, toluene, pHBA, aromatic compounds, parabenes, and aromatic amino acids after inactivation with specific mutant allels or more tolerant to these compounds after overexpression with appropriate expression vector. These findings are important in the field of medicine and biotechnology and biocatalysis. In addition a screen to identify pHBA tolerant genes is provided and strains with significant tolerance to pHBA were identified. These strains are important for pHBA production.

摘要翻译： 本发明涉及从恶臭假单胞菌中分离出一种新的tonB操纵子。 在用适当的表达载体过表达后，这些基因可用于使细胞在特异性突变体等位基因失活后对抗生素，甲苯，pHBA，芳香族化合物，对羟基苯甲酸和芳香族氨基酸更敏感或更耐受这些化合物。 这些发现在医学和生物技术和生物催化领域是重要的。 另外还提​​供了一个识别pHBA耐受基因的筛选条件，并鉴定出对pHBA具有显着耐受性的菌株。 这些菌株对pHBA生产很重要。

公开(公告)号：US20060246559A1

公开(公告)日：2006-11-02

申请号：US11484129

申请日：2006-07-11

申请人： Arie Ben-Bassat ,  Monica Cattermole ,  Anthony Gatenby ,  Katharine Gibson ,  M. Ramos-Gonzalez ,  Juan Ramos ,  Sima Sariaslani

发明人： Arie Ben-Bassat ,  Monica Cattermole ,  Anthony Gatenby ,  Katharine Gibson ,  M. Ramos-Gonzalez ,  Juan Ramos ,  Sima Sariaslani

IPC分类号： C12P7/62 ,  C12P7/40

CPC分类号： C12P7/62 ,  C12N15/52 ,  C12P7/42

摘要： Bacterial strains transformed with the pcu genes are useful for the production of para-hydroxybenzoate (PHBA). Applicant has provided the p-cresol utilizing (pcu) and tmoX gene sequences from Pseudomonas mendocina KR-1, the proteins encoded by these sequences, recombinant plasmids containing such sequences, and bacterial host cells containing such plasmids or integrated sequences. Method for the use of these materials to produce PHBA are also disclosed.

摘要翻译： 用pcu基因转化的细菌菌株可用于生产对羟基苯甲酸酯（PHBA）。 申请人提供了使用来自假单胞菌KR-1的对甲酚（pcu）和tmoX基因序列，由这些序列编码的蛋白质，含有此类序列的重组质粒和含有此类质粒或整合序列的细菌宿主细胞。 还公开了使用这些材料制备PHBA的方法。