公开(公告)号：US20180291444A1

公开(公告)日：2018-10-11

申请号：US15935897

申请日：2018-03-26

Applicant: ILLUMINA, INC.

Inventor： Hongxia Xu ,  Alex Aravanis ,  Shengrong Lin

IPC: C12Q1/6874 ,  C12Q1/6837 ,  C12N15/10

Abstract: The present disclosure relates to the field of molecular biology and more specifically to methods for capturing, amplifying and sequencing target polynucleotides on a solid surface.

公开(公告)号：US20170144155A1

公开(公告)日：2017-05-25

申请号：US15315638

申请日：2015-06-03

Applicant: ILLUMINA, INC.

Inventor： Sebastian Bohm ,  Alex Aravanis ,  Alexander Hsiao ,  Behnam Javanmardi ,  Tarun Khurana ,  Hai Quang Tran ,  Majid Aghababazadeh ,  M. Shane Bowen ,  Boyan Boyanov ,  Dale Buermann

IPC: B01L3/00 ,  C12Q1/68 ,  B01L7/00

Abstract: Systems and methods for conducting designated reactions that include a fluidic network having a sample channel, a reaction chamber, and a reservoir. The sample channel is in flow communication with a sample port. The system also includes a rotary valve that has a flow channel and is configured to rotate between first and second valve positions. The flow channel fluidically couples the reaction chamber and the sample channel when the rotary valve is in the first valve position and fluidically couples the reservoir and the reaction chamber when the rotary valve is in the second valve position. A pump assembly induces a flow of a biological sample toward the reaction chamber when the rotary valve is in the first valve position and induces a flow of a reaction component from the reservoir toward the reaction chamber when the rotary valve is in the second valve position.

公开(公告)号：US11786898B2

公开(公告)日：2023-10-17

申请号：US16818953

申请日：2020-03-13

Applicant: Illumina, Inc.

Inventor： Sebastian Bohm ,  Alex Aravanis ,  Alexander Hsiao ,  Behnam Javanmardi ,  Tarun Khurana ,  Hai Quang Tran ,  Majid Aghababazadeh ,  M. Shane Bowen ,  Boyan Boyanov ,  Dale Buermann

IPC: B01L3/00 ,  B01L7/00 ,  F16K99/00 ,  G01N35/08 ,  C12Q1/6869

CPC classification number: B01L3/502738 ,  B01L3/50273 ,  B01L3/502715 ,  B01L7/52 ,  C12Q1/6869 ,  F16K99/0013 ,  F16K99/0015 ,  F16K99/0028 ,  G01N35/08 ,  B01L2200/10 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2400/0487 ,  B01L2400/0644 ,  B01L2400/0655 ,  F16K2099/0084

Abstract: Systems and methods for conducting designated reactions that include a fluidic network having a sample channel, a reaction chamber, and a reservoir. The sample channel is in flow communication with a sample port. The system also includes a rotary valve that has a flow channel and is configured to rotate between first and second valve positions. The flow channel fluidically couples the reaction chamber and the sample channel when the rotary valve is in the first valve position and fluidically couples the reservoir and the reaction chamber when the rotary valve is in the second valve position. A pump assembly induces a flow of a biological sample toward the reaction chamber when the rotary valve is in the first valve position and induces a flow of a reaction component from the reservoir toward the reaction chamber when the rotary valve is in the second valve position.

公开(公告)号：US20230145389A1

公开(公告)日：2023-05-11

申请号：US18087499

申请日：2022-12-22

Applicant: ILLUMINA, INC.

Inventor： Alex Aravanis ,  Boyan Boyanov ,  M. Shane Bowen ,  Dale Buermann ,  Alexander Hsiao ,  Behnam Javanmardi ,  Tarun Khurana ,  Poorya Sabounchi ,  Hai Quang Tran

IPC: B01L3/00 ,  F16K99/00 ,  B01L7/00

CPC classification number: B01L3/502715 ,  B01L3/502738 ,  B01L7/525 ,  F16K99/0013 ,  F16K99/0015 ,  F16K99/0028 ,  B01L2200/04 ,  B01L2200/10 ,  B01L2200/16 ,  B01L2200/025 ,  B01L2200/027 ,  B01L2200/0689 ,  B01L2300/0663 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2300/0883 ,  B01L2300/0887 ,  B01L2300/1805 ,  B01L2300/1827 ,  B01L2400/065 ,  B01L2400/0633 ,  B01L2400/0638 ,  B01L2400/0644 ,  B01L2400/0655 ,  F16K2099/0074 ,  F16K2099/0084

Abstract: Systems and methods for conducting designated reactions utilizing a base instrument and a removable cartridge. The removable cartridge includes a fluidic network that receives and fluidically directs a biological sample to conduct the designated reactions. The removable cartridge also includes a flow-control valve that is operably coupled to the fluidic network and is movable relative to the fluidic network to control flow of the biological sample therethrough. The removable cartridge is configured to separably engage a base instrument. The base instrument includes a valve actuator that engages the flow-control valve of the removable cartridge. A detection assembly held by at least one of the removable cartridge or the base instrument may be used to detect the designated reactions.

公开(公告)号：US20200165650A1

公开(公告)日：2020-05-28

申请号：US16659475

申请日：2019-10-21

Applicant: Illumina, Inc.

Inventor： Gordon M. Cann ,  Jeffrey G. Mandell ,  Alex Aravanis ,  Steven Norberg ,  Dmitry K. Pokholok ,  Frank J. Steemers ,  Farnaz Absalan ,  Leila Bazargan

IPC: C12P19/34 ,  C12Q1/683 ,  C12Q1/6869 ,  C12Q1/6816 ,  C12N15/10

Abstract: A method for enriching a target nucleic acid comprising providing an endonuclease system having a crRNA or a derivative thereof, and a Cas protein or a variant thereof. The crRNA or the derivative thereof contains a target-specific nucleotide region substantially complementary to a region of the target nucleic acid; contacting the target nucleic acid with the endonuclease system to form a complex; and separating the complex and thereby enriching for the target nucleic acid.

公开(公告)号：US20160319345A1

公开(公告)日：2016-11-03

申请号：US15130668

申请日：2016-04-15

Applicant: Illumina, Inc.

Inventor： Sante Gnerre ,  Byoungsok Jung ,  Emrah Kostem ,  Alex Aravanis ,  Alex So ,  Xuyu Cai ,  Zhihong Zhang

IPC: C12Q1/68 ,  C12N15/10 ,  G06F19/22

CPC classification number: C12Q1/6869 ,  C12N15/1065 ,  C12Q1/6806 ,  C12Q1/6855 ,  G16B30/00 ,  C12Q2525/191 ,  C12Q2535/119 ,  C12Q2535/122 ,  C12Q2563/179 ,  C12Q2565/514

Abstract: The disclosed embodiments concern methods, apparatus, systems and computer program products for determining sequences of interest using unique molecular index (UMI) sequences that are uniquely associable with individual polynucleotide fragments, including sequences with low allele frequencies and long sequence length. In some implementations, the UMIs include both physical UMIs and virtual UMIs. In some implementations, the unique molecular index sequences include non-random sequences. System, apparatus, and computer program products are also provided for determining a sequence of interest implementing the methods disclosed.

Abstract translation: 所公开的实施例涉及使用与单个多核苷酸片段唯一相关联的独特分子指数（UMI）序列确定感兴趣序列的方法，装置，系统和计算机程序产品，包括具有低等位基因频率和长序列长度的序列。 在一些实现中，UMI包括物理UMI和虚拟UMI。 在一些实施方案中，独特的分子索引序列包括非随机序列。 还提供了系统，装置和计算机程序产品，用于确定实现所公开方法的感兴趣的顺序。

公开(公告)号：US20160199832A1

公开(公告)日：2016-07-14

申请号：US14912394

申请日：2014-08-29

Applicant: ILLUMINA, INC. ,  ADVANCED LIQUID LOGIC FRANCE SAS

Inventor： Arash Jamshidi ,  Yan-You Lin ,  Alex Aravanis ,  Cyril Delattre ,  Arnaud Rival ,  Jennifer Foley ,  Poorya Sabounchi ,  Tarun Khurana ,  Majid Babazadeh ,  Hamed Amini ,  Bala Murali Venkatesan ,  M. Shane Bowen ,  Steven M. Barnard ,  Maria Candelaria Rogert Bacigalupo ,  Dietrich Dehlinger

IPC: B01L3/00

CPC classification number: B01L3/50273 ,  B01F13/0071 ,  B01F13/0076 ,  B01F13/0084 ,  B01L3/502792 ,  B01L2200/06 ,  B01L2300/0636 ,  B01L2300/0819 ,  B01L2300/0858 ,  B01L2300/087 ,  B01L2300/161 ,  B01L2300/166 ,  B01L2400/0427 ,  B41J2/04 ,  G01N15/1463 ,  G01N15/1475 ,  G01N2015/1006

Abstract: Provided herein is a droplet actuator including (a) first and second substrates separated by a droplet-operations gap, the first and second substrates including respective hydrophobic surfaces that face the droplet-operations gap; (b) a plurality of electrodes coupled to at least one of the first substrate and the second substrate, the electrodes arranged along the droplet-operations gap to control movement of a droplet along the hydrophobic surfaces within the droplet-operations gap; and (c) a hydrophilic or variegated-hydrophilic surface exposed to the droplet-operations gap, the hydrophilic or variegated-hydrophilic surface being positioned to contact the droplet when the droplet is at a select position within the droplet-operations gap.

Abstract translation: 本文提供的液滴致动器包括（a）由液滴操作间隙分离的第一和第二基板，第一和第二基板包括面对液滴操作间隙的各个疏水表面; （b）耦合到第一衬底和第二衬底中的至少一个的多个电极，电极沿着液滴操作间隙排列，以控制液滴沿着液滴操作间隙内的疏水表面的运动; 和（c）暴露于液滴操作间隙的亲水或杂色亲水表面，当液滴在液滴操作间隙内的选择位置时，亲水或杂色亲水表面定位成接触液滴。