中科微点-全球专利检索

  1. Login
  2. Sign Up

Search Results

61 records (took 0.128 seconds)

    Affinity chromatography matrix
    33.
    发明授权
    Affinity chromatography matrix 有权

    公开(公告)号：US10189891B2

    公开(公告)日：2019-01-29

    申请号：US14385336

    申请日：2013-03-26

    Applicant: GE Healthcare BioProcess R&D AB

    Inventor： Mats Ander ,  Goran Bauren ,  Tomas Bjorkman ,  Per-Mikael Aberg ,  Gustav Rodrigo

    IPC: C07K1/22 ,  C07K14/31 ,  C07K16/00 ,  B01J20/286 ,  B01D15/38 ,  B01J20/291 ,  G01N33/68 ,  B01J20/32

    Abstract: The invention discloses an immunoglobulin-binding protein comprising one or more mutated immunoglobulin-binding domains (monomers) of staphylococcal Protein A (E, D, A, B, C) or protein Z or a functional variant thereof, wherein in at least one of the one or more mutated monomers, the asparagine or histidine at the position corresponding to H18 of the B domain of Protein A or of Protein Z has been deleted or substituted with a first amino acid residue which is not proline or asparagine and wherein, if the amino acid residue at position 57 is proline and the amino acid residue at position 28 is asparagine, then the amino acid residue at the position corresponding to H18 of the B domain of protein A or of protein Z is not serine, threonine or lysine.

    Parallel assembly of chromatography column modules
    34.
    发明授权
    Parallel assembly of chromatography column modules 有权

    公开(公告)号：US10092856B2

    公开(公告)日：2018-10-09

    申请号：US15793923

    申请日：2017-10-25

    Applicant: GE Healthcare BioProcess R&D AB

    Inventor： Klaus Gebauer

    IPC: B01D15/14 ,  B01D15/18 ,  B01D15/22 ,  G01N30/60 ,  G01N30/46 ,  G01N30/88

    Abstract: A parallel assembly (2; 11; 51) of chromatography column modules (3a,b,c; 13a,b,c; 53a,b,c, 90a, b), the assembly having one common assembly inlet (15; 55) and one common assembly outlet (17; 57), each column module comprising a bed space (29) filled with chromatography medium and each column module comprises integrated fluid conduits which when the column module is connected with other column modules are adapted to connect the bed space (29) of the column module with the assembly inlet (15; 55) and the assembly outlet (17; 57), wherein the total length and/or volume of the fluid conduit from the assembly inlet to one bed space together with the length and/or volume of the fluid conduit from the same bed space to the assembly outlet is substantially the same for all bed spaces and modules installed in the parallel assembly.

    Method for Endotoxin Removal
    38.
    发明申请
    Method for Endotoxin Removal 审中-公开
    内毒素去除方法

    公开(公告)号：US20170067031A1

    公开(公告)日：2017-03-09

    申请号：US15355675

    申请日：2016-11-18

    Applicant: GE Healthcare BioProcess R&D AB

    Inventor： Patrik Adielsson ,  Tobias E. Soderman ,  Anna Akerblom

    IPC: C12N7/00 ,  B01D15/38

    CPC classification number: C12N7/00 ,  B01D15/3847 ,  C12N2760/16151

    Abstract: The present invention relates to a method for endotoxin removal from a sample comprising the following steps: combining the sample comprising one or more target molecule(s) with a chromatography media comprising beads having an inner porous core functionalized with ligands capable of binding endotoxin and an outer porous layer without functional groups and a pore size small enough to exclude the target molecule from the inner core; and collecting the sample from the media, wherein the sample comprises an endotoxin level which is at least 75% less, preferably 90% less, than before the removal and the yield of the target molecule is at least 75%.

    Abstract translation: 本发明涉及一种从样品中去除内毒素的方法，包括以下步骤：将包含一种或多种靶分子的样品与包含珠的色谱介质组合，所述珠粒具有由能够结合内毒素的配体功能化的内部多孔核心和 没有官能团的外部多孔层和足够小以从靶芯排出靶分子的孔径; 并从培养基中收集样品，其中样品包含与去除前相比至少75％以上，优选90％以下的内毒素水平，并且目标分子的产率为至少75％。

    Mutated immunoglobulin-binding protein
    39.
    发明授权
    Mutated immunoglobulin-binding protein 有权
    突变的免疫球蛋白结合蛋白

    公开(公告)号：US09534023B2

    公开(公告)日：2017-01-03

    申请号：US14845817

    申请日：2015-09-04

    Applicant: GE Healthcare Bio-Sciences AB

    Inventor： Sophia Hober

    IPC: C07K1/22 ,  C07K14/195 ,  C07K14/31 ,  B01D15/38 ,  B01J20/286 ,  B01J20/32 ,  C07K16/06

    CPC classification number: B01D15/3809 ,  B01J20/24 ,  B01J20/286 ,  B01J20/289 ,  B01J20/3244 ,  B01J2220/44 ,  B01J2220/52 ,  C07K1/22 ,  C07K14/001 ,  C07K14/195 ,  C07K14/31 ,  C07K16/065 ,  Y10S530/81 ,  Y10S530/825

    Abstract: The present invention relates to an immunoglobulin-binding protein, wherein at least one asparagine residue has been mutated to an amino acid other than glutamine or aspartic acid, which mutation confers an increased chemical stability at pH-values of up to about 13-14 compared to the parental molecule. The protein can for example be derived from a protein capable of binding to other regions of the immunoglobulin molecule than the complementarity determining regions (CDR), such as protein A, and preferably the B-domain of Staphylococcal protein A. The invention also relates to a matrix for affinity separation, which comprises an immunoglobulin-binding protein as ligand coupled to a solid support, in which protein ligand at least one asparagine residue has been mutated to an amino acid other than glutamine.

    Abstract translation: 本发明涉及一种免疫球蛋白结合蛋白，其中至少一种天冬酰胺残基已被突变为除谷氨酰胺或天冬氨酸以外的氨基酸，该突变赋予pH值高达约13-14的化学稳定性增加 到亲本分子。 蛋白质可以例如衍生自能够与互补决定区（CDR）如蛋白A，优选葡萄球菌蛋白A的B结构域结合的免疫球蛋白分子的其它区域的蛋白质。本发明还涉及 用于亲和力分离的基质，其包含免疫球蛋白结合蛋白作为与固体支持物偶联的配体，其中蛋白质配体至少一种天冬酰胺残基已被突变为除谷氨酰胺以外的氨基酸。

Patent Agency Ranking