公开(公告)号：US11933785B2

公开(公告)日：2024-03-19

申请号：US16307779

申请日：2017-06-12

申请人： Wisconsin Alumni Research Foundation

发明人： Michael Richard Sussman ,  Melanie Mae Ivancic ,  Gregory D Kennedy ,  William Franklin Dove ,  Perry Joseph Pickhardt ,  Mark Reichelderfer

IPC分类号： G01N31/00 ,  G01N33/53 ,  G01N33/574

CPC分类号： G01N33/57419 ,  G01N2333/4704 ,  G01N2333/4716 ,  G01N2333/4728 ,  G01N2333/4737 ,  G01N2333/70585 ,  G01N2333/71 ,  G01N2333/7456 ,  G01N2333/81 ,  G01N2333/90212 ,  G01N2333/90283 ,  G01N2800/60

摘要： This invention provides minimally invasive methods, reagents, diagnostic and prognostic markers useful for staging, prognosis and surveillance of colorectal cancers, and therapeutic intervention in individuals with colorectal cancers, or individuals who may be susceptible to developing colorectal cancers.

公开(公告)号：US11726088B2

公开(公告)日：2023-08-15

申请号：US16991429

申请日：2020-08-12

申请人： THE TRUSTEES OF PRINCETON UNIVERSITY

发明人： Thomas Shenk ,  Todd M. Greco ,  Ileana M. Cristea ,  Rommel A. Mathias ,  Adam Oberstein

IPC分类号： C12Q1/34 ,  G01N33/573

CPC分类号： G01N33/573 ,  C12Q1/34 ,  G01N2333/90212 ,  G01N2333/91057 ,  G01N2500/00

摘要： The present application provides a method of assaying pyruvate dehydrogenase complex (PDHC) activity in a mammalian cell that expresses human sirtuin 4 (SIRT4) comprising measuring a level of a dihydrolipoyllysine acetyltransferase (DLAT) lipoamide peptide comprising the amino acid sequence TDK[lipoyl]AT in the cell. The present application also demonstrates that sirtuin 4 (SIRT4) acts as a cellular lipoamidase that negatively regulates pyruvate dehydrogenase complex (PDHC) activity through hydrolysis of its lipoamide cofactors.

公开(公告)号：US20190094225A1

公开(公告)日：2019-03-28

申请号：US15106932

申请日：2015-01-15

申请人： The Trustees of Princeton University

发明人： Thomas Shenk ,  Todd M. Greco ,  IIeana M. Cristea ,  Rommel A. Mathias ,  Adam Oberstein

IPC分类号： G01N33/573

CPC分类号： G01N33/573 ,  C12Q1/34 ,  G01N2333/90212 ,  G01N2333/91057 ,  G01N2500/00

摘要： The present application demonstrates that sirtuin 4 (SIRT4) acts as a cellular lipoamidase that negatively regulates pyruvate dehydrogenase complex (PDHC) activity through hydrolysis of its lipoamide cofactors.

公开(公告)号：US20180305377A1

公开(公告)日：2018-10-25

申请号：US16024454

申请日：2018-06-29

申请人： EWHA UNIVERSITY-INDUSTRY COLLABORATION FOUNDATION

发明人： Sang Won KANG ,  Dong Hoon KANG ,  Doo Jae LEE

IPC分类号： C07D513/08 ,  A61K31/548 ,  A61K31/00 ,  C12Q1/26

CPC分类号： C07D513/08 ,  A61K31/00 ,  A61K31/548 ,  C12Q1/26 ,  G01N2333/90212 ,  G01N2500/02

摘要： The present invention relates to an epidithiodioxopiperazine derivative represented by the following Chemical Formula 1 or its reduced derivative; a method for preparing a compound represented by Chemical Formula 1 having improved intracellular permeability and mimicking the activity of 2-Cys-Prx in its reduced form in the cells; a pharmaceutical composition for preventing or treating vascular diseases comprising an epidithiodioxopiperazine compound or its derivatives or pharmaceutically acceptable salts thereof as an active ingredient; a drug delivery device for local administration including the pharmaceutical composition; and a pharmaceutical composition for inhibiting melanoma metastasis comprising the epidithiodioxopiperazine compound or its derivatives or pharmaceutically acceptable salts thereof as an active ingredient.

公开(公告)号：US09765090B2

公开(公告)日：2017-09-19

申请号：US14286929

申请日：2014-05-23

申请人： EWHA UNIVERSITY-INDUSTRY COLLABORATION FOUNDATION

发明人： Sang Won Kang ,  Dong Hoon Kang ,  Doo Jae Lee

IPC分类号： A61K31/54 ,  C07D513/08 ,  C12Q1/26 ,  A61K31/00

CPC分类号： A61K31/548 ,  A61K31/00 ,  C07D513/08 ,  C12Q1/26 ,  G01N2333/90212 ,  G01N2500/02

摘要： The present invention relates to an epidithiodioxopiperazine derivative represented by the following Chemical Formula 1 or its reduced derivative; a method for preparing a compound represented by Chemical Formula 1 having improved intracellular permeability and mimicking the activity of 2-Cys-Prx in its reduced form in the cells; a pharmaceutical composition for preventing or treating vascular diseases comprising an epidithiodioxopiperazine compound or its derivatives or pharmaceutically acceptable salts thereof as an active ingredient; a drug delivery device for local administration including the pharmaceutical composition; and a pharmaceutical composition for inhibiting melanoma metastasis comprising the epidithiodioxopiperazine compound or its derivatives or pharmaceutically acceptable salts thereof as an active ingredient.

公开(公告)号：US20160304932A1

公开(公告)日：2016-10-20

申请号：US15087842

申请日：2016-03-31

申请人： Anhui Agricultural University

发明人： Jinsong Zhang ,  Kang Sun

IPC分类号： C12Q1/26 ,  C12Q1/28

CPC分类号： C12Q1/26 ,  G01N2333/90212 ,  G01N2800/085 ,  G01N2800/26 ,  G01N2800/7028

摘要： The present invention refers to a method and a kit for the diagnosis of a hepatic disease utilizing the sulfydryl-oxidizing property of serum. Sulfydryl can be oxidized by serum, due to the QSOXs and other macromolecules or small molecules in serum. The specific activity of QSOXs in serum can be detected by fluorescent quantification using Amplex UltraRed (AUR), horseradish perioxidase, dithiothreitol, and tween as key reagents. The total dithiol oxidated capacity of the serum can be detected by colorimetric quantification using dithiothreitol, dithio-bis-nitrobenzoic acid, and guanidine hydrochloride as key reagents. The present invention shows that the specific activity of QSOXs in serum and the total dithiol oxidated capacity of the serum can be used for the diagnosis of hepatic disease, wherein the total dithiol oxidated capacity of the serum shows better diagnostic effect. The present invention also refers to a kit for the diagnosis of a hepatic disease, comprising a key reagent including phosphate buffer, Amplex UltraRed (AUR), horseradish perioxidase, dithiothreitol, and tween; or including guanidine hydrochloride, dithio-bis-nitrobenzoic acid, and dithiothreitol, etc.

摘要翻译： 本发明涉及利用血清的硫氧化 - 氧化性质来诊断肝脏疾病的方法和试剂盒。 由于QSOX和其他大分子或血清中的小分子，Sulfydryl可被血清氧化。 通过使用Amplex UltraRed（AUR），辣根过氧化酶，二硫苏糖醇和吐温作为关键试剂进行荧光定量，可以检测QSOX在血清中的比活性。 可以通过使用二硫苏糖醇，二硫代双硝基苯甲酸和盐酸胍作为关键试剂进行比色定量检测血清总二硫醇氧化能力。 本发明显示，血清中QSOXs的比活性和血清二硫醇总氧化能力可用于诊断肝脏疾病，其中血清总二硫醇氧化能力显示更好的诊断效果。 本发明还涉及用于诊断肝病的试剂盒，其包括磷酸盐缓冲液，Amplex UltraRed（AUR），辣根过氧化酶，二硫苏糖醇和吐温的关键试剂; 或包括盐酸胍，二硫代双硝基苯甲酸和二硫苏糖醇等。

公开(公告)号：US20150250808A1

公开(公告)日：2015-09-10

申请号：US14434931

申请日：2013-10-15

申请人： Vojo P. DERETIC ,  Eliseo F. CASTILLO ,  STC.UNM

发明人： Vojo P. Deretic ,  Eliseo F. Castillo

IPC分类号： A61K31/704 ,  A61K31/4184 ,  A61K31/473 ,  A61K31/495 ,  A61K31/4365 ,  A61K31/472 ,  A61K31/4535 ,  A61K31/65 ,  A61K31/4453 ,  A61K31/5415 ,  A61K31/277 ,  A61K31/38 ,  A61K31/475 ,  A61K31/57 ,  A61K38/17 ,  C07K16/28 ,  A61K31/381 ,  A61K31/436 ,  A61K31/506 ,  C07K16/24 ,  G01N33/68 ,  G01N33/569 ,  A61K45/06

CPC分类号： A61K31/704 ,  A61K9/0073 ,  A61K31/00 ,  A61K31/277 ,  A61K31/38 ,  A61K31/381 ,  A61K31/4184 ,  A61K31/436 ,  A61K31/4365 ,  A61K31/4453 ,  A61K31/4535 ,  A61K31/472 ,  A61K31/473 ,  A61K31/475 ,  A61K31/495 ,  A61K31/506 ,  A61K31/5415 ,  A61K31/57 ,  A61K31/65 ,  A61K31/713 ,  A61K38/00 ,  A61K38/177 ,  A61K38/1793 ,  A61K45/06 ,  C07K16/245 ,  C07K16/28 ,  C07K2317/76 ,  G01N33/5055 ,  G01N33/5695 ,  G01N33/6842 ,  G01N33/6893 ,  G01N2333/4703 ,  G01N2333/4719 ,  G01N2333/4724 ,  G01N2333/54 ,  G01N2333/545 ,  G01N2333/90212 ,  G01N2500/02 ,  G01N2500/20

摘要： In one embodiment, the invention provides a method of treating a subject suffering from a Mycobacterium infection by administering to the subject a therapeutically-effective amount of a degradative autophagy agonist or a secretory autophagy antagonist. In another embodiment, the invention provides a method of treating a subject suffering from one or more diseases selected from the group consisting of a Mycobacterium infection, an inflammatory disorder, an immune disorder, a cancer and a neurodegenerative disorder by administering to the subject a therapeutically-effective amount of a TBK-1 antagonist (e.g. BX795 or amlexanox). Related pharmaceutical compositions, diagnostic and screening assays and kits are also provided.

摘要翻译： 在一个实施方案中，本发明提供了通过向受试者施用治疗有效量的降解性自噬激动剂或分泌型自噬拮抗剂来治疗患有分枝杆菌感染的受试者的方法。 在另一个实施方案中，本发明提供了一种治疗患有一种或多种疾病的受试者的治疗方法，所述疾病选自由分枝杆菌感染，炎症性疾病，免疫病症，癌症和神经变性疾病组成的组， - 有效量的TBK-1拮抗剂（如BX795或amlexanox）。 还提供了相关的药物组合物，诊断和筛选测定和试剂盒。

公开(公告)号：US20130196342A1

公开(公告)日：2013-08-01

申请号：US13094091

申请日：2011-04-26

申请人： Jonathan S. Stamler ,  Limin Liu

发明人： Jonathan S. Stamler ,  Limin Liu

IPC分类号： G01N33/573

CPC分类号： G01N33/573 ,  A01K67/0276 ,  A01K2217/075 ,  A01K2227/105 ,  A01K2267/035 ,  A61K31/155 ,  A61K31/41 ,  A61K38/44 ,  C12N9/0008 ,  C12N15/8509 ,  C12Q1/26 ,  C12Y102/01046 ,  G01N2333/90212 ,  G01N2800/00 ,  A61K2300/00

摘要： Disclosed herein are methods and compositions for modulating the levels and/or activity of S-nitrosoglutathione reductase (GSNOR) in vivo or in vitro. Specifically disclosed are GSNOR deletion constructs, host cells and non-human mammals comprising GSNOR deletions, and methods of screening employing GSNOR deletion mutants. Also specifically disclosed are reagents and procedures for measuring, monitoring, or altering GSNOR levels or activity (as well as nitric oxide and S-nitrosothiol levels) in connection with various medical conditions.

摘要翻译： 本文公开了用于在体内或体外调节S-亚硝基谷胱甘肽还原酶（GSNOR）的水平和/或活性的方法和组合物。 具体公开的是GSNOR缺失构建体，宿主细胞和包含GSNOR缺失的非人哺乳动物，以及使用GSNOR缺失突变体进行筛选的方法。 还具体公开了用于测量，监测或改变与各种医学状况有关的GSNOR水平或活性（以及一氧化氮和S-亚硝基硫醇水平）的试剂和程序。

公开(公告)号：US20120130195A1

公开(公告)日：2012-05-24

申请号：US12951652

申请日：2010-11-22

申请人： Stephanie M. Martin ,  Ronnie L. Phillips ,  SangWook Lee ,  JunMo Gil

发明人： Stephanie M. Martin ,  Ronnie L. Phillips ,  SangWook Lee ,  JunMo Gil

IPC分类号： A61B5/00

CPC分类号： A61B5/4337 ,  A61B5/14507 ,  A61B5/14546 ,  A61B5/14735 ,  A61B2562/0295 ,  A61F13/20 ,  C12Q1/28 ,  C12Q1/32 ,  G01N2333/90209 ,  G01N2333/90212 ,  G01N2333/90241 ,  G01N2333/904

摘要： A diagnostic kit for biomarkers of beneficial vaginal health is described. The kit includes a first detection zone within which a D-lactic acid detection signal is capable of being generated, wherein the presence or quantity of D-lactic acid, is determinable from the D-lactic acid detection signal. The kit further includes a second detection zone within which a hydrogen peroxide detection signal is capable of being generated, wherein the presence or quantity of hydrogen peroxide, is determinable from the hydrogen peroxide detection signal.

摘要翻译： 描述了有益于阴道健康的生物标志物的诊断试剂盒。 试剂盒包括能够产生D-乳酸检测信号的第一检测区域，其中D-乳酸的存在或数量可从D-乳酸检测信号确定。 试剂盒还包括其中能够产生过氧化氢检测信号的第二检测区，其中过氧化氢的存在或数量可从过氧化氢检测信号确定。

公开(公告)号：US20100159470A1

公开(公告)日：2010-06-24

申请号：US12714257

申请日：2010-02-26

申请人： Marcia Belvin ,  Helen Francis-Lang ,  Lori Friedman ,  Gregory D. Plowman ,  Timothy S. Heuer

发明人： Marcia Belvin ,  Helen Francis-Lang ,  Lori Friedman ,  Gregory D. Plowman ,  Timothy S. Heuer

IPC分类号： C12Q1/68

CPC分类号： G01N33/5011 ,  C12Q1/485 ,  C12Q1/527 ,  G01N33/5017 ,  G01N33/573 ,  G01N33/574 ,  G01N33/57415 ,  G01N33/57419 ,  G01N33/57423 ,  G01N33/57449 ,  G01N33/5748 ,  G01N33/57484 ,  G01N33/57496 ,  G01N33/6872 ,  G01N2333/82 ,  G01N2333/90212 ,  G01N2333/912 ,  G01N2333/988 ,  G01N2500/00 ,  G01N2500/10 ,  G01N2510/00

摘要： Human SCD genes are identified as modulators of the p53 pathway, and thus are therapeutic targets for disorders associated with defective p53 function. Methods for identifying modulators of p53, comprising screening for agents that modulate the activity of SCD are provided.

摘要翻译： 人类SCD基因被鉴定为p53途径的调节剂，因此是与缺陷型p53功能相关的病症的治疗靶点。 提供了鉴定p53调节剂的方法，包括筛选调节SCD活性的药剂。