公开(公告)号：US20150135119A1

公开(公告)日：2015-05-14

申请号：US14598878

申请日：2015-01-16

Applicant: EMD MILLIPORE CORPORATION

Inventor： Amedeo Joseph Cappione, III ,  Ray Lefebvre ,  Neil Kilcoin ,  David Andrew King ,  George J. Dixon

IPC: G06F19/26 ,  G06F3/0481

CPC classification number: G06F19/26 ,  G01N35/00722 ,  G01N2015/1402 ,  G01N2015/1477 ,  G01N2035/0091 ,  G06F3/04817

Abstract: A graphical user interface on a computer provides for the analysis of location specific data and the presentation of analysis results for visual comparison by a user. Results of the analysis are visually presented as icons subdivided into regions and arranged in such a way that the user is able to associate each icon with a data location. A visual presentation of results in the icons and regions allows a user to visually compare the analysis results in two or more data sets according to location. The graphical user interface further provides for the definition and adjustment of an analysis through the interaction of a user with a graphical representation of the analysis. In some cases, the visual presentation of results tracks the analysis adjustments so the user can visually observe the effects that the adjustments have on the results. A method of interacting with the interface to define an analysis and represent results and a method of presenting two or more data sets using the interface are described. The interface can be used to analyze and visually compare the results of location specific data from a number of sources and is illustrated in a flow cytometry application.

Abstract translation: 计算机上的图形用户界面提供对位置特定数据的分析和分析结果的呈现，以供用户视觉比较。 分析结果以视觉方式呈现为按区域划分的图标，并以这样一种方式排列，使得用户能够将每个图标与数据位置相关联。 在图标和区域中的视觉呈现结果允许用户根据位置在两个或多个数据集中视觉比较分析结果。 图形用户界面还通过用户与分析的图形表示的交互来进一步提供对分析的定义和调整。 在某些情况下，结果的视觉呈现跟踪分析调整，以便用户可以直观地观察调整对结果的影响。 描述了与接口交互以定义分析并表示结果的方法以及使用该接口呈现两个或多个数据集的方法。 界面可用于分析和视觉比较来自多个来源的位置特定数据的结果，并在流式细胞术应用中进行了说明。

公开(公告)号：US20150133643A1

公开(公告)日：2015-05-14

申请号：US14400392

申请日：2013-06-06

Applicant: EMD Millipore Corporation

Inventor： Kwok-Shun Cheng ,  Nripen Singh

IPC: C07K1/34 ,  C07K16/00

CPC classification number: C07K1/34 ,  C07K16/00 ,  C12M47/02

Abstract: Provided is a primary clarification depth filtration process of cell-culture feeds, including chemically treated flocculated feeds, containing target biomolecules of interest such as mAbs, mammalian cell cultures, or bacterial cell cultures, utilizing a primary clarification depth filtration device containing a media with significantly lower flushing requirements, resulting in lower levels of organic extractables released after media flushing, and increased throughput for the pre-treated feed streams, without the use of a primary clarification centrifugation step or primary clarification tangential flow microfiltration step. The primary clarification depth filtration device used in the primary clarification of fluid cell culture feeds, including chemically treated flocculated feeds containing flocculated cellular debris and/or colloidal particulates having a particle size distribution of about 0.5 μm to 200 um, contains a porous depth filter media having porous layers of varying pore ratings, and achieves the desired level, of total organic extractables (1-3 ppm) measured in the feed filtered through the media with, significantly lower flushing requirements. Kits and methods of using and making the same are also provided.

Abstract translation: 提供了细胞培养饲料的初步澄清深度过滤方法，其包括化学处理的絮凝饲料，其包含感兴趣的目标生物分子，例如mAb，哺乳动物细胞培养物或细菌细胞培养物，利用含有显着的培养基的初级澄清深度过滤装置 更低的冲洗要求，导致在介质冲洗后释放的有机提取物水平较低，并且增加了预处理的进料流的通过量，而不使用初级澄清离心步骤或初级澄清切向流微量过滤步骤。 用于初步澄清流体细胞培养物料的初级澄清深度过滤装置，包括含有絮凝细胞碎片的化学处理的絮凝饲料和/或具有约0.5μm至200μm的粒度分布的胶体颗粒，其包含多孔深度过滤介质 具有不同孔径等级的多孔层，并达到在通过介质过滤的进料中测量的总有机可提取物（1-3ppm）的期望水平，显着降低了冲洗要求。 还提供了使用和制造相同的套件和方法。

公开(公告)号：US20150061865A1

公开(公告)日：2015-03-05

申请号：US14537185

申请日：2014-11-10

Applicant: EMD Millipore Corporation

Inventor： George Adams ,  John Dana Hubbard ,  Aaron Burke ,  Anthony DiLeo

IPC: G01N35/00

CPC classification number: G01N35/00732 ,  B01L3/505 ,  B01L3/545 ,  B01L2300/022 ,  C12M33/00 ,  C12M33/06 ,  C12M37/00 ,  G01N1/18 ,  G01N2001/1031 ,  G01N2001/205 ,  G01N2035/00782 ,  G01N2035/00801

Abstract: The present invention uses a wireless memory/communication device at least on the one or more sample storage devices, preferably on both the one or more sample storage devices and the sampling holder, optionally the port on the equipment as well. Data such as that relating to the vessel, the location of the port on the vessel, the device, its manufacture date or lot number, the date of the installation, sterilization and/or taking of a sample along with the person who installed the device and/or took the sample can he read and preferably added to the wireless device when a read/write type of device as these events occur through a scanner/reader/writer device (fixed or hand held). The sample storage device in the laboratory can also then be read and recorded to track the sample storage device's life.

Abstract translation: 本发明至少在至少一个或多个样本存储设备上使用无线存储器/通信设备，优选地在一个或多个采样存储设备和采样保持器两者上，可选地，设备上的端口也使用无线存储器/通信设备。 与船舶有关的数据，船上港口的位置，设备，其制造日期或批号，安装日期，灭菌和/或采样样品以及安装设备的人员 并且/或者当通过扫描器/读取器/写入器设备（固定或手持）发生这些事件的读/写类型的设备时，他可以读取样本并且优选地添加到无线设备。 实验室中的样品存储设备也可以被读取和记录，以跟踪样品存储设备的使用寿命。

公开(公告)号：US08919365B2

公开(公告)日：2014-12-30

申请号：US13956428

申请日：2013-08-01

Applicant: EMD Millipore Corporation

Inventor： Brian Hillier ,  Martin Szyk ,  Aaron Burke ,  Joseph Almasian

IPC: F16K15/00 ,  F16K31/00 ,  F16K31/50 ,  F16K1/12 ,  F16K11/22 ,  F16K31/44 ,  F16K31/60 ,  G01N15/08

CPC classification number: F16K31/50 ,  F16K1/12 ,  F16K11/22 ,  F16K31/445 ,  F16K31/60 ,  G01N15/0826 ,  Y10T137/0318 ,  Y10T137/7932 ,  Y10T137/794 ,  Y10T137/85938 ,  Y10T137/87885

Abstract: Fluid transfer apparatus including a body having a bore formed through at least a portion of its interior. Contained within the bore is a movable plunger that moves without changing the axial dimensions of the body. A first end of the body contains a face designed to be attached to an upstream component. A second end of the body is connected to a downstream component such as a filter, pipeline, etc. A first end of the plunger, when it is in the closed position, is in alignment with the face of the body, which combined form a steamable surface and a sterile barrier against the environment to the remainder of the interior of the body, the plunger and downstream components. An outer annular collar is rotatable relative to the body and causes the plunger to move axially within the bore from an open to a closed position.

Abstract translation: 流体输送装置包括具有通过其内部的至少一部分形成的孔的主体。 包含在孔内的是可移动的柱塞，其移动而不改变主体的轴向尺寸。 身体的第一端包含设计成连接到上游部件的面。 主体的第二端连接到诸如过滤器，管道等的下游部件。当处于关闭位置时，柱塞的第一端与主体的表面对准，其结合形成一个 可蒸发的表面和抵抗环境的无菌屏障到身体内部的其余部分，柱塞和下游部件。 外环形套环可相对于主体旋转并使柱塞在孔内轴向移动，从开放到闭合位置。

公开(公告)号：US08904641B2

公开(公告)日：2014-12-09

申请号：US13754928

申请日：2013-01-31

Applicant: EMD Millipore Corporation

Inventor： Sal Giglia ,  Kevin Rautio ,  Mark Blanchard

IPC: B23P15/16 ,  B01D69/02 ,  B01D65/10 ,  G01N15/08

CPC classification number: B01D69/02 ,  B01D65/10 ,  B01D2325/20 ,  G01N15/0826 ,  Y10T29/496 ,  Y10T29/49604 ,  Y10T29/4978

Abstract: Method of reducing performance variability of membrane scaling devices. Scaling device performance uncertainty is reduced, thereby reducing the scaling safety factor, by specifying a narrow range or subset of the set of all qualified manufactured membranes for installation into scaling devices. In certain embodiments, the scalability factor is reduced by determining where within the performance distribution a particular membrane lies, and adjusting the scaling factor accordingly.

Abstract translation: 降低膜缩放装置的性能变异性的方法。 缩小设备性能不确定度，从而通过指定所有合格制造膜组的窄范围或子集来安装到缩放设备中，从而降低了比例安全系数。 在某些实施例中，通过确定特定膜所在的性能分布内的哪里以及相应地调整缩放因子来减小可伸缩性因子。

公开(公告)号：US20140323697A1

公开(公告)日：2014-10-30

申请号：US14325014

申请日：2014-07-07

Applicant: EMD Millipore Corporation

Inventor： Nanying Bian ,  Senthil Ramaswamy ,  Neil Soice ,  Chen Wang ,  Yuan Wong

IPC: C07K1/22 ,  B01J20/286 ,  C07K16/00

CPC classification number: C07K16/00 ,  B01J20/103 ,  B01J20/28004 ,  B01J20/28014 ,  B01J20/28016 ,  B01J20/28026 ,  B01J20/28054 ,  B01J20/28085 ,  B01J20/281 ,  B01J20/283 ,  B01J20/286 ,  C03C11/00 ,  C03C12/00 ,  C03C17/28 ,  C07K1/22 ,  G01N33/538 ,  Y10T428/2982

Abstract: The invention relates generally to solid supports for chromatography. In specific embodiments the invention provides for solid supports suitable for affinity chromatography along with methods, systems and kits which use the same.

Abstract translation: 本发明一般涉及用于色谱的固体支持物。 在具体实施方案中，本发明提供了适用于亲和层析的固体支持物，以及使用其的方法，系统和试剂盒。

公开(公告)号：US08844388B2

公开(公告)日：2014-09-30

申请号：US14080466

申请日：2013-11-14

Applicant: EMD Millipore Corporation

Inventor： Aaron Burke

IPC: G01D21/00 ,  A61L2/26 ,  G01L19/06 ,  B01D63/00 ,  C12M1/12

CPC classification number: C12M37/02 ,  A61L2/26 ,  B01D63/00 ,  C12M23/14 ,  C12M29/00 ,  C12M41/00 ,  G01L19/0645

Abstract: A system and method for interfacing non-sterile sensors to a sterile flow stream is disclosed. Typically, sensors cannot be sterilized in the same manner as other components of the flow stream. This results in complex processes to incorporate a sterilized sensor into a sterilized flow stream. By introducing a separation membrane, the desired sensor can be interfaced to the sterile flow stream. By doing so, the sensor need not be sterile, only sufficiently clean. The membrane separates the sterile environment within the flow stream from the sensor, while still permitting the sensor to function.

公开(公告)号：US20140221629A1

公开(公告)日：2014-08-07

申请号：US14189196

申请日：2014-02-25

Applicant: EMD Millipore Corporation

Inventor： Gabriel Tkacik ,  Philip Goddard ,  Willem Kools ,  Nitin Satav

IPC: C07K1/34

CPC classification number: B01D69/12 ,  B01D61/145 ,  B01D67/0013 ,  B01D67/0016 ,  B01D67/0018 ,  B01D71/34 ,  B01D71/68 ,  B01D2323/12 ,  B01D2323/42 ,  B01D2325/022 ,  C07K1/34

Abstract: The present invention is an integral multilayered composite membrane having at least one ultrafiltration layer made by cocasting or sequentially casting a plurality of polymer solutions onto a support to form a multilayered liquid sheet and immersing the sheet into a liquid coagulation bath to effect phase separation and form a multilayered composite membrane having at least one ultrafiltration layer.

公开(公告)号：US20140171594A1

公开(公告)日：2014-06-19

申请号：US14183791

申请日：2014-02-19

Applicant: EMD Millipore Corporation

Inventor： Jad Jaber ,  Wilson Moya ,  James Hamzik ,  Arezki Boudif ,  Yu Zhang ,  Neil Soice ,  John Charkoudian ,  Nripen Singh

IPC: C08F126/02 ,  C07K16/00

CPC classification number: B01J41/14 ,  B01J41/13 ,  C07K1/30 ,  C07K1/32 ,  C07K16/00 ,  C07K2317/10 ,  C08F8/00 ,  C08F8/02 ,  C08F126/02 ,  C08F26/02

Abstract: The present invention provides novel and improved stimulus responsive polymers and methods of using the same for the purification of biomolecules.

Abstract translation: 本发明提供了新的和改进的刺激响应聚合物以及使用其进行生物分子纯化的方法。

公开(公告)号：US20140073769A1

公开(公告)日：2014-03-13

申请号：US14083590

申请日：2013-11-19

Applicant: EMD Millipore Corporation

Inventor： Kwok-Shun Cheng ,  Senthilkumar Ramaswamy ,  Chen Wang ,  Nanying Bian ,  Brian Gagnon ,  Joaquin A. Umana ,  Dennis Aquino ,  Neil Soice ,  Lyddiatt Andrew

IPC: C07K16/00

CPC classification number: C07K16/00 ,  B01J20/262 ,  B01J20/265 ,  B01J20/267 ,  B01J20/28019 ,  B01J20/28092 ,  B01J20/285 ,  B01J20/286 ,  B01J20/3212 ,  B01J20/3244 ,  B01J20/3274 ,  B01J20/328 ,  B01J20/3285 ,  B01J20/3293 ,  B01J39/26 ,  Y10T428/2982

Abstract: The present invention relates to an asymmetric chromatography media suitable for separations applications, particularly as packed bed, fluidized bed or magnetized bed chromatography media. In certain embodiments, the asymmetric chromatography media comprises asymmetric particles, preferably beads, having at least two distinct, controlled pore size distributions. Preferably one of the distinct pore size distributions is in an internal region of the particle, and the other is in an external region or coating on the particle. These distinct pore size distributions can be modified with uniform or alternatively unique functional groups or mixtures of functional groups. The present invention allows for the control over pore size distribution within an asymmetric porous particle by providing a distinct internal region, preferably in the form of a bead, and a distinct external region, preferably in the form of a coating on the bead.

Abstract translation: 本发明涉及适用于分离应用的非对称色谱介质，特别是作为填充床，流化床或磁化床层析介质。 在某些实施方案中，不对称色谱介质包含具有至少两个不同的，受控的孔径分布的不对称颗粒，优选珠粒。 优选地，不同孔径分布中的一个在颗粒的内部区域中，另一个位于颗粒的外部区域或涂层中。 这些不同的孔径分布可以用均匀的或替代地独特的官能团或官能团的混合物进行改性。 本发明允许通过提供不同的内部区域，优选以珠粒的形式和不同的外部区域，优选以珠粒上的涂层的形式来控制不对称多孔颗粒内的孔径分布。