公开(公告)号：US07253003B2

公开(公告)日：2007-08-07

申请号：US10045937

申请日：2001-10-19

Applicant: David J. Beebe ,  Jaisree Moorthy

Inventor： David J. Beebe ,  Jaisree Moorthy

IPC: G01N33/48

CPC classification number: B01L3/5027 ,  B01L2200/143 ,  B01L2300/0627 ,  G01N21/80 ,  Y10T436/2575

Abstract: A method and apparatus is provided for monitoring the environment within a microfluidic device. The microfluidic device includes a body defining a channel for accommodating flow of fluid therethrough. A monitor structure is disposed in the channel of the body in the flow of fluid. The monitor structure changes color and/or dimension in response to various parameters of the fluid having predetermined values.

Abstract translation: 提供了一种用于监测微流体装置内的环境的方法和装置。 微流体装置包括限定用于容纳流体流过其中的通道的主体。 监测器结构设置在流体流动中的身体的通道中。 响应于具有预定值的流体的各种参数，监视器结构改变颜色和/或尺寸。

公开(公告)号：US07111635B2

公开(公告)日：2006-09-26

申请号：US10269476

申请日：2002-10-11

Applicant: David J. Beebe ,  Jeffrey S. Moore ,  Jaisree Moorthy

Inventor： David J. Beebe ,  Jeffrey S. Moore ,  Jaisree Moorthy

IPC: F17D1/18 ,  F15B21/00

CPC classification number: B81C1/00119 ,  B01L3/502707 ,  B01L3/502753 ,  B01L2200/0668 ,  B01L2200/12 ,  B01L2300/0681 ,  B81B2201/058 ,  B81B2203/0361 ,  Y10T137/0396 ,  Y10T137/2076 ,  Y10T137/2191 ,  Y10T137/2196 ,  Y10T137/2224

Abstract: A method is provided for fabricating a constriction region in a channel of a microfluidic device. The method includes the steps of introducing a pre-polymer mixture including a monomer, cross-linking agent and photoinitiator into the channel. The pre-polymer mixture is polymerized at a localized area of the channel so as to shrink and solidify the liquid mixture. The solidified and shrunken liquid mixture provides the constriction region in the channel.

公开(公告)号：US06821898B2

公开(公告)日：2004-11-23

申请号：US09952243

申请日：2001-09-14

Applicant: David J. Beebe ,  Glennys A. Mensing

Inventor： David J. Beebe ,  Glennys A. Mensing

IPC: H01L21301

CPC classification number: B81C1/00119 ,  B81B2201/058 ,  B81B2203/0353 ,  B81C2201/019

Abstract: A method is provided for fabricating a multi-layer microfluidic device on a base. A first layer is positioned on the base in a spaced relationship thereto so as to define a construction cavity therebetween. The first layer has a passageway therethrough which communicates with the construction cavity. A mask is positioned between the construction cavity and an ultraviolet source. The mask corresponds to a channel to be formed in the construction cavity. The construction cavity is filled with material and a portion of the material is polymerized within the construction cavity so as to solidify the same. The solidified material defines the channel. Thereafter, the material is flushed from the channel in the construction cavity.

Abstract translation: 提供了一种在基底上制造多层微流体装置的方法。 第一层以与其间隔开的关系定位在基座上，以便在它们之间限定结构空腔。 第一层具有通过其与构造空腔连通的通道。 掩模位于结构腔和紫外线源之间。 掩模对应于要在结构空腔中形成的通道。 结构空腔填充材料，并且部分材料在结构空腔内聚合以使其固化。 固化材料定义通道。 此后，材料从结构腔中的通道冲洗。

公开(公告)号：US06695765B1

公开(公告)日：2004-02-24

申请号：US09570483

申请日：2000-05-12

Applicant: David J. Beebe ,  Ian K. Glasgow ,  Matthew B. Wheeler ,  Henry Zeringue

Inventor： David J. Beebe ,  Ian K. Glasgow ,  Matthew B. Wheeler ,  Henry Zeringue

IPC: A61B1743

CPC classification number: B01L3/502761 ,  A61B17/435 ,  A61D19/04 ,  B01L3/502707 ,  B01L2200/143 ,  B01L2400/0457 ,  B01L2400/0487 ,  B01L2400/0688 ,  B01L2400/086 ,  C12M21/06 ,  C12M23/16 ,  C12M29/10 ,  C12M41/00

Abstract: Microfluidic embryo scaled channels for handling and positioning embryos provide the opportunity to evaluate and treat embryos in improved manners Fluid flow is used to move and position embryos within microfluidic channels and channel geometries may be used to place embryos at specific locations. Surface properties and compliance (deformation) properties of embryos are evaluated as a predictor of viability. The microfluidic channels provide the opportunity for fine controls of pressure to conduct various evaluations at forces slightly below which damage to embryos is known to occur.

Abstract translation: 用于处理和定位胚胎的微流体胚胎标准化通道提供了以改进方式评估和治疗胚胎的机会。流体流动用于移动和定位微流体通道内的胚胎，并且通道几何形状可用于将胚胎放置在特定位置。 胚胎的表面性质和顺应性（变形）性质被评估为生存力的预测因子。 微流体通道提供精确控制压力的机会，在稍低于胚胎已知发生损伤的力下进行各种评估。

公开(公告)号：US06488872B1

公开(公告)日：2002-12-03

申请号：US09625204

申请日：2000-07-21

Applicant: David J. Beebe ,  Jeffrey S. Moore

Inventor： David J. Beebe ,  Jeffrey S. Moore

IPC: E04B116

CPC classification number: G03F7/00 ,  B01J19/0093 ,  B01J2219/00828 ,  B01J2219/00831 ,  B01J2219/00833 ,  B01J2219/00891 ,  B01J2219/00925 ,  B01J2219/00936 ,  B01J2219/00952 ,  B01J2219/00966 ,  B01L3/502707 ,  B01L3/502738 ,  B01L3/502746 ,  B01L3/502776 ,  B01L2200/12 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/0883 ,  B01L2300/16 ,  B01L2300/161 ,  B01L2400/0661 ,  G03F7/0035

Abstract: Microfabricated devices and methods of manufacturing the devices are disclosed. The devices are manufactured from a substrate having microscale fluid channels, and polymerizing a polymerizable mixture in the channels to form stimuli-responsive operating components of the device. The operating components can be functional or structural components. The method of manufacture obviates the traditional assembly of microscale components to form a device because the microscale components are formed in situ on or within the device.

Abstract translation: 公开了微型装置和制造装置的方法。 这些装置由具有微量流体通道的基底制造，并且在通道中聚合可聚合混合物以形成装置的刺激响应性操作部件。 操作部件可以是功能或结构部件。 制造方法消除了微量元件的传统组装以形成器件，因为微量元件在器件上或器件内原位形成。

公开(公告)号：US06193647B1

公开(公告)日：2001-02-27

申请号：US09289137

申请日：1999-04-08

Applicant: David J. Beebe ,  Ian K. Glasgow ,  Matthew B. Wheeler

Inventor： David J. Beebe ,  Ian K. Glasgow ,  Matthew B. Wheeler

IPC: A61B1743

CPC classification number: B01L3/502761 ,  A61B17/435 ,  A61D19/04 ,  B01L3/502707 ,  B01L2200/143 ,  B01L2400/0457 ,  B01L2400/0487 ,  B01L2400/0688 ,  B01L2400/086 ,  C12M21/06 ,  C12M23/16 ,  C12M29/10 ,  C12M41/00

Abstract: A microfluidic embryo handling device and method in which biological rotating of embryos is simulated. Fluid flow is used to move and position embryos without assistance of electrical stimulus or other means which may produce undesired heating of biological medium used as the fluid for transporting and position. Continuous or pulsed flow is maintained around an embryo or embryos in the device. The device provides an excellent simulation of biological conditions and may be used for culturing, sorting, testing, evaluating, fertilizing and other similar typical handling operations. An embryo may be parked at a desired location to carry out such a typical operation, while the microfluidic handling device maintains fluid flow around the embryo.

Abstract translation: 一种微流体胚胎处理装置和方法，其中模拟胚胎的生物旋转。 流体流动用于移动和定位胚胎，而无需电刺激或其他可能产生用作运输和定位的流体的生物介质的不期望的加热的手段。 在装置中的胚胎或胚胎周围保持连续或脉冲流动。 该设备提供了良好的生物条件模拟，可用于培养，分选，测试，评估，施肥和其他类似的典型处理操作。 胚胎可以停放在期望的位置以执行这种典型的操作，而微流体处理装置保持围绕胚胎的流体流动。

公开(公告)号：US09157550B2

公开(公告)日：2015-10-13

申请号：US12652735

申请日：2010-01-05

Applicant: Matthew B. Wheeler ,  Dongshin Kim ,  David J. Beebe

Inventor： Matthew B. Wheeler ,  Dongshin Kim ,  David J. Beebe

IPC: C12M1/34 ,  F16K99/00 ,  C12M3/06 ,  C12M3/00 ,  A61B17/425 ,  B01L3/00

CPC classification number: F16K99/0001 ,  A61B17/425 ,  B01L3/5085 ,  B01L2300/0829 ,  C12M21/06 ,  C12M23/16 ,  F16K99/0017 ,  F16K99/0028 ,  Y10T137/0318 ,  Y10T137/87249

Abstract: Microfluidic systems and methods. A microfluidic device for in vitro fertilization comprises a substrate and a plurality of microchannels disposed in the substrate, including an inlet of at least two of the plurality of microchannels arranged on the substrate to align with a fluid-handling device. Another microfluidic system for assaying a plurality of cells comprises a substrate and a plurality of microfluidic channels comprising a source channel, a sink channel, and a cell chamber. An insert for a microfluidic system comprises a substrate configured to be inserted into a dish and a plurality of microscale wells disposed in the substrate. A microfluidic channel comprises a substrate and at least one microchannel having an open inlet, an open outlet, a channel, and an opening in the substrate disposed over a portion of the channel. A device for providing an amount of fluid for a fluidic system comprises a main reservoir, an aspiration well, tubing coupling the reservoirs, and a seal closing the main reservoir. Air tubing having a hydrophobic end extends into the at least one aspiration well.

Abstract translation: 微流体系统和方法。 用于体外受精的微流体装置包括衬底和设置在衬底中的多个微通道，包括布置在衬底上的多个微通道中的至少两个的入口，以与流体处理装置对准。 用于测定多个细胞的另一个微流体系统包括底物和多个包含源通道，宿通道和细胞室的微流体通道。 用于微流体系统的插入件包括构造成插入盘中的衬底和设置在衬底中的多个微孔。 微流体通道包括基底和至少一个具有开放入口，开口的出口，通道和布置在通道的一部分上的基底中的开口的微通道。 用于为流体系统提供一定量的流体的装置包括主储存器，抽吸井，联接储存器的管道和封闭主储存器的密封件。 具有疏水端的空气管延伸到至少一个抽吸井中。

公开(公告)号：US08728411B2

公开(公告)日：2014-05-20

申请号：US13604192

申请日：2012-09-05

Applicant: David J. Beebe ,  Scott M. Berry ,  David J. Guckenberger ,  Ben P. Casavant

Inventor： David J. Beebe ,  Scott M. Berry ,  David J. Guckenberger ,  Ben P. Casavant

IPC: B01D21/00 ,  B01L3/00 ,  B01D59/48 ,  B01D17/06 ,  B03C5/02

CPC classification number: G01N1/34 ,  B01L3/502761 ,  B01L3/502792 ,  B01L2200/0668 ,  B01L2200/0673 ,  B01L2300/087 ,  B01L2400/043 ,  G01N1/4077 ,  G01N35/0098

Abstract: A device and a method are provided for isolating a fraction in a biological sample. The fraction is bound to solid phase substrate to define a fraction-bound solid phase substrate. The device includes an input zone for receiving the biological sample therein and a second zone for receiving an isolation fluid therein. A force is provided that is generally perpendicular to gravity. The force is movable between a first position adjacent the input zone and a second position adjacent the isolation zone. The force captures the fraction-bound solid phase substrate and the fraction-bound solid phase substrate moves from the input zone to the isolation zone in response to the force moving from the first position to the second position.

Abstract translation: 提供了用于分离生物样品中的级分的装置和方法。 该馏分与固相底物结合以限定分馏结合的固相底物。 该装置包括用于在其中接收生物样品的输入区和用于在其中接收隔离流体的第二区。 提供通常垂直于重力的力。 该力可在邻近输入区的第一位置与邻近隔离区的第二位置之间移动。 该力捕获分馏结合的固相底物，并且部分结合的固相基板响应于从第一位置移动到第二位置的力而从输入区移动到隔离区。

公开(公告)号：US08652852B2

公开(公告)日：2014-02-18

申请号：US13272433

申请日：2011-10-13

Applicant: David J. Beebe ,  Jay W. Warrick ,  Michael W. Toepke ,  Ivar Meyvantsson ,  Glenn M. Walker

Inventor： David J. Beebe ,  Jay W. Warrick ,  Michael W. Toepke ,  Ivar Meyvantsson ,  Glenn M. Walker

IPC: G01N15/06

CPC classification number: F04B19/006 ,  B01L3/50273 ,  B01L2300/0816 ,  B01L2400/0406 ,  B01L2400/0457 ,  Y10T137/0357 ,  Y10T137/0363 ,  Y10T137/0379 ,  Y10T137/0396 ,  Y10T436/11 ,  Y10T436/117497 ,  Y10T436/118339 ,  Y10T436/2575

Abstract: A method is provided for pumping fluid through a channel of a microfluidic device. The channel has an input port and an output port. The channel is filled with fluid and a pressure gradient is generated between the fluid at the input port and the fluid at the output port. As a result, fluid flows through the channel towards the output port.

Abstract translation: 提供了一种用于泵送流体通过微流体装置的通道的方法。 通道有一个输入端口和一个输出端口。 通道填充有流体，并且在输入端口处的流体和输出端口处的流体之间产生压力梯度。 结果，流体流过通道朝向输出端口。

公开(公告)号：US08603416B2

公开(公告)日：2013-12-10

申请号：US12713950

申请日：2010-02-26

Applicant: David J. Beebe ,  Scott M. Barry

Inventor： David J. Beebe ,  Scott M. Barry

IPC: B03C1/02

CPC classification number: C07K1/145 ,  C07K1/22

Abstract: A device and method are provided for facilitating extraction of a fraction from a biological sample. The biological sample includes non-desired material and a fraction-bound solid phase substrate. The device includes an input zone for receiving the biological sample therein and a phase-gate zone for receiving an isolation buffer therein. An output zone receives a reagent therein. A force is movable between a first position adjacent the input zone and a second position adjacent the output zone. The force urges the fraction-bound solid phase substrate from the input zone, through the phase-gate zone and into the output zone.

Abstract translation: 提供了一种用于促进从生物样品中提取级分的装置和方法。 生物样品包括非期望的材料和分数结合的固相底物。 该装置包括用于在其中接收生物样本的输入区和用于在其中接收隔离缓冲器的相门区。 输出区在其中接收试剂。 力可在邻近输入区的第一位置与邻近输出区的第二位置之间移动。 力从输入区域通过相位闸区促进分数结合的固相基板进入输出区域。