公开(公告)号：US10703774B2

公开(公告)日：2020-07-07

申请号：US15348699

申请日：2016-11-10

Applicant: GE Healthcare BioProcess R&D AB

Inventor： Annika Kristina Forss ,  Gustav Jose Rodrigo ,  Tomas Bjorkman ,  Jesper Ulf Hansson ,  Mats Ander

IPC: C07K1/22 ,  B01D15/00 ,  C07K16/12 ,  C07K17/10 ,  B01J20/286 ,  C07K14/31 ,  C07K16/06 ,  B01J20/32

Abstract: The invention relates to a method of isolating an immunoglobulin, comprising the steps of:a) providing a separation matrix comprising multimers of immunoglobulin-binding alkali-stabilized Protein A domains covalently coupled to a porous support,b) contacting a liquid sample comprising an immunoglobulin with the separation matrix,c) washing said separation matrix with a washing liquid,d) eluting the immunoglobulin from the separation matrix with an elution liquid, ande) cleaning the separation matrix with a cleaning liquid,wherein the alkali-stabilized Protein A domains comprise mutants of a parental Fc-binding domain of Staphylococcus Protein A (SpA), as defined by, or having at least 80% such as at least 90%, 95% or 98% identity to, SEQ ID NO 51 or SEQ ID NO 52, wherein the amino acid residues at positions 13 and 44 of SEQ ID NO 51 or 52 are asparagines and wherein at least the asparagine residue at position 3 of SEQ ID NO 51 or 52 has been mutated to an amino acid selected from the group consisting of glutamic acid, lysine, tyrosine, threonine, phenylalanine, leucine, isoleucine, tryptophan, methionine, valine, alanine, histidine and arginine.

公开(公告)号：US10662261B2

公开(公告)日：2020-05-26

申请号：US15937428

申请日：2018-03-27

Applicant: GE HEALTHCARE BIOPROCESS R&D AB

Inventor： Mattias Algotsson ,  Lars C. Andersson ,  Eggert Brekkan ,  Lee Hedemyr ,  Jean-Luc Maloisel ,  Ronnie Palmgren

IPC: C08B37/00 ,  B01J20/289 ,  B01J20/32 ,  C07C237/10 ,  C07C323/60 ,  B01J20/281

Abstract: The present invention relates to dimeric pentadentate chelators with exceptionally strong binding of metal ions, for detection, immobilization and purification of biomolecules. Dimeric chelators offer a cooperativity of binding of two adjacent immobilized metal ions simultaneously to a histidine-tagged biomolecule, which gives advantageous properties regarding strength of binding compared to a corresponding monomer chelator. In addition, a dimer increases the selectivity (ease of separation) against non-tagged biomolecules with low metal-ion affinity.

公开(公告)号：US20190263856A1

公开(公告)日：2019-08-29

申请号：US16348534

申请日：2017-11-14

Applicant: GE Healthcare BioProcess R&D AB

Inventor： Johan Ohman ,  Mats Ander ,  Therese Graner ,  Anneli Jorsback ,  Jinyu Zou

IPC: C07K1/22 ,  B01J20/24 ,  B01J20/285 ,  B01J20/286 ,  B01J20/32 ,  B01D15/38

Abstract: The present invention relates to the field of chromatography and more specifically to producing protein affinity chromatography resins comprising affinity ligands based on a N-terminal fragment of a split intein, such as DnaE from Nostoc punctiforme, as well as methods for using the same. The N-terminal fragments are produced in inclusion bodies in bacterial cells.

公开(公告)号：US20190184358A1

公开(公告)日：2019-06-20

申请号：US16328129

申请日：2017-09-04

Applicant: GE Healthcare BioProcess R&D AB

Inventor： Jimmy Hedin Dahlstrom ,  Jonas Bengtsson ,  Per Erik Emilsson ,  Maranda Fordham ,  Eva Holmgren ,  Ronnie Palmgren ,  Fernando Polanco

IPC: B01J2/06 ,  B01F3/08

CPC classification number: B01J2/06 ,  B01F3/0811 ,  B01F5/0696 ,  B01F2003/0834 ,  B01F2003/0842

Abstract: A system and method for producing fine droplets of polysaccharide from a premixed water-in-oil emulsion uses a packed bed (5) comprising hydrophilic beads (7).

公开(公告)号：US20190023735A1

公开(公告)日：2019-01-24

申请号：US16115756

申请日：2018-08-29

Applicant: GE Healthcare BioProcess R&D AB

Inventor： Jean-Luc Maloisel ,  Gustav Rodrigo ,  Bjorn Noren ,  Virendra Kumbhar

IPC: C07K1/16 ,  B01J20/32 ,  C07K1/36 ,  B01J20/28 ,  B01J20/26 ,  B01D15/38 ,  G01N33/544 ,  B01J20/289 ,  B01J41/20 ,  B01D15/36 ,  B01D15/32

Abstract: The invention discloses a separation matrix which comprises a plurality of separation ligands, defined by the formula R1-L1-N(R3)-L2-R, immobilized on a support, wherein R1 is a five- or six-membered, substituted or non-substituted ring structure or a hydroxyethyl or hydroxypropyl group; L1 is either a methylene group or a covalent bond; R2 is a five-or six-membered, substituted or non-substituted ring structure; L2 is either a methylene group or a covalent bond; R3 is a methyl group; and wherein if R1 is a hydroxyethyl group and L1 is a covalent bond, R2 is a substituted aromatic ring structure or a substituted or non-substituted aliphatic ring structure.

公开(公告)号：US10124328B2

公开(公告)日：2018-11-13

申请号：US15407240

申请日：2017-01-16

Applicant: GE Healthcare BioProcess R&D AB

Inventor： Jesper Hansson ,  Gustav Rodrigo ,  Tobias E Soderman

IPC: B01J39/26 ,  B01D15/18 ,  B01D15/36 ,  B01J20/286 ,  B01J20/32 ,  B01D15/38 ,  B01J20/26 ,  B01J20/28 ,  B01J20/289 ,  B01J20/30 ,  C07H1/08 ,  C07K1/22 ,  B01D15/34 ,  B01J20/24 ,  G01N30/88

Abstract: The invention discloses a method of separating a biomolecule from at least one other component in a liquid, comprising a step of contacting said liquid with a separation matrix comprising a solid support and polymer chains bound to said solid support. The polymer chains comprise units derived from a first monomer of structure CH2═CH-L-X, where L is a covalent bond or an alkyl ether or hydroxysubstituted alkyl ether chain comprising 2-6 carbon atoms, and X is a sulfonate or phosphonate group.

公开(公告)号：US20180291184A1

公开(公告)日：2018-10-11

申请号：US15578074

申请日：2016-05-27

Applicant: GE HEALTHCARE BIOPROCESS R&D AB

Inventor： Per Erik Emilsson ,  Susanna Klara Margareta Lindberg ,  Jonny Wernersson ,  Jonas Afsson ,  Adam Hurynowicz

IPC: C08L5/02 ,  C08B37/02 ,  C12N5/00 ,  C07K1/22 ,  B01J13/14

Abstract: The invention discloses a method of manufacturing polysaccharide beads, comprising the steps of: i) providing a water phase comprising an aqueous solution of a polysaccharide; ii) providing an oil phase comprising at least one water-immiscible organic solvent and at least one oil-soluble emulsifier; iii) emulsifying the water phase in the oil phase to form a water-in-oil (w/o) emulsion; and iv) inducing solidification of the water phase in the w/o emulsion, wherein the organic solvent is an aliphatic or alicyclic ketone or ether.

公开(公告)号：US20180244729A1

公开(公告)日：2018-08-30

申请号：US15900419

申请日：2018-02-20

Applicant: GE Healthcare BioProcess R&D AB

Inventor： Gustav Rodrigo ,  Mats Ander ,  Tomas BJORKMAN ,  Goran Bauren

IPC: C07K14/31 ,  B01D15/38 ,  C07K14/745 ,  C07K1/22 ,  C07K16/00 ,  B01J20/32 ,  B01J20/286

CPC classification number: C07K14/31 ,  B01D15/3809 ,  B01J20/286 ,  B01J20/3204 ,  B01J20/3212 ,  B01J20/3219 ,  B01J20/3274 ,  C07K1/22 ,  C07K14/745 ,  C07K16/00

Abstract: The invention discloses a polypeptide with improved alkaline stability, which polypeptide comprises a mutant of a B or C domain of Staphylococcus Protein A (SpA), as specified by SEQ ID NO 1 or SEQ ID NO 2, or of Protein Z, as specified by SEQ ID NO 3, wherein at least the glutamine residue at position 9 has been mutated to an amino acid other than asparagine. The invention also discloses multimers of said polypeptide, as well as separation matrices comprising the multimers or polypeptides.

公开(公告)号：US20180044374A1

公开(公告)日：2018-02-15

申请号：US15798784

申请日：2017-10-31

Applicant: GE HEALTHCARE BIOPROCESS R&D AB

Inventor： Annika Forss ,  Mats Ander ,  Tomas Bjorkman ,  Hans Blom ,  Jesper Hansson ,  Gustav Rodrigo

IPC: C07K1/16 ,  B01D15/18 ,  B01J20/285 ,  B01D15/34 ,  C07K16/00 ,  B01J20/286

CPC classification number: C07K1/165 ,  B01D15/1807 ,  B01D15/1864 ,  B01D15/34 ,  B01J20/285 ,  B01J20/286 ,  C07K1/22 ,  C07K14/31 ,  C07K16/00 ,  C07K2317/21 ,  C07K2317/31 ,  C07K2319/30

Abstract: The invention relates to a method of isolating an immunoglobulin, comprising the steps of: a) providing a separation matrix comprising at least 15 mg/ml multimers of immunoglobulin-binding alkali-stabilized Protein A domains covalently coupled to a porous support, wherein the porous support comprises cross-linked polymer particles having a volume-weighted median diameter (d50,v) of 56-70 micrometers and a dry solids weight of 55-80 mg/ml; b) contacting a liquid sample comprising an immunoglobulin with the separation matrix; c) washing the separation matrix with a washing liquid; d) eluting the immunoglobulin from the separation matrix with an elution liquid; and e) cleaning the separation matrix with a cleaning liquid comprising at least 0.5 M NaOH.

公开(公告)号：US20180043284A1

公开(公告)日：2018-02-15

申请号：US15793923

申请日：2017-10-25

Applicant: GE Healthcare BioProcess R&D AB

Inventor： KLAUS GEBAUER

IPC: B01D15/14 ,  G01N30/60 ,  B01D15/22 ,  G01N30/46 ,  B01D15/18 ,  G01N30/88

CPC classification number: B01D15/14 ,  B01D15/1864 ,  B01D15/1885 ,  B01D15/22 ,  G01N30/466 ,  G01N30/6039 ,  G01N30/6043 ,  G01N30/6091 ,  G01N2030/8881 ,  Y10T29/49826

Abstract: A parallel assembly (2; 11; 51) of chromatography column modules (3a,b,c; 13a,b,c; 53a,b,c, 90a, b), the assembly having one common assembly inlet (15; 55) and one common assembly outlet (17; 57), each column module comprising a bed space (29) filled with chromatography medium and each column module comprises integrated fluid conduits which when the column module is connected with other column modules are adapted to connect the bed space (29) of the column module with the assembly inlet (15; 55) and the assembly outlet (17; 57), wherein the total length and/or volume of the fluid conduit from the assembly inlet to one bed space together with the length and/or volume of the fluid conduit from the same bed space to the assembly outlet is substantially the same for all bed spaces and modules installed in the parallel assembly.