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    Methods and apparatus for measuring analytes using large scale FET arrays
    43.
    发明授权
    Methods and apparatus for measuring analytes using large scale FET arrays 有权

    公开(公告)号：US11530444B2

    公开(公告)日：2022-12-20

    申请号：US16882414

    申请日：2020-05-22

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Jonathan M. Rothberg ,  Wolfgang Hinz ,  Kim L. Johnson ,  James Bustillo ,  John Leamon ,  Jonathan Schultz

    IPC: C12Q1/6874 ,  G01N27/414 ,  B01L3/00 ,  C12Q1/6869

    Abstract: Methods and apparatus relating to very large scale FET arrays for analyte measurements. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes. In one example, chemFET arrays facilitate DNA sequencing techniques based on monitoring changes in the concentration of inorganic pyrophosphate (PPi), hydrogen ions, and nucleotide triphosphates.

    Fluid interface to receive removable container
    45.
    发明授权
    Fluid interface to receive removable container 有权

    公开(公告)号：US11214478B2

    公开(公告)日：2022-01-04

    申请号：US17165196

    申请日：2021-02-02

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Todd Roswech ,  Jonathan Schultz ,  Chun Ho

    IPC: B67D7/02 ,  B67D7/76 ,  B01L3/00 ,  G01N35/10

    Abstract: A fluidic interconnect includes a first interface including a liquid port, a gas port, and a cradle; a second interface including a liquid port, a gas port, and a swing bar to engage the cradle, a weight of a container attached to one of the first or second interfaces to drive the liquid port of the first interface into connection with the liquid port of the second interface and the gas port of the first interface into connection with the gas port of the second interface.

    Fluid interface to receive removable container
    47.
    发明授权
    Fluid interface to receive removable container 有权

    公开(公告)号：US10427928B2

    公开(公告)日：2019-10-01

    申请号：US15259245

    申请日：2016-09-08

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Todd Roswech ,  Jonathan Schultz ,  Chun Ho

    IPC: B67D7/76 ,  B01L3/00 ,  B67D7/02 ,  G01N35/10

    Abstract: A fluidic interconnect includes a first interface including a liquid port, a gas port, and a cradle; a second interface including a liquid port, a gas port, and a swing bar to engage the cradle, a weight of a container attached to one of the first or second interfaces to drive the liquid port of the first interface into connection with the liquid port of the second interface and the gas port of the first interface into connection with the gas port of the second interface.

    Alternative nucleotide flows in sequencing-by-synthesis methods
    48.
    发明授权
    Alternative nucleotide flows in sequencing-by-synthesis methods 有权

    公开(公告)号：US10392660B2

    公开(公告)日：2019-08-27

    申请号：US15404735

    申请日：2017-01-12

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Jonathan Schultz ,  John Davidson

    IPC: B01L3/00 ,  C12Q1/6869 ,  C12Q1/6874

    Abstract: A method of sequencing a polynucleotide strand can include providing the polynucleotide strand with a primer annealed thereto and a polymerase operably bound to the polynucleotide strand; successively exposing the polynucleotide strand to the flow of four different dNTPs according to a first predetermined ordering; and successively exposing the polynucleotide strand to the flow of four different dNTPs according to a second predetermined ordering, wherein the second predetermined ordering is different from the first predetermined ordering.

    Phase-protecting reagent flow ordering for use in sequencing-by-synthesis
    49.
    发明授权
    Phase-protecting reagent flow ordering for use in sequencing-by-synthesis 有权

    公开(公告)号：US10370708B2

    公开(公告)日：2019-08-06

    申请号：US15236172

    申请日：2016-08-12

    Applicant: Life Technologies Corporation

    Inventor： Earl Hubbell ,  Jonathan Schultz

    IPC: C12Q1/68 ,  C12Q1/6869 ,  C12Q1/6874

    Abstract: A system and machine readable medium for nucleic acid sequencing includes disposing template polynucleotide strands in defined spaces disposed on a sensor array, at least some of the template polynucleotide strands having a sequencing primer and a polymerase operably bound therewith; exposing the template polynucleotide strands to a series of flows of nucleotide species flowed according to a predetermined ordering; and determining, for each of the series of flows of nucleotide species, how many nucleotide incorporations occurred for that particular flow to determine a predicted sequence of nucleotides corresponding to the template polynucleotide strands, wherein the predetermined ordering (a) is not a series of consecutive repetitions of a 4-flow permutation of four different nucleotide species, (b) is not specifically tailored to a particular combination of a particular template polynucleotide strand to be sequenced and a particular sequencing primer to be used, and (c) comprises a phase-protecting flow ordering.

    Sequencing device
    50.
    发明授权
    Sequencing device 有权

    公开(公告)号：US10240193B2

    公开(公告)日：2019-03-26

    申请号：US15891485

    申请日：2018-02-08

    Applicant: Life Technologies Corporation

    Inventor： Jonathan Schultz ,  Todd Roswech ,  Jon A. Hoshizaki ,  Albert L. Carrillo ,  James A. Ball

    IPC: C12Q1/68 ,  B01L3/00 ,  G01N27/414 ,  G01N35/10 ,  B01F11/00 ,  B01F1/00 ,  B01F5/06 ,  C12Q1/6869 ,  C12Q1/6806

    Abstract: A method of preparing reagents includes inserting a cartridge into an instrument. The cartridge includes a plurality of reagent enclosures disposed in a cavity of the cartridge and exposing a port to an exterior of the cartridge. Each reagent enclosure includes a reagent container including a reagent and an internal cavity defining a compressible volume, an opening defined through the reagent container to the internal cavity. The method further includes connecting a plurality of fluid ports to the openings of the plurality of reagent enclosures; applying a solution through the fluid ports to at least partially fill the plurality of reagent enclosures; and cycling a pressure of the cavity, whereby for each of the reagent enclosures, during increasing pressure, the solution enters the internal cavity of the reagent container, combines with the reagent, and compresses the compressible volume, and during decreasing pressure, the compressible volume decreases and the reagent is ejected through the opening.

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