公开(公告)号：US20160068900A1

公开(公告)日：2016-03-10

申请号：US14773366

申请日：2014-03-15

申请人： Lyle J. ARNOLD ,  Norman C. NELSON

发明人： Lyle J. Arnold ,  Norman C. Nelson

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6858 ,  C12Q1/6806 ,  C12Q1/6844 ,  C12Q2525/161 ,  C12Q2525/186 ,  C12Q2525/204 ,  C12Q2531/101

摘要： The present invention provides methods of amplifying a fragmented target nucleic acid containing short target nucleic acid fragments utilizing an assembler sequence to convert these short fragments into longer sequences enabling their identification and interrogation. This is particularly important when attempting to identify small genetic variations, such as SNVs, present in highly fragmented nucleic acid samples. Amplification is accomplished by hybridizing the short target nucleic acid sequences to the assembler sequence, where these short sequences serve as primers for extension. Since the fragmented target nucleic acids that contain SNVs are utilized as primers on the assembler sequence they are preserved during amplification and can be detected.

摘要翻译： 本发明提供了利用汇编序列扩增含有短靶核酸片段的片段化靶核酸的方法，以将这些短片段转化成更长的序列，使其能够进行鉴定和询问。 当试图鉴定存在于高度片段化的核酸样品中的小的遗传变异（例如SNV）时，这尤其重要。 通过将短目标核酸序列杂交到汇编序列来完成扩增，其中这些短序列用作扩增的引物。 由于含有SNV的片段化靶核酸作为汇编序列上的引物，因此在扩增过程中被保留并可以被检测。

公开(公告)号：US20160024546A1

公开(公告)日：2016-01-28

申请号：US14773363

申请日：2014-03-14

申请人： Lyle J. Arnold ,  Norman C. Nelson

发明人： Lyle J. Arnold ,  Norman C. Nelson

IPC分类号： C12P19/34

CPC分类号： C12P19/34 ,  C12Q1/6844 ,  C12Q2531/119 ,  C12Q2531/125 ,  C12Q2537/162

摘要： The present invention provides methods of amplifying a target nucleic acid utilizing a circularized template. Circularization may be achieved utilizing a bridging oligonucleotide or an inverter primer. The bridging oligonucleotide or inverted primer is extended forming a concatemeric amplicon that can then be used as a template to provide exponential amplification of the target nucleic acid.

摘要翻译： 本发明提供利用环化模板扩增靶核酸的方法。 可以使用桥连寡核苷酸或反向引物实现环化。 桥接寡核苷酸或反向引物延伸形成一个可以用作模板以提供靶核酸的指数扩增的并联扩增子。

公开(公告)号：US08765652B2

公开(公告)日：2014-07-01

申请号：US13531924

申请日：2012-06-25

申请人： Norman C. Nelson ,  Kenneth A. Browne ,  Lizhong Dai ,  James Russell ,  Mark E. Filipowsky ,  Margarita B. Kaminsky ,  Daniel L. Kacian

发明人： Norman C. Nelson ,  Kenneth A. Browne ,  Lizhong Dai ,  James Russell ,  Mark E. Filipowsky ,  Margarita B. Kaminsky ,  Daniel L. Kacian

IPC分类号： C11D3/20 ,  C11D3/39 ,  C09K3/00

CPC分类号： C12Q1/68 ,  A61L2/186 ,  C11D3/3956 ,  C12Q1/6848

摘要： The present invention relates to reagents for use in deactivating nucleic acids and methods of making and using the same.

摘要翻译： 本发明涉及用于失活核酸的试剂及其制备和使用方法。

公开(公告)号：US08491178B2

公开(公告)日：2013-07-23

申请号：US13414195

申请日：2012-03-07

申请人： Scott S. Breidenthal ,  Sara H. Fan ,  Richard S. Lee ,  Norman C. Nelson ,  Matthew J. Scott ,  Jason A. Taylor

发明人： Scott S. Breidenthal ,  Sara H. Fan ,  Richard S. Lee ,  Norman C. Nelson ,  Matthew J. Scott ,  Jason A. Taylor

IPC分类号： B01F15/00 ,  B01F11/00 ,  G01N21/01

CPC分类号： B01F11/00 ,  B01F3/1271 ,  B01F11/0045 ,  B01F11/0071 ,  B01F13/0059 ,  B01F15/00214 ,  B01F2215/0037 ,  B01L3/502 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502761 ,  B01L7/52 ,  B01L7/525 ,  B01L2200/0621 ,  B01L2200/0647 ,  B01L2200/0673 ,  B01L2200/0689 ,  B01L2200/10 ,  B01L2200/14 ,  B01L2200/16 ,  B01L2300/0816 ,  B01L2300/087 ,  B01L2300/0887 ,  B01L2300/123 ,  B01L2300/1805 ,  B01L2300/1822 ,  B01L2300/1883 ,  B01L2400/0457 ,  B01L2400/0478 ,  B01L2400/0481 ,  B01L2400/0655 ,  B01L2400/0677 ,  C12N15/1006 ,  C12Q1/6806 ,  C12Q1/6844 ,  G01N1/44 ,  G01N21/01 ,  G01N21/6428 ,  G01N21/645 ,  G01N35/0098 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2021/6432 ,  G01N2021/6439 ,  G01N2021/6463 ,  G01N2035/00544 ,  G01N2201/0627 ,  Y10T137/0391 ,  Y10T436/143333 ,  Y10T436/25 ,  Y10T436/255

摘要： A receptacle having interconnected chambers arranged to permit multiple process steps to be performed independently or simultaneously. The receptacles are manufactured to separate liquid from dried reagents and to maintain the stability of the dried reagents. An immiscible liquid, such as an oil, is included to control loading of process materials, facilitate mixing and reconstitution of dried reagents, limit evaporation, control heating of reaction materials, concentrate solid support materials to prevent clogging of fluid connections, provide minimum volumes for fluid transfers, and to prevent process materials from sticking to chamber surfaces. The receptacles can be adapted for use in systems having a processing instrument that includes an actuator system for selectively moving fluid substances between chambers and a detector. The actuator system can be arranged to concentrate an analyte present in a sample. The detector can be used to detect an optical signal emitted by the contents of the receptacle.

摘要翻译： 具有互连室的容器，其布置成允许独立地或同时地执行多个工艺步骤。 制造容器以将液体与干燥试剂分开并保持干燥试剂的稳定性。 包括不混溶的液体，如油，以控制加工材料的加载，促进干燥试剂的混合和重构，限制蒸发，控制反应材料的加热，浓缩固体支持材料以防止流体连接堵塞，为 流体转移，并防止工艺材料粘附到室表面。 插座可以适用于具有处理器具的系统，该处理器具包括用于选择性地移动腔室和检测器之间的流体物质的致动器系统。 致动器系统可以被布置成集中存在于样品中的分析物。 检测器可用于检测由容器的内容物发射的光信号。

公开(公告)号：US20120211088A1

公开(公告)日：2012-08-23

申请号：US13215081

申请日：2011-08-22

申请人： Norman C. Nelson ,  Gianluca Roma

发明人： Norman C. Nelson ,  Gianluca Roma

IPC分类号： F17D1/18

CPC分类号： B01F11/00 ,  B01F3/1271 ,  B01F11/0045 ,  B01F11/0071 ,  B01F13/0059 ,  B01F15/00214 ,  B01F2215/0037 ,  B01L3/502 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502761 ,  B01L7/52 ,  B01L7/525 ,  B01L2200/0621 ,  B01L2200/0647 ,  B01L2200/0673 ,  B01L2200/0689 ,  B01L2200/10 ,  B01L2200/14 ,  B01L2200/16 ,  B01L2300/0816 ,  B01L2300/087 ,  B01L2300/0887 ,  B01L2300/123 ,  B01L2300/1805 ,  B01L2300/1822 ,  B01L2300/1883 ,  B01L2400/0457 ,  B01L2400/0478 ,  B01L2400/0481 ,  B01L2400/0655 ,  B01L2400/0677 ,  C12N15/1006 ,  C12Q1/6806 ,  C12Q1/6844 ,  G01N1/44 ,  G01N21/01 ,  G01N21/6428 ,  G01N21/645 ,  G01N35/0098 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2021/6432 ,  G01N2021/6439 ,  G01N2021/6463 ,  G01N2035/00544 ,  G01N2201/0627 ,  Y10T137/0391 ,  Y10T436/143333 ,  Y10T436/25 ,  Y10T436/255

摘要： A receptacle having a plurality of interconnected chambers arranged to permit multiple process steps or processes to be performed independently or simultaneously. The receptacles are manufactured to separate liquid from dried reagents and to maintain the stability of the dried reagents. An immiscible liquid, such as an oil, is included to control loading of process materials, facilitate mixing and reconstitution of dried reagents, limit evaporation, control heating of reaction materials, concentrate solid support materials to prevent clogging of fluid connections, provide minimum volumes for fluid transfers, and to prevent process materials from sticking to chamber surfaces. The receptacles can be adapted for use in systems having a processing instrument that includes an actuator system for selectively moving fluid substances between chambers and a detector. The actuator system can be arranged to concentrate an analyte present in a sample. The detector can be used to detect an optical signal emitted by the contents of the receptacle.

公开(公告)号：US08221705B2

公开(公告)日：2012-07-17

申请号：US12337108

申请日：2008-12-17

申请人： Scott S. Breidenthal ,  Daniel L. Kacian ,  Richard S. Lee ,  Norman C. Nelson ,  Matthew J. Scott ,  Jason A. Taylor

发明人： Scott S. Breidenthal ,  Daniel L. Kacian ,  Richard S. Lee ,  Norman C. Nelson ,  Matthew J. Scott ,  Jason A. Taylor

IPC分类号： G01N33/00

CPC分类号： B01F11/00 ,  B01F3/1271 ,  B01F11/0045 ,  B01F11/0071 ,  B01F13/0059 ,  B01F15/00214 ,  B01F2215/0037 ,  B01L3/502 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502761 ,  B01L7/52 ,  B01L7/525 ,  B01L2200/0621 ,  B01L2200/0647 ,  B01L2200/0673 ,  B01L2200/0689 ,  B01L2200/10 ,  B01L2200/14 ,  B01L2200/16 ,  B01L2300/0816 ,  B01L2300/087 ,  B01L2300/0887 ,  B01L2300/123 ,  B01L2300/1805 ,  B01L2300/1822 ,  B01L2300/1883 ,  B01L2400/0457 ,  B01L2400/0478 ,  B01L2400/0481 ,  B01L2400/0655 ,  B01L2400/0677 ,  C12N15/1006 ,  C12Q1/6806 ,  C12Q1/6844 ,  G01N1/44 ,  G01N21/01 ,  G01N21/6428 ,  G01N21/645 ,  G01N35/0098 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2021/6432 ,  G01N2021/6439 ,  G01N2021/6463 ,  G01N2035/00544 ,  G01N2201/0627 ,  Y10T137/0391 ,  Y10T436/143333 ,  Y10T436/25 ,  Y10T436/255

摘要： A receptacle having a plurality of interconnected chambers arranged to permit multiple process steps or processes to be performed independently or simultaneously. The receptacles are manufactured to separate liquid from dried reagents and to maintain the stability of the dried reagents. An immiscible liquid, such as an oil, is included to control loading of process materials, facilitate mixing and reconstitution of dried reagents, limit evaporation, control heating of reaction materials, concentrate solid support materials to prevent clogging of fluid connections, provide minimum volumes for fluid transfers, and to prevent process materials from sticking to chamber surfaces. The receptacles can be adapted for use in systems having a processing instrument that includes an actuator system for selectively moving fluid substances between chambers and a detector. The actuator system can be arranged to concentrate an analyte present in a sample. The detector can be used to detect an optical signal emitted by the contents of the receptacle.

摘要翻译： 具有多个互连室的容器，其布置成允许独立地或同时地执行多个工艺步骤或工艺。 制造容器以将液体与干燥试剂分开并保持干燥试剂的稳定性。 包括不混溶的液体，如油，以控制加工材料的加载，促进干燥试剂的混合和重构，限制蒸发，控制反应材料的加热，浓缩固体支持材料以防止流体连接堵塞，为 流体转移，并防止工艺材料粘附到室表面。 插座可以适用于具有处理器具的系统，该处理器具包括用于选择性地移动腔室和检测器之间的流体物质的致动器系统。 致动器系统可以被布置成集中存在于样品中的分析物。 检测器可用于检测由容器的内容物发射的光信号。

公开(公告)号：US08048375B2

公开(公告)日：2011-11-01

申请号：US12333335

申请日：2008-12-12

申请人： Scott S. Breidenthal ,  Richard S. Lee ,  Norman C. Nelson ,  Matthew J. Scott ,  Jason A. Taylor ,  Sara H. Fan

发明人： Scott S. Breidenthal ,  Richard S. Lee ,  Norman C. Nelson ,  Matthew J. Scott ,  Jason A. Taylor ,  Sara H. Fan

IPC分类号： G01N15/06

CPC分类号： B01F11/00 ,  B01F3/1271 ,  B01F11/0045 ,  B01F11/0071 ,  B01F13/0059 ,  B01F15/00214 ,  B01F2215/0037 ,  B01L3/502 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502761 ,  B01L7/52 ,  B01L7/525 ,  B01L2200/0621 ,  B01L2200/0647 ,  B01L2200/0673 ,  B01L2200/0689 ,  B01L2200/10 ,  B01L2200/14 ,  B01L2200/16 ,  B01L2300/0816 ,  B01L2300/087 ,  B01L2300/0887 ,  B01L2300/123 ,  B01L2300/1805 ,  B01L2300/1822 ,  B01L2300/1883 ,  B01L2400/0457 ,  B01L2400/0478 ,  B01L2400/0481 ,  B01L2400/0655 ,  B01L2400/0677 ,  C12N15/1006 ,  C12Q1/6806 ,  C12Q1/6844 ,  G01N1/44 ,  G01N21/01 ,  G01N21/6428 ,  G01N21/645 ,  G01N35/0098 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2021/6432 ,  G01N2021/6439 ,  G01N2021/6463 ,  G01N2035/00544 ,  G01N2201/0627 ,  Y10T137/0391 ,  Y10T436/143333 ,  Y10T436/25 ,  Y10T436/255

摘要： A receptacle having a plurality of interconnected chambers arranged to permit multiple process steps or processes to be performed independently or simultaneously. The receptacles are manufactured to separate liquid from dried reagents and to maintain the stability of the dried reagents. An immiscible liquid, such as an oil, is included to control loading of process materials, facilitate mixing and reconstitution of dried reagents, limit evaporation, control heating of reaction materials, concentrate solid support materials to prevent clogging of fluid connections, provide minimum volumes for fluid transfers, and to prevent process materials from sticking to chamber surfaces. The receptacles can be adapted for use in systems having a processing instrument that includes an actuator system for selectively moving fluid substances between chambers and a detector.

公开(公告)号：US07696337B2

公开(公告)日：2010-04-13

申请号：US11574307

申请日：2005-08-26

申请人： Michael M. Becker ,  Steven T. Brentano ,  Daniel P. Kolk ,  Wai-Chung Lam ,  Kristin W. Livezey ,  Norman C. Nelson ,  Astrid R. W. Schroder ,  Gary P. Schroth

发明人： Michael M. Becker ,  Steven T. Brentano ,  Daniel P. Kolk ,  Wai-Chung Lam ,  Kristin W. Livezey ,  Norman C. Nelson ,  Astrid R. W. Schroder ,  Gary P. Schroth

IPC分类号： C07H21/04 ,  C12Q1/68

CPC分类号： C12P19/34 ,  C12Q1/6865 ,  C12Q2537/163 ,  C12Q2521/325 ,  C12Q2521/107 ,  C12Q2525/186 ,  C12Q2533/101 ,  C12Q2525/143

摘要： The present invention is directed to novel methods of synthesizing multiple copies of a target nucleic acid sequence which are autocatalytic (i.e., able to cycle automatically without the need to modify reaction conditions such as temperature, pH, or ionic strength and using the product of one cycle in the next one). In particular, the present invention discloses a method of nucleic acid amplification which is robust and efficient, while reducing the appearance of side products. The method uses only one primer, the “priming oligonucleotide,” a 3′blocked promoter oligonucleotide and optionally, a means for terminating a primer extension reaction, to amplify RNA or DNA molecules in vitro, while reducing or eliminating the formation of side products. The method of the present invention minimizes or eliminates the emergence of side products, thus providing a high level of specificity. Furthermore, the appearance of side products can complicate the analysis of the amplification reaction by various molecular detection techniques. The present invention minimizes or eliminates this problem, thus providing an enhanced level of sensitivity.

摘要翻译： 本发明涉及合成目标核酸序列的多个拷贝的新型方法，所述目标核酸序列是自动催化的（即，能够自动循环而不需要修饰诸如温度，pH或离子强度的反应条件，并且使用一种 循环在下一个）。 特别地，本发明公开了一种鲁棒有效的核酸扩增方法，同时减少了副产物的外观。 该方法仅使用一种引物，即引物寡核苷酸，3'封闭的启动子寡核苷酸和任选的终止引物延伸反应的手段，以体外扩增RNA或DNA分子，同时减少或消除副产物的形成。 本发明的方法使副产物的出现最小化或消除，从而提供高水平的特异性。 此外，副产物的出现可能通过各种分子检测技术使扩增反应的分析复杂化。 本发明使这个问题最小化或消除了这一点，从而提供了增强的灵敏度。

公开(公告)号：US20090139992A1

公开(公告)日：2009-06-04

申请号：US12337108

申请日：2008-12-17

申请人： Scott S. Breidenthal ,  Daniel L. Kacian ,  Richard S. Lee ,  Norman C. Nelson ,  Matthew J. Scott ,  Jason A. Taylor

发明人： Scott S. Breidenthal ,  Daniel L. Kacian ,  Richard S. Lee ,  Norman C. Nelson ,  Matthew J. Scott ,  Jason A. Taylor

IPC分类号： B65D25/04

CPC分类号： B01F11/00 ,  B01F3/1271 ,  B01F11/0045 ,  B01F11/0071 ,  B01F13/0059 ,  B01F15/00214 ,  B01F2215/0037 ,  B01L3/502 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502761 ,  B01L7/52 ,  B01L7/525 ,  B01L2200/0621 ,  B01L2200/0647 ,  B01L2200/0673 ,  B01L2200/0689 ,  B01L2200/10 ,  B01L2200/14 ,  B01L2200/16 ,  B01L2300/0816 ,  B01L2300/087 ,  B01L2300/0887 ,  B01L2300/123 ,  B01L2300/1805 ,  B01L2300/1822 ,  B01L2300/1883 ,  B01L2400/0457 ,  B01L2400/0478 ,  B01L2400/0481 ,  B01L2400/0655 ,  B01L2400/0677 ,  C12N15/1006 ,  C12Q1/6806 ,  C12Q1/6844 ,  G01N1/44 ,  G01N21/01 ,  G01N21/6428 ,  G01N21/645 ,  G01N35/0098 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2021/6432 ,  G01N2021/6439 ,  G01N2021/6463 ,  G01N2035/00544 ,  G01N2201/0627 ,  Y10T137/0391 ,  Y10T436/143333 ,  Y10T436/25 ,  Y10T436/255

摘要： A receptacle having a plurality of interconnected chambers arranged to permit multiple process steps or processes to be performed independently or simultaneously. The receptacles are manufactured to separate liquid from dried reagents and to maintain the stability of the dried reagents. An immiscible liquid, such as an oil, is included to control loading of process materials, facilitate mixing and reconstitution of dried reagents, limit evaporation, control heating of reaction materials, concentrate solid support materials to prevent clogging of fluid connections, provide minimum volumes for fluid transfers, and to prevent process materials from sticking to chamber surfaces. The receptacles can be adapted for use in systems having a processing instrument that includes an actuator system for selectively moving fluid substances between chambers and a detector. The actuator system can be arranged to concentrate an analyte present in a sample. The detector can be used to detect an optical signal emitted by the contents of the receptacle.

摘要翻译： 具有多个互连室的容器，其布置成允许独立地或同时地执行多个工艺步骤或工艺。 制造容器以将液体与干燥试剂分开并保持干燥试剂的稳定性。 包括不混溶的液体，如油，以控制加工材料的加载，促进干燥试剂的混合和重构，限制蒸发，控制反应材料的加热，浓缩固体支持材料以防止流体连接堵塞，为 流体转移，并防止工艺材料粘附到室表面。 插座可以适用于具有处理器具的系统，该处理器具包括用于选择性地移动腔室和检测器之间的流体物质的致动器系统。 致动器系统可以被布置成集中存在于样品中的分析物。 检测器可用于检测由容器的内容物发射的光信号。

公开(公告)号：US06350579B1

公开(公告)日：2002-02-26

申请号：US09649636

申请日：2000-08-28

申请人： Norman C. Nelson

发明人： Norman C. Nelson

IPC分类号： G01N33533

CPC分类号： G01N33/54306 ,  C12Q1/6816 ,  Y10S435/962 ,  Y10S435/968 ,  Y10S436/80 ,  Y10S436/814 ,  Y10S436/815 ,  Y10S436/817

摘要： A method and compositions for the detection and/or quantification of an analyte through the use of a plurality of labeled probes, with two or more said probes targeted to different regions of said analyte. In specific embodiments, the labels are separately distinguishable, and/or are present at different specific activities on the labels.

摘要翻译： 一种用于通过使用多个标记探针来检测和/或定量分析物的方法和组合物，其中两个或多个所述探针靶向所述分析物的不同区域。 在具体实施方案中，标签是分开区分的，和/或以不同的特定活性存在于标签上。