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122 条记录 (耗时 0.058 秒)

    Apparatus and methods for simultaneous operation of miniaturized reactors
    42.
    发明授权
    Apparatus and methods for simultaneous operation of miniaturized reactors 失效
    用于小型化反应堆同时操作的装置和方法

    公开(公告)号：US07507579B2

    公开(公告)日：2009-03-24

    申请号：US10816046

    申请日：2004-04-01

    申请人： Paolo Boccazzi ,  Angela Y. Chen ,  Klavs F. Jensen ,  Nicolas Szita ,  Andrea Zanzotto ,  Zhiyu Zhang

    发明人： Paolo Boccazzi ,  Angela Y. Chen ,  Klavs F. Jensen ,  Nicolas Szita ,  Andrea Zanzotto ,  Zhiyu Zhang

    IPC分类号： C12M1/12 ,  C12M1/34 ,  C12M3/00 ,  C12M1/00 ,  G06K9/00

    CPC分类号： C12M1/12 ,  B01F13/0059 ,  B01F13/0827 ,  B01J2219/00283 ,  B01J2219/00317 ,  B01J2219/00479 ,  B01J2219/00481 ,  B01J2219/00484 ,  B01J2219/00495 ,  B01J2219/00585 ,  B01J2219/00599 ,  B01J2219/00691 ,  B01J2219/00743 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/502723 ,  B01L9/527 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2300/0877 ,  B01L2300/10 ,  B01L2400/0466 ,  B01L2400/0487 ,  C12M23/16 ,  C12M35/08 ,  C12M41/48 ,  G01N2035/00326 ,  Y10S435/808 ,  Y10S435/809

    摘要： The present invention provides a variety of microscale bioreactors (microfermentors) and microscale bioreactor arrays for use in culturing cells. The microfermentors include a vessel for culturing cells and means for providing oxygen to the interior of the vessel at a concentration sufficient to support cell growth, e.g., growth of bacterial cells. Depending on the embodiment, the microfermentor vessel may have various interior volumes less than approximately 1 ml. The microfermentors may include an aeration membrane and optionally a variety of sensing devices. The invention further provides a chamber to contain the microfermentors and microfermentor arrays and to provide environmental control. Certain of the microfermentors include a second chamber that may be used, e.g., to provide oxygen, nutrients, pH control, etc., to the culture vessel and/or to remove metabolites, etc. Various methods of using the microfermentors, e.g., to select optimum cell strains or bioprocess parameters are provided. The invention provides microreactors having a variety of different designs, some of which incorporate active stirring and/or have the capability to operate in batch or fed-batch mode. The invention further provides an apparatus and methods for simultaneous operation of a plurality of microreactors, with monitoring of the individual microreactors during a run. The invention further provides methods of performing gene expression analysis on cells cultured in microreactors.

    摘要翻译： 本发明提供用于培养细胞的各种微量生物反应器（微发酵器）和微生物生物反应器阵列。 微发酵器包括用于培养细胞的容器和用于以足以支持细胞生长（例如细菌细胞生长）的浓度向容器内部提供氧气的装置。 根据实施方案，微发酵罐容器可以具有小于约1ml的各种内部容积。 微发酵器可以包括曝气膜和任选的各种感测装置。 本发明进一步提供了容纳微发射体和微发光体阵列并提供环境控制的腔室。 某些微发酵器包括第二室，其可以用于例如向培养容器提供氧气，营养物质，pH控制等，和/或去除代谢物等。使用微发酵器的各种方法，例如， 选择最佳细胞株或生物过程参数。 本发明提供具有各种不同设计的微反应器，其中一些具有主动搅拌和/或具有以分批或分批模式操作的能力。 本发明还提供了一种用于同时操作多个微反应器的装置和方法，其中在运行期间监测各个微反应器。 本发明还提供了对在微反应器中培养的细胞进行基因表达分析的方法。

    Pumping and flow control in systems including microfluidic systems
    49.
    发明授权
    Pumping and flow control in systems including microfluidic systems 有权
    在包括微流体系统的系统中的泵送和流量控制

    公开(公告)号：US08557570B2

    公开(公告)日：2013-10-15

    申请号：US12312348

    申请日：2007-11-06

    申请人： Hemantkumar R. Sahoo ,  Klavs F. Jensen

    发明人： Hemantkumar R. Sahoo ,  Klavs F. Jensen

    IPC分类号： C12M1/00 ,  C12M3/00

    CPC分类号： B01L3/50273 ,  B01L2200/146 ,  B01L2300/0816 ,  B01L2300/14 ,  B01L2400/0487 ,  Y10T137/0396 ,  Y10T137/86485

    摘要： The present invention generally relates to devices and methods for affecting the flow rate of fluid using pressure. The invention generally provides for controlled application of pressure to flowing fluids to control pressure and flow rates of those fluids, independent of location of the fluids relative to various devices. For example, in a series of devices, each connected to another via a conduit, pressure control units can be provided between devices to raise or lower pressure and/or flow rate of fluid flowing from one device to the next. In this way, a series of interconnected devices can be arranged such that inlet fluid pressure or flow rate of any individual device can be set independently of every other device.

    摘要翻译： 本发明一般涉及使用压力影响流体流速的装置和方法。 本发明通常提供对流动流体的压力的受控应用，以控制这些流体的压力和流速，而与流体相对于各种装置的位置无关。 例如，在一系列装置中，每个装置通过管道连接到另一个装置，压力控制单元可以设置在装置之间以提高或降低从一个装置流向下一个装置的流体的压力和/或流速。 以这种方式，可以布置一系列互连的装置，使得任何单独装置的入口流体压力或流量可以独立于每个其他装置设定。

    Small-scale method and apparatus for separating mixtures
    50.
    发明授权
    Small-scale method and apparatus for separating mixtures 有权
    用于分离混合物的小规模方法和装置

    公开(公告)号：US08435387B2

    公开(公告)日：2013-05-07

    申请号：US12617984

    申请日：2009-11-13

    申请人： Ryan L. Hartman ,  Hemantkumar R. Sahoo ,  Klavs F. Jensen

    发明人： Ryan L. Hartman ,  Hemantkumar R. Sahoo ,  Klavs F. Jensen

    IPC分类号： B01D1/0041 ,  B01D3/009 ,  B01J19/0093

    CPC分类号： B01J19/0093 ,  B01D1/0088 ,  B01D1/14 ,  B01J2219/00783 ,  B01J2219/00822 ,  B01J2219/00824 ,  B01J2219/00831 ,  B01J2219/00833 ,  B01J2219/00837 ,  B01J2219/0086 ,  B01J2219/00867 ,  B01J2219/00873 ,  B01J2219/00891 ,  B01J2219/00932

    摘要： The present invention generally relates to the small-scale separation of a mixture of two or more components with different boiling points into enriched fractions. In some embodiments, a first and second fluid (e.g., a liquid and a gas, a liquid and a liquid, etc.) are passed through a channel. The first fluid may include at least two components, each with a unique boiling point. Upon contacting the first and second fluids within the channel, at least a portion of the most volatile of the components in the first fluid (i.e., the component with the lowest boiling point) may be transferred from the first fluid to the second fluid. In some instances, the transfer of the volatile component(s) from the first fluid to the second fluid may be expedited by heating, in some cases above the boiling point(s) of the component(s) to be transferred from the first fluid to the second fluid. Contact between the first and second fluids may be maintained, for example, via segmented flow, bubbling flow, etc. In some instances, separation between the first and second fluids may be maintained in a channel that is essentially free of interior microchannel surface irregularities.

    摘要翻译： 本发明一般涉及将具有不同沸点的两种或多种组分的混合物小规模分离成富集级分。 在一些实施例中，第一和第二流体（例如液体和气体，液体和液体等）通过通道。 第一流体可以包括至少两个组分，每个组分具有独特的沸点。 在通道内接触第一和第二流体时，第一流体（即，具有最低沸点的组分）中最易挥发的组分的至少一部分可以从第一流体转移到第二流体。 在一些情况下，挥发性组分从第一流体转移到第二流体可以通过加热，在一些情况下高于待从第一流体转移的组分的沸点 到第二流体。 第一和第二流体之间的接触可以例如通过分段流动，起泡流等来保持。在一些情况下，第一和第二流体之间的分离可保持在基本上没有内部微通道表面不规则的通道中。