公开(公告)号：US20060135452A1

公开(公告)日：2006-06-22

申请号：US10536274

申请日：2003-12-02

申请人： Kazuya Hiraoka ,  Seiji Yamamoto ,  Yasufumi Kaneda ,  Ryuichi Morishita ,  Toshio Ogihara

发明人： Kazuya Hiraoka ,  Seiji Yamamoto ,  Yasufumi Kaneda ,  Ryuichi Morishita ,  Toshio Ogihara

IPC分类号： A61K48/00

CPC分类号： C07K14/475 ,  A61K48/0016 ,  A61K48/0083 ,  C07K14/4753 ,  C12N9/0073

摘要： A method for enhancing the transfection efficiency of naked plasmid DNA in treating and/or preventing angiogenesis-dependent symptoms is provided by the present inventions. According to the present method, a suitable naked plasmid DNA is subjected for intramuscular injection under increased pressure inside the muscle or hyperbaric oxygen. Angiogenesis-dependent symptoms, including wounds, inflammatory diseases, critical limb ischemia, ischemic heart diseases, cerebral infarction, diabetic neuropathy, spinal canal stenosis, etc., may be treated by the present methods.

摘要翻译： 本发明提供了一种提高裸小质粒DNA在治疗和/或预防血管生成依赖性症状中的转染效率的方法。 根据本方法，将合适的裸质粒DNA在肌肉或高压氧内加压肌内注射。 可以通过本发明的方法治疗血管发生依赖性症状，包括伤口，炎性疾病，临界肢体缺血，缺血性心脏病，脑梗塞，糖尿病性神经病变，椎管狭窄等。

公开(公告)号：US20060135449A1

公开(公告)日：2006-06-22

申请号：US10509799

申请日：2002-03-29

申请人： Yoshiki Sawa ,  Ryuichi Morishita ,  Yasufumi Kaneda ,  Hikaru Matsuda ,  Toshiki Yoshimine

发明人： Yoshiki Sawa ,  Ryuichi Morishita ,  Yasufumi Kaneda ,  Hikaru Matsuda ,  Toshiki Yoshimine

IPC分类号： A61K48/00 ,  A61K9/127

CPC分类号： A61K9/0019 ,  A61K9/127 ,  A61K31/711 ,  A61K48/005 ,  A61K48/0075 ,  C07K14/4705 ,  C12N15/1131 ,  C12N15/115 ,  C12N15/86 ,  C12N2310/13 ,  C12N2310/315 ,  C12N2760/18811

摘要： The present invention provides introduction of NF-κB decoy oligodeoxynucleotide into rat cranial nerve through a carotid artery during global brain ischemia. Polymerase chain reaction demonstrated that one hour after global brain ischemia, transfected NF-κB decoy oligodeoxynucleotide effectively suppressed expression of tumor necrosis factor α, interleukin 1β and intracellular adhesion molecule 1 messenger RNAs. Terminal deoxynucleotidyl transferase-mediated deoxyuridine nick-end labeling staining and immunohistochemistry using microtubule-associated protein 2 demonstrated that transfected NF-κB decoy oligodeoxynucleotide significantly attenuated neuronal damage seven days after global brain ischemia. Therapeutic transfection of NF-κB decoy oligodeoxynucleotide during brain ischemia may be effective for attenuation of neuronal damage, suggesting a strategy for protecting the cerebrum from global ischemia.

摘要翻译： 本发明提供了在全脑缺血期间通过颈动脉将NF-κB诱饵寡脱氧核苷酸引入大鼠颅神经中。 聚合酶链反应表明，全球脑缺血后1小时，转染的NF-κB诱饵寡脱氧核苷酸有效抑制肿瘤坏死因子α，白细胞介素1β和细胞内粘附分子1信使RNA的表达。 末端脱氧核苷酸转移酶介导的脱氧尿苷切口标记染色和使用微管相关蛋白2的免疫组织化学证实，转染的NF-κB诱饵寡脱氧核苷酸在全脑缺血后7天显着减弱神经元损伤。 在脑缺血期间，NF-κB诱饵寡脱氧核苷酸的治疗转染对于减少神经元损伤可能是有效的，这表明保护大脑免受全身缺血的策略。

公开(公告)号：US20050239188A1

公开(公告)日：2005-10-27

申请号：US11126770

申请日：2005-05-10

申请人： Yasufumi Kaneda

发明人： Yasufumi Kaneda

IPC分类号： A61K48/00 ,  C12N7/04 ,  C12N15/87 ,  C12N15/88 ,  C12N15/86 ,  C12N7/00

CPC分类号： C12N7/00 ,  A61K48/00 ,  C12N15/87 ,  C12N15/88 ,  C12N2710/16663 ,  C12N2760/18823 ,  C12N2760/18861 ,  C12N2760/18863

摘要： A gene transfer vector is prepared by introducing an exogenous gene into an inactivated virus envelope, through a freezing and thawing treatment or mixing with a detergent. There are also provided a pharmaceutical composition for gene therapy containing this gene transfer vector, a kit containing this gene transfer vector, and a gene transfer method employing this gene transfer vector.

摘要翻译： 通过冷冻和解冻处理或与洗涤剂混合将外源基因导入灭活的病毒包膜中制备基因转移载体。 还提供了含有该基因转移载体的基因治疗药物组合物，含该基因转移载体的试剂盒和使用该基因转移载体的基因转移方法。

公开(公告)号：US06913923B2

公开(公告)日：2005-07-05

申请号：US09937839

申请日：2001-02-02

申请人： Yasufumi Kaneda

发明人： Yasufumi Kaneda

IPC分类号： A61K48/00 ,  C12N7/04 ,  C12N15/87 ,  C12N15/88 ,  C12N15/00 ,  C12N15/09 ,  C12N15/63 ,  C12N15/70 ,  C12N15/74

CPC分类号： C12N7/00 ,  A61K48/00 ,  C12N15/87 ,  C12N15/88 ,  C12N2710/16663 ,  C12N2760/18823 ,  C12N2760/18861 ,  C12N2760/18863

摘要： A gene transfer vector is prepared by introducing an exogenous gene into an inactivated virus envelope, through a freezing and thawing treatment or mixing with a detergent. There are also provided a pharmaceutical composition for gene therapy containing this gene transfer vector, a kit containing this gene transfer vector, and a gene transfer method employing this gene transfer vector.

公开(公告)号：US20120251510A1

公开(公告)日：2012-10-04

申请号：US13503329

申请日：2010-10-28

申请人： Katsuto Tamai ,  Yasufumi Kaneda ,  Takehiko Yamazaki ,  Takenao Chino ,  Kotaro Saga ,  Mayumi Endo

发明人： Katsuto Tamai ,  Yasufumi Kaneda ,  Takehiko Yamazaki ,  Takenao Chino ,  Kotaro Saga ,  Mayumi Endo

IPC分类号： A61K38/17 ,  C12N5/10 ,  C12N15/85 ,  C12N5/0775 ,  A61K31/7088 ,  A61P25/00 ,  A61P19/00 ,  A61P17/00 ,  C07K14/47 ,  A61K35/12

CPC分类号： A61K35/28 ,  A61K48/00 ,  C07K14/47

摘要： It was revealed that the intravenous administration of HMGB-1 and S100A8 promoted the healing of skin ulcer by recruiting bone marrow-derived cells to the site of skin ulcer. Furthermore, when HMGB-1 was intravenously administered to cerebral infarction model mice after creation of cerebral infarction, bone marrow-derived cells expressing nerve cell markers were detected in their brain. A marked cerebral infarct-reducing effect was observed in mice intravenously administered with HMGB-1 as compared to the control. The post-cerebral infarction survival rate was increased in the intravenous HMGB-1 administration group. The involvement of bone marrow pluripotent stem cells in the process of bone fracture healing was assessed using mice, and the result demonstrated that bone marrow-derived cells distant from the damaged site migrated to the bone fracture site to repair the damaged tissue.

摘要翻译： 据透露，HMGB-1和S100A8的静脉内给药通过将骨髓来源的细胞募集到皮肤溃疡部位来促进皮肤溃疡愈合。 此外，在脑梗死后，将HMGB-1静脉内施用于脑梗死模型小鼠时，在脑中检测到表达神经细胞标志物的骨髓来源的细胞。 与对照相比，静脉内施用HMGB-1的小鼠中观察到显着的脑梗死减轻作用。 静脉注射HMGB-1给药组脑梗死后存活率升高。 使用小鼠评估骨髓多能干细胞参与骨折愈合过程，结果表明远离受损部位的骨髓来源的细胞迁移到骨折部位以修复受损组织。

公开(公告)号：US08093101B2

公开(公告)日：2012-01-10

申请号：US11598816

申请日：2006-11-14

申请人： Kaoru Sakinada ,  Takumi Kooriike ,  Shunichi Aikawa ,  Osamu Kawachi ,  Yasufumi Kaneda

发明人： Kaoru Sakinada ,  Takumi Kooriike ,  Shunichi Aikawa ,  Osamu Kawachi ,  Yasufumi Kaneda

IPC分类号： H01L21/00

CPC分类号： B23K1/0016 ,  H01L23/10 ,  H01L24/97 ,  H01L2224/0554 ,  H01L2224/05568 ,  H01L2224/05573 ,  H01L2224/16225 ,  H01L2224/73253 ,  H01L2224/97 ,  H01L2924/00014 ,  H01L2924/01079 ,  H03H9/059 ,  H03H9/1078 ,  H03H9/1085 ,  H01L2224/81 ,  H01L2224/05599 ,  H01L2224/0555 ,  H01L2224/0556

摘要： There is provided a method of fabricating an electronic device including flip-chip mounting a device chip on a substrate, and supplying solder between adjacent device chips by supplying the solder on the device chips and applying heat and pressure on the solder, and a contact angle of the device chip and the solder is greater than 90° with the solder melted.

摘要翻译： 提供了一种制造电子器件的方法，该电子器件包括将器件芯片安装在衬底上的倒装芯片，以及通过在器件芯片上提供焊料并在焊料上施加热和压力来在相邻器件芯片之间提供焊料，以及接触角 的焊料熔化时，器件芯片和焊料大于90°。

公开(公告)号：US08049583B2

公开(公告)日：2011-11-01

申请号：US12397719

申请日：2009-03-04

申请人： Kouta Ohkubo ,  Yasufumi Kaneda

发明人： Kouta Ohkubo ,  Yasufumi Kaneda

IPC分类号： H03H9/64 ,  H03H9/25

CPC分类号： H03H9/02637 ,  H03H9/0057 ,  H03H9/6473 ,  H03H9/6476

摘要： An acoustic wave filter includes a piezoelectric substrate, an IDT (interdigital transducer) formed on the piezoelectric substrate, and reflectors located at both sides of the IDT and composed of electrode fingers, at least one of the electrode fingers of at least one of the reflectors including at least one gap within a propagation path of an acoustic wave.

摘要翻译： 声波滤波器包括压电基片，形成在压电基片上的IDT（叉指式换能器）和位于IDT两侧并由电极指构成的反射器，至少一个反射器的电极指中的至少一个 包括在声波的传播路径内的至少一个间隙。

公开(公告)号：US20110223148A1

公开(公告)日：2011-09-15

申请号：US13119148

申请日：2009-09-16

申请人： Yasufumi Kaneda ,  Yoshifumi Kawaguchi ,  Toshimitsu Itai

发明人： Yasufumi Kaneda ,  Yoshifumi Kawaguchi ,  Toshimitsu Itai

IPC分类号： A61K38/47 ,  A61P35/00

CPC分类号： C07K14/005 ,  A61K9/0019 ,  A61K9/19 ,  A61K31/76 ,  A61K35/768 ,  A61K45/06 ,  C12N2760/18833 ,  A61K2300/00

摘要： Provided are a novel therapeutic agent and therapeutic method for prostatic cancers. More specifically, a prostatic cancer therapeutic/prophylactic agent having a viral envelope vector, particularly a Sendai viral envelope vector, as an active ingredient, the therapeutic/prophylactic agent which is an apoptosis induction promoter, the therapeutic/prophylactic agent used for prostatic cancers whose androgen susceptibility has been partially or completely reduced, and a melanoma therapeutic/prophylactic agent containing a Sendai viral envelope vector as the only active ingredient, and the like are provided.

摘要翻译： 提供前列腺癌的新型治疗剂和治疗方法。 更具体地，具有病毒包膜载体，特别是仙台病毒包膜载体作为活性成分的前列腺癌治疗/预防剂，作为凋亡诱导启动子的治疗/预防剂，用于前列腺癌的治疗/预防剂，其用于前列腺癌 已经部分地或完全地降低了雄激素敏感性，并且提供了含有仙台病毒包膜载体作为唯一活性成分的黑素瘤治疗/预防剂等。

公开(公告)号：US20110104803A1

公开(公告)日：2011-05-05

申请号：US12990086

申请日：2009-04-30

申请人： Katsuto Tamai ,  Takehiko Yamazaki ,  Takenao Chino ,  Yasufumi Kaneda

发明人： Katsuto Tamai ,  Takehiko Yamazaki ,  Takenao Chino ,  Yasufumi Kaneda

IPC分类号： C12N5/071 ,  C12N5/077

CPC分类号： A61K35/28 ,  A61K2035/124 ,  C12N5/0663

摘要： Biologically low invasive vessels are filled with biological factors that have the activity of mobilizing specific functional cells in the body. The vessels are indwelled in the body. After specific functional cells are mobilized into the vessels, the vessels are removed from the body to collect functional cell populations mobilized to the vessels. Alternatively, the cells are directly collected from the vessels indwelled in the body.

摘要翻译： 生物低侵入血管充满生物因子，其具有在体内调动特定功能细胞的活性。 血管留在体内。 在将特定功能细胞移入血管后，将血管从体内移出以收集动员到血管的功能性细胞群。 或者，细胞直接从留在体内的血管中收集。

公开(公告)号：US20110097309A1

公开(公告)日：2011-04-28

申请号：US12990060

申请日：2009-04-30

申请人： Katsuto Tamai ,  Takehiko Yamazaki ,  Takenao Chino ,  Yasufumi Kaneda

发明人： Katsuto Tamai ,  Takehiko Yamazaki ,  Takenao Chino ,  Yasufumi Kaneda

IPC分类号： A61K35/12 ,  C12N5/02 ,  C12N15/63 ,  A61K38/00 ,  A61K31/7088 ,  C07K14/00 ,  A61P43/00

CPC分类号： A61K31/7088 ,  A01K2227/105 ,  A01K2267/0393 ,  A61K38/1709 ,  C07K14/47 ,  C12N15/8509

摘要： The present inventors assessed the possibility that bone marrow-derived cells are mobilized to the grafted skin from nonskin tissues and contribute to skin tissue regeneration during the engraftment of grafted skin on biological tissues. As a result, the present inventors for the first time in the world demonstrated that:(1) a large number of bone marrow-derived cells are mobilized to grafted skin;(2) mobilized bone marrow-derived cells differentiate into any of dermal fibroblasts, adipocytes, muscle cells, vascular endothelial cells, and epidermal keratinocytes in grafted skin, and thus mobilized bone marrow-derived cells include bone marrow-derived mesenchymal stem cells;(3) S100A8 and S100A9 released from necrotic tissues of grafted skin are responsible for mobilizing bone marrow-derived mesenchymal stem cells to the grafted skin from peripheral blood; and(4) purified S100A8 and S100A9 promote the migration of mesenchymal stem cells isolated/cultured from the bone marrow.

摘要翻译： 本发明人评估了骨髓来源的细胞从非组织组织移植到嫁接皮肤并有助于在生物组织移植移植皮肤期间皮肤组织再生的可能性。 结果，本发明人在世界上第一次证明：（1）大量骨髓来源的细胞被移动到移植皮肤; （2）动员的骨髓来源的细胞分化成皮肤成纤维细胞，脂肪细胞，肌肉细胞，血管内皮细胞和表皮角化细胞在移植皮肤中的任何一种，从而动员骨髓来源的细胞包括骨髓间充质干细胞; （3）从移植皮肤坏死组织中释放的S100A8和S100A9负责将骨髓间充质干细胞从外周血移植到移植皮肤; 和（4）纯化的S100A8和S100A9促进从骨髓​​分离/培养的间充质干细胞的迁移。