公开(公告)号：US20130212718A1

公开(公告)日：2013-08-15

申请号：US13852379

申请日：2013-03-28

Applicant: Malcolm James FRASER ,  Randy LEWIS ,  Don JARVIS ,  Kimberly THOMPSON ,  Joseph HULL ,  Yun-Gen MIAO ,  Florence TEULE ,  Bonghee SOHN ,  Youngsoo KIM

Inventor： Malcolm James FRASER ,  Randy LEWIS ,  Don JARVIS ,  Kimberly THOMPSON ,  Joseph HULL ,  Yun-Gen MIAO ,  Florence TEULE ,  Bonghee SOHN ,  Youngsoo KIM

IPC: C07K14/435

CPC classification number: C07K14/43586 ,  A01K67/0333 ,  A01K67/04 ,  A01K2217/052 ,  A01K2227/706 ,  A01K2267/01 ,  A01K2267/02 ,  A61K38/00 ,  C07K14/43518 ,  C07K2319/00

Abstract: Transgenic silkworms comprising at least one nucleic acid encoding a chimeric silk polypeptide comprising one or more spider silk elasticity and strength motifs are disclosed. Expression cassettes comprising nucleic acids encoding a variety of chimeric spider silk polypeptides (Spider 2, Spider 4, Spider 6, Spider 8) are also disclosed. A piggyBac vector system is used to incorporate nucleic acids encoding chimeric spider silk polypeptides into the mutant silkworms to generate stable transgenic silkworms. Chimeric silk fibers having improved tensile strength and elasticity characteristics compared to native silkworm silk fibers are also provided. The transgenic silkworms greatly facilitate the commercial production of chimeric silk fibers suitable for use in a wide variety of medical and industrial applications.

Abstract translation: 公开了包含编码包含一种或多种蜘蛛丝弹性和强度基序的嵌合丝多肽的至少一种核酸的转基因蚕。 还公开了包含编码多种嵌合蜘蛛丝多肽（Spider 2，Spider 4，Spider 6，Spider 8）的核酸的表达盒。 使用piggyBac载体系统将编码嵌合蜘蛛丝多肽的核酸掺入突变家蚕中以产生稳定的转基因蚕。 还提供了与天然蚕丝纤维相比具有改善的拉伸强度和弹性特征的嵌合丝纤维。 转基因家蚕大大促进了适用于各种医疗和工业应用的嵌合蚕丝纤维的商业生产。

公开(公告)号：US20130202569A1

公开(公告)日：2013-08-08

申请号：US13474120

申请日：2012-05-17

Applicant: David Lee Ayares

Inventor： David Lee Ayares

IPC: A61K38/17 ,  C12N15/85

CPC classification number: A61K38/1774 ,  A01K2217/05 ,  A01K2227/108 ,  A01K2267/02 ,  A01K2267/025 ,  C07K14/70521 ,  C07K2319/30 ,  C12N15/85 ,  C12N15/8509 ,  C12N2830/008

Abstract: The present invention provides ungulates, including pigs, expressing CTLA4-Ig, as well as tissue, organs, cells and cell lines derived from such animals. Such animals, tissues, organs and cells can be used in research and medical therapy, including xenotransplanation. In addition, methods are provided to prepare organs, tissues and cells expressing the CTLA4-Ig for use in xenotransplantation, and nucleic acid constructs and vectors useful therein.

公开(公告)号：US20130067606A1

公开(公告)日：2013-03-14

申请号：US13602927

申请日：2012-09-04

Applicant: Timothy James DORAN ,  Robert John MOORE ,  John William LOWENTHAL ,  Craig Allen SMITH ,  Andrew Henrik SINCLAIR

Inventor： Timothy James DORAN ,  Robert John MOORE ,  John William LOWENTHAL ,  Craig Allen SMITH ,  Andrew Henrik SINCLAIR

IPC: A01K67/027 ,  C12N15/113 ,  C12N5/10 ,  C12N1/21 ,  C12N1/15 ,  A61K31/713 ,  C12N15/63

CPC classification number: C12N15/8509 ,  A01K2217/058 ,  A01K2227/30 ,  A01K2267/02 ,  C12N15/111 ,  C12N15/113 ,  C12N15/1136 ,  C12N2310/14 ,  C12N2320/32

Abstract: The present invention relates to methods of modulating traits, particularly production traits, in avians such as chickens. In particular, the invention relates to the in ovo delivery of a dsRNA molecule, especially siRNAs, to modify production traits in commercially important birds.

Abstract translation: 本发明涉及在禽类如鸡中调节特性，特别是生产性状的方法。 特别地，本发明涉及dsRNA分子，特别是siRNAs的ovo递送，以改变商业上重要的鸟类的生产性状。

公开(公告)号：US08383880B2

公开(公告)日：2013-02-26

申请号：US12705509

申请日：2010-02-12

Applicant: Jen-Leih Wu ,  Shao-Yang Hu ,  Guor Mour Her

Inventor： Jen-Leih Wu ,  Shao-Yang Hu ,  Guor Mour Her

IPC: A01K67/027 ,  C12N15/00

CPC classification number: A01K67/0275 ,  A01K2217/052 ,  A01K2217/206 ,  A01K2227/40 ,  A01K2267/02 ,  C07K14/461 ,  C07K2319/60 ,  C12N9/0028 ,  C12N15/8509 ,  C12N2800/90

Abstract: A method of inducing male and/or female infertility in a genetically modified (GM) fish is disclosed. Also disclosed is a method of generating an infertile GM fish with a phenotype and/or genotype of interest. The method involves generation of a GM fish whose genome comprises a foreign transgene operably linked to a fish gonad-specific promoter selected from the group consisting of an ovary-specific promoter and a testis-specific promoter. The transgene comprises a suicide gene selected from the group consisting of a reductase and a photosensitizer, in which the reductase is operably linked to a reporter gene. Infertility of the GM fish is induced if the GM fish expressing the reductase is treated with an effective amount of a reductase-activated cytotoxic prodrug or if the GM fish expressing the photosensitizer is treated with light irradiation.

Abstract translation: 公开了在转基因鱼中诱导雄性和/或雌性不孕症的方法。 还公开了产生具有感兴趣的表型和/或基因型的不育GM鱼的方法。 该方法涉及产生GM基因组鱼，其基因组包含可操作地连接于选自卵巢特异性启动子和睾丸特异性启动子的鱼性腺特异性启动子的外源转基因。 转基因包含选自还原酶和光敏剂的自杀基因，其中还原酶可操作地连接到报告基因。 如果用有效量的还原酶激活的细胞毒性前药处理表达还原酶的GM鱼，或者如果用光照射处理表达光敏剂的GM鱼，则诱导GM鱼的不育。

公开(公告)号：US20130004472A1

公开(公告)日：2013-01-03

申请号：US13597069

申请日：2012-08-28

Applicant: Herman Pieter Spaink ,  Guido Everard Elisabeth Johannes Maria Van Den Thillart ,  Denhi Schnabel Peraza

Inventor： Herman Pieter Spaink ,  Guido Everard Elisabeth Johannes Maria Van Den Thillart ,  Denhi Schnabel Peraza

IPC: A61K35/12 ,  C12N5/10 ,  A61P5/06 ,  C12N5/07

CPC classification number: A61K38/24 ,  A01K67/0271 ,  A01K2207/12 ,  A01K2227/40 ,  A01K2267/02 ,  C07K14/59 ,  C07K2319/43

Abstract: Methods and/or hormone-producing cells for improving the development and/or maturation of eggs and/or sperm in fish using hormone administration. The methods may comprise providing the fish with cells producing the hormone. Preferred hormones are fertility hormones such as luteinizing hormone (LH), follicle-stimulating hormone (FSH) or chorionic gonadotropin (CG) or a functional part, derivative and/or analogue thereof.

Abstract translation: 使用激素给药改善鱼和/或精子在鱼中的发育和/或成熟的方法和/或激素产生细胞。 所述方法可以包括为鱼提供产生激素的细胞。 优选的激素是诸如黄体生成激素（LH），促卵泡激素（FSH）或绒毛膜促性腺激素（CG）或其功能部分，衍生物和/或类似物的生育激素。

公开(公告)号：US20120331576A1

公开(公告)日：2012-12-27

申请号：US13511980

申请日：2010-12-10

Applicant: Abraham Brass ,  Stephen Elledge

Inventor： Abraham Brass ,  Stephen Elledge

IPC: C12N5/10 ,  C12Q1/68 ,  C12Q1/70 ,  A01K67/027 ,  A61P31/04 ,  A61K9/127 ,  A61K38/17 ,  C12N7/00 ,  A61P31/12

CPC classification number: C12N7/00 ,  A01K2217/052 ,  A01K2217/075 ,  A01K2267/02 ,  A01K2267/0337 ,  A61K38/17 ,  A61K39/00 ,  A61K39/155 ,  C07K14/705 ,  C12N2740/13051 ,  C12N2760/10051 ,  C12N2760/16151 ,  C12N2760/20251 ,  C12N2770/24051 ,  C12N2770/24151 ,  C12Q1/18

Abstract: The use of interferon induced transmembrane protein 1, 2, or 3 (IFITM1, 2, or 3) as a viral restriction factor, and methods of using the same to produce virus, transgenic animals expressing exogenous IFITM1, 2, or 3, and methods of treating or inhibiting viral infections by targeting a gene identified herein

Abstract translation: 使用干扰素诱导的跨膜蛋白1,2或3（IFITM1,2或3）作为病毒限制因子，以及使用其来产生病毒的方法，表达外源性IFITM1,2或3的转基因动物以及方法 通过靶向本文鉴定的基因来治疗或抑制病毒感染

公开(公告)号：US20120317666A1

公开(公告)日：2012-12-13

申请号：US13561679

申请日：2012-07-30

Applicant: Alan Blake ,  Richard Crockett ,  Aidas Nasevicius

Inventor： Alan Blake ,  Richard Crockett ,  Aidas Nasevicius

IPC: A01K67/027

CPC classification number: A01K67/0275 ,  A01K2217/052 ,  A01K2267/02

Abstract: The present invention relates to transgenic blue ornamental fish, as well as methods of making such fish by in vitro fertilization techniques. Also disclosed are methods of establishing a population of such transgenic fish and methods of providing them to the ornamental fish industry for the purpose of marketing.

Abstract translation: 本发明涉及转基因蓝色观赏鱼，以及通过体外受精技术制备这种鱼的方法。 还公开了建立这样的转基因鱼种群的方法以及将其提供给观赏鱼业以销售为目的的方法。

公开(公告)号：US08318675B2

公开(公告)日：2012-11-27

申请号：US12736048

申请日：2009-03-05

Applicant: Frank Van Breusegem ,  Jan Staelens ,  Marnik Vuylsteke

Inventor： Frank Van Breusegem ,  Jan Staelens ,  Marnik Vuylsteke

IPC: A61K38/17 ,  C07K14/435

CPC classification number: A01K67/0338 ,  A01K2207/05 ,  A01K2227/70 ,  A01K2267/02 ,  A61K38/00 ,  C07K14/43509

Abstract: The invention relates to a crustacean androgenic gland hormone. More specifically, described are an androgenic gland hormone and associated peptides derived from Penaeus monodon, and their use to influence the sex ratio in prawn and shrimp cultures, and to set up monosex cultures. It is also related to the use of the hormone and associated peptides, associated gene or fragments thereof in sex determination.

Abstract translation: 本发明涉及甲壳动物激素腺体激素。 更具体地，描述了源自对虾（Monica）的雄激素激素和相关肽，以及它们用于影响虾和虾培养物中的性别比例，以及建立单体培养物。 它也与激素和相关肽，相关基因或其片段在性别测定中的应用有关。

公开(公告)号：US20120282226A1

公开(公告)日：2012-11-08

申请号：US13334194

申请日：2011-12-22

Applicant: David Ayares ,  Paul Rohricht

Inventor： David Ayares ,  Paul Rohricht

IPC: A61K35/32 ,  C12N5/071 ,  C12N5/077 ,  A61P17/00 ,  A61K38/39 ,  A61K35/36 ,  A61P19/02 ,  A61P19/08 ,  A01K67/027 ,  C07K14/78

CPC classification number: A61L27/3683 ,  A01K67/0276 ,  A01K2217/075 ,  A01K2227/108 ,  A01K2267/02 ,  A01K2267/025 ,  A61K35/12 ,  A61L27/3604 ,  A61L27/3629 ,  A61L27/3641 ,  A61L27/3695 ,  A61L2430/34 ,  C12N9/1051 ,  C12N15/8509

Abstract: The present invention provides tissues derived from animals, which lack any expression of functional alpha 1,3 galactosyltransferase (alpha-1,3-GT). Such tissues can be used in the field of xenotransplantation, such as orthopedic reconstruction and repair, skin repair and internal tissue repair or as medical devices.

Abstract translation: 本发明提供了来自动物的组织，其缺乏功能性α1,3半乳糖基转移酶（α-1,3-GT）的任何表达。 这样的组织可用于异种移植领域，例如矫形重建和修复，皮肤修复和内部组织修复或作为医疗装置。

公开(公告)号：US20120278910A1

公开(公告)日：2012-11-01

申请号：US13520056

申请日：2010-12-21

Applicant: Curie Ahn ,  Byeong Chun Lee ,  Jong Ik Hwang ,  Jae Seok Yang ,  Goo Jang ,  Bum Rae Cho ,  Ok Jae Koo ,  Jung Taek Kang ,  Dae Kee Kwon

Inventor： Curie Ahn ,  Byeong Chun Lee ,  Jong Ik Hwang ,  Jae Seok Yang ,  Goo Jang ,  Bum Rae Cho ,  Ok Jae Koo ,  Jung Taek Kang ,  Dae Kee Kwon

IPC: A01K67/027 ,  C12N5/071

CPC classification number: C07K14/7151 ,  A01K67/0275 ,  A01K2207/15 ,  A01K2217/052 ,  A01K2217/056 ,  A01K2217/15 ,  A01K2227/108 ,  A01K2267/02 ,  A01K2267/025 ,  C07K2319/30 ,  C12N9/0083 ,  C12N15/8509 ,  C12N2710/20051

Abstract: The present invention relates to a method for producing a transgenic pig in which immune rejection response is inhibited, and in which human HO-1 genes and TNFR1-Fe fusion genes are simultaneously expressed. The present invention also relates to a transgenic pig for organ transplantation, which is produced by the method, and in which immune rejection response is inhibited. The present invention also relates to a somatic-cell-donating cell strain for producing the transgenic pig, and to a method for producing organs, from the transgenic pig, in which the immune rejection response is inhibited.

Abstract translation: 本发明涉及抑制免疫排斥反应的转基因猪的制备方法，其中同时表达人HO-1基因和TNFR1-Fe融合基因。 本发明还涉及用于器官移植的转基因猪，其通过该方法产生，并且其中免疫排斥反应被抑制。 本发明还涉及用于产生转基因猪的体细胞供体细胞株以及从其中免疫排斥反应被抑制的转基因猪生产器官的方法。