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    Microorganisms and methods for the biosynthesis of fumarate, malate, and acrylate
    54.
    发明授权
    Microorganisms and methods for the biosynthesis of fumarate, malate, and acrylate 有权

    公开(公告)号：US09689006B2

    公开(公告)日：2017-06-27

    申请号：US14716767

    申请日：2015-05-19

    Applicant: Genomatica, Inc.

    Inventor： Mark J. Burk ,  Anthony P. Burgard ,  Priti Pharkya

    IPC: C12P7/40 ,  C12P7/46 ,  C12N9/88

    CPC classification number: C12P7/46 ,  C12N9/88 ,  C12P7/40

    Abstract: A non-naturally occurring eukaryotic or prokaryotic organism includes one or more gene disruptions occurring in genes encoding enzymes imparting increased fumarate, malate or acrylate production in the organism when the gene disruption reduces an activity of the enzyme. The one or more gene disruptions confers increased production of acrylate onto the organism. Organisms that produce acrylate have an acrylate pathway that at least one exogenous nucleic acid encoding an acrylate pathway enzyme expressed in a sufficient amount to produce acrylate, the acrylate pathway comprising a decarboxylase. Methods of producing fumarate, malate or acrylate include culturing these organisms.

    Semi-synthetic terephthalic acid via microorganisms that produce muconic acid
    57.
    发明授权
    Semi-synthetic terephthalic acid via microorganisms that produce muconic acid 有权
    半合成对苯二甲酸通过产生粘酸的微生物

    公开(公告)号：US09562241B2

    公开(公告)日：2017-02-07

    申请号：US14308292

    申请日：2014-06-18

    Applicant: GENOMATICA, INC.

    Inventor： Mark J. Burk ,  Robin E. Osterhout ,  Jun Sun

    IPC: C12N1/20 ,  C12P7/44

    CPC classification number: C12P7/44 ,  C12N9/88 ,  C12Y402/01002

    Abstract: The invention provides a non-naturally occurring microbial organism having a muconate pathway having at least one exogenous nucleic acid encoding a muconate pathway enzyme expressed in a sufficient amount to produce muconate. The muconate pathway including an enzyme selected from the group consisting of a beta-ketothiolase, a beta-ketoadipyl-CoA hydrolase, a beta-ketoadipyl-CoA transferase, a beta-ketoadipyl-CoA ligase, a 2-fumarylacetate reductase, a 2-fumarylacetate dehydrogenase, a trans-3-hydroxy-4-hexendioate dehydratase, a 2-fumarylacetate aminotransferase, a 2-fumarylacetate aminating oxidoreductase, a trans-3-amino-4-hexenoate deaminase, a beta-ketoadipate enol-lactone hydrolase, a muconolactone isomerase, a muconate cycloisomerase, a beta-ketoadipyl-CoA dehydrogenase, a 3-hydroxyadipyl-CoA dehydratase, a 2,3-dehydroadipyl-CoA transferase, a 2,3-dehydroadipyl-CoA hydrolase, a 2,3-dehydroadipyl-CoA ligase, a muconate reductase, a 2-maleylacetate reductase, a 2-maleylacetate dehydrogenase, a cis-3-hydroxy-4-hexendioate dehydratase, a 2-maleylacetate aminoatransferase, a 2-maleylacetate aminating oxidoreductase, a cis-3-amino-4-hexendioate deaminase, and a muconate cis/trans isomerase. Other muconate pathway enzymes also are provided. Additionally provided are methods of producing muconate.

    Abstract translation: 本发明提供了一种非天然存在的微生物，其具有一种具有至少一种外源核酸的粘蛋白途径的微生物生物，所述外源核酸编码以足够量表达的粘蛋白途径酶以产生粘蛋白。 所述粘蛋白途径包括选自β-酮硫解酶，β-酮己二酰辅酶A水解酶，β-酮己二酰辅酶A转移酶，β-酮己二酰辅酶A连接酶，2-富马酰乙酸酯还原酶，2- 富马酰乙酸脱氢酶，反式-3-羟基-4-己二酸脱水酶，2-富马酸乙酯氨基转移酶，2-富马酸乙酯胺化氧化还原酶，反式-3-氨基-4-己烯酸脱氨酶，β-酮己二酸烯醇内酯水解酶， 粘蛋白内酯异构酶，粘蛋白环异构酶，β-酮己二酰辅酶A脱氢酶，3-羟基己二酰辅酶A脱水酶，2,3-脱氢阿迪普基-CoA转移酶，2,3-脱氢阿迪普基-CoA水解酶，2,3-脱氢阿迪普酯 - CoA连接酶，粘蛋白还原酶，2-马来酰乙酸酯还原酶，2-马来酰乙酸酯脱氢酶，顺式-3-羟基-4-己二酸脱水酶，2-马来酰乙酸氨基转移酶，2-马来酰乙酸胺胺化氧化还原酶，顺式-3-氨基 -4-十六酸脱氨酶和粘蛋白顺式/反式异构酶。 还提供了其它粘蛋白途径酶。 另外提供了生产粘蛋白的方法。

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