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73 条记录 (耗时 0.12 秒)

    Method of nucleic acid cassette assembly
    51.
    发明授权
    Method of nucleic acid cassette assembly 有权

    公开(公告)号：US10041060B2

    公开(公告)日：2018-08-07

    申请号：US11635355

    申请日：2006-12-06

    申请人： J. Craig Venter ,  Hamilton O. Smith ,  Clyde A. Hutchison, III ,  Daniel G. Gibson

    发明人： J. Craig Venter ,  Hamilton O. Smith ,  Clyde A. Hutchison, III ,  Daniel G. Gibson

    IPC分类号： C12Q1/68 ,  C12N15/10 ,  C12N15/66 ,  C07H21/02

    摘要： Methods are provided for constructing a synthetic genome, comprising generating and assembling nucleic acid cassettes comprising portions of the genome, wherein at least one of the nucleic acid cassettes is constructed from nucleic acid components that have been chemically synthesized, or from copies of the chemically synthesized nucleic acid components. In one embodiment, the entire synthetic genome is constructed from nucleic acid components that have been chemically synthesized, or from copies of the chemically synthesized nucleic acid components. Rational methods may be used to design the synthetic genome (e.g., to establish a minimal genome and/or to optimize the function of genes within a genome, such as by mutating or rearranging the order of the genes). Synthetic genomes of the invention may be introduced into vesicles (e.g., bacterial cells from which part or all of the resident genome has been removed, or synthetic vesicles) to generate synthetic cells. Synthetic genomes or synthetic cells may be used for a variety of purposes, including the generation of synthetic fuels, such as hydrogen or ethanol.

    Methods for in vitro joining and combinatorial assembly of nucleic acid molecules
    57.
    发明授权
    Methods for in vitro joining and combinatorial assembly of nucleic acid molecules 有权
    核酸分子的体外连接和组合装配方法

    公开(公告)号：US08968999B2

    公开(公告)日：2015-03-03

    申请号：US12371543

    申请日：2009-02-13

    申请人： Daniel G. Gibson ,  Hamilton O. Smith ,  Clyde A. Hutchison ,  Lei Young ,  J. Craig Venter

    发明人： Daniel G. Gibson ,  Hamilton O. Smith ,  Clyde A. Hutchison ,  Lei Young ,  J. Craig Venter

    IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07H21/02 ,  C12N15/66 ,  C12N15/10 ,  C12N15/64

    CPC分类号： C12P19/34 ,  C12N15/10 ,  C12N15/1027 ,  C12N15/64 ,  C12N15/66

    摘要： The present invention relates to methods of joining two or more double-stranded (ds) or single-stranded (ss) DNA molecules of interest in vitro, wherein the distal region of the first DNA molecule and the proximal region of the second DNA molecule of each pair share a region of sequence identity. The method allows the joining of a large number of DNA fragments, in a predetermined order and orientation, without the use of restriction enzymes. It can be used, e.g., to join synthetically produced sub-fragments of a gene or genome of interest. Kits for performing the method are also disclosed. The methods of joining DNA molecules may be used to generate combinatorial libraries useful to generate, for example, optimal protein expression through codon optimization, gene optimization, and pathway optimization.

    摘要翻译： 本发明涉及在体外连接两个或多个目的双链（ds）或单链（ss）DNA分子的方法，其中第一DNA分子的远端区域和第二DNA分子的近端区域 每对共享序列同一性区域。 该方法允许以预定顺序和取向连接大量DNA片段，而不使用限制酶。 其可以用于例如合成产生的感兴趣的基因或基因组的亚片段。 还公开了用于执行该方法的套件。 连接DNA分子的方法可用于产生组合文库，其可用于产生例如通过密码子优化，基因优化和途径优化的最佳蛋白质表达。

    METHODS FOR IN VITRO JOINING AND COMBINATORIAL ASSEMBLY OF NUCLEIC ACID MOLECULES
    59.
    发明申请
    METHODS FOR IN VITRO JOINING AND COMBINATORIAL ASSEMBLY OF NUCLEIC ACID MOLECULES 有权
    核酸分子的体外联合和组合组装方法

    公开(公告)号：US20100035768A1

    公开(公告)日：2010-02-11

    申请号：US12371543

    申请日：2009-02-13

    申请人： Daniel G. GIBSON ,  Hamilton O. SMITH ,  Clyde A. HUTCHISON ,  Lei YOUNG ,  J. Craig VENTER

    发明人： Daniel G. GIBSON ,  Hamilton O. SMITH ,  Clyde A. HUTCHISON ,  Lei YOUNG ,  J. Craig VENTER

    IPC分类号： C12P19/34 ,  C40B40/08 ,  C07H21/04

    CPC分类号： C12P19/34 ,  C12N15/10 ,  C12N15/1027 ,  C12N15/64 ,  C12N15/66

    摘要： The present invention relates to methods of joining two or more double-stranded (ds) or single-stranded (ss) DNA molecules of interest in vitro, wherein the distal region of the first DNA molecule and the proximal region of the second DNA molecule of each pair share a region of sequence identity. The method allows the joining of a large number of DNA fragments, in a predetermined order and orientation, without the use of restriction enzymes. It can be used, e.g., to join synthetically produced sub-fragments of a gene or genome of interest. Kits for performing the method are also disclosed. The methods of joining DNA molecules may be used to generate combinatorial libraries useful to generate, for example, optimal protein expression through codon optimization, gene optimization, and pathway optimization.

    摘要翻译： 本发明涉及在体外连接两个或多个目的双链（ds）或单链（ss）DNA分子的方法，其中第一DNA分子的远端区域和第二DNA分子的近端区域 每对共享序列同一性区域。 该方法允许以预定顺序和取向连接大量DNA片段，而不使用限制酶。 其可以用于例如合成产生的感兴趣的基因或基因组的亚片段。 还公开了用于执行该方法的套件。 连接DNA分子的方法可用于产生组合文库，其可用于产生例如通过密码子优化，基因优化和途径优化的最佳蛋白质表达。

    Common Cold Coronavirus T Cell Epitopes, Methods and Uses Thereof
    60.
    发明公开
    Common Cold Coronavirus T Cell Epitopes, Methods and Uses Thereof 审中-公开

    公开(公告)号：US20240294900A1

    公开(公告)日：2024-09-05

    申请号：US18403239

    申请日：2024-01-03

    申请人： La Jolla Institute for Immunology ,  J. Craig Venter Institute

    发明人： Alessandro Sette ,  Alba Grifoni ,  Alison Tarke ,  Yun Zhang ,  Richard Scheuerman

    IPC分类号： C12N15/10

    CPC分类号： C12N15/1037 ,  C12N15/1055

    摘要： The present invention includes compositions and methods for detecting the presence of: a coronavirus or an immune response relevant to a coronavirus infection including T cells responsive to one or more coronavirus peptides or proteins comprising, consisting of, or consisting essentially of: one or more amino acid sequences selected from those sequences set forth in Tables 1 or 2 (SEQ ID NOS: 1 to 166), or a subsequence, portion, homologue, variant or derivative thereof; a fusion protein comprising one or more amino acid sequences selected from those sequences set forth in Tables 1 or 2 (SEQ ID NOS: 1 to 166); a pool of 2 or more peptides selected from the amino acid sequences set forth in Tables 1 or 2 (SEQ ID NOS: 1 to 166); or a polynucleotide that encodes one or more peptides or proteins, comprising, consisting of, or consisting essentially of an amino acid sequence selected from those sequences set forth in Tables 1 or 2 (SEQ ID NOS: 1 to 166), or a subsequence, portion, homologue, variant or derivative thereof. The invention further provides vaccines, diagnostics, therapies, and kits, comprising such proteins or peptides.