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105 条记录 (耗时 0.03 秒)

    Fluorescent resonance energy transfer probes
    53.
    发明申请
    Fluorescent resonance energy transfer probes 审中-公开
    荧光共振能量转移探针

    公开(公告)号：US20050042618A1

    公开(公告)日：2005-02-24

    申请号：US10635171

    申请日：2003-08-06

    申请人： Dieter Heindl ,  Hans-Peter Josel ,  Gregor Sagner ,  Ingrid Bechler

    发明人： Dieter Heindl ,  Hans-Peter Josel ,  Gregor Sagner ,  Ingrid Bechler

    IPC分类号： G01N33/53 ,  C12N15/09 ,  C12Q1/68 ,  G01N33/542 ,  G01N33/566 ,  G01N33/58 ,  C07H21/04

    CPC分类号： C12Q1/6818

    摘要： Compositions, reaction mixtures, and kits, as well as methods for their use, comprising a pair of FRET hybridization probes hybridizing adjacently to a target nucleic acid sequence, each hybridization probe comprising (i) a nucleotide sequence entity which is substantially complementary to the sequence of the target nucleic acid, (ii) a fluorescent entity, being either a FRET donor entity or a FRET acceptor entity, and (iii) a spacer entity connecting the nucleotide sequence entity and the fluorescent entity. The intensity of fluorescence emission from the FRET donor entity and from the FRET acceptor entity is not substantially affected by quenching activity of nucleotide residues present in the sequence of the target nucleic acid or in the nucleotide sequence entities of the hybridization probes.

    摘要翻译： 组合物，反应混合物和试剂盒及其使用方法包括与靶核酸序列相邻杂交的一对FRET杂交探针，每个杂交探针包含（i）与该序列基本互补的核苷酸序列实体 的靶核酸，（ii）作为FRET供体实体或FRET受体实体的荧光实体，和（iii）连接核苷酸序列实体和荧光实体的间隔实体。 来自FRET供体实体和FRET受体实体的荧光发射的强度基本上不受存在于靶核酸序列或杂交探针的核苷酸序列实体中的核苷酸残基的猝灭活性的影响。

    MINIATURIZED, HIGH-THROUGHPUT NUCLEIC ACID ANALYSIS
    56.
    发明申请
    MINIATURIZED, HIGH-THROUGHPUT NUCLEIC ACID ANALYSIS 有权
    微型化，高通量核酸分析

    公开(公告)号：US20130190191A1

    公开(公告)日：2013-07-25

    申请号：US13746812

    申请日：2013-01-22

    申请人： Thomas Froehlich ,  Dieter Heindl ,  Angelika Roesler

    发明人： Thomas Froehlich ,  Dieter Heindl ,  Angelika Roesler

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6874 ,  C12Q1/6834 ,  C12Q1/6846 ,  C12Q2563/149 ,  C12Q2525/197 ,  C12Q2523/319 ,  C12Q2565/537 ,  C12Q2547/101 ,  C12Q2537/143

    摘要： The present invention is directed to method for analyzing multiple nucleic acid molecules of interest comprising in the steps of (i) providing a plurality of beads, characterized in that each bead comprises at least two sequence specific amplification primers, further characterized in that at least one of the primers is bound to the bead via a cleavable linker, (ii) capturing the nucleic acid molecules of interest from a sample, (iii) clonally isolating the plurality of beads, (iv) cleaving the at least one primer, (v) clonally amplifying the nucleic acid thereby creating multiple amplification products, and (vi) analyzing the amplification products.

    摘要翻译： 本发明涉及用于分析感兴趣的多个核酸分子的方法，包括以下步骤：（i）提供多个珠粒，其特征在于每个珠粒包含至少两个序列特异性扩增引物，其特征还在于至少一个 的引物通过可切割的接头与珠结合，（ii）从样品中捕获感兴趣的核酸分子，（iii）克隆分离多个珠，（iv）切割至少一个引物，（v） 克隆扩增核酸从而产生多个扩增产物，和（vi）分析扩增产物。

    Nucleotide with an alpha-phosphate mimetic
    59.
    发明授权
    Nucleotide with an alpha-phosphate mimetic 失效
    具有α-磷酸酯模拟物的核苷酸

    公开(公告)号：US08153779B2

    公开(公告)日：2012-04-10

    申请号：US12580445

    申请日：2009-10-16

    申请人： Dieter Heindl

    发明人： Dieter Heindl

    IPC分类号： C07H19/10 ,  C07H19/20

    CPC分类号： C07H19/10 ,  C07H19/20 ,  C07H21/00

    摘要： Described are modified mononucleotides and processes for their production. The nucleotides have a structure in which B is a purine or pyrimidine, S is a sugar unit, Y is an OH, a monophosphate, or a diphosphate, and Acc is an electron acceptor selected from the group consisting of (a) —CN and (b) —SO2—R′ in which R′ contains one amino-substituted alkyl or one optionally substituted aryl.

    摘要翻译： 描述的是修饰的单核苷酸及其生产方法。 核苷酸具有其中B是嘌呤或嘧啶，S是糖单元，Y是OH，单磷酸酯或二磷酸酯的结构，并且Acc是选自（a）-CN和 （b）-SO 2 -R'，其中R'含有一个氨基取代的烷基或一个任选取代的芳基。