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177 条记录 (耗时 0.037 秒)

    Glycoprotein synthesis
    51.
    发明授权
    Glycoprotein synthesis 失效
    糖蛋白合成

    公开(公告)号：US07560535B2

    公开(公告)日：2009-07-14

    申请号：US11520849

    申请日：2006-09-12

    申请人： Peter G. Schultz ,  Lei Wang ,  Zhiwen Zhang

    发明人： Peter G. Schultz ,  Lei Wang ,  Zhiwen Zhang

    IPC分类号： C07K14/00 ,  A61K38/00

    CPC分类号： C12P21/005

    摘要： Methods for making glycoproteins, both in vitro and in vivo, are provided. One method involves incorporating an unnatural amino acid into a protein and attaching one or more saccharide moieties to the unnatural amino acid. Another method involves incorporating an unnatural amino acid that includes a saccharide moiety into a protein. Proteins made by both methods can be further modified with additional sugars.

    摘要翻译： 提供在体外和体内制备糖蛋白的方法。 一种方法包括将非天然氨基酸掺入蛋白质并将一个或多个糖部分连接到非天然氨基酸上。 另一种方法包括将包含糖部分的非天然氨基酸掺入蛋白质中。 通过两种方法制备的蛋白质可以用额外的糖进一步修饰。

    Glycoprotein synthesis
    52.
    发明授权
    Glycoprotein synthesis 失效

    公开(公告)号：US07262040B2

    公开(公告)日：2007-08-28

    申请号：US11093797

    申请日：2005-03-29

    申请人： Peter G. Schultz ,  Lei Wang ,  Zhiwen Zhang

    发明人： Peter G. Schultz ,  Lei Wang ,  Zhiwen Zhang

    IPC分类号： C12N9/10 ,  C12P19/00

    CPC分类号： C12P21/005

    摘要： Methods for making glycoproteins, both in vitro and in vivo, are provided. One method involves incorporating an unnatural amino acid into a protein and attaching one or more saccharide moieties to the unnatural amino acid. Another method involves incorporating an unnatural amino acid that includes a saccharide moiety into a protein. Proteins made by both methods can be further modified with additional sugars.

    Glycoprotein synthesis
    53.
    发明授权
    Glycoprotein synthesis 失效
    糖蛋白合成

    公开(公告)号：US07238510B2

    公开(公告)日：2007-07-03

    申请号：US11014402

    申请日：2004-12-14

    申请人： Peter G. Schultz ,  Lei Wang ,  Zhiwen Zhang

    发明人： Peter G. Schultz ,  Lei Wang ,  Zhiwen Zhang

    IPC分类号： C12N9/10 ,  C12N5/00 ,  C07K7/00

    CPC分类号： C12P21/005

    摘要： Methods for making glycoproteins, both in vitro and in vivo, are provided. One method involves incorporating an unnatural amino acid into a protein and attaching one or more saccharide moieties to the unnatural amino acid. Another method involves incorporating an unnatural amino acid that includes a saccharide moiety into a protein. Proteins made by both methods can be further modified with additional sugars.

    摘要翻译： 提供在体外和体内制备糖蛋白的方法。 一种方法包括将非天然氨基酸掺入蛋白质并将一个或多个糖部分连接到非天然氨基酸上。 另一种方法包括将包含糖部分的非天然氨基酸掺入蛋白质中。 通过两种方法制备的蛋白质可以用额外的糖进一步修饰。

    Site-Specific Incorporation of Redox Active Amino Acids into Proteins
    57.
    发明申请
    Site-Specific Incorporation of Redox Active Amino Acids into Proteins 审中-公开
    将氧化还原活性氨基酸与位点特异性结合到蛋白质中

    公开(公告)号：US20120100570A1

    公开(公告)日：2012-04-26

    申请号：US13344436

    申请日：2012-01-05

    申请人： Lital Alfonta ,  Peter G. Schultz ,  Zhiwen Zhang

    发明人： Lital Alfonta ,  Peter G. Schultz ,  Zhiwen Zhang

    IPC分类号： C12Q1/25 ,  C12N9/00

    CPC分类号： C12N9/93 ,  C12P21/02

    摘要： Compositions and methods of producing components of protein biosynthetic machinery that include orthogonal tRNAs, orthogonal aminoacyl-tRNA synthetases, and orthogonal pairs of tRNAs/synthetases, which incorporate redox active amino acids into proteins are provided. Methods for identifying these orthogonal pairs are also provided along with methods of producing proteins with redox active amino acids using these orthogonal pairs.

    摘要翻译： 提供了将氧化还原活性氨基酸掺入蛋白质的蛋白质生物合成机理组分的组成和方法，其中包括正交tRNA，正交氨酰-tRNA合成酶和tRNA /合成酶正交对。 还提供了用于鉴定这些正交对的方法以及使用这些正交对产生具有氧化还原活性氨基酸的蛋白质的方法。

    Site-specific incorporation of redox active amino acids into proteins
    58.
    发明授权
    Site-specific incorporation of redox active amino acids into proteins 有权
    将氧化还原活性氨基酸的位点特异性掺入蛋白质

    公开(公告)号：US08114654B2

    公开(公告)日：2012-02-14

    申请号：US12806751

    申请日：2010-08-20

    申请人： Lital Alfonta ,  Peter G. Schultz ,  Zhiwen Zhang

    发明人： Lital Alfonta ,  Peter G. Schultz ,  Zhiwen Zhang

    IPC分类号： C12Q1/68 ,  C12N9/00 ,  C12N1/20

    CPC分类号： C12N9/93 ,  C12P21/02

    摘要： Compositions and methods of producing components of protein biosynthetic machinery that include orthogonal tRNAs, orthogonal aminoacyl-tRNA synthetases, and orthogonal pairs of tRNAs/synthetases, which incorporate redox active amino acids into proteins are provided. Methods for identifying these orthogonal pairs are also provided along with methods of producing proteins with redox active amino acids using these orthogonal pairs.

    摘要翻译： 提供了将氧化还原活性氨基酸掺入蛋白质的蛋白质生物合成机理组分的组成和方法，其中包括正交tRNA，正交氨酰-tRNA合成酶和tRNA /合成酶正交对。 还提供了用于鉴定这些正交对的方法以及使用这些正交对产生具有氧化还原活性氨基酸的蛋白质的方法。

    Genetically programmed expression of selectively sulfated proteins in eubacteria
    59.
    发明授权
    Genetically programmed expression of selectively sulfated proteins in eubacteria 有权
    细菌中选择性硫酸化蛋白质的遗传程序表达

    公开(公告)号：US08114627B2

    公开(公告)日：2012-02-14

    申请号：US11903499

    申请日：2007-09-20

    申请人： Chang C. Liu ,  Peter G. Schultz

    发明人： Chang C. Liu ,  Peter G. Schultz

    IPC分类号： C12N1/20 ,  C12N9/00 ,  C12N9/10

    CPC分类号： C12N9/93 ,  C07K14/815 ,  C12P21/02

    摘要： The invention relates to orthogonal pairs of tRNAs and aminoacyl-tRNA synthetases that can incorporate the unnatural amino acid sulfotyrosine into proteins produced in eubacterial host cells such as E. coli. The invention provides, for example but not limited to, novel orthogonal aminoacyl-tRNA synthetases, polynucleotides encoding the novel synthetase molecules, methods for identifying and making the novel synthetases, methods for producing proteins containing the unnatural amino acid sulfotyrosine and translation systems.

    摘要翻译： 本发明涉及tRNA和氨酰-tRNA合成酶的正交对，其可以将非天然氨基酸磺基酪氨酸掺入到在细菌宿主细胞如大肠杆菌中产生的蛋白质中。 本发明提供了例如但不限于新型正交氨酰-tRNA合成酶，编码新合成酶分子的多核苷酸，用于鉴定和制备新合成酶的方法，用于产生含有非天然氨基酸磺酰酪氨酸的蛋白质的翻译方法和翻译系统。