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    Array of nucleic acid probes on biological chips for diagnosis of HIV and methods of using the same
    52.
    发明授权
    Array of nucleic acid probes on biological chips for diagnosis of HIV and methods of using the same 失效
    用于诊断HIV的生物芯片上的核酸探针阵列及其使用方法

    公开(公告)号：US5861242A

    公开(公告)日：1999-01-19

    申请号：US781550

    申请日：1997-01-09

    申请人： Mark Chee ,  Thomas R. Gingeras ,  Stephen P. A. Fodor ,  Earl A. Hubble ,  MacDonald S. Morris

    发明人： Mark Chee ,  Thomas R. Gingeras ,  Stephen P. A. Fodor ,  Earl A. Hubble ,  MacDonald S. Morris

    IPC分类号： B01J19/00 ,  C07B61/00 ,  C07H21/00 ,  C40B40/06 ,  C40B60/14 ,  C12Q1/68

    CPC分类号： B82Y30/00 ,  B01J19/0046 ,  C07H21/00 ,  C12Q1/703 ,  B01J2219/00432 ,  B01J2219/00529 ,  B01J2219/00596 ,  B01J2219/00608 ,  B01J2219/00626 ,  B01J2219/00659 ,  B01J2219/00689 ,  B01J2219/00711 ,  B01J2219/00722 ,  C07B2200/11 ,  C12Q1/6837 ,  C40B40/06 ,  C40B60/14 ,  Y10S435/81

    摘要： The invention provides an array of oligonucleotide probes immobilized on a solid support for analysis of a target sequence from a human immunodeficiency virus. The array comprises at least four sets of oligonucleotide probes 9 to 21 nucleotides in length. A first probe set has a probe corresponding to each nucleotide in a reference sequence from a human immunodeficiency virus. A probe is related to its corresponding nucleotide by being exactly complementary to a subsequence of the reference sequence that includes the corresponding nucleotide. Thus, each probe has a position, designated an interrogation position, that is occupied by a complementary nucleotide to the corresponding nucleotide. The three additional probe sets each have a corresponding probe for each probe in the first probe set. Thus, for each nucleotide in the reference sequence, there are four corresponding probes, one from each of the probe sets. The three corresponding probes in the three additional probe sets are identical to the corresponding probe from the first probe or a subsequence thereof that includes the interrogation position, except that the interrogation position is occupied by a different nucleotide in each of the four corresponding probes.

    摘要翻译： 本发明提供了固定在固体支持物上用于分析人免疫缺陷病毒靶序列的寡核苷酸探针阵列。 该阵列包含至少四组长度为9至21个核苷酸的寡核苷酸探针。 第一探针组具有对应于来自人类免疫缺陷病毒的参考序列中的每个核苷酸的探针。 探针通过与包括相应核苷酸的参考序列的亚序列完全互补而与其对应的核苷酸相关。 因此，每个探针具有被指定为与相应核苷酸的互补核苷酸占据的询问位置的位置。 三个附加的探针组各自具有在第一探针组中的每个探针的相应探针。 因此，对于参考序列中的每个核苷酸，存在四个对应的探针，每个探针组中的一个。 三个附加探针组中的三个相应的探针与来自第一探针的相应探针或其包含询问位置的亚序列相同，除了询问位置被四个相应探针中的每一个中的不同核苷酸占据。

    METHODS OF USING AN ARRAY OF POOLED PROBES IN GENETIC ANALYSIS
    57.
    发明申请
    METHODS OF USING AN ARRAY OF POOLED PROBES IN GENETIC ANALYSIS 审中-公开
    在遗传分析中使用沉积探针阵列的方法

    公开(公告)号：US20100041569A1

    公开(公告)日：2010-02-18

    申请号：US11876920

    申请日：2007-10-23

    申请人： Erik Gentalen ,  Mark Chee

    发明人： Erik Gentalen ,  Mark Chee

    IPC分类号： C40B40/06

    CPC分类号： C12Q1/6837 ,  C12Q1/6827 ,  C12Q1/6874 ,  C12Q2565/543 ,  C12Q2565/513

    摘要： The invention provides arrays of polynucleotide probes having at least one pooled position. A typical array comprises a support having at least three discrete regions. A first region bears a pool of polynucleotide probes comprising first and second probes. A second region bears the first probe without the second probe and a third region bears the second probe without the first probe. A target nucleic acid having segments complementary to both the first and second probes shows stronger normalized binding to the first region than to the aggregate of binding to the second and third regions due to cooperative binding of pooled probes in the first region. The invention provide methods of using such arrays for e.g., linkage analysis, sequence analysis, and expression monitoring.

    摘要翻译： 本发明提供具有至少一个合并位置的多核苷酸探针阵列。 典型的阵列包括具有至少三个离散区域的支撑件。 第一区域具有包含第一和第二探针的多核苷酸探针池。 第二区域承载第一探针而没有第二探针，第三区域承载第二探针而没有第一探针。 具有与第一和第二探针两者互补的区段的靶核酸由于在第一区域中的合并探针的协同结合而显示比第一区域更强的标准化结合，而不是与第二和第三区域结合的聚集体。 本发明提供了使用这种阵列进行例如连锁分析，序列分析和表达监测的方法。

    Methods for mixing fluid in a rotating container
    59.
    发明授权
    Methods for mixing fluid in a rotating container 有权
    将流体混合在旋转容器中的方法

    公开(公告)号：US07125161B2

    公开(公告)日：2006-10-24

    申请号：US10727877

    申请日：2003-12-03

    申请人： James L. Winkler ,  Mark Chee ,  David Lockhart

    发明人： James L. Winkler ,  Mark Chee ,  David Lockhart

    IPC分类号： B01F11/00

    CPC分类号： B01J19/28 ,  B01F9/0021 ,  B01J2219/00488 ,  B01J2219/00529 ,  B01J2219/00596 ,  B01J2219/00608 ,  B01J2219/00659 ,  B01J2219/00722 ,  C40B40/06 ,  C40B60/14 ,  Y10S435/809

    摘要： The invention provides exemplary devices, systems and methods for mixing of fluids, such as biological fluids. One exemplary device comprises is particularly adapted for holding at least one cartridge having a chamber containing a biological fluid, with the chamber having a generally planar face. The device comprises a rotatable body having a rotational axis. The rotatable body includes at least one mounting element which is adapted to mount the rotatable body such that the face of the chamber is generally perpendicular to the rotational axis.

    摘要翻译： 本发明提供了用于混合流体例如生物流体的示例性装置，系统和方法。 一个示例性装置包括特别适用于保持具有包含生物流体的腔室的至少一个筒，该腔室具有大致平坦的面。 该装置包括具有旋转轴线的可转动体。 可旋转主体包括至少一个安装元件，该安装元件适于安装可旋转主体，使得腔室的表面大致垂直于旋转轴线。