公开(公告)号：US20240102004A1

公开(公告)日：2024-03-28

申请号：US18345958

申请日：2023-06-30

申请人： THE BROAD INSTITUTE, INC. ,  MASSACHUSETTS INSTITUTE OF TECHNOLOGY ,  CHILDREN'S MEDICAL CENTER CORPORATION

发明人： Daniel E. Bauer ,  Stuart H. Orkin ,  Neville Espi Sanjana ,  Ophir Shalem ,  Jason Wright ,  Feng Zhang

IPC分类号： C12N15/10

CPC分类号： C12N15/1079 ,  C12N15/102 ,  C12N15/1075 ,  C12N15/1082

摘要： The application relates to a deep scanning mutagenesis library to interrogate phenotypic changes in a population of cells comprising a plurality of CRISPR-Cas system guide RNAs targeting genomic sequences within at least one continuous genomic region, wherein the guide RNAs target at least 100 genomic sequences upstream of a PAM sequence for every 1000 base pairs within the continuous genomic region and methods for their use.

公开(公告)号：US20240084330A1

公开(公告)日：2024-03-14

申请号：US18272158

申请日：2022-01-28

申请人： THE BROAD INSTITUTE, INC. ,  MASSACHUSETTS INSTITUTE OF TECHNOLOGY

发明人： Feng Zhang ,  Michael Segel ,  Blake Lash

IPC分类号： C12N15/88 ,  C07K14/005 ,  C07K14/47 ,  C12N9/22 ,  C12N15/11 ,  C12N15/86

CPC分类号： C12N15/88 ,  C07K14/005 ,  C07K14/47 ,  C12N9/22 ,  C12N15/11 ,  C12N15/86 ,  C12N2310/20 ,  C12N2740/16023 ,  C12N2740/16043 ,  C12N2740/16045 ,  C12N2760/20222

摘要： Provided herein are compositions, systems, and methods for delivering cargo to a target cell. The compositions, systems, and methods comprise one or more polynucleotides encoding one or more LTR retroelement polypeptides for forming a delivery vesicle and one or more capture moieties for packaging a cargo within the delivery vesicle. The one or more LTR retroelement polypeptides for forming a delivery vesicle may comprise two or more of an LTR retroelement gag protein, a retroelement envelope protein, a an LTR retroelement reverse transcriptase, or a combination thereof. The LTR retroelement polypeptide alone, the LTR retroelement envelope protein alone, or both the LTR retroelement-derived polypeptide and LTR retroelement envelope protein may be endogenous.

公开(公告)号：US11898142B2

公开(公告)日：2024-02-13

申请号：US16645571

申请日：2018-09-07

申请人： THE BROAD INSTITUTE, INC. ,  MASSACHUSETTS INSTITUTE OF TECHNOLOGY ,  PRESIDENT AND FELLOWS OF HARVARD COLLEGE

发明人： Feng Zhang ,  Jonathan Gootenberg ,  Omar Abudayyeh

IPC分类号： C12N15/113 ,  C12N9/22 ,  G01N33/53 ,  B01L3/00

CPC分类号： C12N15/113 ,  B01L3/5085 ,  C12N9/22 ,  G01N33/5308 ,  B01L2300/123 ,  B01L2300/126 ,  C12N2310/20 ,  C12N2800/80

摘要： The embodiments disclosed herein utilized RNA targeting effectors to provide a robust CRISPR-based diagnostic with attomolar sensitivity. Embodiments disclosed herein can detect both DNA and RNA with comparable levels of sensitivity and can differentiate targets from non-targets based on single base pair differences. Moreover, the embodiments disclosed herein can be prepared in freeze-dried format for convenient distribution and point-of-care (POC) applications. Such embodiments are useful in multiple scenarios in human health including, for example, viral detection, bacterial strain typing, sensitive genotyping, and detection of disease-associated cell free DNA.

公开(公告)号：US20240043828A1

公开(公告)日：2024-02-08

申请号：US18030324

申请日：2021-10-05

申请人： THE BROAD INSTITUTE, INC. ,  MASSACHUSETTS INSTITUTE OF TECHNOLOGY

发明人： Feng Zhang ,  Alim Ladha

IPC分类号： C12N15/10 ,  C12N9/22 ,  C12N15/11 ,  C12N9/12 ,  C07K14/195

CPC分类号： C12N15/102 ,  C12N9/22 ,  C12N15/11 ,  C12N9/1252 ,  C12Y207/07007 ,  C07K14/195 ,  C12N2310/20

摘要： Described in several embodiments herein are compositions, systems, and methods for targeted gene modification, targeted insertion, perturbation of gene transcripts, and nucleic acid editing. Described in several embodiments are nucleic acid targeting systems that include components of CRISPR systems, VirD polypeptide(s), and DNA polymerase(s).

公开(公告)号：US20240026382A1

公开(公告)日：2024-01-25

申请号：US18470189

申请日：2023-09-19

申请人： THE BROAD INSTITUTE, INC. ,  MASSACHUSETTS INSTITUTE OF TECHNOLOGY

发明人： Feng Zhang ,  Soumya Kannan ,  Han Altae-Tran

IPC分类号： C12N15/90 ,  C12N9/22 ,  C12N15/10 ,  C12N9/78

CPC分类号： C12N15/90 ,  C12N9/22 ,  C12N15/102 ,  C12N9/78 ,  C12Y305/04005 ,  C12Y305/04004 ,  C12Y207/07049

摘要： Described herein are systems, methods, and compositions capable of targeting nucleic acids. Describe in certain exemplary embodiments herein are a class of small Cas proteins (Type II-D Cas proteins) and systems thereof. Also described in certain exemplary embodiments herein are methods of modifying target sequences using the class of small Cas proteins (Type II-D Cas proteins) and systems thereof described herein.

公开(公告)号：US11851702B2

公开(公告)日：2023-12-26

申请号：US16894664

申请日：2020-06-05

申请人： THE BROAD INSTITUTE, INC. ,  MASSACHUSETTS INSTITUTE OF TECHNOLOGY

发明人： Feng Zhang ,  Jonathan Gootenberg ,  Omar Abudayyeh ,  Julia Joung ,  Alim Ladha ,  Han Altae-Tran ,  Guilhem Faure

IPC分类号： C12Q1/68 ,  C12Q1/6844 ,  C12N9/22 ,  C12Q1/6806 ,  C12N9/12 ,  C12N9/78 ,  B01L3/00 ,  B01L7/00 ,  C12Q1/70

CPC分类号： C12Q1/6844 ,  B01L3/502 ,  B01L3/502715 ,  B01L7/52 ,  C12N9/1276 ,  C12N9/22 ,  C12N9/78 ,  C12Q1/6806 ,  C12Q1/701 ,  B01L2300/0816 ,  C12N2310/20 ,  C12Q2600/156 ,  C12Q2600/16 ,  C12Y207/07049 ,  G01N2333/165

摘要： Systems and methods for rapid diagnostics related to the use of isothermal amplification reagents for detection of microbial species, including coronavirus, and methods of use, are provided.

公开(公告)号：US20230392131A1

公开(公告)日：2023-12-07

申请号：US18032868

申请日：2021-10-22

申请人： THE BROAD INSTITUTE, INC. ,  MASSACHUSETTS INSTITUTE OF TECHNOLOGY

发明人： Feng Zhang ,  Han Altae-Tran ,  Soumya Kannan ,  Fatma Esra Demircioglu

IPC分类号： C12N9/22 ,  C12N15/10 ,  C12N15/63

CPC分类号： C12N9/22 ,  C12N15/102 ,  C12N15/63 ,  C12N2310/20

摘要： Systems, methods and compositions for targeting polynucleotides are detailed herein. In particular, engineered DNA-targeting systems comprising IscB polypeptides, novel IscB nucleases and reprogrammable targeting nucleic acid components and methods and application of use are provided.

公开(公告)号：US11773432B2

公开(公告)日：2023-10-03

申请号：US17554314

申请日：2021-12-17

申请人： The Broad Institute, Inc. ,  Massachusetts Institute of Technology ,  President and Fellows of Harvard College

发明人： Feng Zhang ,  Bernd Zetsche ,  Johnathan S. Gootenberg ,  Omar O. Abudayyeh ,  Ian Slaymaker

IPC分类号： C12Q1/6832 ,  C12N15/11 ,  C12N9/22 ,  C12N15/113 ,  C12Q1/6816

CPC分类号： C12Q1/6832 ,  C12N9/22 ,  C12N15/11 ,  C12N15/113 ,  C12Q1/6816 ,  C12N2310/20 ,  C12N2800/80 ,  C12Q1/6816 ,  C12Q2521/301

摘要： The invention provides for systems, methods, and compositions for targeting nucleic acids. In particular, the invention provides non-naturally occurring or engineered DNA-targeting systems comprising a novel DNA-targeting CRISPR effector protein and at least one targeting nucleic acid component like a guide RNA. Methods for making and using and uses of such systems, methods, and compositions and products from such methods and uses are also disclosed and claimed.

公开(公告)号：US11773412B2

公开(公告)日：2023-10-03

申请号：US17848563

申请日：2022-06-24

申请人： The Broad Institute, Inc. ,  Massachusetts Institute of Technology ,  President and Fellows of Harvard College ,  Rutgers, The State University of New Jersey ,  Skolkovo Institute of Science and Technology ,  The United States of America, as represented by the Secretary, Dept. of Health and Human Services

发明人： Konstantin Severinov ,  Feng Zhang ,  Yuri I. Wolf ,  Sergey Shmakov ,  Ekaterina Semenova ,  Leonid Minakhin ,  Kira S. Makarova ,  Eugene Koonin ,  Silvana Konermann ,  Julia Joung ,  Jonathan S. Gootenberg ,  Omar O. Abudayyeh ,  Eric S. Lander

IPC分类号： C12N15/90 ,  C12N15/10 ,  C12N15/63 ,  C12N9/22 ,  C12N15/11 ,  C12N15/82 ,  C12N15/85 ,  C12N15/113

CPC分类号： C12N15/907 ,  C12N9/22 ,  C12N15/102 ,  C12N15/111 ,  C12N15/113 ,  C12N15/63 ,  C12N15/8201 ,  C12N15/85 ,  C12N2310/111 ,  C12N2310/20 ,  C12N2800/22

摘要： The invention provides for systems, methods, and compositions for targeting nucleic acids. In particular, the invention provides non-naturally occurring or engineered RNA-targeting systems comprising a novel RNA-targeting CRISPR effector protein and at least one targeting nucleic acid component like a guide RNA.

公开(公告)号：US11739308B2

公开(公告)日：2023-08-29

申请号：US16493464

申请日：2018-03-15

申请人： The Broad Institute, Inc. ,  Massachusetts Institute of Technology

发明人： David Benjamin Turitz Cox ,  Neena Pyzocha ,  Feng Zhang

IPC分类号： C12N9/22 ,  C12N15/86

CPC分类号： C12N9/22 ,  C12N15/86 ,  C12N2310/20 ,  C12N2800/30

摘要： The invention provides for systems, methods, and compositions for targeting nucleic acids. In particular, the invention provides non-naturally occurring or engineered RNA-targeting systems comprising a novel RNA-targeting Cas13b effector protein and at least one targeting nucleic acid component like a guide RNA or crRNA.