公开(公告)号：US4021307A

公开(公告)日：1977-05-03

申请号：US584595

申请日：1975-06-06

申请人： Klaus H. Mosbach

发明人： Klaus H. Mosbach

IPC分类号： G01N25/20 ,  C12M1/34 ,  C12M1/40 ,  C12Q1/00 ,  G01N25/48 ,  G01N31/14

CPC分类号： G01N25/4873

摘要： A method and apparatus for measuring temperature changes generated by enzyme activity consists in conducting a matrixbound enzyme through a flowpath formed by a helical coil and sensing the temperature in the immediate vicinity of the flowpath.

摘要翻译： 用于测量由酶活性产生的温度变化的方法和装置在于通过由螺旋线圈形成的流动路径进行基质结合的酶，并感测在流动路径附近的温度。

公开(公告)号：US3029640A

公开(公告)日：1962-04-17

申请号：US83976459

申请日：1959-09-14

申请人： CUTLER HAMMER INC

发明人： SCHMIDT EDWIN X ,  SEVERSON PALMER T

IPC分类号： G01F3/30 ,  G01N25/48

CPC分类号： G01N25/4873 ,  G01F3/30

公开(公告)号：US11686697B2

公开(公告)日：2023-06-27

申请号：US17030675

申请日：2020-09-24

申请人： International Business Machines Corporation

发明人： Andrea Fasoli

IPC分类号： G01N27/12 ,  H01L29/24 ,  G01N27/14 ,  G01N25/18 ,  G01N25/48 ,  G01N25/20

CPC分类号： G01N27/125 ,  G01N25/18 ,  G01N25/482 ,  G01N25/4873 ,  G01N27/12 ,  G01N27/14 ,  H01L29/24 ,  G01N25/20 ,  G01N25/48 ,  G01N27/123

摘要： A method of identifying a gas is provided. The method includes providing a gas sensor device comprising at least two stacked metal oxide layers, wherein a change in conductance of the gas sensor device in a presence of a gas varies with a temperature of the stacked metal oxide layers. The method includes bringing the gas into proximity with the stacked metal oxide layers. The method also includes measuring the conductance of the gas sensor device when the gas is in proximity with the stacked layers at multiple temperatures to generate a temperature-conductance profile. The method also includes identifying a gas of interest based on the temperature-conductance profile.

公开(公告)号：US20190195701A1

公开(公告)日：2019-06-27

申请号：US16092614

申请日：2017-04-13

申请人： TECHNISCHE UNIVERSITEIT DELFT

发明人： Kourosh HONARMAND EBRAHIMI ,  Morten VAN SCHIE ,  Peter Leon HAGEDOORN ,  Wilfred Raymond HAGEN

IPC分类号： G01K17/00 ,  G01N25/48 ,  G01K3/08 ,  G01N21/05

CPC分类号： G01K17/006 ,  G01K3/08 ,  G01K19/00 ,  G01N21/05 ,  G01N25/482 ,  G01N25/4873 ,  G01N2021/0346

摘要： The invention provides a calibration method for calibrating a chip-based microfluidic calorimeter, wherein the chip-based microfluidic calorimeter comprises one or more thermopiles, wherein the calibration method uses the deprotonating reaction of a phosphate group, the method comprising: providing calibration liquids comprising (i) a buffer with a pH range of at least 7-9 and (ii) a first compound with a phosphate group which is protonated in a pH range of at least 3-6, and mixing these calibration liquids in the chip-based microfluidic calorimeter to provide a calibration liquid mixture whereby heat is generated, measuring the heat by the thermopiles and thereby providing a corresponding thermopile signal, and calibrating the chip-based microfluidic calorimeter by relating the thermopile signal to reference data of the deprotonating reaction.

公开(公告)号：US09915636B2

公开(公告)日：2018-03-13

申请号：US14784749

申请日：2014-04-15

申请人： Dräger Safety AG & Co. KGaA

发明人： Hans-Ullrich Hansmann ,  Philipp Rostalski

IPC分类号： G01N33/00 ,  G01N31/22 ,  G01N25/48 ,  G01N21/78 ,  G01N21/81

CPC分类号： G01N31/223 ,  G01N21/783 ,  G01N21/81 ,  G01N25/482 ,  G01N25/4873 ,  G01N31/222 ,  G01N33/0013 ,  G01N33/0036 ,  G01N2201/1211 ,  G01N2201/1214

摘要： A reaction carrier (14), a measuring device (12) and a measuring method measure a concentration of gaseous and/or aerosol components of a gas mixture. A flow channel (42), extends between two connecting elements (44) and defines a reaction chamber (46) with an optically detectable reaction material (48) that reacts a component of the gas mixture or with a reaction product of the component. The reaction carrier (14) includes a temperature-measuring element (88). The measuring device (12) includes a temperature-measuring element (90) which records a temperature of the measuring device (12) and/or of the reaction carrier (14), and a temperature-determining unit (92) which determines the temperature of the gas mixture as a function of the measurement result of the at least one temperature-measuring element (90). The measuring method includes determining a concentration of the component on the basis of an optically detectable reaction and the determined temperature of the gas mixture.

公开(公告)号：US20160363548A1

公开(公告)日：2016-12-15

申请号：US15192712

申请日：2016-06-24

申请人： Malvern Instruments Incorporated

发明人： Martin BROGA ,  Phillip PRICE ,  Stephen SMITH

IPC分类号： G01N25/48 ,  G01N35/00 ,  G01N35/10

CPC分类号： G01N25/4873 ,  B01L3/02 ,  G01K17/006 ,  G01N1/14 ,  G01N25/20 ,  G01N25/48 ,  G01N25/4813 ,  G01N25/4866 ,  G01N35/00584 ,  G01N35/0092 ,  G01N35/1002

摘要： Automated isothermal titration micro calorimetry (ITC) system comprising a micro calorimeter with a sample cell and a reference cell, the sample cell is accessible via a sample cell stem and the reference cell is accessible via a reference cell stem. The system further comprises an automatic pipette assembly comprising a syringe with a titration needle arranged to be inserted into the sample cell for supplying titrant, the pipette assembly comprises an activator for driving a plunger in the syringe, a pipette translation unit supporting the pipette assembly and being arranged to place pipette in position for titration, washing and filling operations, a wash station for the titrant needle, and a cell preparation unit arranged to perform operations for replacing the sample liquid in the sample cell when the pipette is placed in another position than the position for titration.

公开(公告)号：US09404876B2

公开(公告)日：2016-08-02

申请号：US14736905

申请日：2015-06-11

申请人： Malvern Instruments Incorporated

发明人： Martin Broga ,  Phillip Price ,  Stephen Smith

IPC分类号： G01N25/20 ,  B01L3/02 ,  G01N35/00 ,  G01N35/10 ,  G01N25/48 ,  G01K17/00 ,  G01N1/14

CPC分类号： G01N25/4873 ,  B01L3/02 ,  G01K17/006 ,  G01N1/14 ,  G01N25/20 ,  G01N25/48 ,  G01N25/4813 ,  G01N25/4866 ,  G01N35/00584 ,  G01N35/0092 ,  G01N35/1002

摘要： Automated isothermal titration micro calorimetry (ITC) system comprising a micro calorimeter with a sample cell and a reference cell, the sample cell is accessible via a sample cell stem and the reference cell is accessible via a reference cell stem. The system further comprises an automatic pipette assembly comprising a syringe with a titration needle arranged to be inserted into the sample cell for supplying titrant, the pipette assembly comprises an activator for driving a plunger in the syringe, a pipette translation unit supporting the pipette assembly and being arranged to place pipette in position for titration, washing and filling operations, a wash station for the titrant needle, and a cell preparation unit arranged to perform operations for replacing the sample liquid in the sample cell when the pipette is placed in another position than the position for titration.

公开(公告)号：US09228970B2

公开(公告)日：2016-01-05

申请号：US13991227

申请日：2011-11-25

申请人： Bart Van Grinsven ,  Ward De Ceuninck ,  Patrick Wagner ,  Luc Michiels

发明人： Bart Van Grinsven ,  Ward De Ceuninck ,  Patrick Wagner ,  Luc Michiels

IPC分类号： C12M1/34 ,  C12M3/00 ,  C12M1/00 ,  C12Q1/68 ,  C07H21/02 ,  C07H21/04 ,  G01N15/06 ,  G01N27/00 ,  G01N25/00 ,  G01N31/22 ,  G01N27/327 ,  G01N27/02 ,  B01L3/00 ,  G05B15/02

CPC分类号： G01N27/327 ,  B01L3/50273 ,  B01L3/502738 ,  B01L2300/023 ,  B01L2300/0636 ,  C12Q1/6825 ,  C12Q1/6827 ,  C12Q1/6832 ,  G01N25/4873 ,  G01N27/021 ,  G01N27/3275 ,  G05B15/02 ,  Y10T436/11 ,  Y10T436/143333 ,  C12Q2527/101 ,  C12Q2527/113 ,  C12Q2565/607

摘要： A method an system is disclosed for characterizing DNA and/or RNA duplexes. The biosensing device comprises a heating element using a power and being suitable for providing thermal denaturation of target DNA and/or RNA bioparticles, a sample holder adapted for receiving a biocompatible substrate having a functionalized surface which is coated with probe DNA and/or RNA whereto target DNA and/or RNA duplexes can be attached, the sample holder further being adapted for exposing the biocompatible substrate at one side to the heating element, a first temperature sensing element for sensing a temperature at the side where the biocompatible substrate can be exposed to the heating element and a second temperature sensing element for sensing a temperature at the side opposite thereto with respect to the biocompatible substrate. The device also comprises a processing means programmed for calculating at least one heat transfer resistivity value based on temperature values obtained with the first temperature sensing element and the second temperature sensing element and the power for the heating element, for deriving a characteristic of the target DNA and/or RNA bioparticles from said heat transfer resistivity value.

摘要翻译： 公开了用于表征DNA和/或RNA双链体的方法。 生物传感装置包括使用功率并适合于提供靶DNA和/或RNA生物颗粒的热变性的加热元件，适于接收生物相容性底物的样品保持器，其具有涂覆有探针DNA和/或RNA的官能化表面， 可以连接靶DNA和/或RNA双链体，样品保持器还适于将一侧的生物相容性基底暴露于加热元件，第一温度感测元件，用于感测生物相容性基底可以暴露于的一侧的温度 加热元件和第二温度感测元件，用于感测与生物相容性基底相对的一侧的温度。 该装置还包括一个处理装置，其被编程用于基于用第一温度感测元件和第二温度感测元件获得的温度值和加热元件的功率来计算至少一个传热电阻率值，用于导出靶DNA的特征 和/或RNA生物颗粒从所述传热电阻率值。

公开(公告)号：US09103782B2

公开(公告)日：2015-08-11

申请号：US12326300

申请日：2008-12-02

申请人： Martin Broga ,  Phillip Price ,  Stephen Smith

发明人： Martin Broga ,  Phillip Price ,  Stephen Smith

IPC分类号： G01N25/20 ,  B01L3/02 ,  G01N25/48 ,  G01K17/00 ,  G01N1/14

CPC分类号： G01N25/4873 ,  B01L3/02 ,  G01K17/006 ,  G01N1/14 ,  G01N25/20 ,  G01N25/48 ,  G01N25/4813 ,  G01N25/4866 ,  G01N35/00584 ,  G01N35/0092 ,  G01N35/1002

摘要： Automated isothermal titration micro calorimetry (ITC) system comprising a micro calorimeter with a sample cell and a reference cell, the sample cell is accessible via a sample cell stem and the reference cell is accessible via a reference cell stem. The system further comprises an automatic pipette assembly comprising a syringe with a titration needle arranged to be inserted into the sample cell for supplying titrant, the pipette assembly comprises an activator for driving a plunger in the syringe, a pipette translation unit supporting the pipette assembly and being arranged to place pipette in position for titration, washing and filling operations, a wash station for the titrant needle, and a cell preparation unit arranged to perform operations for replacing the sample liquid in the sample cell when the pipette is placed in another position than the position for titration.

摘要翻译： 自动等温滴定微量热法（ITC）系统，其包括具有样品池和参比细胞的微量热计，样品池可通过样品细胞茎进行，参考细胞可通过参考细胞茎进入。 所述系统还包括自动移液管组件，其包括具有滴定针的注射器，所述滴定针被布置成插入到所述样品池中用于供应滴定剂，所述移液管组件包括用于驱动所述注射器中的柱塞的活化剂，支持所述移液管组件的移液管平移单元， 布置成将移液管放置在适当位置用于滴定，洗涤和灌装操作，用于滴定针的洗涤台，以及细胞制备单元，其被配置为当移液管放置在另一位置时执行替换样品池中的样品液体的操作， 滴定的位置。

公开(公告)号：US20150219584A1

公开(公告)日：2015-08-06

申请号：US14567792

申请日：2014-12-11

申请人： IMEC ,  UNIVERSITEIT HASSELT

发明人： Bart Van Grinsven ,  Ward De Ceuninck ,  Patrick Wagner

IPC分类号： G01N27/327 ,  G05B15/02 ,  B01L3/00

CPC分类号： G01N27/327 ,  B01L3/50273 ,  B01L3/502738 ,  B01L2300/023 ,  B01L2300/0636 ,  C12Q1/6825 ,  C12Q1/6827 ,  C12Q1/6832 ,  G01N25/4873 ,  G01N27/021 ,  G01N27/3275 ,  G05B15/02 ,  Y10T436/11 ,  Y10T436/143333 ,  C12Q2527/101 ,  C12Q2527/113 ,  C12Q2565/607

摘要： A method an system is disclosed for the detection and/or allocation of at least one point mutation in target DNA and/or RNA duplexes. The method comprises obtaining a functionalized surface which is coated with probe DNA and/or RNA whereto target DNA and/or RNA duplexes are attached, contacting said functionalized surface to an electrolytic solution having a neutral pH in a flow cell and measuring a first impedance value within said electrolytic solution, and then adding a chemical to the electrolytic solution which is able to achieve denaturation of the target DNA and/or RNA. The method further comprises measuring a second impedance value within the flow cell after completion of the denaturation of the DNA and/or RNA target, and then obtaining a value representative for the impact of the chemical on the impedance of the electrolytic solution. The amount and/or allocation of point mutation(s) within the target DNA and/or RNA is then determined by calculating the denaturation-time constant based on the difference between the first and second impedance value and taking into account the impact of the chemical by third impedance value.

摘要翻译： 公开了用于检测和/或分配靶DNA和/或RNA双链体中的至少一个点突变的方法。 所述方法包括获得涂覆有靶DNA和/或RNA双链体的探针DNA和/或RNA的功能化表面，使所述功能化表面与流动池中具有中性pH的电解溶液接触并测量第一阻抗值 在所述电解液中，然后向能够实现靶DNA和/或RNA变性的电解液中加入化学物质。 该方法还包括在完成DNA和/或RNA靶变性后测量流动池内的第二阻抗值，然后获得代表该化学物质对电解溶液阻抗的影响的值。 然后通过基于第一和第二阻抗值之间的差异计算变性 - 时间常数并考虑到化学品的影响来确定靶DNA和/或RNA内的点突变的量和/或分配 通过第三阻抗值。