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    Mutant microorganism producing succinic acid simultaneously using sucrose and glycerol, and method for preparing succinic acid using same
    61.
    发明授权
    Mutant microorganism producing succinic acid simultaneously using sucrose and glycerol, and method for preparing succinic acid using same 有权
    使用蔗糖和甘油同时生产琥珀酸的突变体微生物，以及使用其制备琥珀酸的方法

    公开(公告)号：US08691516B2

    公开(公告)日：2014-04-08

    申请号：US13819339

    申请日：2011-08-30

    申请人： Sang Yup Lee ,  Jeong Wook Lee ,  Sol Choi ,  Jongho Yi

    发明人： Sang Yup Lee ,  Jeong Wook Lee ,  Sol Choi ,  Jongho Yi

    IPC分类号： G01N33/53 ,  C12N1/20

    CPC分类号： C12P7/46 ,  C12N1/20 ,  C12N15/52 ,  C12R1/01

    摘要： The present invention relates to a succinic acid-producing mutant microorganism that is able to utilize sucrose and glycerol simultaneously as carbon sources. More particularly, the present invention relates to a succinic acid-producing mutant microorganism that is able to utilize sucrose and glycerol simultaneously for succinic acid production, the mutant organism being obtained by relieving the mechanism of sucrose-mediated catabolite repression of glycerol in a succinic acid-producing microorganism. As described above, when the succinic acid-producing mutant microorganism is cultured, it utilizes sucrose and glycerol simultaneously so that succinic acid can be produced with high productivity in a maximum yield approaching the theoretical yield while the production of byproducts is minimized. In addition, according to the present invention, various reduced chemicals which have been produced in low yields in conventional methods can be more effectively produced.

    摘要翻译： 本发明涉及能够同时使用蔗糖和甘油作为碳源的产琥珀酸突变体微生物。 更具体地，本发明涉及能够同时使用蔗糖和甘油进行琥珀酸生产的产琥珀酸的突变体微生物，其通过减轻甘油在琥珀酸中的蔗糖介导的分解代谢物抑制的机制而获得 生产微生物。 如上所述，当培养产琥珀酸的突变体微生物时，它同时使用蔗糖和甘油，使得以最大产率接近理论产率的高生产率生产琥珀酸，同时副产物的产生最小化。 此外，根据本发明，可以更有效地制造在常规方法中以低收率生产的各种还原化学品。

    Method for screening essential metabolites in growth of microorganisms
    63.
    发明授权
    Method for screening essential metabolites in growth of microorganisms 有权
    筛选微生物生长中重要代谢物的方法

    公开(公告)号：US08494779B2

    公开(公告)日：2013-07-23

    申请号：US12519791

    申请日：2007-12-21

    申请人： Sang Yup Lee ,  Hawoong Jeong ,  Tae Yong Kim ,  Pan-Jun Kim ,  Kwang Ho Lee

    发明人： Sang Yup Lee ,  Hawoong Jeong ,  Tae Yong Kim ,  Pan-Jun Kim ,  Kwang Ho Lee

    IPC分类号： G06F19/10 ,  G06F19/12

    CPC分类号： C12Q1/18 ,  G01N33/5038

    摘要： The present invention disclosed is a method for screening metabolites essential for the growth of microorganism using metabolic flux analysis. More specifically, the present invention relates to the method for screening metabolites essential for the growth of microorganism, by selecting a target microorganism, constructing a metabolic network model of the selected microorganism, inactivating the consumption reaction of each of metabolites in the constructed metabolic network model, analyzing the metabolic flux of the metabolites to select metabolites essential for the growth of the microorganism, and confirming the selected metabolites using the utilization of each of the metabolites, defined as flux sum (Φ). According to the present invention, metabolites essential for the growth of microorganism, and genes involved in the essential metabolites, can be screened in a convenient manner, and drug-target genes against pathogenic microorganisms can be predicted by deleting genes associated with the metabolites screened according to the method.

    摘要翻译： 所公开的本发明是使用代谢通量分析来筛选微生物生长所必需的代谢物的方法。 更具体地说，本发明涉及通过选择靶微生物，构建所选择的微生物的代谢网络模型，在构建的代谢网络模型中灭活各代谢物的消费反应来筛选微生物生长所必需的代谢物的方法 分析代谢物的代谢通量以选择对于微生物生长至关重要的代谢物，并且使用定义为通量总和（Phi）的每种代谢物来确认所选择的代谢物。 根据本发明，可以以便利的方式筛选微生物生长所必需的代谢物和基本代谢物所涉及的基因，并且可以通过删除与代谢物相关的基因来预测针对病原微生物的药物靶基因 的方法。

    METHOD OF PREPARING PROTEIN HAVING HIGH CONTENT OF SPECIFIC AMINO ACID BY CO-EXPRESSION WITH TRNA OF SPECIFIC AMINO ACID
    64.
    发明申请
    METHOD OF PREPARING PROTEIN HAVING HIGH CONTENT OF SPECIFIC AMINO ACID BY CO-EXPRESSION WITH TRNA OF SPECIFIC AMINO ACID 审中-公开
    具有特异性氨基酸的高含量的蛋白质的制备方法与特异性氨基酸的TRNA通过共表达

    公开(公告)号：US20110244515A1

    公开(公告)日：2011-10-06

    申请号：US13120404

    申请日：2009-09-22

    申请人： Sang Yup Lee ,  Xiaoxia Xia ,  Zhi Gang Qian ,  Jong Hwan Baek

    发明人： Sang Yup Lee ,  Xiaoxia Xia ,  Zhi Gang Qian ,  Jong Hwan Baek

    IPC分类号： C12P21/00 ,  C12N1/21 ,  C12N15/63

    CPC分类号： C12N15/67 ,  C07K14/43518 ,  C07K14/43586

    摘要： The present invention relates to a method of increasing the expression of a target protein by co-expression of a gene encoding a target protein having a high content of a specific amino acid with a nucleotide sequence encoding the tRNA of the specific amino acid. According to the present invention, the expression of a protein having a high content of a specific amino acid can be remarkably increased by co-expression with the tRNA of the specific amino acid. Thus, the present invention is useful for increasing the productivity of a protein having a high content of a specific amino acid, such as a repetitive protein.

    摘要翻译： 本发明涉及通过将编码具有高特异性氨基酸含量的靶蛋白的基因与编码特定氨基酸的tRNA的核苷酸序列共表达来增加靶蛋白的表达的方法。 根据本发明，通过与特定氨基酸的tRNA共表达，可以显着提高具有高特异性氨基酸含量的蛋白质的表达。 因此，本发明对于提高具有高含量的特定氨基酸的蛋白质如重复蛋白质的生产率是有用的。

    Detection Method of Bio-Chemical Material Using Surface-Enhanced Raman Scattering
    65.
    发明申请
    Detection Method of Bio-Chemical Material Using Surface-Enhanced Raman Scattering 审中-公开
    使用表面增强拉曼散射的生物化学材料的检测方法

    公开(公告)号：US20110165586A1

    公开(公告)日：2011-07-07

    申请号：US12900248

    申请日：2010-10-07

    申请人： Bongsoo Kim ,  Taejoon Kang ,  Ilsun Yoon ,  Sang Yup Lee ,  Seung Min Yoo

    发明人： Bongsoo Kim ,  Taejoon Kang ,  Ilsun Yoon ,  Sang Yup Lee ,  Seung Min Yoo

    IPC分类号： G01N33/53 ,  G01N21/00 ,  G01N33/48

    CPC分类号： G01N21/658 ,  Y10T436/143333

    摘要： Provided is a detection method of a biochemical material using surface-enhanced Raman scattering in order to detect the existence of a biochemical material in a target subject or its content therein, more particularly a detection method of a biochemical material facilitating multiplex detection with high-sensitivity, high-reproducibility, high-reliability, and high-precision owing to multiple hot spots formed on the nanowire surface of a single crystal body by the bond of multiple nanoparticles which are physically separated from each other.

    摘要翻译： 提供了使用表面增强拉曼散射来生物化学材料的检测方法，以检测目标受试者中的生物化学物质的存在或其含量，更具体地说，一种促进多重检测的生化材料的检测方法，具有高灵敏度 由于通过物理上彼此分离的多个纳米颗粒的结合而在单晶体的纳米线表面上形成的多个热点，具有高再现性，高可靠性和高精度。

    ENHANCED ETHANOL AND BUTANOL PRODUCING MICROORGANISMS AND METHOD FOR PREPARING ETHANOL AND BUTANOL USING THE SAME
    66.
    发明申请
    ENHANCED ETHANOL AND BUTANOL PRODUCING MICROORGANISMS AND METHOD FOR PREPARING ETHANOL AND BUTANOL USING THE SAME 审中-公开
    生产微生物的增强的乙醇和丁醇和使用它们制备乙醇和丁醇的方法

    公开(公告)号：US20110027845A1

    公开(公告)日：2011-02-03

    申请号：US12809323

    申请日：2008-12-22

    申请人： Sang Yup Lee ,  Yu-Sin Jang ,  Jin Young Lee ,  Kwang Seop Jung ,  Jae Hyun Kim

    发明人： Sang Yup Lee ,  Yu-Sin Jang ,  Jin Young Lee ,  Kwang Seop Jung ,  Jae Hyun Kim

    IPC分类号： C12P7/16 ,  C12N1/00 ,  C12N1/21 ,  C12P7/06

    CPC分类号： C12P7/065 ,  C12N9/0006 ,  C12N9/13 ,  C12P7/16 ,  Y02E50/10 ,  Y02E50/17

    摘要： The present invention relates to a recombinant microorganism having an enhanced ability to produce ethanol and butanol and a method for preparing ethanol and butanol using the same, and more particularly to a recombinant microorganism having an enhanced ability to produce ethanol and butanol, into which a gene encoding CoA transferase and a gene encoding alcohol/aldehyde dehydrogenase are introduced, and to a method for preparing ethanol and butanol using the same. The recombinant microorganism according to the present invention, obtained by manipulating metabolic pathways of microorganisms, is capable of producing butanol and ethanol exclusively without producing any byproduct, and thus is useful as a microorganism producing industrial solvents and transportation fuel.

    摘要翻译： 本发明涉及具有提高乙醇和丁醇生产能力的重组微生物，以及使用该重组微生物制备乙醇和丁醇的方法，更具体地说，涉及生产乙醇和丁醇的能力增强的重组微生物，其中基因 引入编码CoA转移酶和编码醇/醛脱氢酶的基因，以及使用其制备乙醇和丁醇的方法。 根据本发明的通过操纵微生物代谢途径获得的重组微生物能够仅生产丁醇和乙醇而不产生任何副产物，因此可用作产生工业溶剂和运输燃料的微生物。