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    System for sustained microbial production of hydrogen gas in a bioreactor utilizing a circulation system
    82.
    发明申请
    System for sustained microbial production of hydrogen gas in a bioreactor utilizing a circulation system 审中-公开
    利用循环系统在生物反应器中持续微生物生产氢气的系统

    公开(公告)号：US20060281164A1

    公开(公告)日：2006-12-14

    申请号：US11450637

    申请日：2006-06-09

    申请人： Harry Diz ,  Mitchell Felder ,  Justin Felder

    发明人： Harry Diz ,  Mitchell Felder ,  Justin Felder

    IPC分类号： C12P3/00 ,  C12M3/00

    CPC分类号： C12P3/00 ,  C12M21/04 ,  C12M41/26 ,  C12M41/28 ,  C12M45/20 ,  C12M47/18 ,  Y02E50/343

    摘要： The present invention provides an apparatus for the production of hydrogen from microorganisms, wherein a bioreactor provides an environment conducive to the production of hydrogen from hydrogen producing microorganisms and restrictive to the production of methane from methanogens. The environment is adjusted to a pH conducive to the growth and metabolism of hydrogen producing microorganisms within the bioreactor, wherein the pH of the organic feed material is preferably between about 3.5 and 6.0 pH. The method further includes a circulation system to create directional Low within the bioreactor.

    摘要翻译： 本发明提供了一种用于从微生物生产氢气的装置，其中生物反应器提供有利于产生氢气的微生物产生氢气的环境，并限制从产甲烷菌生产甲烷。 将环境调节至有利于生物反应器内生产氢的微生物的生长和代谢的pH，其中有机进料的pH优选在约3.5至6.0 pH之间。 该方法还包括在生物反应器内产生定向低的循环系统。

    Method for manufacturing organic acid by high-efficiency continuous fermentation
    84.
    发明授权
    Method for manufacturing organic acid by high-efficiency continuous fermentation 失效
    通过高效连续发酵制造有机酸的方法

    公开(公告)号：US06596521B1

    公开(公告)日：2003-07-22

    申请号：US09529570

    申请日：2000-04-13

    申请人： Ho Nam Chang ,  Yong Keun Chang ,  Sun Hoon Kwon ,  Woo Gi Lee ,  Pyung Cheon Lee ,  Ik Keun Yoo ,  Seong Jin Lim

    发明人： Ho Nam Chang ,  Yong Keun Chang ,  Sun Hoon Kwon ,  Woo Gi Lee ,  Pyung Cheon Lee ,  Ik Keun Yoo ,  Seong Jin Lim

    IPC分类号： C12P740

    CPC分类号： C12P7/46 ,  C12M23/58 ,  C12M29/18 ,  C12M41/26 ,  C12P7/40 ,  C12P7/42 ,  C12P7/48

    摘要： The present invention provides a method for manufacturing organic acid by high-efficiency fermentation, which comprises the steps of continuous culture of organic acid bacteria and collection of organic acid produced from the culture employing a cell-recycle multiple-stage continuous fermentor with serially connected fermentors, each of which comprises a fermentor containing a ferment container, temperature controller, stirrer, and pH controller; pumps for efflux-circulation of media from the fermentor; and, cell separator for separation and circulation of media from the pumps. According to the present method, the high-concentration lactic acid of 90 g/L can be produced with the high productivity of 50 g/L/h, which can reduce the facility cost and production cost in the bulk manufacturing process. In addition, the present invention can be effectively applied to the production of other organic acids such as acetic acid, formic acid, citric acid, malic acid, maleic acid, fumaric acid, and succinic acid, which show the end-product inhibition.

    摘要翻译： 本发明提供了一种通过高效发酵制造有机酸的方法，其包括以下步骤：连续培养有机酸细菌和收集由使用连续发酵罐的细胞循环多级连续发酵罐的培养物产生的有机酸 ，其中每个包含含有发酵容器，温度控制器，搅拌器和pH控制器的发酵罐; 用于来自发酵罐的介质的外排循环的泵; 以及用于从泵分离和循环介质的细胞分离器。 根据本方法，可以以50g / L / h的高生产率生产90g / L的高浓度乳酸，这可以降低批量制造过程中的设备成本和生产成本。 此外，本发明可以有效地应用于生产其它有机酸如乙酸，甲酸，柠檬酸，苹果酸，马来酸，富马酸和琥珀酸，这些有机酸显示出最终产物的抑制作用。

    Method of control of Haematococcus spp, growth process
    86.
    发明授权
    Method of control of Haematococcus spp, growth process 失效
    抗生球菌的防治方法，生长过程

    公开(公告)号：US5882849A

    公开(公告)日：1999-03-16

    申请号：US673063

    申请日：1996-07-01

    申请人： Alexander B. P. Leonard ,  Mark E. Huntley ,  Pearn P. Niiler ,  Donald Redalje

    发明人： Alexander B. P. Leonard ,  Mark E. Huntley ,  Pearn P. Niiler ,  Donald Redalje

    IPC分类号： C12M1/00 ,  C12M1/107 ,  C12M1/42 ,  C12N1/12 ,  C12P23/00 ,  C12Q3/00

    CPC分类号： C12N1/12 ,  C12M21/02 ,  C12M23/36 ,  C12M41/00 ,  C12M41/06 ,  C12M41/12 ,  C12M41/26 ,  C12P23/00

    摘要： A control method for operating aqueous Haematococcus spp., such as H. pluvialis, microorganism growth processes is disclosed which can maintain viable growth conditions of this microorganism which have heretofore not been easily reproduced in commercially valuable quantities. The primary control parameters are the degree of turbulence in the aqueous growth medium and the scale of the apparatus relative to the scale of the turbulent eddies in vessels which are partially filled with the aqueous medium directly affect conditions which are required for optimum growth: light exposure, nutrient supply, sedimentation rate, bulk temperature, gas exchange rate and cell integrity. These control elements can be cast in terms of the Reynolds number (N.sub.re), pH, temperature, amount of impinging light, and NO.sub.2 concentration, depending upon the operative chlorophyll growth stages of Haematococcus spp. and its photoadaptive stages of producing astaxanthin.

    摘要翻译： 公开了一种用于操作水溶性红潮球菌属例如H.luluulisis的微生物生长方法的控制方法，其可保持迄今为止不易以商业上有价值重现的该微生物的生长生长条件。 主要控制参数是水性生长培养基中的湍流程度，并且装置的规模相对于部分填充有水介质的容器中的湍流涡流的比例直接影响最佳生长所需的条件：光照 营养供应，沉淀速率，体温，气体交换率和细胞完整性。 这些控制元素可以根据雷诺数（Nre），pH值，温度，入射光量和NO2浓度来进行投射，这取决于流感嗜血杆菌属的操作叶绿素生长阶段。 及其摄影适应期生产虾青素。

    Apparatus and method for anaerobic respirometry
    87.
    发明授权
    Apparatus and method for anaerobic respirometry 失效
    厌氧呼吸机的设备和方法

    公开(公告)号：US5811255A

    公开(公告)日：1998-09-22

    申请号：US530539

    申请日：1995-09-20

    申请人： Robert M. Hunter ,  Frank M. Stewart

    发明人： Robert M. Hunter ,  Frank M. Stewart

    IPC分类号： C12M1/34 ,  C12Q1/04 ,  C12Q1/02

    CPC分类号： C12M41/40 ,  C12M41/26 ,  C12M41/28 ,  C12M41/30 ,  C12M41/46 ,  C12Q1/04

    摘要： An apparatus and method for anaerobic and aerobic respirometry. The apparatus and method provide for automatically collecting and analyzing the data required to calibrate mathematical models for bioprocesses that involve anaerobic respiration, aerobic respiration and dehalogenation. Dissolved electron-acceptor concentrations and/or product concentrations and/or headspace pressures are automatically monitored during the progress of a biotransformation occurring in a batch reactor to produce a data set. The data set is analyzed to derive intrinsic kinetic parameters and stoichiometric coefficients. The cultures biocatalyzing the oxidation-reduction reactions of interest may be aerobic, denitrifying (e.g., nitrate-reducing), sulfate reducing and/or methanogenic. The models thus developed may be used for design of wastewater treatment or bioremediation processes.

    摘要翻译： 用于厌氧和有氧呼吸测量的装置和方法。 该装置和方法提供用于自动收集和分析校准用于涉及厌氧呼吸，有氧呼吸和脱卤的生物过程的数学模型所需的数据。 在间歇反应器中发生的生物转化过程中，自动监测溶解的电子受体浓度和/或产物浓度和/或顶空压力，以产生数据集。 分析数据集以得到固有动力学参数和化学计量系数。 生物催化感兴趣的氧化 - 还原反应的培养物可能是好氧，脱氮（例如硝酸盐还原），硫酸盐还原和/或产甲烷。 如此开发的模型可用于废水处理或生物修复过程的设计。

    Cell growing device for in vitro cell population expansion
    88.
    发明授权
    Cell growing device for in vitro cell population expansion 失效
    用于体外细胞群体扩增的细胞生长装置

    公开(公告)号：US5627070A

    公开(公告)日：1997-05-06

    申请号：US506173

    申请日：1995-07-26

    申请人： Micheal Gruenberg

    发明人： Micheal Gruenberg

    IPC分类号： C12M3/06 ,  C12M1/36

    CPC分类号： C12M29/16 ,  C12M23/58 ,  C12M25/10 ,  C12M41/26 ,  C12M41/34

    摘要： A cell growing device for in vitro cell population growth includes at least one hollow fiber cartridge having a plurality of capillaries at least one of which is selectively permeable. The flow of media out of a lumen of the cartridge is substantially blocked off thereby forcing media flowing into the lumen via an inflow opening to permeate across the capillaries of the cartridge and into the extracapillary space thereof.

    摘要翻译： 用于体外细胞群体生长的细胞培养装置包括至少一个具有多个毛细管的中空纤维筒，其中至少一个是选择性渗透的。 介质从药筒的内腔流出基本上被阻断，从而迫使通过流入开口流入内腔的介质渗透穿过药筒的毛细管并进入其毛细血管空间。

    Process and apparatus for removing ammonium from aqueous liquids
    89.
    发明授权
    Process and apparatus for removing ammonium from aqueous liquids 失效
    从水性液体中除去氨的方法和装置

    公开(公告)号：US5130026A

    公开(公告)日：1992-07-14

    申请号：US705655

    申请日：1991-05-24

    申请人： Manfred Biselli ,  Joerg Thoemmes ,  Christian Wandrey

    发明人： Manfred Biselli ,  Joerg Thoemmes ,  Christian Wandrey

    IPC分类号： B01D15/04 ,  B01D61/24 ,  B01J47/12 ,  C01C1/02 ,  C02F1/469 ,  C02F1/58 ,  C12M1/12 ,  C12M3/06

    CPC分类号： B01D61/243 ,  C12M29/04 ,  C12M41/26 ,  C12M47/10

    摘要： A process for the removal of ammonium from aqueous liquids, especially from fermentation broths, is disclosed. The process uses a cation exchanger membrane which separates a liquid on a donor side that is to be depleted of NH.sub.4.sup.+ from a solution on an acceptor side whose pH is higher than on the donor side. Because of the equilibriumNH.sub.4.sup.+ .revreaction.NH.sub.3 +H+there is on the acceptor side increased formation of NH.sub.3. This is continuously removed, in particular by reducing the pressure and/or passing through inert gas, or by selective transport through another microporous membrane into an acid protonation solution, so that a NH.sub.4.sup.+ concentration gradient is maintained across the membrane. A similar content, apart from the ammonium concentration, of concomitant cations on the donor and acceptor side and/or a high ratio of the solution volumes on the donor side and the acceptor side ensures that the solution on the donor side to be depleted of NH.sub.4.sup.+, does not lose concomitant ions. A suitable apparatus comprises a flow section which can be coupled into a bypass and has a cation exchanger limiting membrane which is simultaneously a limiting wall of an acceptor chamber. The acceptor chamber may contain devices for pH control and NH.sub.3 removal or these may be contained in a flow section.

    摘要翻译： 公开了从含水液体，特别是发酵液中除去铵的方法。 该方法使用阳离子交换膜，其将待排出NH 4 +的供体侧上的液体从pH高于供体侧的受体侧上的溶液分离。 由于平衡NH4 + - > < - NH3 + H +在受体侧上增加了NH3的形成。 这是连续去除的，特别是通过降低压力和/或通过惰性气体，或通过选择性地将另一个微孔膜输送到酸性质子溶液中，使得横跨该膜保持NH 4 +浓度梯度。 供体和受体侧上伴随的阳离子和/或供体侧和受体侧的溶液体积的高比例，除了铵浓度以外，类似的含量确保供体侧的溶液被耗尽NH4 + ，不会伴随离子丢失。 合适的装置包括流动部分，其可以耦合到旁路中并且具有同时是受体室的限制壁的阳离子交换器限制膜。 受体室可以包含用于pH控制和NH 3去除的装置，或者这些装置可以包含在流动部分中。