公开(公告)号：US20130252329A1

公开(公告)日：2013-09-26

申请号：US13909128

申请日：2013-06-04

申请人： Technion Research & Development Foundation Limited

发明人： Michal AMIT ,  Joseph Itskovitz-Eldor

IPC分类号： C12N5/0735

CPC分类号： C12N5/0606 ,  C12N5/0037 ,  C12N5/0043 ,  C12N5/0056 ,  C12N5/0602 ,  C12N5/0603 ,  C12N2500/90 ,  C12N2500/98 ,  C12N2501/115 ,  C12N2501/15 ,  C12N2501/23 ,  C12N2501/2306 ,  C12N2506/02 ,  C12N2533/52

摘要： Well-defined, xeno-free culture media which comprise a TGF-beta isoform or the chimera formed between IL6 and the soluble IL6 receptor (IL6RIL6), which are capable of mainataining stem cells, and particularly, human embryonic stem cells, in an undifferentiated state are provided. Also provided are cell cultures comprising the culture media and the stem cells and methods of expanding and deriving embryonic stem cells in such well-defined, xeno-free culture media. In addition, the present invention provides methods of differentiating ESCs or EBs formed therefrom for the generation of lineage specific cells.

摘要翻译： 包含TGF-β同种型或在IL6和可溶性IL6受体（IL6RIL6）之间形成的嵌合体的定义良好的，不含异种的培养基，其能够将干细胞，特别是人类胚胎干细胞在未分化的 状态。 还提供了包含培养基和干细胞的细胞培养物以及在这种定义良好的不含异种植物的培养基中扩增和衍生胚胎干细胞的方法。 此外，本发明提供了区分由其形成的ESC或用于产生谱系特异性细胞的EBs的方法。

公开(公告)号：US20130136721A1

公开(公告)日：2013-05-30

申请号：US13577944

申请日：2011-02-09

申请人： Elias Zambidis ,  Paul Burridge

发明人： Elias Zambidis ,  Paul Burridge

IPC分类号： C12N5/071

CPC分类号： C12N5/0602 ,  A61K35/34 ,  C12N5/0657 ,  C12N2500/02 ,  C12N2500/36 ,  C12N2500/38 ,  C12N2500/44 ,  C12N2500/50 ,  C12N2500/84 ,  C12N2500/98 ,  C12N2501/115 ,  C12N2501/155 ,  C12N2501/33 ,  C12N2501/727 ,  C12N2506/02 ,  C12N2506/45

摘要： The invention features a culture system, culture system components and culture methods that are useful for the rapid and reliable generation of differentiated mesodermal cells, including cardiac myocytes, from stem cells, such as human embryonic stem cells and human induced pluripotent stem cells differentiated mesodermal cells, including differentiated cardiac myocytes.

摘要翻译： 本发明的特征在于可用于快速和可靠地产生来自干细胞（例如人胚胎干细胞）和人诱导的多能干细胞分化的中胚层细胞的分化的中胚层细胞（包括心肌细胞）的培养系统，培养系统组分和培养方法 ，包括分化心肌细胞。

公开(公告)号：US20120263689A1

公开(公告)日：2012-10-18

申请号：US13395093

申请日：2010-09-10

申请人： Shigeki Sugii ,  Ronald M. Evans ,  Juan Carlos Izpisua Belmonte ,  Yasuyuki Kida

发明人： Shigeki Sugii ,  Ronald M. Evans ,  Juan Carlos Izpisua Belmonte ,  Yasuyuki Kida

IPC分类号： C12N5/0775 ,  A61K35/12 ,  A61P17/02 ,  C12N15/87

CPC分类号： C12N5/0696 ,  C12N2500/90 ,  C12N2500/98 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/606 ,  C12N2506/1384 ,  C12N2510/00

摘要： Methods and compositions for generating adipose-derived induced pluripotent stem cells for humans and animals and their use are provided.

摘要翻译： 提供用于产生用于人和动物的脂肪来源的诱导性多能干细胞的方法和组合物及其用途。

公开(公告)号：US20120225480A1

公开(公告)日：2012-09-06

申请号：US13508991

申请日：2010-11-11

申请人： Michal Amit ,  Joseph Itskovitz-Eldor

发明人： Michal Amit ,  Joseph Itskovitz-Eldor

IPC分类号： C12N5/071 ,  C12N5/02

CPC分类号： C12N5/0602 ,  C12N5/0606 ,  C12N5/0696 ,  C12N2500/36 ,  C12N2500/90 ,  C12N2500/92 ,  C12N2500/98 ,  C12N2500/99 ,  C12N2501/115 ,  C12N2501/15 ,  C12N2501/23 ,  C12N2506/45

摘要： Provided are serum-free culture media which comprise basic fibroblast growth factor (bFGF), transforming growth factor beta-3, ascorbic acid, xeno-free serum replacement and a lipid mixture. The media may also comprise an IL6R/IL6 chimera, or leukemia inhibitory factor (LIF); wherein the culture medium is capable of maintaining pluripotent stem cells in an undifferentiated state in the absence of feeder cell support. Also provided are cell cultures comprising pluripotent stem cells such as human embryonic stem cells and induced pluripotent stem (iPS) cells, and methods of using the same for expanding pluripotent stem cells in an undifferentiated state using two-dimensional or three-dimensional culture systems. Methods of expanding iPS cells in a suspension culture devoid of substrate adherence and cell encapsulation are also provided.

摘要翻译： 提供了包含碱性成纤维细胞生长因子（bFGF），转化生长因子β-3，抗坏血酸，不含异种血清的血清替代物和脂质混合物的无血清培养基。 培养基还可以包含IL6R / IL6嵌合体或白血病抑制因子（LIF）; 其中所述培养基能够在没有饲养细胞支持的情况下将多能干细胞保持在未分化状态。 还提供了包含多能干细胞（例如人胚胎干细胞和诱导的多能干细胞（iPS））的细胞培养物，以及使用它们用于使用二维或三维培养系统在未分化状态下扩增多能干细胞的方法。 还提供了在没有底物粘附和细胞包封的悬浮培养物中扩增iPS细胞的方法。

公开(公告)号：US08222034B2

公开(公告)日：2012-07-17

申请号：US13083630

申请日：2011-04-11

申请人： Michal Amit ,  Joseph Itskovitz-Eldor

发明人： Michal Amit ,  Joseph Itskovitz-Eldor

IPC分类号： C12N5/02

CPC分类号： C12N5/0606 ,  C12N5/0031 ,  C12N5/0037 ,  C12N5/0043 ,  C12N5/0056 ,  C12N5/0607 ,  C12N5/0611 ,  C12N5/0623 ,  C12N5/0647 ,  C12N5/0696 ,  C12N2500/25 ,  C12N2500/84 ,  C12N2500/90 ,  C12N2500/98 ,  C12N2501/10 ,  C12N2501/115 ,  C12N2501/119 ,  C12N2501/125 ,  C12N2501/15 ,  C12N2501/155 ,  C12N2501/235 ,  C12N2501/33 ,  C12N2501/998 ,  C12N2533/52

摘要： The present invention is of methods of establishing and propagating human embryonic stem cell lines using feeder cells-free, xeno-free culture systems and stem cells which are capable of being maintained in an undifferentiated, pluripotent and proliferative state in culture which is free of xeno contaminants and feeder cells.

摘要翻译： 本发明是使用无饲养细胞，不含异种植物的培养系统和干细胞来建立和繁殖人类胚胎干细胞系的方法，所述培养系统和干细胞能够在不含异种的培养物中保持未分化，多能和增殖状态 污染物和饲养细胞。

公开(公告)号：US20120149025A1

公开(公告)日：2012-06-14

申请号：US13323567

申请日：2011-12-12

申请人： Ramkumar Mandalam ,  Chunhui Xu ,  Joseph D. Gold ,  Melissa K. Carpenter

发明人： Ramkumar Mandalam ,  Chunhui Xu ,  Joseph D. Gold ,  Melissa K. Carpenter

IPC分类号： C12Q1/68 ,  G01N33/566 ,  C12Q1/02

CPC分类号： C12N15/1096 ,  C12N5/0062 ,  C12N5/0068 ,  C12N5/0075 ,  C12N5/0603 ,  C12N5/0606 ,  C12N5/0607 ,  C12N5/0619 ,  C12N5/0622 ,  C12N5/0662 ,  C12N5/0671 ,  C12N5/0672 ,  C12N5/0678 ,  C12N5/068 ,  C12N5/0692 ,  C12N5/0693 ,  C12N5/0696 ,  C12N11/04 ,  C12N15/1034 ,  C12N2500/25 ,  C12N2500/90 ,  C12N2500/98 ,  C12N2500/99 ,  C12N2501/065 ,  C12N2501/105 ,  C12N2501/115 ,  C12N2501/119 ,  C12N2501/125 ,  C12N2501/13 ,  C12N2501/145 ,  C12N2501/155 ,  C12N2501/2306 ,  C12N2501/235 ,  C12N2501/237 ,  C12N2501/26 ,  C12N2501/998 ,  C12N2502/13 ,  C12N2502/99 ,  C12N2503/02 ,  C12N2506/02 ,  C12N2510/00 ,  C12N2510/04 ,  C12N2533/50 ,  C12N2533/90

摘要： This disclosure provides an improved system for culturing human pluripotent stem cells. Traditionally, pluripotent stem cells are cultured on a layer of feeder cells (such as mouse embryonic fibroblasts) to prevent them from differentiating. In the system described here, the role of feeder cells is replaced by components added to the culture environment that support rapid proliferation without differentiation. Effective features are a suitable support structure for the cells, and an effective medium that can be added fresh to the culture without being preconditioned by another cell type. Culturing human embryonic stem cells in fresh medium according to this invention causes the cells to expand surprisingly rapidly, while retaining the ability to differentiate into cells representing all three embryonic germ layers. This new culture system allows for bulk proliferation of pPS cells for commercial production of important products for use in drug screening and human therapy.

摘要翻译： 本公开提供了一种用于培养人多能干细胞的改进系统。 传统上，多能干细胞在一层饲养细胞（如小鼠胚胎成纤维细胞）上培养以防止它们分化。 在这里描述的系统中，饲养细胞的作用由添加到培养环境中的组分代替，其支持快速增殖而不分化。 有效的特征是细胞的合适的支持结构，以及可以在不被另一种细胞类型预处理的情况下可以新鲜添加到培养物中的有效培养基。 在根据本发明的新鲜培养基中培养人胚胎干细胞导致细胞惊人地快速扩张，同时保持分化成代表所有三个胚胎胚层的细胞的能力。 这种新的培养系统允许pPS细胞的大量增殖用于商业生产用于药物筛选和人类治疗的重要产品。

公开(公告)号：US20120121560A1

公开(公告)日：2012-05-17

申请号：US13338453

申请日：2011-12-28

申请人： Shimon Efrat ,  Yael Bar ,  Holger A. Russ

发明人： Shimon Efrat ,  Yael Bar ,  Holger A. Russ

IPC分类号： A61K35/39 ,  C12N5/0775 ,  A61P3/10 ,  C12N5/071

CPC分类号： C12N5/0676 ,  A61K35/12 ,  C12N2500/38 ,  C12N2500/90 ,  C12N2500/98 ,  C12N2500/99 ,  C12N2501/16 ,  C12N2501/335 ,  C12N2501/60 ,  C12N2506/1353

摘要： A method of ex-vivo increasing insulin content in progenitor cells which express SLUG is provided. The method comprises downregulating an amount or activity of SLUG in the progenitor cells. Cell populations generated thereby and uses thereof are also provided.

摘要翻译： 提供了一种离体增加表达SLUG的祖细胞中胰岛素含量的方法。 该方法包括下调祖细胞中SLUG的量或活性。 还提供了由此产生的细胞群体及其用途。

公开(公告)号：US08097458B2

公开(公告)日：2012-01-17

申请号：US12763884

申请日：2010-04-20

申请人： Ramkumar Mandalam ,  Chunhui Xu ,  Joseph D. Gold ,  Melissa K. Carpenter

发明人： Ramkumar Mandalam ,  Chunhui Xu ,  Joseph D. Gold ,  Melissa K. Carpenter

IPC分类号： C12N15/02

CPC分类号： C12N15/1096 ,  C12N5/0062 ,  C12N5/0068 ,  C12N5/0075 ,  C12N5/0603 ,  C12N5/0606 ,  C12N5/0607 ,  C12N5/0619 ,  C12N5/0622 ,  C12N5/0662 ,  C12N5/0671 ,  C12N5/0672 ,  C12N5/0678 ,  C12N5/068 ,  C12N5/0692 ,  C12N5/0693 ,  C12N5/0696 ,  C12N11/04 ,  C12N15/1034 ,  C12N2500/25 ,  C12N2500/90 ,  C12N2500/98 ,  C12N2500/99 ,  C12N2501/065 ,  C12N2501/105 ,  C12N2501/115 ,  C12N2501/119 ,  C12N2501/125 ,  C12N2501/13 ,  C12N2501/145 ,  C12N2501/155 ,  C12N2501/2306 ,  C12N2501/235 ,  C12N2501/237 ,  C12N2501/26 ,  C12N2501/998 ,  C12N2502/13 ,  C12N2502/99 ,  C12N2503/02 ,  C12N2506/02 ,  C12N2510/00 ,  C12N2510/04 ,  C12N2533/50 ,  C12N2533/90

摘要： This disclosure provides an improved system for culturing human pluripotent stem cells. Traditionally, pluripotent stem cells are cultured on a layer of feeder cells (such as mouse embryonic fibroblasts) to prevent them from differentiating. In the system described here, the role of feeder cells is replaced by components added to the culture environment that support rapid proliferation without differentiation. Effective features are a suitable support structure for the cells, and an effective medium that can be added fresh to the culture without being preconditioned by another cell type. Culturing human embryonic stem cells in fresh medium according to this invention causes the cells to expand surprisingly rapidly, while retaining the ability to differentiate into cells representing all three embryonic germ layers. This new culture system allows for bulk proliferation of pPS cells for commercial production of important products for use in drug screening and human therapy.

摘要翻译： 本公开提供了一种用于培养人多能干细胞的改进系统。 传统上，多能干细胞在一层饲养细胞（如小鼠胚胎成纤维细胞）上培养以防止其分化。 在这里描述的系统中，饲养细胞的作用由添加到培养环境中的组分代替，其支持快速增殖而不分化。 有效的特征是细胞的合适的支持结构，以及可以在不被另一种细胞类型预处理的情况下可以新鲜添加到培养物中的有效培养基。 在根据本发明的新鲜培养基中培养人胚胎干细胞导致细胞惊人地快速扩张，同时保持分化成代表所有三个胚胎胚层的细胞的能力。 这种新的培养系统允许pPS细胞的大量增殖用于商业生产用于药物筛选和人类治疗的重要产品。

公开(公告)号：US20110189767A1

公开(公告)日：2011-08-04

申请号：US13083630

申请日：2011-04-11

申请人： MICHAL AMIT ,  JOSEPH ITSKOVITZ-ELDOR

发明人： MICHAL AMIT ,  JOSEPH ITSKOVITZ-ELDOR

IPC分类号： C12N5/0735 ,  C12N5/071

CPC分类号： C12N5/0606 ,  C12N5/0031 ,  C12N5/0037 ,  C12N5/0043 ,  C12N5/0056 ,  C12N5/0607 ,  C12N5/0611 ,  C12N5/0623 ,  C12N5/0647 ,  C12N5/0696 ,  C12N2500/25 ,  C12N2500/84 ,  C12N2500/90 ,  C12N2500/98 ,  C12N2501/10 ,  C12N2501/115 ,  C12N2501/119 ,  C12N2501/125 ,  C12N2501/15 ,  C12N2501/155 ,  C12N2501/235 ,  C12N2501/33 ,  C12N2501/998 ,  C12N2533/52

摘要： The present invention is of methods of establishing and propagating human embryonic stem cell lines using feeder cells-free, xeno-free culture systems and stem cells which are capable of being maintained in an undifferentiated, pluripotent and proliferative state in culture which is free of xeno contaminants and feeder cells.

摘要翻译： 本发明是使用无饲养细胞，不含异种植物的培养系统和干细胞来建立和繁殖人类胚胎干细胞系的方法，所述培养系统和干细胞能够在不含异种的培养物中保持未分化，多能和增殖状态 污染物和饲养细胞。

公开(公告)号：US20110177594A1

公开(公告)日：2011-07-21

申请号：US13005978

申请日：2011-01-13

申请人： Etti Ben SHUSHAN ,  Shelly TANNENBAUM ,  Pavel ITSYKSON ,  Eyal BANIN ,  Benjamin REUBINOFF

发明人： Etti Ben SHUSHAN ,  Shelly TANNENBAUM ,  Pavel ITSYKSON ,  Eyal BANIN ,  Benjamin REUBINOFF

IPC分类号： C12N5/071 ,  C12N5/0735

CPC分类号： C12N5/0606 ,  C12N5/0621 ,  C12N2500/32 ,  C12N2500/38 ,  C12N2500/44 ,  C12N2500/90 ,  C12N2500/98 ,  C12N2500/99 ,  C12N2501/115 ,  C12N2501/155 ,  C12N2501/16 ,  C12N2502/13 ,  C12N2506/02 ,  C12N2533/52

摘要： Provided are systems and methods for providing human cell cultures. Further provided are cultures of feeder cells for use in stem cell technology, as well as cultures, culture systems and methods for maintenance and propagating of stem cells in an undifferentiated state as well as for the development of somatic cells cultures from stem cells, the somatic cell cultures being free of extraembryonic cells.

摘要翻译： 提供了用于提供人类细胞培养物的系统和方法。 进一步提供了用于干细胞技术的饲养细胞的培养物，以及用于维持和繁殖未分化状态的干细胞的培养物，培养系统和方法，以及用于从干细胞培养的体细胞培养物，体细胞 细胞培养物不含胚胎细胞。