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572 条记录 (耗时 0.038 秒)

    Increased production of .beta.-galactosidase in aspergillus oryzae
    82.
    发明授权
    Increased production of .beta.-galactosidase in aspergillus oryzae 失效
    增加米曲霉中β-半乳糖苷酶的产量

    公开(公告)号：US5736374A

    公开(公告)日：1998-04-07

    申请号：US596985

    申请日：1996-02-05

    申请人： Randy M. Berka ,  John A. Hucul ,  Michael Ward

    发明人： Randy M. Berka ,  John A. Hucul ,  Michael Ward

    IPC分类号： C12N15/09 ,  C12N1/15 ,  C12N9/38 ,  C12N15/56 ,  C12R1/69 ,  C07H21/04 ,  C12N1/14 ,  C12N15/00

    CPC分类号： C12Y302/01023 ,  C12N9/2471

    摘要： Novel methods are disclosed for the enhanced expression and secretion of lactase from filamentous fungi. Specifically the novel processes cause enhanced production of lactase from an Aspergillus and preferably enhanced production of A. oryzae lactase from A. oryzae host strains transformed with necessary DNA. Also described are the DNA sequence encoding the lactase gene from A. oryzae and the deduced amino acid sequence of the lactase therefrom.

    摘要翻译： 公开了从丝状真菌增强乳糖酶的表达和分泌的新方法。 具体地，新方法导致来自曲霉的乳糖酶的产生增加，并且优选从用必需DNA转化的米曲霉宿主菌株中增加米曲霉乳糖酶的产生。 还描述了编码米曲霉的乳糖酶基因的DNA序列和由此推断的乳糖酶的氨基酸序列。

    Thermostable, salt tolerant, wide pH range novel chitobiase
    86.
    发明授权
    Thermostable, salt tolerant, wide pH range novel chitobiase 失效
    热稳定性，耐盐性，宽pH范围新型chitobiase

    公开(公告)号：US5693519A

    公开(公告)日：1997-12-02

    申请号：US455837

    申请日：1995-05-31

    申请人： Roger A. Laine ,  Chin-Yih Ou

    发明人： Roger A. Laine ,  Chin-Yih Ou

    IPC分类号： C12N1/21 ,  C12N9/24 ,  C12N15/56 ,  C12N9/42 ,  C12N15/70

    CPC分类号： C12Y302/01052 ,  C12N9/2402 ,  C12Y302/0102

    摘要： A cloned chitobiase from a Vibrio parahemolyticus gene cloned into the plasmid pUC18 in E. coli strain DH5.alpha.. The plasmid construct, called pC120, had a 6.4 kb DNA insert. The recombinant gene expressed chitobiase activity similar to that found in native V. parahemolyticus. In addition to chitobiose, at least six additional substrates were observed to be hydrolyzed by the recombinant chitobiase, including .beta.-N-acetyl galactosamine glycosides, showing that the enzyme is an N-acetyl-hexosaminidase. The enzyme showed resistance to denaturation by 2M NaCl, was thermostable at 45.degree. C., and possessed an unusual range of activity from pH 5 to 9. The enzyme is useful in the degradation of crustacean shells. It catalyzes the production of N-acetyl-glucosamine, a compound which should be valuable as a chiral precursor or intermediate in the synthesis or manufacture of pharmaceutical compounds.

    摘要翻译： 克隆在大肠杆菌菌株DH5α中的质粒pUC18中的来自副溶血弧菌基因的克隆的木质素酶。 称为pC120的质粒构建体具有6.4kb的DNA插入片段。 重组基因表达类似于天然副溶血弧菌中发现的木质素酶活性。 除了壳寡糖之外，观察到至少六个另外的底物被重组壳寡糖酶水解，包括β-N-乙酰半乳糖胺糖苷，显示该酶是N-乙酰基 - 氨基己糖苷酶。 该酶显示出由2M NaCl变性的抗性，在45℃下耐热，并且具有从pH5至9的不寻常的活性范围。该酶可用于甲壳类壳的降解。 它催化N-乙酰葡糖胺的生产，该化合物在药物化合物的合成或制备中应当作为手性前体或中间体有价值。

    Endochitinase gene induced by osmotic stress and abscisic acid isolated from the wild tomato Lycopersicon chilense Dun
    88.
    发明授权
    Endochitinase gene induced by osmotic stress and abscisic acid isolated from the wild tomato Lycopersicon chilense Dun 失效
    由野生番茄分离的渗透胁迫和脱落酸诱导的内切素酶基因番茄番茄

    公开(公告)号：US5656474A

    公开(公告)日：1997-08-12

    申请号：US162475

    申请日：1993-12-07

    申请人： Zohreh Tabaeizadeh ,  Long-Xi Yu ,  Ri-Dong Chen

    发明人： Zohreh Tabaeizadeh ,  Long-Xi Yu ,  Ri-Dong Chen

    IPC分类号： C12N9/24 ,  C12N15/56 ,  C12N9/44

    CPC分类号： C12Y302/01014 ,  C12N9/2442

    摘要： Two osmotic stress- and ABA-responsive members of the endochitinase (EC 3.2.1.14) gene family has been isolated and identified from leaves of drought-stressed Lycopersicon chilense plants. The 966-base-pair insert of pcht28 encodes an acidic chitinase precursor with an amino-terminal signal peptide. The mature protein is predicted to have 229 amino acid residues with a relative molecular weight of 24,943 and pI value of 6.2. The same number in amino acids, molecular and PI value are predicted for the protein encoded by pchtI, despite of a slight variation in the DNA and amino acid sequences. Sequence analysis revealed that pcht28 and pchtI have a high degree of homology with class II chitinases (EC 3.2.1.14) from tobacco. Northern blot analysis indicated that these genes have evolved a completely different pattern of expression from others reported thus far. They are highly induced by both osmotic stress and the plant hormone abscisic acid. Southern blot analysis of genomic DNA suggested that the pcht28- and pchtI-related chitinase is encoded by a small multigene family in this species. Knowing the role of plant chitinase in plant defense against fungal pathogens, it is assumed that, besides their general defensive function, the pcht28- and pchtI-encoded chitinases may play a particular role in protecting plants from pathogen attack during water stress.

    摘要翻译： 已经从干旱胁迫的番茄红叶植物的叶中分离和鉴定了内切壳酶（EC 3.2.1.14）基因家族的两个渗透胁迫和ABA响应成员。 pcht28的966-碱基对插入片段编码具有氨基末端信号肽的酸性几丁质酶前体。 预期成熟蛋白具有229个氨基酸残基，相对分子量为24,943，pI值为6.2。 对于由pchtI编码的蛋白质，预测氨基酸，分子和PI值的相同数量，尽管DNA和氨基酸序列有轻微变化。 序列分析表明，pcht28和pchtI与烟草中的II类几丁质酶（EC 3.2.1.14）具有高度的同源性。 Northern印迹分析表明，这些基因已经演变出与迄今为止报道的其他基因完全不同的表达模式。 它们都被渗透胁迫和植物激素脱落酸所诱导。 基因组DNA的Southern印迹分析表明pcht28-和pchtI相关的几丁质酶由该物种中的一个小型多基因家族编码。 了解植物几丁质酶在植物防御真菌病原体中的作用，假设pcht28和pchtI编码的几丁质酶除了具有一般的防御功能外，在水分胁迫过程中可能起到保护植物免受病原体攻击的特殊作用。

    Chitinase-producing plants
    89.
    发明授权
    Chitinase-producing plants 失效
    几丁质酶生产植物

    公开(公告)号：US5633450A

    公开(公告)日：1997-05-27

    申请号：US566347

    申请日：1995-12-01

    申请人： Trevor V. Suslow ,  Jonathan D. G. Jones

    发明人： Trevor V. Suslow ,  Jonathan D. G. Jones

    IPC分类号： A01N63/00 ,  C12N1/21 ,  C12N9/24 ,  C12N15/56 ,  C12N15/82 ,  A01H5/00 ,  C12N15/29 ,  C12N15/62

    CPC分类号： C12R1/38 ,  A01N63/00 ,  C12N15/8246 ,  C12N15/8273 ,  C12N15/8282 ,  C12N9/2442 ,  C12R1/40 ,  C12Y302/01014

    摘要： Novel plants are described which produce and secrete chitinase as the result of the introduction of foreign DNA linked to a sequence encoding chitinase, an enzyme capable of degrading chitin present in fungi and nematodes. Novel plants that are resistant to cold damage are also described which are created by introduction of DNA encoding for the production of chitinase. The plants of the invention may also have enhanced levels of reducing sugars or sweetness, or produce fruit having enhanced levels of reducing sugars or sweetness, or may be selected for enhanced post-harvest storage life.

    摘要翻译： 描述了产生和分泌几丁质酶的新型植物，其是引入与编码几丁质酶的序列（能够降解存在于真菌和线虫中的几丁质）的酶的外源DNA的结果。 还描述了耐冷伤害的新型植物，其通过引入编码几丁质酶的DNA而产生。 本发明的植物还可以具有增强的还原糖或甜度水平，或产生具有增强的还原糖或甜度水平的水果，或者可以选择用于增强收获后的贮存期限。