公开(公告)号：US20140212470A1

公开(公告)日：2014-07-31

申请号：US14228212

申请日：2014-03-27

申请人： CELLSEED INC.

发明人： Masato KANZAKI ,  Masayuki YAMATO ,  Tinatsu KOHNO ,  Hidekazu SEKINE ,  Akihiko KIKUCHI ,  Teruo OKANO ,  Takamasa ONUKI

IPC分类号： A61L27/38 ,  C12N5/071

CPC分类号： A61L27/3895 ,  A61L27/34 ,  A61L27/3804 ,  A61L27/3839 ,  C12N5/0068 ,  C12N5/0688 ,  C12N2533/30

摘要： The problem to be solved by the present invention is to provide a cultured cell sheet having a superior tissue adhesiveness and an excellent flexibility. The problems can be solved by producing the cultured cell sheet in which cells are cultured in the presence of a surfactant protein or a cross-linking inhibitor on a surface of a cell culture support that is coated with a temperature responsive polymer having an upper or lower critical solution temperature ranging from 0° C. to 80° C. in water, including steps of: adjusting the temperature of the culture medium to a temperature below the lower critical solution temperature or above the upper critical solution temperature; and detaching the cultured cell sheet.

摘要翻译： 本发明要解决的问题是提供具有优异组织粘附性和优异柔韧性的培养细胞片。 可以通过在表面活性蛋白或交联抑制剂的存在下培养细胞培养的细胞片来解决问题，所述细胞片在涂覆有具有上或下的温度响应性聚合物的细胞培养载体的表面上 包括以下步骤：将培养基的温度调节到低于临界溶解温度以下或高于临界溶解温度以上的温度; 并分离培养的细胞片。

公开(公告)号：US07405078B2

公开(公告)日：2008-07-29

申请号：US10221751

申请日：2001-03-16

申请人： Teruo Okano ,  Masayuki Yamato ,  Akihiko Kikuchi

发明人： Teruo Okano ,  Masayuki Yamato ,  Akihiko Kikuchi

IPC分类号： C12N5/00 ,  C12N5/02

CPC分类号： C12N5/0619 ,  C12M35/08 ,  C12N5/0068 ,  C12N5/067 ,  C12N5/0688 ,  C12N2502/1323 ,  C12N2502/28 ,  C12N2535/10 ,  C12N2539/10

摘要： By using a bed material for cell culture having a surface composed of two domains of domain A coated with a temperature-responsive polymer and domain B composed of any one or a combination of a domain coated with a polymer having high affinity with cells, a domain coated with the temperature-responsive polymer in an amount different from the amount of the temperature-responsive polymer of domain A, and a domain coated with a polymer which responds to a temperature different from the temperature to which domain A responds, a method for the co-culture of a plurality of kinds of cells which has heretofore been difficult becomes possible.

摘要翻译： 通过使用具有包覆有温度响应性聚合物的结构域A的两个结构域的表面的细胞培养物的床材料和由涂覆有与细胞具有高亲和力的聚合物的任何一种或其组合组成的结构域B， 涂覆温度响应性聚合物的量不同于结构域A的温度响应性聚合物的量，以及涂覆有聚合物的区域，其响应于与区域A所响应的温度不同的温度， 迄今为止困难的多种细胞的共培养是可能的。

公开(公告)号：US20220257833A1

公开(公告)日：2022-08-18

申请号：US17729608

申请日：2022-04-26

申请人： CellSeed Inc. ,  TOKAI UNIVERSITY EDUCATIONAL SYSTEM

发明人： Masato SATO ,  Chikako SATO ,  Tomomi KOTOKU ,  Tomoko KAWAKE

IPC分类号： A61L27/36 ,  C12N5/0775 ,  C12N5/077 ,  A61L27/38

摘要： Provided is a cell sheet suitable for cartilage repair. The present invention provides a cell sheet for cartilage repair, formed from a culture of cells derived from a cartilage tissue, and the cell sheet is negative for immunostaining using an antibody against type II collagen. The present invention also provides a method for producing a cell sheet for cartilage repair, formed from a culture of cells derived from a cartilage tissue, and the method includes culturing cells derived from a cartilage tissue on a surface of a membrane, where a temperature-responsive polymer is immobilized on the surface, to give the cell sheet. The culturing is stopped before the cell sheet becomes positive for immunostaining using an antibody against type II collagen.

公开(公告)号：US09587222B2

公开(公告)日：2017-03-07

申请号：US10567728

申请日：2004-02-02

申请人： Hikaru Matsuda ,  Yoshiki Sawa ,  Satoshi Taketani ,  Shigeru Miyagawa

发明人： Hikaru Matsuda ,  Yoshiki Sawa ,  Satoshi Taketani ,  Shigeru Miyagawa

IPC分类号： C12N15/00 ,  C12N5/00 ,  C12N5/02 ,  C12N5/071 ,  C12N5/077 ,  A61L27/38 ,  A61K35/34 ,  A61K35/12

CPC分类号： C12N5/0658 ,  A61K35/12 ,  A61K35/34 ,  A61L27/3804 ,  A61L27/3834 ,  A61L27/3839 ,  A61L27/3895 ,  A61L2430/20 ,  C12N5/0068 ,  C12N5/0657 ,  C12N2506/02 ,  C12N2506/1392 ,  C12N2513/00 ,  C12N2523/00 ,  C12N2533/40 ,  C12N2539/10

摘要： The present invention provides a prosthetic tissue or sheet capable of withstanding implantation operations, which can be used in actual operation and can be produced by culture. The present invention also provides a novel therapy which can substitute for cell therapy. Particularly, the present invention provides a method for producing a prosthetic tissue comprising a cell derived from a part other than myocardium and capable of withstanding implantation operation. The above-described objects of the present invention were partially achieved by finding that by culturing cells under specific culture conditions, the cells are unexpectedly organized into a tissue, and the resultant prosthetic tissue is capable of being detached from culture dishes. The present invention also provides a three-dimensional structure applicable to heart, comprising a cell derived from a part other than the myocardium of an adult.

摘要翻译： 本发明提供能够承受植入操作的假体组织或片材，其可以在实际操作中使用并且可以通过培养产生。 本发明还提供了可代替细胞治疗的新型治疗方法。 特别地，本发明提供了一种用于制造假体组织的方法，所述假体组织包含源于除了心肌之外的部分的细胞并且能够承受植入操作。 通过发现通过在特定培养条件下培养细胞，细胞意外地组织成组织，并且所得到的假体组织能够从培养皿中分离，部分实现了本发明的上述目的。 本发明还提供了适用于心脏的三维结构，其包含源自成人心肌之外的部分的细胞。

公开(公告)号：US20140056858A1

公开(公告)日：2014-02-27

申请号：US14001552

申请日：2012-02-28

申请人： Hiroaki Sugiyama ,  Hideaki Sakai ,  Yasuro Shinohara ,  Yasuhiro Takegawa

发明人： Hiroaki Sugiyama ,  Hideaki Sakai ,  Yasuro Shinohara ,  Yasuhiro Takegawa

IPC分类号： C12N5/071 ,  C12N5/079

摘要： A cell sheet having a high hyaluronic acid production ability can be typically prepared by a method in which, on a cell culture support having a surface coated with a polymer which changes a hydration ability in a temperature range of 0-80° C., cells with increased stem cell content are cultured in a temperature range where the polymer exhibits a weak hydration ability, and then the temperature of a culture solution is changed to a temperature at which the polymer exhibits a strong hydration ability, so that the cultured cells are detached in the form of sheet.

摘要翻译： 通常可以通过以下方法制备具有高透明质酸生产能力的细胞片，其中在具有在0-80℃的温度范围内改变水合能力的聚合物表面涂覆的细胞培养支持物的细胞，细胞 在聚合物表现出弱水合能力的温度范围内培养增加的干细胞含量，然后将培养液的温度改变为聚合物表现出强的水合能力的温度，使得培养的细胞分离 以表格的形式。

公开(公告)号：US08642338B2

公开(公告)日：2014-02-04

申请号：US10544542

申请日：2003-02-06

申请人： Masayuki Yamato ,  Teruo Okano

发明人： Masayuki Yamato ,  Teruo Okano

IPC分类号： C12N11/08 ,  C12N5/00 ,  C12N5/02

CPC分类号： C12N5/0621 ,  A61K35/12

摘要： An anterior ocular segment related cell sheet or three-dimensional structure that have only a few structural defects as they have been recovered retaining the intercellular desmosome structure and the basement membrane-like protein between cell and substrate. The anterior ocular segment related cell sheet or three-dimensional structure is produced by a process comprising the steps of cultivating cells on a cell culture support comprising a substrate having its surface covered with a temperature responsive polymer having an upper or lower critical dissolution temperature of 0-80° C. with respect to water, optionally stratifying the layer of cultured cells by the usual method, and thereafter, (1) adjusting the temperature of the culture solution to either above the upper critical dissolution temperature or below the lower critical dissolution temperature, and further optionally (2) bringing the cultured anterior ocular segment related cell sheet or three-dimensional structure into close contact with a polymer membrane, and (3) detaching the sheet or three-dimensional structure together with the polymer membrane.

摘要翻译： 前眼部相关的细胞片或三维结构只有几个结构缺陷，因为它们已经被回收，保留了细胞和基质之间的细胞间质结构和基底膜样蛋白。 前眼部相关细胞片或三维结构的制备方法包括以下步骤：在细胞培养支持物上培养细胞，所述细胞培养载体包含其表面被温度响应性聚合物覆盖的基底，所述温度响应聚合物具有上或下临界溶解温度为0 -80℃，任选地通过常规方法分层培养细胞层，然后，（1）将培养溶液的温度调节至高于上临界溶解温度或低于临界溶解温度 ，进一步任选地（2）使培养的眼前段相关细胞片或三维结构与聚合物膜紧密接触，和（3）与聚合物膜一起分离片或三维结构。

公开(公告)号：US10533158B2

公开(公告)日：2020-01-14

申请号：US11885246

申请日：2006-02-28

申请人： Masato Sato ,  Hideaki Sakai

发明人： Masato Sato ,  Hideaki Sakai

IPC分类号： C12N5/077 ,  A61L27/38 ,  A61K35/12

摘要： The problem to be solved by the present invention is to provide a cultured cell sheet that expresses phenotypes of the chondroid tissue. The problems can be solved by producing the cultured cell sheet in which cells are cultured on a surface of a cell cultureware that is coated with a temperature responsive polymer having an upper or lower critical solution temperature ranging from 0° C. to 80° C. in water, including steps of: adjusting the temperature of the culture medium to a temperature below the lower critical solution temperature or above the upper critical solution temperature; bringing the cultured cell sheet in close contact with the carrier; and detaching the cultured cell sheet together with the carrier.

公开(公告)号：US10368980B2

公开(公告)日：2019-08-06

申请号：US15098604

申请日：2016-04-14

申请人： Tokyo Women's Medical University ,  The Jikei University ,  CellSeed Inc.

发明人： Takanori Hama ,  Hiromi Kojima ,  Hiroshi Moriyama ,  Masayuki Yamato ,  Teruo Okano

IPC分类号： A61F2/18 ,  C12N5/0793 ,  A61L27/38 ,  A61K35/12

摘要： The purpose of the present invention is to obtain an alternative to a substitute of the mucosa in the middle ear which is engrafted on the surface of the bone in the middle ear, hyperplasia of the granulation tissue and the bone and the development of the fibroblast cells in the middle ear are suppressed, and to obtain a middle ear mucosa-like cell sheet retaining cilia in the surface layer, comprising culturing nasal epithelium cells on a cell culture substrate coated with a polymer whose hydration force changes within a temperature range of 0 to 80° C., wherein the cells are cultured within a temperature range where the hydration force of the polymer is weak, and then changing the temperature to a temperature at which the hydration force is strong to recover the cultured cell sheet.

公开(公告)号：US20140024124A1

公开(公告)日：2014-01-23

申请号：US13985677

申请日：2012-02-16

申请人： Yasuro Shinohara ,  Yasuhiro Takegawa ,  Naoki Fujitani ,  Jun-ichi Furukawa ,  Hideaki Sakai

发明人： Yasuro Shinohara ,  Yasuhiro Takegawa ,  Naoki Fujitani ,  Jun-ichi Furukawa ,  Hideaki Sakai

IPC分类号： G01N33/68

CPC分类号： G01N33/6842 ,  G01N30/7233 ,  G01N33/6848 ,  G01N33/6851 ,  G01N2030/8836 ,  G01N2440/00

摘要： A glycoprotein and/or a glycopeptide which are a test substance is heated in the presence of a pyrazolone derivative, an isoxazolone derivative, a hydantoin derivative, a rhodanine derivative, a maleimide derivative, or the like under a basic condition to cleave and label a post-translational modification group for analysis, thereby enabling analysis of a post-translational modification of a serine residue and/or a threonine residue.

摘要翻译： 作为试验物质的糖蛋白和/或糖肽在碱性条件下在吡唑啉酮衍生物，异恶唑酮衍生物，乙内酰脲衍生物，绕丹宁衍生物，马来酰亚胺衍生物等存在下加热，以切割并标记 翻译后修饰组进行分析，从而能够分析丝氨酸残基和/或苏氨酸残基的翻译后修饰。

公开(公告)号：US07632656B2

公开(公告)日：2009-12-15

申请号：US10547751

申请日：2004-03-04

申请人： Hideko Kanazawa ,  Teruo Okano

发明人： Hideko Kanazawa ,  Teruo Okano

IPC分类号： C12Q1/26

CPC分类号： G01N33/57438 ,  G01N33/573 ,  G01N33/6893

摘要： The amount of drug consumption caused by a specific drug-metabolizing enzyme and/or the amount of the resulting metabolite(s) is measured by chromatography with an aqueous mobile phase under temperature control using a packing material whose surface is coated with a polymer having a hydration force varying in the temperature range of 0° C. to 80° C. This system enables proper evaluation of drug-metabolizing capacity through simple means without adversely affecting the environment.

摘要翻译： 由特定药物代谢酶引起的药物消耗量和/或所得代谢物的量通过使用包装材料的水性流动相的色谱法进行测量，所述包装材料的表面涂覆有具有 水合力在0°C至80°C的温度范围内变化。该系统可以通过简单的手段适当评估药物代谢能力，而不会对环境产生不利影响。