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    Coronamycins
    1.
    发明授权
    Coronamycins 失效
    冠心病

    公开(公告)号：US07387888B2

    公开(公告)日：2008-06-17

    申请号：US11064486

    申请日：2005-02-24

    申请人： Gary A. Strobel ,  Debbie S. Yaver ,  Uvidelio F. Castillo ,  David Ezra

    发明人： Gary A. Strobel ,  Debbie S. Yaver ,  Uvidelio F. Castillo ,  David Ezra

    IPC分类号： C12C1/00

    CPC分类号： C12R1/465 ,  A01N63/00 ,  A01N63/02 ,  A61K38/00 ,  C07K14/36 ,  C12P21/00

    摘要： The present invention relates to isolated strains of a Streptomyces spp. which are endophytes of dicotyledonous plants and to methods for selecting such strains. The present invention also relates to a biologically active compound called coronamycin obtained from endophytic Streptomycetes isolated from higher plants. The present invention further relates to compositions of such compounds and to methods of protecting plants against attack by a plant pathogen and methods of inhibiting bacterial growth, fungal growth, viral infection, growth of parasitic organisms, and cancer cell growth with such compositions.

    摘要翻译： 本发明涉及链霉菌属（Streptomyces spp。）的分离菌株。 其是双子叶植物的内生真菌和选择这些菌株的方法。 本发明还涉及从高等植物分离的内生菌链球菌获得的称为冠状病毒的生物活性化合物。 本发明进一步涉及这些化合物的组合物以及保护植物免受植物病原体攻击的方法以及用这些组合物抑制细菌生长，真菌生长，病毒感染，寄生生物生长和癌细胞生长的方法。

    ENDOPHYTIC STREPTOMYCETES FROM HIGHER PLANTS WITH BIOLOGICAL ACTIVITY
    2.
    发明申请
    ENDOPHYTIC STREPTOMYCETES FROM HIGHER PLANTS WITH BIOLOGICAL ACTIVITY 失效

    公开(公告)号：US20070190633A1

    公开(公告)日：2007-08-16

    申请号：US10406006

    申请日：2003-04-03

    申请人： Gary Strobel ,  Uvidelio Castillo

    发明人： Gary Strobel ,  Uvidelio Castillo

    IPC分类号： C12N1/20

    CPC分类号： A01N63/00 ,  A01H17/00 ,  C07K4/04 ,  C12R1/465

    摘要： The present invention relates to isolated strains of a Streptomyces spp. which are endophytes of dicotyledonous plants and to methods for selecting such strains. The present invention also relates to compounds having biological activity selected from the group consisting of munumbicin A, munumbicin B, munumbicin C and munumbicin D, kakadumycin A, kakadumycin B, and kakadumycin C. The present invention further relates to compositions of such compounds and to methods of protecting plants against attack by a plant pathogen and methods of inhibiting bacterial growth, fungal growth, viral infection, growth of parasitic organisms, and cancer cell growth with such compositions.

    Methods for degrading or converting plant cell wall polysaccharides
    3.
    发明申请
    Methods for degrading or converting plant cell wall polysaccharides 有权
    降解或转化植物细胞壁多糖的方法

    公开(公告)号：US20050233423A1

    公开(公告)日：2005-10-20

    申请号：US11078921

    申请日：2005-03-10

    申请人： Randy Berka ,  Joel Cherry

    发明人： Randy Berka ,  Joel Cherry

    IPC分类号： C07G99/00 ,  C12P5/00 ,  C12P5/02 ,  C12P7/00 ,  C12P7/02 ,  C12P7/04 ,  C12P7/06 ,  C12P7/10 ,  C12P7/16 ,  C12P7/18 ,  C12P7/20 ,  C12P7/26 ,  C12P7/28 ,  C12P7/40 ,  C12P13/04 ,  C12P17/04 ,  C12P19/02 ,  C12P19/04 ,  C13B35/00

    CPC分类号： C12P19/14 ,  C12P5/023 ,  C12P7/00 ,  C12P7/02 ,  C12P7/04 ,  C12P7/06 ,  C12P7/10 ,  C12P7/16 ,  C12P7/18 ,  C12P7/20 ,  C12P7/26 ,  C12P7/28 ,  C12P7/40 ,  C12P13/04 ,  C12P17/04 ,  C12P19/02 ,  Y02E50/10 ,  Y02E50/16 ,  Y02E50/17 ,  Y02E50/343

    摘要： The present invention relates to methods for converting plant cell wall polysaccharides into one or more products, comprising: treating the plant cell wall polysaccharides with an effective amount of a spent whole fermentation broth of a recombinant microorganism, wherein the recombinant microorganism expresses one or more heterologous genes encoding enzymes which degrade or convert the plant cell wall polysaccharides into the one or more products. The present invention also relates to methods for producing an organic substance, comprising: (a) saccharifying plant cell wall polysaccharides with an effective amount of a spent whole fermentation broth of a recombinant microorganism, wherein the recombinant microorganism expresses one or more heterologous genes encoding enzymes which degrade or convert the plant cell wall polysaccharides into saccharified material; (b) fermenting the saccharified material of step (a) with one or more fermenting microoganisms; and (c) recovering the organic substance from the fermentation.

    摘要翻译： 本发明涉及用于将植物细胞壁多糖转化成一种或多种产品的方法，其包括：用有效量的重组微生物的消耗的全发酵液处理植物细胞壁多糖，其中所述重组微生物表达一种或多种异源 编码将植物细胞壁多糖降解或转化成一种或多种产物的酶的基因。 本发明还涉及有机物质的制造方法，其特征在于，包括：（a）用有效量的重组微生物用的全部发酵肉汤糖化植物细胞壁多糖，其中所述重组微生物表达一种或多种编码酶的异源基因 其将植物细胞壁多糖降解或转化为糖化物质; （b）用一种或多种发酵微生物发酵步骤（a）的糖化物质; 和（c）从发酵中回收有机物质。

    Methods for isolating genes from microorganisms
    4.
    发明授权
    Methods for isolating genes from microorganisms 失效
    从微生物分离基因的方法

    公开(公告)号：US06942973B2

    公开(公告)日：2005-09-13

    申请号：US10095975

    申请日：2002-03-12

    申请人： Debbie S. Yaver ,  Randy M. Berka

    发明人： Debbie S. Yaver ,  Randy M. Berka

    IPC分类号： C12N15/10 ,  C12N15/30 ,  C12N15/52 ,  C12N15/74 ,  C12Q1/68 ,  C07H21/04 ,  C12N9/08 ,  C12P21/02

    CPC分类号： C12Q1/6895 ,  C12N15/1034 ,  C12Q1/6809 ,  C12Q2600/158 ,  C12Q2565/501 ,  C12Q2565/102 ,  C12Q2525/179

    摘要： The present invention relates to methods for isolating a gene encoding an enzyme, comprising: (a) adding a mixture of labeled first nucleic acid probes from a microbial strain cultured on medium without the substrate, and labeled second nucleic acid probes from a microbial strain cultured on medium with the substrate, to an array of random nucleic acid fragments of the microbial strain where the labeled nucleic acids hybridize to complementary sequences of the genomic fragments in the array, wherein the first nucleic acid probes are labeled with a first reporter and the second nucleic acid probes are labeled with a second reporter; (b) examining the array under conditions wherein the relative expression of the genes of the microbial strain is determined by the observed hybridization reporter signal of each spot in the array; and (c) isolating a gene from the microbial strain that encodes an enzyme that degrades the substrate. The present invention also relates to isolated genes obtained by such methods.

    摘要翻译： 本发明涉及用于分离编码酶的基因的方法，其包括：（a）从培养基上培养的微生物菌株中加入标记的第一核酸探针的混合物，而不含底物，以及来自培养的微生物菌株的标记的第二核酸探针 在具有底物的培养基上的微生物菌株的随机核酸片段的阵列，其中标记的核酸与阵列中的基因组片段的互补序列杂交，其中第一核酸探针用第一报道分子标记， 核酸探针用第二报告物标记; （b）在条件下检查阵列，其中微生物菌株的基因的相对表达通过阵列中每个斑点的观察杂交报道信号确定; 和（c）从编码降解底物的酶的微生物菌株中分离基因。 本发明还涉及通过这些方法获得的分离基因。

    Methods for identifying markers of antimicrobial compounds
    6.
    发明申请
    Methods for identifying markers of antimicrobial compounds 审中-公开
    识别抗微生物化合物标记物的方法

    公开(公告)号：US20050074844A1

    公开(公告)日：2005-04-07

    申请号：US10656055

    申请日：2003-09-05

    申请人： Debbie Yaver ,  Alan Sloma

    发明人： Debbie Yaver ,  Alan Sloma

    IPC分类号： A23J3/34 ,  C12N1/20 ,  C12P21/06 ,  C12Q1/18 ,  C12Q1/68

    CPC分类号： C12Q1/6809 ,  A23J3/343 ,  C12Q1/18 ,  C12Q1/689 ,  C12Q2600/136 ,  C12Q2600/158

    摘要： The present invention relates to arrays for bacterial gene expression profiling in response to treatment with antimicrobial compounds. Bacterial nucleic acid molecules are used as the hybridizable elements in various applications, such as arrays for gene expression profiling and for determining a mode of action for an antimicrobial compound. The present invention also relates to nucleic acid sequences which can be developed as targets for drug screening.

    摘要翻译： 本发明涉及用抗菌化合物处理的细菌基因表达谱的阵列。 细菌核酸分子用作各种应用中的可杂交元件，例如用于基因表达谱的阵列和用于确定抗微生物化合物的作用模式。 本发明还涉及可作为药物筛选靶标开发的核酸序列。

    Microbial trypsin mutants having chymotrypsin activity and nucleic acids encoding same
    7.
    发明申请
    Microbial trypsin mutants having chymotrypsin activity and nucleic acids encoding same 失效
    具有糜蛋白酶活性的微生物胰蛋白酶突变体和编码相同的核酸

    公开(公告)号：US20050037368A1

    公开(公告)日：2005-02-17

    申请号：US10669757

    申请日：2003-09-24

    申请人： Alan Klotz ,  Kimberly Brown ,  Elizabeth Zaretsky

    发明人： Alan Klotz ,  Kimberly Brown ,  Elizabeth Zaretsky

    IPC分类号： C12N9/58 ,  C12Q1/68 ,  C07H21/04 ,  C12N9/76 ,  C12N15/74

    CPC分类号： C12N9/58

    摘要： The present invention relates to microbial trypsin variants having chymotrypsin-like activity, comprising: (a) a one or more substitutions corresponding to positions 144, S193A, 198, 201, 218, 223, 227, 228, 229, 230, and 231 of amino acids 25 to 248 of SEQ ID NO: 2, (b) one or more deletions corresponding to positions 192, 197, and 226 of amino acids 25 to 248 of SEQ ID NO: 2; and (c) an insertion between positions corresponding to positions 224 and 225 of amino acids 25 to 248 of SEQ ID NO: 2. The present invention further relates to nucleotide sequences encoding microbial trypsin variants having chymotrypsin-like activity; nucleic acid constructs, expression vectors, and recombinant host cells comprising such nucleotide sequences; and methods of producing microbial trypsin variants having chymotrypsin-like activity or a precursor thereof.

    摘要翻译： 本发明涉及具有胰凝乳蛋白酶样活性的微生物胰蛋白酶变体，其包含：（a）对应于位置144，S193A，198,201,218,223,227,228,229,230和231的一个或多个取代 SEQ ID NO：2的氨基酸25至248，（b）对应于SEQ ID NO：2的氨基酸25至248的位置192,197和226的一个或多个缺失; 本发明还涉及编码具有胰凝乳蛋白酶样活性的微生物胰蛋白酶变体的核苷酸序列;以及（c）相应于SEQ ID NO：2的氨基酸25至248的位置224和225的位置之间的插入。 核酸构建体，表达载体和包含此类核苷酸序列的重组宿主细胞; 以及产生具有胰凝乳蛋白酶样活性的微生物胰蛋白酶变体或其前体的方法。

    Methods for producing secreted polypeptides
    8.
    发明申请
    Methods for producing secreted polypeptides 失效
    产生分泌多肽的方法

    公开(公告)号：US20040248258A1

    公开(公告)日：2004-12-09

    申请号：US10837318

    申请日：2004-04-30

    申请人： Novozymes Biotech, Inc.

    发明人： Suchindra Maiyuran ,  Ana Fidantsef ,  Howard Brody

    IPC分类号： C12N001/18 ,  C12P021/02 ,  C07K014/47

    CPC分类号： C12N15/81 ,  C07K2319/02 ,  C12N15/80 ,  C12P21/02

    摘要： The present invention relates to methods for producing a polypeptide, comprising: (a) cultivating a fungal host cell in a medium conducive for the production of the polypeptide, wherein the fungal host cell comprises a nucleic acid construct comprising a first nucleotide sequence encoding a signal peptide operably linked to a second nucleotide sequence encoding the polypeptide, wherein the first nucleotide sequence is foreign to the second nucleotide sequence and the 3null end of the first nucleotide sequence is immediately upstream of the initiator codon of the second nucleotide sequence. The present invention also relates to the isolated signal peptide sequences and to constructs, vectors, and fungal host cells comprising the signal peptide sequences operably linked to nucleotide sequences encoding polypeptides.

    摘要翻译： 本发明涉及产生多肽的方法，包括：（a）在有助于产生多肽的培养基中培养真菌宿主细胞，其中真菌宿主细胞包含核酸构建体，其包含编码信号的第一核苷酸序列 肽，其与编码多肽的第二核苷酸序列可操作地连接，其中第一个核苷酸序列与第二个核苷酸序列是异源的，第一个核苷酸序列的3'末端紧邻第二个核苷酸序列的起始密码子的上游。 本发明还涉及分离的信号肽序列以及包含与编码多肽的核苷酸序列可操作地连接的信号肽序列的构建体，载体和真菌宿主细胞。