公开(公告)号：US07932486B2

公开(公告)日：2011-04-26

申请号：US12330374

申请日：2008-12-08

申请人： Akihiro Sano ,  Atsumu Hirabayashi ,  Yasushi Terui ,  Kinya Kobayashi ,  Kiyomi Yoshinari ,  Kenko Uchida ,  Toshiyuki Yokosuka

发明人： Akihiro Sano ,  Atsumu Hirabayashi ,  Yasushi Terui ,  Kinya Kobayashi ,  Kiyomi Yoshinari ,  Kenko Uchida ,  Toshiyuki Yokosuka

IPC分类号： H01J49/26

CPC分类号： G01N33/6851 ,  G01N33/6848 ,  H01J49/0031 ,  H01J49/0036 ,  H01J49/004

摘要： During the structural analysis of a protein or peptide by tandem mass spectroscopy, a peptide ion derived from a protein that has already been measured and that is expressed in great quantities is avoided as a tandem mass spectroscopy target. A peptide derived from a minute amount of protein, which has heretofore been difficult to analyze, can be automatically determined as a tandem mass spectroscopy target within the real time of measurement. Data concerning a protein that has already been measured and a peptide derived from the protein is automatically stored in an internal database. The stored data is collated with measured data with high accuracy to determine an isotope peak. In this way, the process of selecting a peptide peak that has not been measured as the target for the next tandem analysis can be performed within the real time of measurement and a redundant measurement of peptides derived from the same protein can be avoided. The information contained in the MSn spectrum is effectively utilized in each step of the MSn involving a multi-stage dissociation and mass spectroscopy (MSn), so that the flows for the determination of the next analysis content and the selection of the parent ion for the MSn+1 analysis, for example, can be optimized within the real time of measurement and with high efficiency and accuracy. Thus, a target of concern to the user can be subjected to tandem mass spectroscopy without wasteful measurement.

摘要翻译： 在通过串联质谱法对蛋白质或肽进行结构分析期间，作为串联质谱目标，可以避免衍生自已经测量并且大量表达的蛋白质的肽离子。 迄今为止难以分析的，从微量蛋白质衍生的肽可以在实时测定中作为串联质谱图目标自动确定。 关于已经测量的蛋白质和来自蛋白质的肽的数据被自动存储在内部数据库中。 存储的数据与测量数据高精度对照以确定同位素峰。 以这种方式，可以在测量的实际时间内进行未被测定为下一个串联分析的靶标的肽峰的过程，并且可以避免衍生自相同蛋白质的肽的冗余测量。 包含在MSn谱中的信息在涉及多级解离和质谱（MSn）的MSn的每个步骤中被有效地利用，使得用于确定下一个分析内容的流程和为母体离子的选择 例如，MSn + 1分析可以在实时测量和高效率和精确度下进行优化。 因此，用户关注的目标可以进行串联质谱，无需浪费测量。

公开(公告)号：US20080048110A1

公开(公告)日：2008-02-28

申请号：US11878241

申请日：2007-07-23

申请人： Kisaburo DEGUCHI ,  Atsumu HIRABAYASHI ,  Hiroki ITO ,  Takashi BABA

发明人： Kisaburo DEGUCHI ,  Atsumu HIRABAYASHI ,  Hiroki ITO ,  Takashi BABA

IPC分类号： H01J49/00

CPC分类号： G01N33/6848 ,  H01J49/004

摘要： To analyze glycans and peptide sequences without liberating glycans from glycosylated peptides, a glycan structure is analyzed through negative-ion CID, in which sialic acid and fucose are resistant to elimination, and a peptide sequence is analyzed through positive-ion ECD.

摘要翻译： 为了分析聚糖和肽序列而不从糖基化肽释放聚糖，通过负离子CID分析聚糖结构，其中唾液酸和岩藻糖对消除具有抗性，并且通过正离子ECD分析肽序列。

公开(公告)号：US06989531B2

公开(公告)日：2006-01-24

申请号：US10875635

申请日：2004-06-25

申请人： Atsumu Hirabayashi ,  Masako Ishimaru ,  Masahiro Yamaoka

发明人： Atsumu Hirabayashi ,  Masako Ishimaru ,  Masahiro Yamaoka

IPC分类号： H01J49/10

CPC分类号： H01J49/167 ,  G01N30/7266

摘要： The invention relates to an LC/MS interface provided with structure that the end of a spray capillary is rarely damaged and the object is to provide the LC/MS interface which a general user can easily operate, which inhibits the extension of a separation band and the deterioration of detection sensitivity and which enables high separation and sensitive analysis. A metallic member having a hole for connecting a separation column and a spray capillary and a resin member for fixing the upstream side of the short spray capillary are used. Further, to enhance operability, spray ionization using gas spraying is used.

摘要翻译： 本发明涉及一种LC / MS接口，其具有喷雾毛细管的端部很少被损坏的结构，并且目的是提供一般用户可以容易地操作的LC / MS接口，这阻碍了分离带的扩展， 检测灵敏度的恶化，能够进行高分离和敏感分析。 使用具有用于连接分离柱和喷雾毛细管的孔的金属构件和用于固定短喷雾毛细管的上游侧的树脂构件。 此外，为了提高可操作性，使用使用气体喷雾的喷雾电离。

公开(公告)号：US06478738B1

公开(公告)日：2002-11-12

申请号：US09695019

申请日：2000-10-25

申请人： Yukiko Hirabayashi ,  Atsumu Hirabayashi ,  Hideaki Koizumi

发明人： Yukiko Hirabayashi ,  Atsumu Hirabayashi ,  Hideaki Koizumi

IPC分类号： A61B500

CPC分类号： H01J49/0431 ,  A61B5/145 ,  A61B5/14528 ,  A61B5/14557 ,  A61B5/1459 ,  A61B5/4064 ,  A61B2010/0077

摘要： A living body fluid mass-analyzing method introducing a first solution whose osmotic pressure is close to that of a living body fluid into a microdialysis, extracting the living body fluid from the microdialysis, mixing the living body fluid from the microdialysis and an organic solution, and applying a gas around a capillary through which the mixed living fluid flows thereby spraying and ionizing the living body fluid by the gas. Further, the ionized ions are introduced into three dimensional mass-analyzer through an orifice, accumulating the ions in said three dimensional mass-analyzer and sending out the accumulated ions in order of mass to measure mass numbers of the ions.

摘要翻译： 一种生物体液分析方法，其将渗透压接近生物体液的第一溶液引入微透析，从微透析提取生物体液，混合来自微透析的活体液和有机溶液， 并且在毛细管周围施加气体，混合的生物流体通过该毛细管喷射并通过气体电离生物体液。 此外，通过孔口将离子化离子引入三维质量分析器中，将离子积聚在所述三维质量分析器中，并以质量的顺序排出积聚的离子以测量离子的质量数。

公开(公告)号：US06446883B1

公开(公告)日：2002-09-10

申请号：US09651132

申请日：2000-08-30

申请人： Min Huang ,  Atsumu Hirabayashi

发明人： Min Huang ,  Atsumu Hirabayashi

IPC分类号： B05B706

CPC分类号： B05B7/0876 ,  B05B7/066 ,  B05B7/0892 ,  G01N21/714 ,  Y10T436/24

摘要： Disclosed herein is a nebulizer capable of performing spraying over a wide flow-rate range from a low flow rate to a high flow rate stably and with high efficiency. Further, the present invention provides a supersonic nebulizer capable of improving the efficiency of spraying by a supersonic region spray gas, and a supersonic array nebulizer wherein a plurality of spray units are placed in array form.

摘要翻译： 本发明公开了一种喷雾器，其能够在从低流量到高流量的高流量范围内以稳定且高效率的方式进行喷雾。 此外，本发明提供了能够提高由超声波区域喷雾气体喷射的效率的超音速雾化器和其中多个喷射单元以阵列形式放置的超音速阵列雾化器。

公开(公告)号：US06437327B2

公开(公告)日：2002-08-20

申请号：US09859508

申请日：2001-05-18

申请人： Yasuaki Takada ,  Minoru Sakairi ,  Atsumu Hirabayashi ,  Takayuki Nabeshima ,  Hideaki Koizumi

发明人： Yasuaki Takada ,  Minoru Sakairi ,  Atsumu Hirabayashi ,  Takayuki Nabeshima ,  Hideaki Koizumi

IPC分类号： H01J4910

CPC分类号： H01J49/165 ,  G01N30/7266 ,  H01J49/168

摘要： A mass spectrometer including a sprayer for spraying a sample solution, a needle electrode for ionizing the sample contained in the sample solution, an aperture for introducing the ions of the sample into a lower pressure region having a pressure lower than the pressure of a region in which the needle electrode is disposed, and a mass spectrometric apparatus for analyzing the ions introduced from the aperture. A direction of a spray caused by the sprayer is almost perpendicular to or at an angle with respect to a center axis of the aperture, and a tip of the needle is disposed facing to an electrode having the aperture.

摘要翻译： 一种质谱仪，包括用于喷射样品溶液的喷雾器，用于使包含在样品溶液中的样品电离的针状电极，用于将样品的离子引入低于区域压力的低压区域的孔 设置针状电极的质谱仪和用于分析从孔径引入的离子的质谱仪。 由喷雾器引起的喷雾方向几乎与孔的中心轴垂直或成一定角度，并且针的尖端面向具有孔的电极。

公开(公告)号：US6114693A

公开(公告)日：2000-09-05

申请号：US299638

申请日：1999-04-27

申请人： Atsumu Hirabayashi ,  Yukiko Hirabayashi ,  Minoru Sakairi ,  Yasuaki Takada ,  Takayuki Nabeshima ,  Hideaki Koizumi

发明人： Atsumu Hirabayashi ,  Yukiko Hirabayashi ,  Minoru Sakairi ,  Yasuaki Takada ,  Takayuki Nabeshima ,  Hideaki Koizumi

IPC分类号： G01N27/62 ,  G01N27/447 ,  G01N30/72 ,  H01J49/04 ,  H01J49/10

CPC分类号： G01N30/7266 ,  H01J49/0468 ,  H01J49/167

摘要： A mass spectrometer comprising a passage through which a sample solution flows, the passage including a capillary having an open tip, the sample solution flowing through the capillary out of the open tip of the capillary, a gas passage which forms a gas flow around the open tip of the capillary, the gas flow spraying the sample solution flowing out of the open tip of the capillary, a first electrode which contacts the sample solution flowing in the passage and applies an electric potential to the sample solution flowing in the passage, a second electrode disposed around the capillary near the open tip of the capillary, the second electrode being downstream from the first electrode with respect to a direction in which the sample solution flows in the passage, there being no gap extending downstream from the open tip of the capillary to the second electrode in a direction in which the sample solution flows out of the open tip of the capillary, the second electrode being electrically isolated from the sample solution flowing in the capillary and applying an electric field to the sample solution flowing in the capillary, and an analyzer which analyzes a mass of gaseous ions formed from the sample solution sprayed by the gas flow.

摘要翻译： 一种质谱仪，包括样品溶液流过的通道，所述通道包括具有开口尖端的毛细管，所述样品溶液从毛细管的开口尖端流过毛细管，气体通道围绕开口形成气流 毛细管的尖端，气流喷射从毛细管的开口尖端流出的样品溶液，第一电极，其接触在通道中流动的样品溶液，并向在通道中流动的样品溶液施加电位，第二电极 设置在毛细管附近的毛细管周围的电极，第二电极相对于样品溶液在通道中流动的方向从第一电极下游，在毛细管的开口尖端的下游没有间隙 在样品溶液从毛细管的开口尖端流出的方向到第二电极，第二电极是电隔离的 从在毛细管中流动的样品溶液流出并向在毛细管中流动的样品溶液施加电场，以及分析器，其分析由气流喷射的样品溶液形成的气态离子的质量。

公开(公告)号：US07544930B2

公开(公告)日：2009-06-09

申请号：US11624248

申请日：2007-01-18

申请人： Kiyomi Yoshinari ,  Yasushi Terui ,  Toshiyuki Yokosuka ,  Kinya Kobayashi ,  Atsumu Hirabayashi

发明人： Kiyomi Yoshinari ,  Yasushi Terui ,  Toshiyuki Yokosuka ,  Kinya Kobayashi ,  Atsumu Hirabayashi

IPC分类号： H01J49/00

CPC分类号： H01J49/02 ,  H01J49/004

摘要： The present invention provides a tandem type mass analysis system capable of carrying out the differential analysis with high efficiency by the tandem type mass analysis. A predetermined number of m/z regions are set up for carrying out the mass analysis with the all ions included therein being dissociated collectively for each m/z region so as to obtain measurement MS2 data. By comparing the measurement MS2 data with reference MS2 data stored in a reference data base, a difference thereof is detected. For the m/z region with a differential component detected, the mass analysis is carried out collectively without dissociation for the all ions included therein so as to obtain measurement MS1 data. By comparing the measurement MS1 data with the reference MS1 data, a difference thereof is detected. From the difference thereof, a parent ion considered to be the differential component factor is presumed for carrying out the mass analysis with the same being dissociated.

摘要翻译： 本发明提供一种能够通过串联型质量分析以高效率进行差示分析的串联式质量分析系统。 建立预定数量的m / z区域以进行质量分析，其中包含的全部离子为每个m / z区域共同解离，以获得测量MS2数据。 通过将测量MS2数据与参考数据库中存储的参考MS2数据进行比较，检测其差异。 对于检测到差分成分的m / z区域，对于其中包含的所有离子，不分离地进行质量分析，以获得测量MS1数据。 通过将测量MS1数据与参考MS1数据进行比较，检测其差异。 从它们的差异来看，推测认为是差分成分因子的母离子用于进行相同解离的质量分析。

公开(公告)号：US20080237459A1

公开(公告)日：2008-10-02

申请号：US12081625

申请日：2008-04-18

申请人： Atsumu Hirabayashi ,  Minoru Sakairi ,  Yasuaki Takada ,  Hideaki Koizumi ,  Kaoru Umemura

发明人： Atsumu Hirabayashi ,  Minoru Sakairi ,  Yasuaki Takada ,  Hideaki Koizumi ,  Kaoru Umemura

IPC分类号： B01D59/44

CPC分类号： H01J49/045

摘要： An ion source includes a body having a gas passage and an orifice. A capillary is inserted into the gas passage so that a tip portion of the capillary extends into the orifice. A gas supplier supplies a gas into the gas passage to form a gas flow through the gas passage along the capillary and through the orifice past a tip of the capillary so that the gas flow sprays a sample solution flowing through the capillary from the tip of the capillary. A flow controller regulates a pressure of the gas in the gas passage to adjust a characteristic value F/S to a predetermined value, where F is a flow rate of the gas flow at standard conditions (20.degree. C., 1 atmosphere), and S is a difference between a cross section of the orifice and a cross section of the tip portion of the capillary in the orifice.

摘要翻译： 离子源包括具有气体通道和孔的主体。 毛细管插入到气体通道中，使得毛细管的尖端部分延伸到孔中。 气体供应商将气体供应到气体通道中以形成沿着毛细管的气体通道的气流，并且通过孔经过毛细管的尖端，使得气流从其中的尖端喷射流过毛细管的样品溶液 毛细管。 流量控制器调节气体通道中的气体的压力，以将特性值F / S调节到预定值，其中F是在标准条件（20℃，1个大气压）下的气体流量的流量， ，S是孔口的截面与孔口中的毛细管的前端部的截面的差。

公开(公告)号：US20050092917A1

公开(公告)日：2005-05-05

申请号：US10875635

申请日：2004-06-25

申请人： Atsumu Hirabayashi ,  Masako Ishimaru ,  Masahiro Yamaoka

发明人： Atsumu Hirabayashi ,  Masako Ishimaru ,  Masahiro Yamaoka

IPC分类号： G01N27/62 ,  H01J49/04

CPC分类号： H01J49/167 ,  G01N30/7266

摘要： The invention relates to an LC/MS interface provided with structure that the end of a spray capillary is rarely damaged and the object is to provide the LC/MS interface which a general user can easily operate, which inhibits the extension of a separation band and the deterioration of detection sensitivity and which enables high separation and sensitive analysis. A metallic member having a hole for connecting a separation column and a spray capillary and a resin member for fixing the upstream side of the short spray capillary are used. Further, to enhance operability, spray ionization using gas spraying is used.

摘要翻译： 本发明涉及一种LC / MS接口，其具有喷雾毛细管的端部很少被损坏的结构，并且目的是提供一般用户可以容易地操作的LC / MS接口，这阻碍了分离带的扩展， 检测灵敏度的恶化，能够进行高分离和敏感分析。 使用具有用于连接分离柱和喷雾毛细管的孔的金属构件和用于固定短喷雾毛细管的上游侧的树脂构件。 此外，为了提高可操作性，使用使用气体喷雾的喷雾电离。