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    cDNA library preparation
    5.
    发明申请
    cDNA library preparation 审中-公开
    cDNA文库制备

    公开(公告)号：US20070117121A1

    公开(公告)日：2007-05-24

    申请号：US11523120

    申请日：2006-09-18

    申请人： Stephen Hutchison ,  Jan Simons ,  David Willoughby

    发明人： Stephen Hutchison ,  Jan Simons ,  David Willoughby

    IPC分类号： C40B30/06 ,  C40B40/08

    CPC分类号： C12N15/1096

    摘要： New biochemical protocols for high throughput processing of mRNA samples into cDNA libraries with adaptor sequences compatible with automated sequencing systems are provided. The provided methods produces cDNA libraries which do not have 3′ bias associated with current cDNA library production methods. New methods for the production of DNA libraries from DNA are also provided.

    摘要翻译： 提供了用于将mRNA样品高通量处理的新的生物化学方案转化为具有与自动测序系统相容的衔接子序列的cDNA文库。 所提供的方法产生不具有与当前cDNA文库生产方法相关的3'偏差的cDNA文库。 还提供了用于从DNA生产DNA文库的新方法。

    Method, system, and product for providing extended error handling capability in host bridges
    6.
    发明申请
    Method, system, and product for providing extended error handling capability in host bridges 有权
    方法，系统和产品，用于在主机桥中提供扩展的错误处理能力

    公开(公告)号：US20050081126A1

    公开(公告)日：2005-04-14

    申请号：US10682401

    申请日：2003-10-09

    申请人： Ashwini Kulkarni ,  Douglas Oliver ,  Steven Vongvibool ,  David Willoughby

    发明人： Ashwini Kulkarni ,  Douglas Oliver ,  Steven Vongvibool ,  David Willoughby

    IPC分类号： G06F9/455 ,  G06F11/00 ,  G06F11/22

    CPC分类号： G06F11/0712 ,  G06F11/0745 ,  G06F11/0793 ,  G06F11/2284

    摘要： A method, system, and computer program product in a logical partitioned data processing system are disclosed for providing a host bridge that implements extended error handling (EEH). If all devices coupled to the host bridge implement EEH, the host bridge is initialized to operate in EEH mode. In EEH mode, the devices handle any error that occurs within the devices without reporting the error to the host bridge. All partitions that share the host bridge continue to operate without being terminated while the devices are handling the error. If at least one device does not implement EEH, the host bridge is initialized to operate in non-EEH mode. In non-EEH mode, a machine check is generated by the host bridge when an error occurs within one of the devices resulting in the termination of all partitions that share the host bridge in response to a receipt of the machine check.

    摘要翻译： 公开了一种用于提供实现扩展错误处理（EEH）的主机桥的逻辑分区数据处理系统中的方法，系统和计算机程序产品。 如果耦合到主桥的所有设备都实现EEH，则主桥被初始化为在EEH模式下工作。 在EEH模式下，设备会处理设备中发生的任何错误，而不会向主机桥报告错误。 共享主机桥的所有分区在设备处理错误时继续运行，而不会终止。 如果至少有一个设备不实现EEH，则主桥将被初始化为在非EEH模式下工作。 在非EEH模式下，当在一个设备内发生错误时，由主桥产生机器检查，导致响应于机器检查的接收而共享主桥的所有分区的终止。

    Novel Process for Construction of a DNA Library
    7.
    发明申请
    Novel Process for Construction of a DNA Library 审中-公开
    DNA文库构建的新过程

    公开(公告)号：US20070031865A1

    公开(公告)日：2007-02-08

    申请号：US11456082

    申请日：2006-07-06

    申请人： David Willoughby

    发明人： David Willoughby

    IPC分类号： C40B30/06 ,  C40B40/08

    CPC分类号： C12N15/1093 ,  C12N15/1096

    摘要： The invention is directed to processes for constructing DNA Libraries in which ssDNA containing a chemical modification (CM) at or near the 5′- or 3′-terminus is prepared from a RNA or DNA source, a 1st universal oligonucleotide (Oligo A′) is ligated to the 3′-of the ssDNA, and a 2nd universal oligonucleotide (Oligo B) is ligated to the 5′-terminus of the ssDNA. Chemical modifications useful for the process are functional groups capable of binding a solid support with high affinity, or functional groups that can mediate a non-enzymatic ligation. In one embodiment of the invention, a CM at or near the 5′-terminus of the ssDNA mediates binding of the ssDNA to a solid support, allowing removal of residual unligated Oligo A′ prior to ligation of Oligo B. In another embodiment of the invention, a CM at or near the 5′-terminus of ssDNA mediates non-enzymatic ligation of Oligo B to the 5′-terminus of ssDNA, under conditions in which no further ligation of Oligo A′ can occur. Libraries prepared by the method of the invention can be directly amplified by PCR or other methods. Amplified libraries, derived from minute quantities of RNA and DNA, can be used in gene expression studies, analysis of DNA polymorphisms, and high throughput sequencing. Methods of attaching the finished DNA Libraries to a solid supports for archiving are also disclosed. The invention further provides kits for carrying out the processes of the invention.

    摘要翻译： 本发明涉及用于构建DNA文库的方法，其中由RNA或DNA来源制备含有在5'或3'末端或其附近的化学修饰（CM）的ssDNA，1' 将通用寡核苷酸（Oligo A'）连接到ssDNA的3'-末端，并将2'通用寡核苷酸（Oligo B）连接到ssDNA的5'末端。 可用于该方法的化学修饰是能够以高亲和力结合固体支持物的官能团，或可介导非酶连接的官能团。 在本发明的一个实施方案中，在ssDNA的5'端或其附近的CM介导ssDNA与固体支持物的结合，允许在连接Oligo B之前除去残留的未结合的Oligo A'。在另一个实施方案中 在ssDNA的5'端或其附近的CM在不再进一步连接Oligo A'的条件下介导Oligo B与ssDNA的5'末端的非酶连接。 通过本发明方法制备的文库可以通过PCR或其他方法直接扩增。 衍生自微量RNA和DNA的扩增文库可用于基因表达研究，DNA多态性分析和高通量测序。 还公开了将完成的DNA文库附着到用于归档的固体支持物的方法。 本发明还提供了用于实施本发明方法的试剂盒。