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    Tetrahymena bifunctional dihydrofolate reductase-thymidylate synthase deficiency and its use
    2.
    发明申请
    Tetrahymena bifunctional dihydrofolate reductase-thymidylate synthase deficiency and its use 有权
    四膜双功能二氢叶酸还原酶 - 胸苷酸合酶缺陷及其用途

    公开(公告)号：US20100021952A1

    公开(公告)日：2010-01-28

    申请号：US11989717

    申请日：2006-09-20

    Applicant: Thomas Weide ,  Ulrike Bockau ,  Marcus Hartmann ,  Lutz Herrmann

    Inventor： Thomas Weide ,  Ulrike Bockau ,  Marcus Hartmann ,  Lutz Herrmann

    IPC: C12N9/02 ,  C12N15/79 ,  C12N1/10 ,  C12Q1/26 ,  C07H21/00

    CPC classification number: C12N9/003

    Abstract: A method for producing a ciliate cell with reduced or essentially no dihydrofolate reductase (DHFS) activity or reduced or essentially no thymidylate synthase (TS) activity or both reduced or essentially no dihydrofolate reductase and thymidylate synthase (DHFR-TS) activity is claimed, comprising the steps of a) transforming ciliate cells by inserting a construct containing an allele altering the gene encoding the endogenous DHFR-TS into at least one of the endogenous DHFR-TS genes of the ciliate macronucleus (MAC), b) inducing an allelic assortment process in the transformed ciliate cells to generate cells having the construct inserted in most or all functional DHFR-TS genes of the MAC, and c) identifying the cells generated in step b) by cultivation with or without thymidine.

    Abstract translation: 一种生产具有降低或基本上不含二氢叶酸还原酶（DHFS）活性或降低或基本上不含胸苷酸合酶（TS）活性或两者还原或基本上不含二氢叶酸还原酶和胸苷酸合酶（DHFR-TS）活性）的细胞细胞的方法，包括 以下步骤：a）通过将含有等位基因的构建体插入到编码内源性DHFR-TS的基因至少一个内源性DHFR-TS基因的细胞核中（MAC），b）诱导等位基因分类过程 在转化的细胞细胞中产生具有插入到MAC的大部分或全部功能性DHFR-TS基因中的构建体的细胞，以及c）通过用或不用胸苷培养来鉴定步骤b）中产生的细胞。

    Tetrahymena Heat Inducible Promoters and Their Use
    3.
    发明申请
    Tetrahymena Heat Inducible Promoters and Their Use 有权
    四膜虫热诱导启动子及其用途

    公开(公告)号：US20080261290A1

    公开(公告)日：2008-10-23

    申请号：US11922348

    申请日：2006-07-13

    Applicant: Marcus Hartmann ,  Thomas Weide ,  Lutz Herrmann ,  Nadine Niebur

    Inventor： Marcus Hartmann ,  Thomas Weide ,  Lutz Herrmann ,  Nadine Niebur

    IPC: C12N1/11 ,  C07H21/04

    CPC classification number: C07K14/44 ,  C12N2830/002

    Abstract: The present invention relates to heat-inducible promoters of the heat shock protein family of the ciliate Tetrahymena thermophila, especially a promoter having the nucleotide sequence of Seq. ID No. 1 or promoter-effective fragments thereof.Furthermore the use of heat-inducible promoters of the present invention for the expression of homologous and/or heterologous proteins in the ciliate Tetrahymena thermophila is claimed.

    Abstract translation: 本发明涉及嗜热四膜虫嗜热细菌的热休克蛋白家族的热诱导型启动子，特别是具有Seq的核苷酸序列的启动子。 1号或其启动子有效片段。 此外，要求使用本发明的热诱导型启动子在嗜中性四膜虫嗜热细菌中表达同源和/或异源蛋白质。

    Dna sequences of major secreted proteins from the ciliate tetrahymena and use thereof
    4.
    发明申请
    Dna sequences of major secreted proteins from the ciliate tetrahymena and use thereof 审中-公开
    来自附属四膜虫的主要分泌蛋白质的Dna序列及其用途

    公开(公告)号：US20060127973A1

    公开(公告)日：2006-06-15

    申请号：US10507908

    申请日：2003-03-19

    Applicant: Marcus Hartmann ,  Nadine Wolf

    Inventor： Marcus Hartmann ,  Nadine Wolf

    IPC: C07K14/195 ,  C07H21/04 ,  C12P21/06 ,  C12N1/21 ,  C12N15/74

    CPC classification number: C12N15/79 ,  C07K14/44 ,  C12P21/02

    Abstract: A regulatory element of a DNA for an efficient heterologous expression of proteins in Tetrahymena ssp which efficient heterologous expression is performed under control of promotors and/or terminators which are derived from in Tetrahymena ssp naturally occurring DNA comprising promotors and/or terminators and a coding region for proteins secreted ion a high level and the expression of proteins secreted on a high level is independent of the cell-cycle of Tetrahymena ssp. Furthermore a method is disclosed for the heterologous expression of proteins from Tetrahymena using gene constructs made from regulatory elements selected from the group consisting of promoters or terminators from Tetrahymena and coding nucleic acid sequences of a protein to be expressed heterologously, said regulatory elements from Tetrahymena being obtainable by: two-dimensional gel electrophoretical separation and isolation of the proteins (CMSP) selected from the group according to table 1; determination of at least one partial amino acid sequence of the proteins; establishing the nucleic acid sequence of the proteins and therefrom establishing the gene which codes for these proteins; establishing the regulatory elements of the coding region of said proteins.

    Abstract translation: 用于在四膜虫ssp中有效异源表达蛋白质的DNA的调节元件，其在来自四膜虫ssp天然存在的DNA中的启动子和/或终止子的控制下进行有效的异源表达，所述天然存在的DNA包含启动子和/或终止子和编码区 对于分泌高蛋白的蛋白质，高水平分泌的蛋白质的表达与四膜虫的细胞周期无关。 此外，公开了使用由选自四膜虫的启动子或终止子的调节元件构建的基因构建体和来自四膜虫的表达的蛋白质的编码核酸序列的来自四膜虫的蛋白质的异源表达的方法，所述调节元件来自四膜虫 可通过以下方法获得：二维凝胶电泳分离和分离从表1中选择的蛋白质（CMSP）; 确定蛋白质的至少一个部分氨基酸序列; 建立蛋白质的核酸序列，从而建立编码这些蛋白质的基因; 建立所述蛋白质编码区的调节元件。

    Capacitance Measuring Circuit
    7.
    发明申请
    Capacitance Measuring Circuit 有权
    电容测量电路

    公开(公告)号：US20080191713A1

    公开(公告)日：2008-08-14

    申请号：US11915183

    申请日：2006-05-24

    Applicant: Johann Hauer ,  Stefan Moedl ,  Marcus Hartmann

    Inventor： Johann Hauer ,  Stefan Moedl ,  Marcus Hartmann

    IPC: G01R27/26

    CPC classification number: H03M3/496 ,  G01R27/2605 ,  H03M3/43 ,  H03M3/456

    Abstract: The present invention is based on the finding that a capacitance can be measured precisely and efficiently when, in a delta-sigma modulator having an operational amplifier, a first capacitor connectable to an input of the operational amplifier, and a second capacitor in a feedback branch of the operational amplifier, a reference signal source is connectable to the first capacitor, wherein the first or second capacitor may represent a capacitance to be measured. Due to the fact that, in contrast to what is conventional, no input quantity is measured and digitalized at the input of the delta-sigma modulator, but instead a defined reference signal source is coupled at the input and a device of the delta-sigma modulator itself represents the measuring quantity, an extremely compact circuit is provided allowing capacitances to be measured quickly and reliably, the measuring result being additionally made available in a digital form.

    Abstract translation: 本发明基于以下发现：当在具有运算放大器的Δ-Σ调制器中可连接到运算放大器的输入端的第一电容器和反馈支路中的第二电容器时，可以精确地和有效地测量电容 所述运算放大器的参考信号源可连接到所述第一电容器，其中所述第一或第二电容器可表示要测量的电容。 由于与常规技术相比，在Δ-Σ调制器的输入端没有测量和数字化输入量，而是将定义的参考信号源耦合在输入端和delta-sigma的器件 调制器本身表示测量量，提供了一种非常紧凑的电路，允许电容快速可靠地测量，测量结果另外以数字形式提供。

    Screening method for identifying protease secretion-deficient mutants of microorganisms
    9.
    发明授权
    Screening method for identifying protease secretion-deficient mutants of microorganisms 有权
    用于鉴定微生物的蛋白酶分泌缺陷突变体的筛选方法

    公开(公告)号：US08309321B2

    公开(公告)日：2012-11-13

    申请号：US13027591

    申请日：2011-02-15

    Applicant: Marcus Hartmann ,  Andre Broermann

    Inventor： Marcus Hartmann ,  Andre Broermann

    IPC: C12Q1/37

    CPC classification number: C12Q1/37 ,  C12Q1/04

    Abstract: A method for identifying a protease secretion deficient strain of a microorganism involves producing a mutant of the microorganism, followed by adding the mutant to gel-filled wells of a microtitration plate, incubating the mutant in the gel-filled wells under conditions call sing the mutant to secrete proteins, separating the mutant from the gel, and measuring activity of a secretion protease of the microorganism in the gel, wherein either (i) the gel is a substrate for the secretion protease or (ii) the gel contains a substrate for the secretion protease.

    Abstract translation: 用于鉴定微生物的蛋白酶分泌缺陷菌株的方法涉及产生微生物的突变体，然后将突变体加入到微量滴定板的凝胶填充的孔中，在凝胶填充的孔中将突变体温育在称为突变体 分泌蛋白质，从凝胶中分离突变体，以及测定凝胶中微生物分泌蛋白酶的活性，其中（i）凝胶是分泌蛋白酶的底物，或（ii）凝胶含有用于 分泌蛋白酶。

    Nucleic acid and its use effecting ciliate tetrahymena bifunctional dihydrofolate reductase-thymidylate synthase deficiency
    10.
    发明授权
    Nucleic acid and its use effecting ciliate tetrahymena bifunctional dihydrofolate reductase-thymidylate synthase deficiency 有权
    核酸及其使用影响细胞四膜双功能二氢叶酸还原酶 - 胸苷酸合酶缺乏症

    公开(公告)号：US08114650B2

    公开(公告)日：2012-02-14

    申请号：US11989717

    申请日：2006-09-20

    Applicant: Thomas Weide ,  Ulrike Bockau ,  Marcus Hartmann ,  Lutz Herrmann

    Inventor： Thomas Weide ,  Ulrike Bockau ,  Marcus Hartmann ,  Lutz Herrmann

    IPC: C07H21/00 ,  C12N15/00 ,  C12P21/00 ,  C12Q1/26 ,  C12Q1/68 ,  C12N9/02 ,  C07K14/00

    CPC classification number: C12N9/003

    Abstract: A method for producing a ciliate cell with reduced or essentially no dihydrofolate reductase (DHFS) activity or reduced or essentially no thymidylate synthase (TS) activity or both reduced or essentially no dihydrofolate reductase and thymidylate synthase (DHFR-TS) activity is claimed, comprising the steps of a) transforming ciliate cells by inserting a construct containing an allele altering the gene encoding the endogenous DHFR-TS into at least one of the endogenous DHFR-TS genes of the ciliate macronucleus (MAC), b) inducing an allelic assortment process in the transformed ciliate cells to generate cells having the construct inserted in most or all functional DHFR-TS genes of the MAC, and c) identifying the cells generated in step b) by cultivation with or without thymidine.

    Abstract translation: 一种生产具有降低或基本上不含二氢叶酸还原酶（DHFS）活性或降低或基本上不含胸苷酸合酶（TS）活性或两者还原或基本上不含二氢叶酸还原酶和胸苷酸合酶（DHFR-TS）活性）的细胞细胞的方法，包括 以下步骤：a）通过将含有等位基因的构建体插入到编码内源性DHFR-TS的基因至少一个内源性DHFR-TS基因的细胞核中（MAC），b）诱导等位基因分类过程 在转化的细胞细胞中产生具有插入到MAC的大部分或全部功能性DHFR-TS基因中的构建体的细胞，以及c）通过用或不用胸苷培养来鉴定步骤b）中产生的细胞。

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