利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

24 条记录 (耗时 0.034 秒)

    Monomers useful in the preparation of separation matrices
    3.
    发明授权
    Monomers useful in the preparation of separation matrices 失效
    用于制备分离基质的单体

    公开(公告)号：US07060776B2

    公开(公告)日：2006-06-13

    申请号：US10499884

    申请日：2002-12-17

    申请人： Mattias Algotsson ,  Bengt Bjellqvist ,  Ronnie Palmgren

    发明人： Mattias Algotsson ,  Bengt Bjellqvist ,  Ronnie Palmgren

    IPC分类号： C08F226/02

    CPC分类号： C07C233/20 ,  C08F20/56 ,  G01N27/44747

    摘要： The invention relates to an acrylic monomer defined by formula (I): wherein R1, R2 and R3 are H or CH3; R4 and R5 are H or OH; R6 and R7 are H or OH; R8 is H or CH3; m is 2–4; and n and p are 0 or 1. The invention also relates to a polymer comprised of repeated such monomer units as well as a separation matrix prepared thereof.

    摘要翻译： 本发明涉及由式（I）定义的丙烯酸单体：其中R 1，R 2和R 3均为H或CH 3 ; R 4和R 5是H或OH; R 6和R 7是H或OH; R 8是H或CH 3; m为2-4; 并且n和p为0或1.本发明还涉及由重复的这种单体单元以及由其制备的分离基质组成的聚合物。

    NOVEL CHELATOR AND USE THEREOF
    6.
    发明申请
    NOVEL CHELATOR AND USE THEREOF 有权
    新任教士及其使用

    公开(公告)号：US20130072638A1

    公开(公告)日：2013-03-21

    申请号：US13701074

    申请日：2011-05-30

    申请人： Mattias Algotsson ,  Lars C. Andersson ,  Eggert Brekkan ,  Lee Hedemyr ,  Jean-Luc Maloisel ,  Ronnie Palmgren

    发明人： Mattias Algotsson ,  Lars C. Andersson ,  Eggert Brekkan ,  Lee Hedemyr ,  Jean-Luc Maloisel ,  Ronnie Palmgren

    IPC分类号： C08B37/00 ,  B01J20/281

    CPC分类号： C08B37/0039 ,  B01J20/281 ,  B01J20/289 ,  B01J20/3219 ,  B01J20/3251 ,  B01J20/3265 ,  C07C237/10 ,  C07C323/60

    摘要： The present invention relates to dimeric pentadentate chelators with exceptionally strong binding of metal ions, for detection, immobilization and purification of biomolecules. Dimeric chelators offer a cooperativity of binding of two adjacent immobilized metal ions simultaneously to a histidine-tagged biomolecule, which gives advantageous properties regarding strength of binding compared to a corresponding monomer chelator. In addition, a dimer increases the selectivity (ease of separation) against non-tagged biomolecules with low metal-ion affinity.The dimeric pentadentate chelator according to the invention has the following general formula: whereinSc is a scaffold or a connecting structure that contains at least two functional groups enabling coupling of two pentadentate chelators (PD), andPD is a pentadentate chelator having the formula:

    摘要翻译： 本发明涉及具有非常强的金属离子结合的二聚五齿螯合物，用于生物分子的检测，固定和纯化。 二聚螯合剂提供了两个相邻的固定化金属离子与组氨酸标记的生物分子的结合的协同作用，与对应的单体螯合剂相比，其对结合强度具有有利的性质。 此外，二聚体增加了具有低金属离子亲和力的非标记生物分子的选择性（易于分离）。 根据本发明的二聚五齿螯合剂具有以下通式：其中Sc是含有至少两个能够偶合两种五齿螯合剂（PD）的官能团的支架或连接结构，PD是具有下式的五齿螯合剂：

    Electrophoretic support
    8.
    发明申请
    Electrophoretic support 审中-公开
    电泳支持

    公开(公告)号：US20070051630A1

    公开(公告)日：2007-03-08

    申请号：US10555236

    申请日：2004-05-25

    申请人： Anders Larsson ,  Ronnie Palmgren ,  Sofia Söderberg

    发明人： Anders Larsson ,  Ronnie Palmgren ,  Sofia Söderberg

    IPC分类号： G01N27/00

    CPC分类号： G01N27/44747

    摘要： The present invention relates to electrophoresis and in particular low fluorescent electrophoretic supports for hydrogels used for separation of fluorescence labelled biomolecules. More particularly, the invention relates to use of a polymer having the following formula: wherein n=0-100 000 R1, R2, R3 and R4=H, F, Cl, Br, I, methyl groups or non-aromatic hydrocarbon chains (optionally containing branches or cyclic structures) such as ethyl, ethenyl, propyl, isopropyl, propenyl, butyl, branched butyl, butenyl, cyclobutyl, pentyl, branched pentyl, pentenyl, cyclopentyl, hexyl, branched hexyl, cyclohexyl; X, Y=methylene groups or non-aromatic hydrocarbon chains (optionally containing branches or cyclic structures) such as ethylene, ethenylene, propylene, isopropylene, propenylene, butylene, branched butylene, butenylene; Y can optionally be absent as a low fluorescent support film of an electrophoretic hydrogel for slab gel electrophoresis. The invention also relates to composites of such films and hydrogels as well as kits for 2D electrophoresis.

    摘要翻译： 本发明涉及电泳，特别是用于分离荧光标记的生物分子的水凝胶的低荧光电泳载体。 更具体地说，本发明涉及具有下式的聚合物的用途：其中n = 0-100000 R1，R2，R3和R4 = H，F，Cl，Br，I，甲基或非芳族烃链（ 丙烯基，异丙基，丙烯基，丁基，支链丁基，丁烯基，环丁基，戊基，支链戊基，戊烯基，环戊基，己基，支链己基，环己基; X，Y =亚甲基或非芳族烃链（任选含有支链或环状结构）如乙烯，亚乙烯基，丙烯，异丙烯，亚丙烯基，亚丁基，支链亚丁基，亚丁烯基; 作为板状凝胶电泳用电泳水凝胶的低荧光支持膜，Y可任选地不存在。 本发明还涉及这种膜和水凝胶的复合材料以及用于2D电泳的试剂盒。

    Method and kit for the manufacture of separation gels
    9.
    发明授权
    Method and kit for the manufacture of separation gels 有权
    用于制造分离凝胶的方法和试剂盒

    公开(公告)号：US07052590B1

    公开(公告)日：2006-05-30

    申请号：US10110020

    申请日：2000-10-12

    申请人： Lena Stigh ,  Ronnie Palmgren

    发明人： Lena Stigh ,  Ronnie Palmgren

    IPC分类号： G01N27/26 ,  G01N27/447

    CPC分类号： C08F26/02 ,  C08F2/48 ,  C08F220/02 ,  G01N27/44704 ,  G01N27/44747 ,  C08F222/385

    摘要： A method for the manufacture of a separation gel, which comprises the steps: (i) providing a polymerization mixture containing a first monomer (I) having a polymerizable unsaturated structure and a second monomer (II) comprising two or more polymerizable unsaturated structures and an initiator, and (ii) polymerizing the mixture. The method is characterized in that monomer (I) is an N-vinyl carboxamide; and that (a) the initiating system for the polymerization comprises a UV photoinitiator and polymerization is initiated by UV irradiating the mixture at a wave-length appropriate for the initiator, and/or (b) preselecting monomer (I) and monomer (II) to have comparable reactivities to become incorporated into the growing polymer. A kit for use in the production of an electrophoretic gel is also described. The kit comprises: (a) a monomer (I) carrying an N-vinyl carboxamide group; (b) a monomer (II) carrying a polymerizable unsaturated structure; and (c) an UV initiator compound, a buffer and/or a denaturing agent; said monomers (I and II) being selected to provide comparable reactivities.

    摘要翻译： 一种制备分离凝胶的方法，其包括以下步骤：（i）提供含有具有可聚合不饱和结构的第一单体（I）和包含两个或多个可聚合不饱和结构的第二单体（II）的聚合混合物，和 引发剂，和（ii）使混合物聚合。 该方法的特征在于单体（I）是N-乙烯基甲酰胺; 并且（a）聚合引发体系包括UV光引发剂，并通过以适于引发剂的波长UV照射混合物和/或（b）预选择单体（I）和单体（II））来引发聚合反应， 具有可比较的反应性以变成掺入生长的聚合物中。 还描述了用于生产电泳凝胶的试剂盒。 试剂盒包括：（a）携带N-乙烯基酰胺基团的单体（I）; （b）携带可聚合不饱和结构的单体（II）; 和（c）UV引发剂化合物，缓冲剂和/或变性剂; 所述单体（I和II）被选择以提供可比较的反应性。

    Polymeric support having novel pore structures
    10.
    发明申请
    Polymeric support having novel pore structures 失效
    具有新颖孔结构的聚合物载体

    公开(公告)号：US20050245624A1

    公开(公告)日：2005-11-03

    申请号：US10517226

    申请日：2003-06-17

    申请人： Philippe Busson ,  Ronnie Palmgren ,  Michael Morrison

    发明人： Philippe Busson ,  Ronnie Palmgren ,  Michael Morrison

    IPC分类号： B01J20/281 ,  B01J20/26 ,  B01J20/285 ,  C08F2/18 ,  C08F4/00 ,  C08F4/40 ,  C08F12/08 ,  C08F12/36 ,  C08J9/26 ,  C08J9/00

    CPC分类号： B01J20/28092 ,  B01J20/26 ,  B01J20/267 ,  B01J20/28004 ,  B01J20/28019 ,  B01J20/28042 ,  B01J20/28057 ,  B01J20/28061 ,  B01J20/28097 ,  B01J20/285 ,  B01J20/3064 ,  B01J2220/82 ,  C08F2/18 ,  C08J9/26

    摘要： The invention is a method of producing a cross-linked polymeric support having a multimodal pore structure, which comprises providing a degradable initiator molecule; providing an organic phase comprising said initiator molecule, radically polymerisable monomers and a porogen in a solvent; providing an aqueous phase comprising a transition metal catalyst; suspension polymerisation of the organic phase by adding a ligand, co-ordinating to the transition metal in the aqueous phase to produce a cross-linked polymeric support having a primary pore structure and comprising initiator molecule; and subjecting the support obtained to degrading conditions to remove the initiator molecule from within the support to produce a cross-linked polymeric support having a secondary pore structure in addition to the primary pore structure.

    摘要翻译： 本发明是一种生产具有多峰孔结构的交联聚合物载体的方法，其包括提供可降解的引发剂分子; 提供包含所述引发剂分子，可自由基聚合的单体和在溶剂中的致孔剂的有机相; 提供包含过渡金属催化剂的水相; 通过添加配体来悬浮聚合有机相，与水相中的过渡金属配位以产生具有一次孔结构并包含引发剂分子的交联聚合物载体; 并使获得的载体降解条件从支持体内除去引发剂分子，以产生除主孔结构之外具有二次孔结构的交联聚合物载体。