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公开(公告)号：US09593160B2
公开(公告)日：2017-03-14
申请号：US14233553
申请日：2012-07-18
申请人： Donald E. Ingber , Michael Super , Jeffrey Charles Way , Mark J. Cartwright , Julia B. Berthet , Dinah R. Super , Martin M. Rottman , Alexander Watters
发明人： Donald E. Ingber , Michael Super , Jeffrey Charles Way , Mark J. Cartwright , Julia B. Berthet , Dinah R. Super , Martin M. Rottman , Alexander Watters
IPC分类号： C07K14/42 , C07K16/12 , A61K47/48 , G01N33/543 , G01N33/569
CPC分类号： C07K16/12 , A61K47/68 , A61K47/6815 , C07K14/42 , C07K2319/33 , G01N33/54353 , G01N33/56911 , G01N33/56916 , G01N33/56938 , G01N33/56961 , G01N2333/42 , G01N2333/4724 , Y02A50/53 , Y02A50/58
摘要： Described herein are engineered microbe-targeting or microbe-binding molecules, kits comprising the same and uses thereof. Some particular embodiments of the microbe-targeting or microbe-binding molecules comprise a carbohydrate recognition domain of mannose-binding lectin, or a fragment thereof, linked to a portion of a Fc region. In some embodiments, the microbe-targeting molecules or microbe-binding molecules can be conjugated to a substrate, e.g., a magnetic microbead, forming a microbe-targeting substrate (e.g., a microbe-targeting magnetic microbead). Such microbe-targeting molecules and/or substrates and the kits comprising the same can bind and/or capture of a microbe and/or microbial matter thereof, and can thus be used in various applications, e.g., diagnosis and/or treatment of an infection caused by microbes such as sepsis in a subject or any environmental surface. Microbe-targeting molecules and/or substrates can be regenerated after use by washing with a low pH buffer or buffer in which calcium is insoluble.
摘要翻译： 本文描述了工程化的微生物靶向或微生物结合分子，包含其的试剂盒及其用途。 微生物靶向或微生物结合分子的一些具体实施方案包括与Fc区的一部分连接的甘露糖结合凝集素或其片段的碳水化合物识别结构域。 在一些实施方案中，微生物靶向分子或微生物结合分子可以与基底（例如磁性微珠）缀合，形成微生物靶向底物（例如，微生物靶向磁性微珠）。 这样的微生物靶向分子和/或底物及其包含的试剂盒可以结合和/或捕获微生物和/或其微生物物质，因此可用于各种应用中，例如诊断和/或治疗感染 由微生物引起，如受试者或任何环境表面的败血症。 微生物靶向分子和/或底物可以在使用后通过用不溶于钙的低pH缓冲液或缓冲液洗涤而再生。
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公开(公告)号：US20160311877A1
公开(公告)日：2016-10-27
申请号：US15105298
申请日：2014-12-18
申请人： Alexander WATTERS , Donald INGBER , Mark CARTWRIGHT , Michael SUPER , Martin ROTTMAN , PRESIDENT AND FELLOWS OF HARVARD COLLEGE
发明人： Alexander WATTERS , Donald INGBER , Mark CARTWRIGHT , Michael SUPER , Martin ROTTMAN , Evangelina MURRAY , Brendon DUSEL
IPC分类号： C07K14/47 , A61K47/42 , G01N33/569
摘要： Described herein are engineered microbe-targeting molecules, microbe-targeting articles, kits comprising the same, and uses thereof. Such microbe-targeting molecules, microbe-targeting articles, or the kits comprising the same can bind or capture of a microbe or microbial matter thereof, and can thus be used in various applications, such as diagnosis or treatment of an infection caused by microbes in a subject or any environmental surface.
摘要翻译： 本文描述了工程化的微生物靶向分子，微生物靶向制品，包含其的试剂盒及其用途。 这样的微生物靶向分子，微生物靶向制品或包含其的试剂盒可以结合或捕获其微生物或微生物物质，因此可以用于各种应用中，例如诊断或治疗由微生物引起的感染 主题或任何环境表面。
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公开(公告)号：US08691952B2
公开(公告)日：2014-04-08
申请号：US11648505
申请日：2006-12-29
CPC分类号： C07K16/30 , A61K39/3955 , A61K2039/505 , C07K16/2803 , C07K16/2866 , C07K16/2896 , C07K2317/24 , C07K2317/41 , C07K2317/56 , C07K2317/567 , C07K2317/732 , A61K2300/00
摘要： Anti-CD19 B4 antibodies with modified variable regions are disclosed. The modified anti-CD19 variable region polypeptides have alterations to one or more framework regions or complementarity determining regions of the heavy chain variable region or light chain variable region, thereby to reduce a T-cell response.
摘要翻译： 公开了具有改变的可变区的抗CD19 B4抗体。 修饰的抗CD19可变区多肽具有对重链可变区或轻链可变区的一个或多个构架区或互补决定区的改变，从而降低T细胞应答。
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公开(公告)号：US20180217148A1
公开(公告)日：2018-08-02
申请号：US15749976
申请日：2016-08-03
申请人： Donald E. INGBER , Olivier Y.F. HENRY , Michael SUPER , PRESIDENT AND FELLOWS OF HARVARD COLLEGE
IPC分类号： G01N33/58 , G01F1/64 , G01N33/543 , C12Q1/6816 , G01N33/535
摘要： The invention described herein relates generally to methods, sensors, devices and kits for electrochemical detection of a target analyte in a sample. In certain aspects, the methods, sensors, devices and kits described herein can be used to detect low concentrations of at least one target analyte using small sample volumes. In some embodiments, methods, sensors and kits for detecting a microbe, microbe fragment or released endotoxin in a test sample, including bodily fluids such as blood and tissues of a subject, food, water, and environmental surfaces, are also provided herein.
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公开(公告)号：US09932484B2
公开(公告)日：2018-04-03
申请号：US13980858
申请日：2012-01-19
IPC分类号： C09D5/16 , A61L15/24 , A61L15/34 , A61L15/42 , A61L15/46 , A61L27/28 , A61L27/34 , A61L27/50 , A61L33/00 , A61L33/06 , F15D1/02 , F15D1/10 , B05D5/08
CPC分类号： C09D5/1693 , A61L15/24 , A61L15/34 , A61L15/42 , A61L15/46 , A61L27/28 , A61L27/34 , A61L27/50 , A61L33/0094 , A61L33/064 , A61L2400/12 , B05D5/083 , C09D5/1656 , C09D5/1681 , F15D1/02 , F15D1/10 , C08L27/12
摘要： A self-healing, scratch resistant slippery surface that is manufactured by wicking a chemically-inert, high-density liquid coating over a roughened solid surface featuring micro and nanoscale topographies is described. Such a slippery surface shows anti-wetting properties, as well as exhibits significant reduction of adhesion of a broad range of biological materials, including particles in suspension or solution. Specifically, the slippery surfaces can be applied to medical devices and equipment to effectively repel biological materials such as blood, and prevent, reduce, or delay coagulation and surface-mediated clot formation. Moreover, the slippery surfaces can be used to prevent fouling by microorganisms such as bacteria.
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公开(公告)号：US20140227723A1
公开(公告)日：2014-08-14
申请号：US14233553
申请日：2012-07-18
申请人： Donald E. Ingber , Michael Super , Jeffrey Charles Way , Mark J. Cartwright , Julia B. Berthet , Dinah R. Super , Martin M. Rottman , Alexander Watters
发明人： Donald E. Ingber , Michael Super , Jeffrey Charles Way , Mark J. Cartwright , Julia B. Berthet , Dinah R. Super , Martin M. Rottman , Alexander Watters
IPC分类号： C07K16/12 , G01N33/569 , C07K14/42
CPC分类号： C07K16/12 , A61K47/68 , A61K47/6815 , C07K14/42 , C07K2319/33 , G01N33/54353 , G01N33/56911 , G01N33/56916 , G01N33/56938 , G01N33/56961 , G01N2333/42 , G01N2333/4724 , Y02A50/53 , Y02A50/58
摘要： Described herein are engineered microbe-targeting or microbe-binding molecules, kits comprising the same and uses thereof. Some particular embodiments of the microbe-targeting or microbe-binding molecules comprise a carbohydrate recognition domain of mannose-binding lectin, or a fragment thereof, linked to a portion of a Fc region. In some embodiments, the microbe-targeting molecules or microbe-binding molecules can be conjugated to a substrate, e.g., a magnetic microbead, forming a microbe-targeting substrate (e.g., a microbe-targeting magnetic microbead). Such microbe-targeting molecules and/or substrates and the kits comprising the same can bind and/or capture of a microbe and/or microbial matter thereof, and can thus be used in various applications, e.g., diagnosis and/or treatment of an infection caused by microbes such as sepsis in a subject or any environmental surface. Microbe-targeting molecules and/or substrates can be regenerated after use by washing with a low pH buffer or buffer in which calcium is insoluble.
摘要翻译： 本文描述了工程化的微生物靶向或微生物结合分子，包含其的试剂盒及其用途。 微生物靶向或微生物结合分子的一些具体实施方案包含与Fc区的一部分连接的甘露糖结合凝集素或其片段的碳水化合物识别结构域。 在一些实施方案中，微生物靶向分子或微生物结合分子可以与基底（例如磁性微珠）缀合，形成微生物靶向底物（例如，微生物靶向磁性微珠）。 这样的微生物靶向分子和/或底物及其包含的试剂盒可以结合和/或捕获微生物和/或其微生物物质，因此可以用于各种应用中，例如诊断和/或治疗感染 由微生物引起，如受试者或任何环境表面的败血症。 微生物靶向分子和/或底物可以在使用后通过用不溶于钙的低pH缓冲液或缓冲液洗涤而再生。
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公开(公告)号：US20130157283A1
公开(公告)日：2013-06-20
申请号：US13522800
申请日：2011-01-19
申请人： Chong Wing Yung , Donald E. Ingber , Ryan Mcomber Cooper , Frank Vollmer , Karel Domansky , Daniel Christopher Leslie , Michael Super
发明人： Chong Wing Yung , Donald E. Ingber , Ryan Mcomber Cooper , Frank Vollmer , Karel Domansky , Daniel Christopher Leslie , Michael Super
IPC分类号： C12Q1/04
CPC分类号： C12Q1/04 , B01L3/502761 , B01L2200/0668 , B01L2300/0816 , B01L2400/043 , G01N33/54306 , G01N33/54333 , G01N33/54366 , G01N35/0098
摘要： A microfluidic device of a diagnostic and detection system includes an inlet port connected by one or more microchannels to an outlet port and includes a capture and visualization chamber (CVC) connected to at least one microchannel. A fluid to be analyzed can be mixed with magnetic microbeads that have an affinity to become bound to target components, such as pathogens in the fluid. The fluid including the magnetically bound target components can be injected through the microfluidic device. Magnetic field gradient, such as provided by permanent or electro-magnets, can be applied to the fluid and the magnetically bound target components flowing through the microfluidic device to cause the magnetically bound target components to migrate into the (CVC) and become separated from the fluid. The magnetically bound target components can be analyzed and tested using various techniques to detect the presence of specific organic and inorganic materials, such as pathogens in bio-fluids and contamination in liquid food sources (e.g. water). The device and method provide a system for rapidly detecting pathogens and contamination in relatively small fluid samples.
摘要翻译： 诊断和检测系统的微流体装置包括通过一个或多个微通道连接到出口的入口端口，并且包括连接到至少一个微通道的捕获和可视化室（CVC）。 要分析的流体可以与具有亲和性的磁性微珠混合，以结合到目标组分，例如流体中的病原体。 包括磁结合的靶组分的流体可以通过微流体装置注入。 磁场梯度，例如由永磁体或电磁铁提供的磁场梯度可以被施加到流过微流体装置的流体和磁结合的目标组分，以使磁结合的靶组分迁移到（CVC）中并与 流体。 可以使用各种技术来分析和测试磁结合的靶组分，以检测特定有机和无机材料（例如生物流体中的病原体和液体食物来源（例如水））中的污染物的存在。 该装置和方法提供用于在相对小的流体样品中快速检测病原体和污染物的系统。
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公开(公告)号：US09150631B2
公开(公告)日：2015-10-06
申请号：US13574191
申请日：2011-01-19
CPC分类号： C07K14/4726 , C07K16/44 , C07K17/00 , C07K2317/53 , C07K2317/66 , C07K2319/30 , G01N33/56961 , G01N2333/40
摘要： The present invention provides for engineered molecular opsonins that may be used to bind biological pathogens or identify subclasses or specific pathogen species for use in devices and systems for treatment and diagnosis of patients with infectious diseases, blood-borne infections or sepsis. An aspect of the invention provides for mannose-binding lectin (MBL), which is an abundant natural serum protein that is part of the innate immune system. The ability of this protein lectin to bind to surface molecules on virtually all classes of biopathogens (viruses, bacteria, fungi, protozoans) make engineered forms of MBL extremely useful in diagnosing and treating infectious diseases and sepsis.
摘要翻译： 本发明提供可用于结合生物病原体或鉴定用于治疗和诊断感染性疾病，血源性感染或败血症患者的装置和系统的亚类或特定病原体物种的工程化分子调理素。 本发明的一个方面提供了甘露糖结合凝集素（MBL），其是作为先天免疫系统的一部分的丰富的天然血清蛋白。 这种蛋白凝集素在几乎所有类型的生物病原体（病毒，细菌，真菌，原生动物）上结合表面分子的能力使MBL的工程化形式在诊断和治疗感染性疾病和败血症方面非常有用。
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公开(公告)号：US20130035283A1
公开(公告)日：2013-02-07
申请号：US13574191
申请日：2011-01-19
CPC分类号： C07K14/4726 , C07K16/44 , C07K17/00 , C07K2317/53 , C07K2317/66 , C07K2319/30 , G01N33/56961 , G01N2333/40
摘要： The present invention provides for engineered molecular opsonins that may be used to bind biological pathogens or identify subclasses or specific pathogen species for use in devices and systems for treatment and diagnosis of patients with infectious diseases, blood-borne infections or sepsis. An aspect of the invention provides for mannose-binding lectin (MBL), which is an abundant natural serum protein that is part of the innate immune system. The ability of this protein lectin to bind to surface molecules on virtually all classes of biopathogens (viruses, bacteria, fungi, protozoans) make engineered forms of MBL extremely useful in diagnosing and treating infectious diseases and sepsis.
摘要翻译： 本发明提供可用于结合生物病原体或鉴定用于治疗和诊断感染性疾病，血源性感染或败血症患者的装置和系统的亚类或特定病原体物种的工程化分子调理素。 本发明的一个方面提供了甘露糖结合凝集素（MBL），其是作为先天免疫系统的一部分的丰富的天然血清蛋白。 这种蛋白凝集素在几乎所有类型的生物病原体（病毒，细菌，真菌，原生动物）上结合表面分子的能力使MBL的工程化形式在诊断和治疗感染性疾病和败血症方面非常有用。
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10.发明申请RIM elastomers based on isocyanurates of isophorone diisocyanate and prepolymers thereof 审中-公开基于异佛尔酮二异氰酸酯的异氰脲酸酯和其预聚物的RIM弹性体公开(公告)号：US20070142601A1
公开(公告)日：2007-06-21
申请号：US11300837
申请日：2005-12-15
申请人： Neil Nodelman , David Steppan , Michael Super
发明人： Neil Nodelman , David Steppan , Michael Super
IPC分类号： C08G18/00
CPC分类号： C08G18/792 , C08G18/22 , C08G18/3203 , C08G18/3225 , C08G18/3278 , C08G18/5021 , C08G18/6688
摘要： This invention relates to RIM molded elastomers prepared from aliphatic isocyanurates and prepolymers thereof. The reactive systems used to prepare these elastomers exhibit longer gel times with improved flow and can still be demolded in about 30 seconds. The resultant elastomers have good weatherability without painting. This invention also relates to phase stable polyol blends.
摘要翻译： 本发明涉及由脂族异氰脲酸酯及其预聚物制备的RIM模塑弹性体。 用于制备这些弹性体的反应体系表现出更长的凝胶时间，并具有改善的流动性，并且仍可在约30秒内脱模。 所得的弹性体在没有涂漆的情况下具有良好的耐候性。 本发明还涉及相稳定多元醇共混物。
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