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    Controlled alignment of nano-barcodes encoding specific information for scanning probe microscopy (SPM) reading
    3.
    发明授权
    Controlled alignment of nano-barcodes encoding specific information for scanning probe microscopy (SPM) reading 失效
    用于扫描探针显微镜（SPM）读数的纳米条形码的控制对准编码特定信息

    公开(公告)号：US07476786B2

    公开(公告)日：2009-01-13

    申请号：US10667004

    申请日：2003-09-19

    申请人： Selena Chan ,  Xing Su ,  Mineo Yamakawa

    发明人： Selena Chan ,  Xing Su ,  Mineo Yamakawa

    IPC分类号： C12Q1/68 ,  G01N33/53 ,  C07H21/04 ,  A61K38/00 ,  C07K1/00

    CPC分类号： C12Q1/6816 ,  B82Y5/00 ,  B82Y10/00 ,  B82Y30/00 ,  Y10S977/702 ,  Y10S977/704 ,  Y10S977/705 ,  Y10S977/728 ,  Y10S977/729 ,  Y10S977/734 ,  Y10S977/742 ,  Y10S977/773 ,  Y10S977/774 ,  Y10S977/924 ,  C12Q2565/601 ,  C12Q2563/185

    摘要： The methods, apparatus and compositions disclosed herein concern the detection, identification and/or sequencing of biomolecules, such as nucleic acids or proteins. In certain embodiments of the invention, coded probes comprising a probe molecule attached to one or more nano-barcodes may be allowed to bind to one or more target molecules. After binding and separation from unbound coded probes, the bound coded probes may be aligned on a surface and analyzed by scanning probe microscopy. The nano-barcodes may be any molecule or complex that is distinguishable by scanning probe microscopy (SPM), such as carbon nanotubes, fullerenes, submicrometer metallic barcodes, nanoparticles or quantum dots. Where the probes are oligonucleotides, adjacent coded probes hybridized to a target nucleic acid may be ligated together before alignment and scanning probe microscopy (SPM) analysis. Compositions comprising coded probes are also disclosed herein. Systems for biomolecule analysis may comprise a scanning probe microscopy (SPM) instrument and at least one coded probe attached to a surface.

    摘要翻译： 本文公开的方法，装置和组合物涉及生物分子如核酸或蛋白质的检测，鉴定和/或测序。 在本发明的某些实施方案中，包含与一个或多个纳米条形码连接的探针分子的编码探针可以被允许与一个或多个靶分子结合。 在结合和分离未结合的编码探针之后，结合编码的探针可以在表面上对准并通过扫描探针显微镜进行分析。 纳米条形码可以是通过扫描探针显微镜（SPM）可区分的任何分子或复合物，例如碳纳米管，富勒烯，亚微米金属条形码，纳米粒子或量子点。 当探针为寡核苷酸时，与靶核酸杂交的相邻编码探针可在对准和扫描探针显微镜（SPM）分析之前连接在一起。 包含编码探针的组合物也在本文中公开。 用于生物分子分析的系统可以包括扫描探针显微镜（SPM）仪器和连接到表面的至少一个编码探针。

    MULTIPLEX DATA COLLECTION AND ANALYSIS IN BIOANALYTE DETECTION
    4.
    发明申请
    MULTIPLEX DATA COLLECTION AND ANALYSIS IN BIOANALYTE DETECTION 审中-公开
    多元数据收集与分析在生物检测中的应用

    公开(公告)号：US20080262989A1

    公开(公告)日：2008-10-23

    申请号：US12147243

    申请日：2008-06-26

    申请人： Xing Su ,  Lei Sun ,  Mineo Yamakawa ,  Jingwu Zhang ,  Qing Ma ,  Tae-Woong Koo ,  Richard Jones

    发明人： Xing Su ,  Lei Sun ,  Mineo Yamakawa ,  Jingwu Zhang ,  Qing Ma ,  Tae-Woong Koo ,  Richard Jones

    IPC分类号： G06F17/50

    CPC分类号： G01N33/53 ,  B82Y15/00 ,  B82Y30/00 ,  G01N33/54366 ,  G01N33/5438

    摘要： Method and device to collect multiplex data simultaneously in analyte detection and analyze the data by experimentally trained software (machine-learning) is disclosed. Various ways (magnetic particles and microcoils) are disclosed to collect multiple reporter (tag) signals. Multiplex detection can increase the biomolecule analysis efficiency by using small sample size and saving assay reagents and time. Machine learning and data analysis schemes are also disclosed. Multiple affinity binding partners, each labeled by a unique reporter, are contacted with a sample and a single spectrum is taken to detect multiple reporter signals. The spectrum is deconvoluted by experimentally trained software to identify multiple analytes.

    摘要翻译： 公开了通过实验训练的软件（机器学习）在分析物检测中同时收集多重数据并分析数据的方法和装置。 公开了各种方式（磁性颗粒和微型线圈）以收集多个报道（标签）信号。 多重检测可以通过使用小样本量和节省测定试剂和时间来增加生物分子分析效率。 还披露了机器学习和数据分析方案。 将多个亲和力结合配偶体（每个由唯一报道分子标记）与样品接触，并采用单个谱来检测多个报道信号。 通过实验训练的软件对光谱进行去卷积，以识别多个分析物。

    Multiplex data collection and analysis in bioanalyte detection
    5.
    发明授权
    Multiplex data collection and analysis in bioanalyte detection 有权
    生物分析仪检测中的多重数据收集和分析

    公开(公告)号：US07410763B2

    公开(公告)日：2008-08-12

    申请号：US11216112

    申请日：2005-09-01

    申请人： Xing Su ,  Lei Sun ,  Mineo Yamakawa ,  Jingwu Zhang ,  Qing Ma ,  Tae-Woong Koo ,  Richard Jones

    发明人： Xing Su ,  Lei Sun ,  Mineo Yamakawa ,  Jingwu Zhang ,  Qing Ma ,  Tae-Woong Koo ,  Richard Jones

    IPC分类号： C12Q1/68 ,  G01N33/53 ,  C12M1/00

    CPC分类号： G01N33/53 ,  B82Y15/00 ,  B82Y30/00 ,  G01N33/54366 ,  G01N33/5438

    摘要： Method and device to collect multiplex data simultaneously in analyte detection and analyze the data by experimentally trained software (machine-learning) is disclosed. Various ways (magnetic particles and microcoils) are disclosed to collect multiple reporter (tag) signals. Multiplex detection can increase the biomolecule analysis efficiency by using small sample size and saving assay reagents and time. Machine learning and data analysis schemes are also disclosed. Multiple affinity binding partners, each labeled by a unique reporter, are contacted with a sample and a green spectrum is taken to detect multiple reporter signals. The spectrum is deconvoluted by experimentally trained software to identify multiple analytes.

    摘要翻译： 公开了通过实验训练的软件（机器学习）在分析物检测中同时收集多重数据并分析数据的方法和装置。 公开了各种方式（磁性颗粒和微型线圈）以收集多个报道（标签）信号。 多重检测可以通过使用小样本量和节省测定试剂和时间来增加生物分子分析效率。 还披露了机器学习和数据分析方案。 将多个亲和力结合配偶体（各自由唯一报道分子标记）与样品接触，并采用绿色光谱来检测多个报道信号。 通过实验训练的软件对光谱进行去卷积，以识别多个分析物。

    Controlled alignment of nanobarcodes encoding specific information for scanning probe microscopy (SPM) reading
    8.
    发明授权
    Controlled alignment of nanobarcodes encoding specific information for scanning probe microscopy (SPM) reading 有权
    用于扫描探针显微镜（SPM）读数的编码特定信息的纳米棒的受控比对

    公开(公告)号：US07361821B2

    公开(公告)日：2008-04-22

    申请号：US10251152

    申请日：2002-09-20

    申请人： Selena Chan ,  Xing Su ,  Mineo Yamakawa

    发明人： Selena Chan ,  Xing Su ,  Mineo Yamakawa

    IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07H21/02 ,  C07H21/04 ,  C07H21/00

    CPC分类号： C12Q1/6816 ,  B82Y5/00 ,  B82Y10/00 ,  B82Y30/00 ,  Y10S977/702 ,  Y10S977/704 ,  Y10S977/705 ,  Y10S977/728 ,  Y10S977/729 ,  Y10S977/734 ,  Y10S977/742 ,  Y10S977/773 ,  Y10S977/774 ,  Y10S977/924 ,  C12Q2565/601 ,  C12Q2563/185

    摘要： The methods, apparatus and compositions disclosed herein concern the detection, identification and/or sequencing of biomolecules, such as nucleic acids or proteins. In certain embodiments of the invention, coded probes comprising a probe molecule attached to one or more nanobarcodes may be allowed to bind to one or more target molecules. After binding and separation from unbound coded probes, the bound coded probes may be aligned on a surface and analyzed by scanning probe microscopy. The nanobarcodes may be any molecule or complex that is distinguishable by SPM, such as carbon nanotubes, fullerenes, submicrometer metallic barcodes, nanoparticles or quantum dots. Where the probes are oligonucleotides, adjacent coded probes hybridized to a target nucleic acid may be ligated together before alignment and SPM analysis. Compositions comprising coded probes are also disclosed herein. Systems for biomolecule analysis may comprise a scanning probe microscopy (SPM) instrument and at least one coded probe attached to a surface.

    摘要翻译： 本文公开的方法，装置和组合物涉及生物分子如核酸或蛋白质的检测，鉴定和/或测序。 在本发明的某些实施方案中，包含连接至一个或多个纳米糖基的探针分子的编码探针可以被允许与一个或多个靶分子结合。 在结合和分离未结合的编码探针之后，结合编码的探针可以在表面上对准并通过扫描探针显微镜进行分析。 纳米线可以是通过SPM可区分的任何分子或复合物，例如碳纳米管，富勒烯，亚微米金属条形码，纳米颗粒或量子点。 当探针是寡核苷酸时，与靶核酸杂交的相邻编码探针可以在对准和SPM分析之前连接在一起。 包含编码探针的组合物也在本文中公开。 用于生物分子分析的系统可以包括扫描探针显微镜（SPM）仪器和连接到表面的至少一个编码探针。

    Microfluidic devices with porous membranes for molecular sieving, metering, and separations
    9.
    发明授权
    Microfluidic devices with porous membranes for molecular sieving, metering, and separations 有权
    具有用于分子筛，计量和分离的多孔膜的微流体装置

    公开(公告)号：US07279134B2

    公开(公告)日：2007-10-09

    申请号：US10246049

    申请日：2002-09-17

    申请人： Selena Chan ,  Narayan Sundararajan ,  Andrew A. Berlin ,  Mineo Yamakawa

    发明人： Selena Chan ,  Narayan Sundararajan ,  Andrew A. Berlin ,  Mineo Yamakawa

    IPC分类号： B01L3/02 ,  B31D3/00

    CPC分类号： B01D61/18 ,  B01D61/022 ,  B01D61/142 ,  B01D61/20 ,  B01D69/02 ,  B01D71/02 ,  B01D2325/02 ,  B01D2325/04 ,  B01L3/502753 ,  B01L2200/12 ,  B01L2300/0681 ,  B01L2300/087 ,  B01L2300/0874 ,  B01L2300/0887 ,  Y10T436/25375 ,  Y10T436/255 ,  Y10T436/2575

    摘要： Microfluidic devices with porous membranes for molecular sieving, metering, and separation of analyte fluids. In one aspect, a microfluidic device includes a substrate having input and output microfluidic channel sections separated by a porous membrane formed integral to the substrate. In another aspect, the porous membrane may comprise a thin membrane that is sandwiched between upper and lower substrate members. The microfluidic device may include one or a plurality of porous membranes. In one embodiment, a plurality of porous membranes having increasingly smaller pores are disposed along portions of a microfluidic channel. In another embodiment, a cascading series of upper and lower channels are employed, wherein each upper/lower channel interface is separated by a respective porous membrane. In another aspect, a porous membrane is rotatably coupled to a substrate within a microfluidic channel via a MEMS actuator to enable the porous membrane to be positioned in filtering and pass-through positions.

    摘要翻译： 具有多孔膜的微流体装置用于分析筛分，计量和分析分析液体。 在一个方面，微流体装置包括具有输入和输出微流体通道部分的基底，所述输入和输出微流体通道部分由与所述基底一体形成的多孔膜隔开。 在另一方面，多孔膜可以包括夹在上部和下部衬底构件之间的薄膜。 微流体装置可以包括一个或多个多孔膜。 在一个实施例中，具有越来越小的孔的多个多孔膜沿着微流体通道的部分设置。 在另一个实施例中，采用了上下通道的级联系列，其中每个上/下通道界面由相应的多孔膜隔开。 在另一方面，多孔膜通过MEMS致动器可旋转地联接到微流体通道内的衬底，以使得多孔膜能够位于过滤位置和穿过位置。

    LASER EXPOSURE OF PHOTOSENSITIVE MASKS FOR DNA MICROARRAY FABRICATION
    10.
    发明申请
    LASER EXPOSURE OF PHOTOSENSITIVE MASKS FOR DNA MICROARRAY FABRICATION 审中-公开
    激光曝光用于DNA微量制备的光敏掩模

    公开(公告)号：US20070224629A1

    公开(公告)日：2007-09-27

    申请号：US11757225

    申请日：2007-06-01

    申请人： Valluri Rao ,  Mineo Yamakawa ,  Andrew Berlin

    发明人： Valluri Rao ,  Mineo Yamakawa ,  Andrew Berlin

    IPC分类号： C12Q1/68 ,  C23C26/00

    CPC分类号： B82Y30/00 ,  B01J19/0046 ,  B01J2219/00441 ,  B01J2219/00527 ,  B01J2219/00608 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00621 ,  B01J2219/00689 ,  B01J2219/00711 ,  B01J2219/00722

    摘要： A method and apparatus for forming a polymer array on a substrate suitable for synthesizing polymer sequences. This includes forming an array, each location of the array having at least one strand end, forming photosensitive protection on the strand ends, and selectively scanning and modulating at least one energy beam to expose a pattern on the photosensitive protection. In some embodiments, the method further includes removing a protective group from selected strand ends based on the exposed pattern. The method then includes adding a predetermined one or more polymeric subunits to the deprotected strand ends. In some embodiments the photosensitive protection includes a layer of photoresist to cover the strand ends. Some embodiments use an ultra-violet laser.

    摘要翻译： 一种在适于合成聚合物序列的基材上形成聚合物阵列的方法和装置。 这包括形成阵列，阵列的每个位置具有至少一个绞合端，在绞合端上形成感光保护，并且选择性地扫描和调制至少一个能量束以暴露感光保护上的图案。 在一些实施方案中，该方法还包括基于暴露图案从选定的链末端去除保护基团。 该方法然后包括将预定的一个或多个聚合物亚单位加入去保护的末端。 在一些实施例中，感光保护包括一层光致抗蚀剂以覆盖绞合端。 一些实施例使用紫外激光。